← Back to search
5do9

Structure of regulator of G protein signaling 8 (RGS8) in complex with AlF4-activated Galpha-q

Method: X-RAY DIFFRACTION
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Guanine nucleotide-binding protein G(q) subunit alpha

Mus musculus

UniProt P21279

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Regulator of G-protein signaling 8 × 1 （P57771） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2bcj 3ah8 4ekc 4ekc 4ekd 4gnk 4gnk 4qj3 4qj4 4qj5 5do9 5do9

17 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name GNAQ_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–314； UniProt 37–350 Author chain C； PDBConstruct 1–314； UniProt 37–350 Author chain E； PDBConstruct 1–314； UniProt 37–350

Regulator of G-protein signaling 8

Homo sapiens

UniProt P57771

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Guanine nucleotide-binding protein G(q) subunit alpha × 1 （P21279） GUANOSINE-5'-DIPHOSPHATE × 1 TETRAFLUOROALUMINATE ION × 1 MAGNESIUM ION × 1 water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
2ihd

1 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
2ode 2ode 5do9 5do9 5do9 5do9 5do9 5do9

8 assemblies

View Construct and Data Evidence
UniProt name RGS8_HUMAN
Isoform P57771-2
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 3–134； UniProt 60–191 Author chain D； PDBConstruct 3–134； UniProt 60–191 Author chain F； PDBConstruct 3–134； UniProt 60–191

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id5do9
Deposition date deposition_date2015-09-10
Structure title titleStructure of regulator of G protein signaling 8 (RGS8) in complex with AlF4-activated Galpha-q
Keywords keywordsGTP-Binding Protein alpha subunits, Gq-G11, RGS proteins, PROTEIN BINDING; PROTEIN BINDING
Experimental Method methodX-RAY DIFFRACTION
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5do9__assembly_3__model_1

Assembly 3 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5do9__assembly_3__model_1 | I(q)

10-2 10-1 105 106 107 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5do9__assembly_3__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）23.77 Å
Rg (electron density)22.86 Å
Total Rg23.75 Å
Atom count3724
Residues447
Excluded volume66195 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5do9__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 5do9__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 5do9__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 5do9__assembly_4__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 5do9__assembly_5__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 5do9__assembly_6__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (6)

▼

6. Fold Classification (SCOP + CATH) 18 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd5do9b1
Class classa — All alpha proteins
Fold Fold folda.91 — Regulator of G-protein signaling, RGS
Superfamily Superfamily superfamilya.91.1 — Regulator of G-protein signaling, RGS
Family Family familya.91.1.0 — automated matches
Domain ID domain_idd5do9b2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd5do9d1
Class classa — All alpha proteins
Fold Fold folda.91 — Regulator of G-protein signaling, RGS
Superfamily Superfamily superfamilya.91.1 — Regulator of G-protein signaling, RGS
Family Family familya.91.1.0 — automated matches
Domain ID domain_idd5do9d2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags
Domain ID domain_idd5do9f1
Class classa — All alpha proteins
Fold Fold folda.91 — Regulator of G-protein signaling, RGS
Superfamily Superfamily superfamilya.91.1 — Regulator of G-protein signaling, RGS
Family Family familya.91.1.0 — automated matches
Domain ID domain_idd5do9f2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (12 domains)

Domain ID domain_id5do9A01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id5do9A02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology400 — GI Alpha 1, domain 2-like
Homologous superfamily homologous superfamily10 — GI Alpha 1, domain 2-like
Domain ID domain_id5do9B01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology196 — Regulator of G-protein Signalling 4; domain 1
Homologous superfamily homologous superfamily10 —
Domain ID domain_id5do9B02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology167 — Regulator of G-protein Signalling 4; domain 2
Homologous superfamily homologous superfamily10 — Regulator of G-protein Signalling 4, domain 2
Domain ID domain_id5do9C01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id5do9C02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology400 — GI Alpha 1, domain 2-like
Homologous superfamily homologous superfamily10 — GI Alpha 1, domain 2-like
Domain ID domain_id5do9D01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology196 — Regulator of G-protein Signalling 4; domain 1
Homologous superfamily homologous superfamily10 —
Domain ID domain_id5do9D02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology167 — Regulator of G-protein Signalling 4; domain 2
Homologous superfamily homologous superfamily10 — Regulator of G-protein Signalling 4, domain 2
Domain ID domain_id5do9E01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
Domain ID domain_id5do9E02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology400 — GI Alpha 1, domain 2-like
Homologous superfamily homologous superfamily10 — GI Alpha 1, domain 2-like
Domain ID domain_id5do9F01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology196 — Regulator of G-protein Signalling 4; domain 1
Homologous superfamily homologous superfamily10 —
Domain ID domain_id5do9F02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology167 — Regulator of G-protein Signalling 4; domain 2
Homologous superfamily homologous superfamily10 — Regulator of G-protein Signalling 4, domain 2
▶

7. Citations (1)