5f9h

Crystal structure of RIG-I helicase-RD in complex with 24-mer 5' triphosphate hairpin RNA

Method: X-RAY DIFFRACTION

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Probable ATP-dependent RNA helicase DDX58

Homo sapiens

当前结构中的状态

Assembly	物理组成	蛋白状态	分子拷贝数	共同组分	数据核对
1	蛋白–RNA	同源多聚体	蛋白 6 RNA 6	;RNA (5'-R(PAPAPUPAPUPAPAPUPAPGPUPGPAPUPAPUPUPAPUPAPUPUP*C)-3') ; × 6 ZINC ION × 6 MAGNESIUM ION × 8 GUANOSINE-5'-TRIPHOSPHATE × 6	与全部聚合物一致

数据库中的同蛋白其他状态

查看构建体与数据证据

UniProt名称	DDX58_HUMAN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 2–695； UniProt 232–925 作者链 C； PDB构建体 2–695； UniProt 232–925 作者链 E； PDB构建体 2–695； UniProt 232–925 作者链 G； PDB构建体 2–695； UniProt 232–925 作者链 I； PDB构建体 2–695； UniProt 232–925 作者链 K； PDB构建体 2–695； UniProt 232–925

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	5f9h
沉积日期 deposition_date	2015-12-09
结构标题 title	Crystal structure of RIG-I helicase-RD in complex with 24-mer 5' triphosphate hairpin RNA
关键词 keywords	Complex, RIG-I, capped RNA, self versus non-self, innate immunity, hydrolase-rna complex; hydrolase/rna
实验方法 method	X-RAY DIFFRACTION

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3. Official assembly/model 理论 SAXS Official SAXS Profiles

页面只读取最新 assembly 计算结果。每一条曲线都对应明确的 PDB、official biological assembly 和 coordinate model。

5f9h__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

下载该曲线 .dat

5f9h__assembly_1__model_1 | I(q)

100 个绘图点；横轴和纵轴均采用对数尺度。

5f9h__assembly_1__model_1 | P(r) · Pending

P(r) 将按相同 assembly/model 分别计算和展示。

Rg（Guinier）	59.41 Å
Rg（电子密度）	59.00 Å
总 Rg	59.11 Å
原子数	33602
残基数	4003
排除体积	590390 Å³
最大 q	0.500 Å⁻¹

Assembly	Model	结构单元	聚集说明	状态	CRYSOL	操作
1	1	`5f9h__assembly_1__model_1`	dodecameric （12）	成功	4.1.3-1-20251215 (887e7ef)	查看下载

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合物信息 Entities & Polymers (5)

实体 1 Entity 1 polymer

描述 description	Probable ATP-dependent RNA helicase DDX58
分子量 formula_weight	79594.7 kDa
EC 编号 ec_number	3.6.4.13
来源方法 src_method	man
拷贝数 entity_count	6

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,C,E,G,I,K
原子数 atom_count	33602

SVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK

来源 Source

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

实体 2 Entity 2 polymer

描述 description	;RNA (5'-R(PAPAPUPAPUPAPAPUPAPGPUPGPAPUPAPUPUPAPUPAPUPUP*C)-3') ;
分子量 formula_weight	7304.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

聚合物 Polymer

聚合物类型 type	polyribonucleotide
链编号 strand_id	B,D,F,H,J,L

AAUAUAAUAGUGAUAUUAUAUUC

实体 3 Entity 3 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

实体 4 Entity 4 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	8

实体 5 Entity 5 non-polymer

描述 description	GUANOSINE-5'-TRIPHOSPHATE
分子量 formula_weight	523.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	6

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6. 折叠分类 (SCOP + CATH) 24 domains

CATH v4.4 (24 domains)

结构域编号 domain_id	5f9hA01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hA03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hA04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

结构域编号 domain_id	5f9hC01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hC02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hC03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hC04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

结构域编号 domain_id	5f9hE01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hE02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hE03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hE04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

结构域编号 domain_id	5f9hG01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hG02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hG03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hG04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

结构域编号 domain_id	5f9hI01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hI02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hI03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hI04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

结构域编号 domain_id	5f9hK01
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hK02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

结构域编号 domain_id	5f9hK03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	1320 — phosphoenolpyruvate carboxylase, domain 3
同源超家族 H-superfamily homologous superfamily	30 —

结构域编号 domain_id	5f9hK04
类 Class class	2 — Mainly Beta
架构 Architecture architecture	170 — Beta Complex
拓扑 Topology topology	150 — Metal Binding Protein, Guanine Nucleotide Exchange Factor; Chain A
同源超家族 H-superfamily homologous superfamily	30 — RIG-I-like receptor, C-terminal regulatory domain

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晶胞边长 a length_a	111.180 Å
晶胞边长 b length_b	174.810 Å
晶胞边长 c length_c	309.280 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	24

空间群 space_group	P 21 21 21
空间群编号 int_tables_number	19
晶系 crystal_system	—

分辨率 resolution	3.100 Å
最低分辨率 resolution_low	49.442 Å
R 因子 r_factor	0.2160
R_work r_work	0.2135
R_free r_free	0.2656

Crystal structure of RIG-I helicase-RD in complex with 24-mer 5' triphosphate hairpin RNA

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Probable ATP-dependent RNA helicase DDX58

当前结构中的状态

数据库中的同蛋白其他状态

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

5f9h__assembly_1__model_1

5f9h__assembly_1__model_1 | I(q)

5f9h__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (5)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

6. 折叠分类 (SCOP + CATH) 24 domains

CATH v4.4 (24 domains)

7. 引用文献 (1)

Crystal structure of RIG-I helicase-RD in complex with 24-mer 5' triphosphate hairpin RNA

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Probable ATP-dependent RNA helicase DDX58

当前结构中的状态

数据库中的同蛋白其他状态

单体

同源二聚体

同源四聚体

其他同源多聚体

异源或核酸复合物

2. 结构基本信息 2. Structure Basics

3. Official assembly/model 理论 SAXS Official SAXS Profiles

5f9h__assembly_1__model_1

5f9h__assembly_1__model_1 | I(q)

5f9h__assembly_1__model_1 | P(r) · Pending

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合物信息 Entities & Polymers (5)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 polymer

聚合物 Polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 non-polymer

实体 5 Entity 5 non-polymer

6. 折叠分类 (SCOP + CATH) 24 domains

CATH v4.4 (24 domains)

7. 引用文献 (1)