5cro

REFINED STRUCTURE OF CRO REPRESSOR PROTEIN FROM BACTERIOPHAGE LAMBDA

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

CRO REPRESSOR PROTEIN

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	PHOSPHATE ION × 2 water × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
3	Protein homooligomer	Homooligomer	Protein 24	PHOSPHATE ION × 12 water × 24	Consistent with protein count
4	Protein homooligomer	Homooligomer	Protein 4	PHOSPHATE ION × 2 water × 4	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RCRO_LAMBD
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–66； UniProt 1–66 Author chain B； PDBConstruct 1–66； UniProt 1–66 Author chain C； PDBConstruct 1–66； UniProt 1–66 Author chain O； PDBConstruct 1–66； UniProt 1–66

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5cro
Deposition date deposition_date	1998-04-17
Structure title title	REFINED STRUCTURE OF CRO REPRESSOR PROTEIN FROM BACTERIOPHAGE LAMBDA
Keywords keywords	GENE REGULATING PROTEIN, TRANSCRIPTION REGULATION; GENE REGULATING PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5cro__assembly_2__model_1

Assembly 2 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5cro__assembly_2__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5cro__assembly_2__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	17.42 Å
Rg (electron density)	16.52 Å
Total Rg	17.47 Å
Atom count	949
Residues	122
Excluded volume	17022 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5cro__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`5cro__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
3	1	`5cro__assembly_3__model_1`	24-meric （24）	Success	4.1.3-1-20251215 (887e7ef)	View Download
4	1	`5cro__assembly_4__model_1`	tetrameric （4）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

Domain ID domain_id	d5croa_
Class class	a — All alpha proteins
Fold Fold fold	a.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
Family Family family	a.35.1.2 — Phage repressors

Domain ID domain_id	d5crob_
Class class	a — All alpha proteins
Fold Fold fold	a.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
Family Family family	a.35.1.2 — Phage repressors

Domain ID domain_id	d5croc_
Class class	a — All alpha proteins
Fold Fold fold	a.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
Family Family family	a.35.1.2 — Phage repressors

Domain ID domain_id	d5croo_
Class class	a — All alpha proteins
Fold Fold fold	a.35 — lambda repressor-like DNA-binding domains
Superfamily Superfamily superfamily	a.35.1 — lambda repressor-like DNA-binding domains
Family Family family	a.35.1.2 — Phage repressors

CATH v4.4 (4 domains)

Domain ID domain_id	5croA00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	240 — CRO Repressor
Homologous superfamily homologous superfamily	10 — CRO Repressor

Domain ID domain_id	5croB00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	240 — CRO Repressor
Homologous superfamily homologous superfamily	10 — CRO Repressor

Domain ID domain_id	5croC00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	240 — CRO Repressor
Homologous superfamily homologous superfamily	10 — CRO Repressor

Domain ID domain_id	5croO00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	240 — CRO Repressor
Homologous superfamily homologous superfamily	10 — CRO Repressor

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7. Citations (19)

Refined structure of Cro repressor protein from bacteriophage lambda suggests both flexibility and plasticity.

J.Mol.Biol. (1998)

PMID: 9653036 · DOI: 10.1006/jmbi.1998.1849 PRIMARY

Different Interactions Used by Cro Repressor in Specific and Nonspecific DNA Binding

J.Biol.Chem. (1986)

Use of Protein Sequence and Structure to Infer Distant Evolutionary Relationships

Chem.Scr. (1986)

The Structure of Cro Repressor Protein

Biological Macromolecules and Assemblies. V.2: Nucleic Acids and Interactive Proteins (1985)

Cro Repressor Protein and its Interaction with DNA

Cold Spring Harbor Symp.Quant.Biol. (1983)

Many Gene-Regulatory Proteins Appear to Have a Similar Alpha-Helical Fold that Binds DNA and Evolved from a Common Precursor

J.Mol.Evol. (1983)

High Resolution Structural Studies of Cro Repressor Protein and Implications for DNA Recognition

J.Biomol.Struct.Dyn. (1983)

DNA-Binding Proteins

Science (1983)

Comparison of the Structures of Cro and Lambda Repressor Proteins from Bacteriophage Lambda

J.Mol.Biol. (1983)

Crystallographic Data for Complexes of the Cro Repressor with DNA

J.Mol.Biol. (1983)

Structural Studies of Protein-Nucleic Acid Interactions

Annu.Rev.Biophys.Bioeng. (1983)

How Does Cro Repressor Recognize its DNA Target Sites?

Trends Biochem.Sci. (1983)

The Molecular Basis of DNA-Protein Recognition Inferred from the Structure of Cro Repressor

Nature (1982)

Structural Similarity in the DNA-Binding Domains of Catabolite Gene Activator and Cro Repressor Proteins

Proc.Natl.Acad.Sci.USA (1982)

Structure of the DNA-Binding Region of Lac Repressor Inferred from its Homology with Cro Repressor

Proc.Natl.Acad.Sci.USA (1982)

Proposed Alpha-Helical Super-Secondary Structure Associated with Protein-DNA Recognition

J.Mol.Biol. (1982)

Structure of the Cro Repressor from Bacteriophage Lambda and its Interaction with DNA

Nature (1981)

The Structure of a Repressor. Crystallographic Data for the Cro Regulatory Protein of Bacteriophage Lambda

J.Mol.Biol. (1979)

Amino Acid Sequence of Cro Regulatory Protein of Bacteriophage Lambda

Nature (1977)

Unit-cell length a length_a	91.600 Å
Unit-cell length b length_b	91.600 Å
Unit-cell length c length_c	268.500 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	120.00 °
Molecules per unit cell Z z_pdb	72

Space group space_group	H 3 2
Space-group number int_tables_number	155
Crystal system crystal_system	—

Resolution resolution	2.300 Å
Low-resolution limit resolution_low	20.000 Å
R factor r_factor	—
R_work r_work	0.1930
R_free r_free	—

Description description	CRO REPRESSOR PROTEIN
Molecular weight formula_weight	7375.5 kDa
Source method src_method	nat
Copy count entity_count	4

Polymer type type	polypeptide(L)
Chain IDs strand_id	O,A,B,C
Atom count atom_count	1940

REFINED STRUCTURE OF CRO REPRESSOR PROTEIN FROM BACTERIOPHAGE LAMBDA

1. Protein Identity and Related Structures Protein Identity & Related Structures

CRO REPRESSOR PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5cro__assembly_2__model_1

5cro__assembly_2__model_1 | I(q)

5cro__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

CATH v4.4 (4 domains)

7. Citations (19)

Description description	PHOSPHATE ION
Molecular weight formula_weight	95.0 kDa
Source method src_method	syn
Copy count entity_count	2

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	33

REFINED STRUCTURE OF CRO REPRESSOR PROTEIN FROM BACTERIOPHAGE LAMBDA

1. Protein Identity and Related Structures Protein Identity & Related Structures

CRO REPRESSOR PROTEIN

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5cro__assembly_2__model_1

5cro__assembly_2__model_1 | I(q)

5cro__assembly_2__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 water

6. Fold Classification (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

CATH v4.4 (4 domains)

7. Citations (19)