Green fluorescent protein
Aequorea victoria
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
| 2 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain B； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
| 3 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain C； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
| 4 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain D； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
| 5 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain E； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
| 6 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain F； UniProt 1–238 | Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, D133(4CF), N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No other associated polymer | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；0.2 M potassium sodium formate, 30% PEG 3350 | Resolution 2.54 Å R-free 0.243 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5DPI | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 28QL GFP bound to distal DARPin (AHIR dodecamer scaffold system) Deposited 2026-02-13 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–229（227 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.72 Å |
| 5BT0 Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–234（232 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
|
Resolution 2.03 Å R-free 0.204 |
| 5BT0 Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–234（232 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
|
Resolution 2.03 Å R-free 0.204 |
| 5BTT Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–231（229 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 5 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
|
Resolution 2.14 Å R-free 0.207 |
| 5BTT Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position. Deposited 2015-06-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–231（229 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；297 K；50 mM MMT, 2.5 M (NH4)2SO4
|
Resolution 2.14 Å R-free 0.207 |
| 5DPG sfGFP mutant - 133 p-cyano-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(4CF), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 10 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；22% PEG 3350, 2% Tacsimate pH 6, 0.1 M Bis-Tris pH 6.5
|
Resolution 1.85 Å R-free 0.192 |
| 5DPH sfGFP mutant - 149 p-cyano-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CO2 CARBON DIOXIDE × 1 MG MAGNESIUM ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris pH 8.5, 20% PEG 4000, 0.21 M MgCl2-6H2O
|
Resolution 1.42 Å R-free 0.208 |
| 5DPH sfGFP mutant - 149 p-cyano-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, A206V, N149(4CF) Non-standard monomer：Yes (specific site not provided by mmCIF) | CO2 CARBON DIOXIDE × 1 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris pH 8.5, 20% PEG 4000, 0.21 M MgCl2-6H2O
|
Resolution 1.42 Å R-free 0.208 |
| 5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
|
Resolution 2.50 Å R-free 0.244 |
| 5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
|
Resolution 2.50 Å R-free 0.244 |
| 5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
|
Resolution 2.50 Å R-free 0.244 |
| 5DPJ sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine Deposited 2015-09-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–238（238 aa）
|
Mutation：M1V, R2S, S30R, T65(CRO), Y66(CRO), G67(CRO), A72S, Q80R, D133(5DW), N149(5DW), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；22% PEG 3350 and 0.20 M sodium citrate tribasic, pH 7
|
Resolution 2.50 Å R-free 0.244 |
| 5DY6 Enhanced superfolder GFP with DBCO at 148 Deposited 2015-09-24 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
5–232（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；294 K；0.1 M PCTP Buffer, 15% PEG 1500
|
Resolution 2.66 Å R-free 0.290 |
| 5DY6 Enhanced superfolder GFP with DBCO at 148 Deposited 2015-09-24 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
5–232（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；294 K；0.1 M PCTP Buffer, 15% PEG 1500
|
Resolution 2.66 Å R-free 0.290 |
| 5EHU sfGFP mutant with unnatural amino acid 4-azidoethoxy-L-phenylalanine incorporated at the 149 site Deposited 2015-10-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
2–238（237 aa）
|
Mutation：S30R, A72S, Q80R, N149(4LZ), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；A 40 mg/mL solution of sfGFP-149-AePhe in a 20 mM Hepes buffer pH 7.5 was combined with a precipitation solution (20% PEG 8000, 100 mM Hepes pH 7.5) in a 1:1 ratio to form crystals in a sitting drop well at room temperature
|
Resolution 1.45 Å R-free 0.208 |
| 5EHU sfGFP mutant with unnatural amino acid 4-azidoethoxy-L-phenylalanine incorporated at the 149 site Deposited 2015-10-28 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
2–238（237 aa）
|
Mutation：S30R, A72S, Q80R, N149(4LZ), A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；A 40 mg/mL solution of sfGFP-149-AePhe in a 20 mM Hepes buffer pH 7.5 was combined with a precipitation solution (20% PEG 8000, 100 mM Hepes pH 7.5) in a 1:1 ratio to form crystals in a sitting drop well at room temperature
|
Resolution 1.45 Å R-free 0.208 |
| 5JZK The Structure of Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–238（236 aa）
Fragment：UNP residues 3-238
Chain B
3–238（236 aa）
Fragment：UNP residues 3-238
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 4 EDO 1,2-ETHANEDIOL × 2 CL CHLORIDE ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；281 K；20 % w/v PEG 3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium nitrate
|
Resolution 1.90 Å R-free 0.176 |
| 5JZL The Structure of Monomeric Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
Fragment：UNP residues 3-238
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；281 K；22% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M phosphate-citrate buffer pH 3.7, 4% v/v acetone
|
Resolution 1.80 Å R-free 0.217 |
| 5JZL The Structure of Monomeric Ultra Stable Green Fluorescent Protein Deposited 2016-05-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
Fragment：UNP residues 3-238
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；281 K；22% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M phosphate-citrate buffer pH 3.7, 4% v/v acetone
|
Resolution 1.80 Å R-free 0.217 |
| 5MFC Designed armadillo repeat protein YIIIM5AII in complex with (KR)4-GFP Deposited 2016-11-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；8% w/v PEG 4000, 0.1M Na acetate trihydrate pH 4.6,
|
Resolution 2.40 Å R-free 0.240 |
| 5MFC Designed armadillo repeat protein YIIIM5AII in complex with (KR)4-GFP Deposited 2016-11-18 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4.6；277 K；8% w/v PEG 4000, 0.1M Na acetate trihydrate pH 4.6,
|
Resolution 2.40 Å R-free 0.240 |
| 5NHN Super-Folder Green Fluorescent Protein Artificiall dimer linked via 148 position Deposited 2017-03-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–231（229 aa）
Chain B
3–231（229 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；300 K；Condition C6 of the PACT Premier screen:
0.1 M PCTP Buffer, pH 9.0, 25% PEG 1500
|
Resolution 1.96 Å R-free 0.209 |
| 5NI3 sfGFP 204-204 mutant dimer Deposited 2017-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–229（227 aa）
Chain D
3–233（231 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | NA SODIUM ION × 6 DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PACT condition C6: 0.1M PCTP Buffer, pH 9.0, 25% PEG 1500
|
Resolution 1.28 Å R-free 0.191 |
| 5NI3 sfGFP 204-204 mutant dimer Deposited 2017-03-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–233（231 aa）
Chain C
3–233（231 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | NA SODIUM ION × 4 DB5 [(4~{S})-4,5,6,7,8,9-hexahydro-1~{H}-cycloocta[d][1,2,3]triazol-4-yl] hydrogen carbonate × 1 GOL GLYCEROL × 4 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；PACT condition C6: 0.1M PCTP Buffer, pH 9.0, 25% PEG 1500
|
Resolution 1.28 Å R-free 0.191 |
| 5YR2 Structure of cpGFP66BPA Deposited 2017-11-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
146–235（90 aa）
Chain A
3–144（142 aa）
|
Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；289.15 K；PEG 3350 25%, 0.2M MgCl2,0.1M Bis-Tris pH 5.5
|
Resolution 1.80 Å R-free 0.215 |
| 5YR2 Structure of cpGFP66BPA Deposited 2017-11-08 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
146–235（90 aa）
Chain B
3–144（142 aa）
|
Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：Y163(BPA) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；289.15 K；PEG 3350 25%, 0.2M MgCl2,0.1M Bis-Tris pH 5.5
|
Resolution 1.80 Å R-free 0.215 |
| 5Z6Y Structure of sfYFP48S95C66BPA Deposited 2018-01-25 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–230（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 4；289 K；0.1M sodium malonate pH 4.0, 12% PEG 3350
|
Resolution 1.87 Å R-free 0.236 |
| 6B9C Superfolder Green Fluorescent Protein with 4-nitro-L-phenylalanine at the chromophore (position 66) Deposited 2017-10-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Mutation：S30R, A72S, Q80R, A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | CO2 CARBON DIOXIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1.4 M DL-Malic acid
|
Resolution 1.70 Å R-free 0.195 |
| 6B9C Superfolder Green Fluorescent Protein with 4-nitro-L-phenylalanine at the chromophore (position 66) Deposited 2017-10-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–238（236 aa）
|
Mutation：S30R, A72S, Q80R, A206V Non-standard monomer：Yes (specific site not provided by mmCIF) | CO2 CARBON DIOXIDE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1.4 M DL-Malic acid
|
Resolution 1.70 Å R-free 0.195 |
| 6H01 Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66 Deposited 2018-07-06 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–238（236 aa）
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；1.1 M tri-Na-citrate
0.1 M Na-HEPES
|
Resolution 2.78 Å R-free 0.239 |
| 6H01 Crystal structure of a domain-swapped dark-state sfGFP containing the unnatural amino acid ortho-nitrobenzyl-tyrosine (ONBY) at residue 66 Deposited 2018-07-06 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
3–238（236 aa）
Chain D
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；291 K；1.1 M tri-Na-citrate
0.1 M Na-HEPES
|
Resolution 2.78 Å R-free 0.239 |
| 6HUT GFP8 - a stabilized variant of cycle-3 GFP Deposited 2018-10-09 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–245（245 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；296 K；200 mM HEPES, 10% v/v isopropanol, 200 mM NaCl
|
Resolution 1.29 Å R-free 0.203 |
| 6KRG Crystal structure of sfGFP Y182TMSiPhe Deposited 2019-08-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–236（236 aa）
|
Mutation：Y182T Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 2 CL CHLORIDE ION × 3 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；289 K；PEG 6000, SODIUM CHLORIDE
|
Resolution 1.40 Å R-free 0.173 |
| 6OA8 Superfolder Green Fluorescent Protein with 4-cyano-L-phenylalanine at the chromophore (position 66) Deposited 2019-03-15 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 8 NA SODIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；0.1 M Tris-HCl, 2.0 M ammonium sulfate, pH 8.5
|
Resolution 1.37 Å R-free 0.189 |
| 6UN5 Crystal structure of green fluorescent protein (GFP); S65T, Y66(2,3,5-F3Y); ih circular permutant (50-51) Deposited 2019-10-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–237（187 aa）
Chain B
51–237（187 aa）
|
Mutation：;A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S,A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V ; Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：;A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S,A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V ; Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 31% PEG 3350
|
Resolution 1.36 Å R-free 0.205 |
| 6UN6 Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-NO2Y); ih circular permutant (50-51) Deposited 2019-10-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–237（187 aa）
Chain B
51–237（187 aa）
|
Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 34% PEG 3350
|
Resolution 1.50 Å R-free 0.181 |
| 6UN7 Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-OMeY); ih circular permutant (50-51) Deposited 2019-10-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
51–237（187 aa）
Chain B
51–237（187 aa）
|
Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：A72S, Q80R, T105K, E111V, I128T, K166T, I167V, S205T, A206V, S232R, Y241I, C250S Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.15 M ammonium acetate, 34% PEG 3350
|
Resolution 1.50 Å R-free 0.191 |
| 6WV3 Human VKOR with warfarin Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 SWF S-WARFARIN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；37% PEG400, 0.1 M MES, pH 6.5
|
Resolution 2.20 Å R-free 0.223 |
| 6WV4 Human VKOR C43S with warfarin Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) | SWF S-WARFARIN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；25-33% PEG400, 185-220 mM potassium formate, 0.1 M MES, pH 6.5
|
Resolution 3.01 Å R-free 0.292 |
| 6WV5 Human VKOR C43S mutant with vitamin K1 epoxide Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：C43S Non-standard monomer：Yes (specific site not provided by mmCIF) | UAV (2R,3R)-2-hydroxy-3-methyl-2-[(2E,7S)-3,7,11,15-tetramethylhexadec-2-en-1-yl]-2,3-dihydronaphthalene-1,4-dione × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；25% PEG400, 0.1 M ammonium citrate dibasic, 0.1 M MES, pH 6.5
|
Resolution 2.80 Å R-free 0.270 |
| 6WV6 Human VKOR with phenindione Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 UAS Phenindione × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；34% PEG400, 80 mM lithium sulfate, 0.1 M MES, pH 6.5
|
Resolution 2.70 Å R-free 0.268 |
| 6WV7 Human VKOR with Chlorophacinone Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | UAJ Chlorophacinone × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；33% PEG400, 5% DMSO, 0.1 M sodium acetate, 0.1 M MES, pH 6.5
|
Resolution 2.48 Å R-free 0.245 |
| 6WV7 Human VKOR with Chlorophacinone Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain B
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | UAJ Chlorophacinone × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；33% PEG400, 5% DMSO, 0.1 M sodium acetate, 0.1 M MES, pH 6.5
|
Resolution 2.48 Å R-free 0.245 |
| 6WV8 Takifugu rubripes VKOR-like C138S mutant with vitamin K1 Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
146–238（93 aa）
|
Mutation：C138S Non-standard monomer：Yes (specific site not provided by mmCIF) | PQN PHYLLOQUINONE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6；295 K；25% PEG 400, 300 mM ammonium fluoride, 0.1 M MES pH 6.0
|
Resolution 3.01 Å R-free 0.293 |
| 6WVB Takifugu rubripes VKOR-like with warfarin Deposited 2020-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
146–238（93 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | SWF S-WARFARIN × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 5.5；295 K；23% PEG400, 0.1 M Sodium nitrate, 0.1 M MES pH 5.5
|
Resolution 2.87 Å R-free 0.286 |
| 6WVH Human VKOR with Brodifacoum Deposited 2020-05-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain A
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | UA7 Brodifacoum × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；32% PEG400, 0.1 M ammonium acetate, 1% 1,2,3-heptanetriol, 0.1 M MES, pH 6.5
|
Resolution 1.99 Å R-free 0.215 |
| 6WVH Human VKOR with Brodifacoum Deposited 2020-05-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–144（144 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
Chain B
146–231（86 aa）
Fragment：GPF (UNP residues 1-144) + VKOR + GFP (UNP residues 146-231)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | UA7 Brodifacoum × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；295 K；32% PEG400, 0.1 M ammonium acetate, 1% 1,2,3-heptanetriol, 0.1 M MES, pH 6.5
|
Resolution 1.99 Å R-free 0.215 |
| 7A7M rsGreen0.7b in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；150 mM KBr
30 % PEG 2000 mme
|
Resolution 1.60 Å R-free 0.198 |
| 7A7N rsGreen0.7b in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT pH 5.0
25 % PEG 1500
|
Resolution 1.20 Å R-free 0.170 |
| 7A7O rsGreen0.7-K206A in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM Ammonium acetate
100 mM Bis-tris pH 5.5
25 % PEG 3350
|
Resolution 1.80 Å R-free 0.241 |
| 7A7P rsGreen0.7-K206A partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000
140 mM Mg(NO3)2
|
Resolution 1.48 Å R-free 0.197 |
| 7A7Q rsGreenF-K206A in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl
100 mM MES pH 6.0
20 % PEG 6000
|
Resolution 2.00 Å R-free 0.218 |
| 7A7R rsGreenF-K206A in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl
100 mM MES pH 6.0
20 % PEG 6000
|
Resolution 2.35 Å R-free 0.243 |
| 7A7S rsGreenF in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MIB pH 4.0
25 % PEG 1500
|
Resolution 1.73 Å R-free 0.235 |
| 7A7T rsGreenF partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MIB pH 4.0
25 % PEG 1500
|
Resolution 1.58 Å R-free 0.197 |
| 7A7U rsGreen1 in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；2.0 M (NH4)2SO4
200 mM NaCl
100 mM Na-cacodylate pH 6.5
|
Resolution 2.15 Å R-free 0.201 |
| 7A7V rsGreen1-K206A partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 5.5
200 mM (NH4)2SO4
16 % PEG 8000
|
Resolution 2.00 Å R-free 0.194 |
| 7A7W rsGreen0.7-F145M partially in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NH4HCO2
20 % PEG 4000
|
Resolution 1.30 Å R-free 0.165 |
| 7A7X rsGreen0.7-F145M in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT pH 8.0
25 % PEG 1500
|
Resolution 1.85 Å R-free 0.207 |
| 7A7Y rsGreen0.7-F145Q in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；200 mM KNO3
20 % PEG 3350
|
Resolution 0.97 Å R-free 0.142 |
| 7A7Z rsGreen0.7-K206A-E222G in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM (Li)2SO4
100 mM Tris pH 8.5
40 % PEG 400
|
Resolution 1.10 Å R-free 0.142 |
| 7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000
500 mM Mg(NO3)
50 mM HEPES pH 7.5
|
Resolution 2.05 Å R-free 0.234 |
| 7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000
500 mM Mg(NO3)
50 mM HEPES pH 7.5
|
Resolution 2.05 Å R-free 0.234 |
| 7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000
500 mM Mg(NO3)
50 mM HEPES pH 7.5
|
Resolution 2.05 Å R-free 0.234 |
| 7A80 rsGreen0.7-K206A-E222V in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；26 % PEG 4000
500 mM Mg(NO3)
50 mM HEPES pH 7.5
|
Resolution 2.05 Å R-free 0.234 |
| 7A81 rsGreen0.7-K206A-F145A partially in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl
100 mM MES pH 6.0
20 % PEG 6000
4% 2,2,2-trifluoroethanol
|
Resolution 1.70 Å R-free 0.248 |
| 7A82 rsGreen0.7-K206A-F145A partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl
100 mM MES pH 6.0
20 % PEG 6000
200 mM NDSB-211
|
Resolution 1.90 Å R-free 0.250 |
| 7A83 rsGreen0.7-K206A-F145H in the green-on-state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；125 mM MES pH 6.0
9 % PEG 4000
|
Resolution 1.73 Å R-free 0.192 |
| 7A84 rsGreen0.7-K206A-F145H partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0
14 % PEG 400
300 mM NGSB-195
|
Resolution 2.10 Å R-free 0.269 |
| 7A85 rsGreen0.7-K206A-F145L in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
12 % PEG 20 000
10 mM NaBr
|
Resolution 1.52 Å R-free 0.237 |
| 7A86 rsGreen0.7-K206A-F145L partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
12 % PEG 20 000
4 % Pentaerythritol ethoxylate
|
Resolution 1.90 Å R-free 0.210 |
| 7A86 rsGreen0.7-K206A-F145L partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
12 % PEG 20 000
4 % Pentaerythritol ethoxylate
|
Resolution 1.90 Å R-free 0.210 |
| 7A87 rsGreen0.7-K206A-F145M in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 1500
|
Resolution 1.75 Å R-free 0.228 |
| 7A88 rsGreen0.7-K206A-F145M in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MMT
25 % PEG 1500
|
Resolution 1.95 Å R-free 0.226 |
| 7A89 rsGreen0.7-K206A-F145Q in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000
140 mM Mg(NO3)2
|
Resolution 2.50 Å R-free 0.234 |
| 7A89 rsGreen0.7-K206A-F145Q in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；22 % PEG 4000
140 mM Mg(NO3)2
|
Resolution 2.50 Å R-free 0.234 |
| 7A8A rsGreen0.7-K206A-F145Q partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM NaCl
100 mM MES pH 6.0
20 % PEG 6000
|
Resolution 1.93 Å R-free 0.254 |
| 7A8B rsGreen0.7-K206A-F145S partially in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0
14 % PEG 4000
|
Resolution 1.55 Å R-free 0.232 |
| 7A8C rsGreen0.7-K206A-F145S in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
25 % PEG 4000
400 mM D-galactose
|
Resolution 2.13 Å R-free 0.253 |
| 7A8D rsGreen0.7-K206A-F165W partially in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；291 K；200 mM NH4F
20 % PEG 3350
|
Resolution 1.65 Å R-free 0.219 |
| 7A8E rsGreen0.7-K206A-F165W partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5
25 % PEG 4000
|
Resolution 2.20 Å R-free 0.261 |
| 7A8F rsGreen0.7-K206A-F165L in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5
25 % PEG 4000
|
Resolution 2.27 Å R-free 0.257 |
| 7A8G rsGreen0.7-K206A-H148G in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM TMAO
100mM Tris pH 8.5
20 % Peg 2000 mme
|
Resolution 2.00 Å R-free 0.217 |
| 7A8G rsGreen0.7-K206A-H148G in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM TMAO
100mM Tris pH 8.5
20 % Peg 2000 mme
|
Resolution 2.00 Å R-free 0.217 |
| 7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 1.70 Å R-free 0.209 |
| 7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 1.70 Å R-free 0.209 |
| 7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 1.70 Å R-free 0.209 |
| 7A8H rsGreen0.7-K206A-H148S in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 1.70 Å R-free 0.209 |
| 7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 2.00 Å R-free 0.228 |
| 7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 2.00 Å R-free 0.228 |
| 7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 2.00 Å R-free 0.228 |
| 7A8I rsGreen0.7-K206A-H148S partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM Bis-tris pH 6.5
20 % PEG 5000 mme
|
Resolution 2.00 Å R-free 0.228 |
| 7A8J rsGreen0.7-K206A-H148V in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
25 % PEG 550 mme
10 mM ZnSO4
|
Resolution 1.85 Å R-free 0.232 |
| 7A8K rsGreen0.7-K206A-H148V partially in the green-off state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；50 mM HEPES pH 7.5
12 % PEG 4000
1% tryptone
|
Resolution 2.25 Å R-free 0.263 |
| 7A8L rsGreen0.7-K206A-N205C in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.5
25 % PEG 3000
|
Resolution 1.75 Å R-free 0.225 |
| 7A8M rsGreen0.7-K206A-N205G in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM MES pH 6.0
20 % PEG 2000 mme
10 mM BaCl2
|
Resolution 1.60 Å R-free 0.245 |
| 7A8N rsGreen0.7-K206A-N205L in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；100 mM HEPES pH 7.5
25 % PEG 8000
|
Resolution 2.10 Å R-free 0.258 |
| 7A8O rsGreen0.7-K206A-N205S in the green-on state Deposited 2020-08-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；200 mM Maic acid pH 7.0
20 % PEG 3350
|
Resolution 1.60 Å R-free 0.201 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain F
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain H
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain J
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain M
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7BWN Crystal Structure of a Designed Protein Heterocatenane Deposited 2020-04-15 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain O
1–238（238 aa）
|
Mutation：R2S,S30R,A72S,Q80R,A206V,M340E,L344K | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298.15 K；Bis-Tris, (NH4)2SO4
|
Resolution 2.40 Å R-free 0.239 |
| 7E53 Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution Deposited 2021-02-16 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–232（230 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；30% (w/v) Polyethylene glycol 400, 100mM CAPS/ Sodium hydroxide, pH 10.5
|
Resolution 2.21 Å R-free 0.258 |
| 8DHY N-terminal fragment of MsbA fused to GFP in complex with copper(II) Deposited 2022-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–239（237 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；70% Tacsimate pH 7.0
|
Resolution 2.15 Å R-free 0.228 |
| 8GW6 AtSLAC1 6D mutant in closed state Deposited 2022-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–240（240 aa）
Chain B
1–240（240 aa）
Chain C
1–240（240 aa）
|
Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D | CL CHLORIDE ION × 3 Y01 CHOLESTEROL HEMISUCCINATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8GW7 AtSLAC1 6D mutant in open state Deposited 2022-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–240（240 aa）
Chain B
1–240（240 aa）
Chain C
1–240（240 aa）
|
Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D Mutation：T62D,S65D,S107D,S124D,S146D,T152D | CL CHLORIDE ION × 6 Y01 CHOLESTEROL HEMISUCCINATE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8J6M SIDT1 protein Deposited 2023-04-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–236（234 aa）
Chain B
3–236（234 aa）
|
Not recorded | CLR CHOLESTEROL × 8 ZN ZINC ION × 2 NA SODIUM ION × 1 OLA OLEIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.77 Å |
| 8J6O transport T2 Deposited 2023-04-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
3–236（234 aa）
Chain B
3–236（234 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 ZN ZINC ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å |
| 8J7M ion channel Deposited 2023-04-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
3–235（233 aa）
Chain B
3–235（233 aa）
Chain C
3–235（233 aa）
Chain D
3–235（233 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8RL9 RECQL5:sfGFP hetero dimer assembled by Di-Gluebody Deposited 2024-01-02 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–238（238 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.22 Å |
| 8RLB RECQL5:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement Deposited 2024-01-02 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
1–238（238 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.99 Å |
| 8RLC SPNS2:sfGFP hetero dimer assembled by Di-Gluebody Deposited 2024-01-02 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain B
1–238（238 aa）
|
Not recorded | LMT DODECYL-BETA-D-MALTOSIDE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8RLE SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement Deposited 2024-01-02 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
1–238（238 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.75 Å |
| 8UBG DpHF19 filament Deposited 2023-09-22 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 20 PDB declaration: 20-meric |
Chain A
3–238（236 aa）
Chain B
3–238（236 aa）
Chain C
3–238（236 aa）
Chain D
3–238（236 aa）
Chain E
3–238（236 aa）
Chain F
3–238（236 aa）
Chain G
3–238（236 aa）
Chain H
3–238（236 aa）
Chain I
3–238（236 aa）
Chain J
3–238（236 aa）
Chain K
3–238（236 aa）
Chain L
3–238（236 aa）
Chain M
3–238（236 aa）
Chain N
3–238（236 aa）
Chain O
3–238（236 aa）
Chain P
3–238（236 aa）
Chain Q
3–238（236 aa）
Chain R
3–238（236 aa）
Chain S
3–238（236 aa）
Chain T
3–238（236 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8XAL Cryo-EM structure of SARS-CoV-2 S-BQ.1 in complex with ACE2 Deposited 2023-12-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain I
3–238（236 aa）
Chain J
3–238（236 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 31 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
|
Resolution 3.20 Å |
| 9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
|
Resolution 3.20 Å R-free 0.238 |
| 9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
|
Resolution 3.20 Å R-free 0.238 |
| 9BCF Chimeric protein of crocodile allergen Cro p 1.0101 and GFP Deposited 2024-04-09 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300
|
Resolution 3.20 Å R-free 0.238 |
| 9C74 superfolder Green Fluorescent Protein with meta-nitro-tyrosine incorporated at position 66 Deposited 2024-06-10 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–238（238 aa）
|
Mutation：Y66mNO2Y Non-standard monomer：Yes (specific site not provided by mmCIF) | NA SODIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；1% tryptone, 0.001 M NaN3, 0.05 M Hepes pH 7.0, 20% w/v PEG350
|
Resolution 1.51 Å R-free 0.220 |
| 9IRV MultiBody Refinement of dimeric DARPin and its bound GFP on a symmetric scaffold Deposited 2024-07-16 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
1–231（231 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.47 Å |
| 9J48 GFP bound to 24-mer DARPin-apoferritin model 6c Deposited 2024-08-09 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric |
Chain a
1–231（231 aa）
Chain b
1–231（231 aa）
Chain c
1–231（231 aa）
Chain d
1–231（231 aa）
Chain e
1–231（231 aa）
Chain f
1–231（231 aa）
Chain g
1–231（231 aa）
Chain h
1–231（231 aa）
Chain i
1–231（231 aa）
Chain j
1–231（231 aa）
Chain k
1–231（231 aa）
Chain l
1–231（231 aa）
Chain m
1–231（231 aa）
Chain n
1–231（231 aa）
Chain o
1–231（231 aa）
Chain p
1–231（231 aa）
Chain q
1–231（231 aa）
Chain r
1–231（231 aa）
Chain s
1–231（231 aa）
Chain t
1–231（231 aa）
Chain v
1–231（231 aa）
Chain w
1–231（231 aa）
Chain x
1–231（231 aa）
Chain y
1–231（231 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9N6A GP23 C-termimal receptor binding domain from TM4 phage Deposited 2025-02-05 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
1–238（238 aa）
|
Not recorded | SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；289 K；0.2 M Lithium Sulfate
0.1 M MES: NaOH, pH 6
20 % (v/v) 1,4-Butanediol
|
Resolution 2.91 Å R-free 0.280 |
| 9V85 Superfolder GFP fused gp38 receptor binding domain of bacteriophage PP01 Deposited 2025-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；10% (w/v) PEG8000, 0.1M Na-HEPES-MOPS, pH7.5, 3mM CaCl2, 3mM MgCl2, 20 % (v/v) ethylene glycol
|
Resolution 2.10 Å R-free 0.208 |
| 9V85 Superfolder GFP fused gp38 receptor binding domain of bacteriophage PP01 Deposited 2025-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
3–238（236 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293.15 K；10% (w/v) PEG8000, 0.1M Na-HEPES-MOPS, pH7.5, 3mM CaCl2, 3mM MgCl2, 20 % (v/v) ethylene glycol
|
Resolution 2.10 Å R-free 0.208 |
| 9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
3–239（237 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.40 Å R-free 0.331 |
| 9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
3–239（237 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.40 Å R-free 0.331 |
| 9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
3–239（237 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.40 Å R-free 0.331 |
| 9ZG5 Structure of superfolder GFP bound to nanobody 15 Deposited 2025-12-02 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain G
3–239（237 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.40 Å R-free 0.331 |
91 other PDB entries and 132 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | A0A059PIQ0_AEQVI |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 2–237； UniProt 1–238 Author chain B； PDBConstruct 2–237； UniProt 1–238 Author chain C； PDBConstruct 2–237； UniProt 1–238 Author chain D； PDBConstruct 2–237； UniProt 1–238 Author chain E； PDBConstruct 2–237； UniProt 1–238 Author chain F； PDBConstruct 2–237； UniProt 1–238 |
