Photosystem II protein D1 1
OrganismNot specified
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count | Chain A； UniProt 11–344 | Fragment：UNP RESIDUES 11-344 | Photosystem II CP47 reaction center protein × 1 （Q8DIQ1） Photosystem II CP43 reaction center protein × 1 （Q8DIF8） Photosystem II D2 protein × 1 （Q8CM25） Cytochrome b559 subunit alpha × 1 （Q8DIP0） Cytochrome b559 subunit beta × 1 （Q8DIN9） Photosystem II reaction center protein H × 1 （Q8DJ43） Photosystem II reaction center protein I × 1 （Q8DJZ6） Photosystem II reaction center protein J × 1 （P59087） Photosystem II reaction center protein K × 1 （Q9F1K9） Photosystem II reaction center protein L × 1 （Q8DIN8） Photosystem II manganese-stabilizing polypeptide × 1 （P0A431） Photosystem II reaction center protein T × 1 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 1 （Q9F1L5） Cytochrome c-550 × 1 （P0A386） Photosystem II reaction center protein Ycf12 × 1 （Q8DJI1） Photosystem II reaction center X protein × 1 （Q9F1R6） Photosystem II reaction center protein Z × 1 （Q8DHJ2） OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 UNL UNKNOWN LIGAND × 13 DMS DIMETHYL SULFOXIDE × 67 BCT BICARBONATE ION × 1 CA CALCIUM ION × 2 HTG heptyl 1-thio-beta-D-glucopyranoside × 9 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate | Resolution 2.20 Å R-free 0.226 |
| 2 | Protein heterocomplex Heteromer Protein × 18 PDB declaration: octadecameric（18） Consistent with protein copy count | Chain a； UniProt 11–344 | Fragment：UNP RESIDUES 11-344 | Photosystem II CP47 reaction center protein × 1 （Q8DIQ1） Photosystem II CP43 reaction center protein × 1 （Q8DIF8） Photosystem II D2 protein × 1 （Q8CM25） Cytochrome b559 subunit alpha × 1 （Q8DIP0） Cytochrome b559 subunit beta × 1 （Q8DIN9） Photosystem II reaction center protein H × 1 （Q8DJ43） Photosystem II reaction center protein I × 1 （Q8DJZ6） Photosystem II reaction center protein J × 1 （P59087） Photosystem II reaction center protein K × 1 （Q9F1K9） Photosystem II reaction center protein L × 1 （Q8DIN8） Photosystem II manganese-stabilizing polypeptide × 1 （P0A431） Photosystem II reaction center protein T × 1 （Q8DIQ0） Photosystem II 12 kDa extrinsic protein × 1 （Q9F1L5） Cytochrome c-550 × 1 （P0A386） Photosystem II reaction center protein Ycf12 × 1 （Q8DJI1） Photosystem II reaction center X protein × 1 （Q9F1R6） Photosystem II reaction center protein Z × 1 （Q8DHJ2） OEX CA-MN4-O5 CLUSTER × 1 FE2 FE (II) ION × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 5 UNL UNKNOWN LIGAND × 14 DMS DIMETHYL SULFOXIDE × 66 BCT BICARBONATE ION × 1 CA CALCIUM ION × 3 HTG heptyl 1-thio-beta-D-glucopyranoside × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 | X-RAY DIFFRACTION X-ray crystallization conditions：MICROBATCH；pH 6.1；285 K；PEG1450, sodium chloride, calcium chloride, magnesium sulfate | Resolution 2.20 Å R-free 0.226 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 5H2F | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 11YO Single-conformation model re-refinement of 2F/S3-rich PSII intermediate structure at 2.09 Angstrom resolution Deposited 2026-03-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 38 OEX CA-MN4-O5 CLUSTER × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.243 |
| 1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric |
Chain A
1–344（344 aa）
|
Not recorded | FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
|
Resolution 3.50 Å R-free 0.342 |
| 1S5L Architecture of the photosynthetic oxygen evolving center Deposited 2004-01-21 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric |
Chain a
1–344（344 aa）
|
Not recorded | FE FE (III) ION × 1 BCT BICARBONATE ION × 2 OEC OXYGEN EVOLVING SYSTEM × 1 CLA CHLOROPHYLL A × 36 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMT DODECYL-BETA-D-MALTOSIDE × 1 BCR BETA-CAROTENE × 7 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pseudo-batch hanging drop；pH 7.5；291 K；100mM HEPES, 100mM (NH4)2SO4, PEG 4000, C12E8, trimethyl lead acetate, pH 7.5, pseudo-batch hanging drop, temperature 291K
|
Resolution 3.50 Å R-free 0.342 |
| 1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
1–360（360 aa）
|
Not recorded | CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.20 Å |
| 1W5C Photosystem II from Thermosynechococcus elongatus Deposited 2004-08-06 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 10 PDB declaration: decameric |
Chain G
1–360（360 aa）
|
Not recorded | CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MN MANGANESE (II) ION × 4 FE2 FE (II) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 BCR BETA-CAROTENE × 1 HEC HEME C × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 3.20 Å |
| 3KZI Crystal Structure of Monomeric Form of Cyanobacterial Photosystem II Deposited 2009-12-08 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric |
Chain A
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 LMT DODECYL-BETA-D-MALTOSIDE × 7 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 BCT BICARBONATE ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
batch；pH 7；291 K；PEG 400, PIPES, MES, CaCl2, pH 7, batch, temperature 291.0K
|
Resolution 3.60 Å R-free 0.308 |
| 4FBY fs X-ray diffraction of Photosystem II Deposited 2012-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain G
1–344（344 aa）
|
Not recorded | CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEC OXYGEN EVOLVING SYSTEM × 2 FE2 FE (II) ION × 2 BCR BETA-CAROTENE × 24 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7；298 K；4% PEG 2000, 5 mM CaCl2, 100 mM PIPES, pH 7.0, batch, temperature 298K
|
Resolution 6.56 Å R-free 0.385 |
| 4IXQ RT fs X-ray diffraction of Photosystem II, dark state Deposited 2013-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–360（360 aa）
Chain a
1–360（360 aa）
|
Not recorded | FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 5.70 Å R-free 0.314 |
| 4IXR RT fs X-ray diffraction of Photosystem II, first illuminated state Deposited 2013-01-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–360（360 aa）
Chain a
1–360（360 aa）
|
Not recorded | FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 LMT DODECYL-BETA-D-MALTOSIDE × 14 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 5.90 Å R-free 0.313 |
| 4PBU Serial Time-resolved crystallography of Photosystem II using a femtosecond X-ray laser The S1 state Deposited 2014-04-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 7 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
|
Resolution 5.00 Å R-free 0.262 |
| 4PJ0 Structure of T.elongatus Photosystem II, rows of dimers crystal packing Deposited 2014-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE FE (III) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SO4 SULFATE ION × 10 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 29 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；pH 7.5；293 K；Batch crystallisation: 2 mM Chl a equivalent of PSIIcc mixed 1:1 with: 0.1 M TRIS (pH 7.5), 0.1 M (NH4)2SO4, 15 - 18 % PEG 5000 MME, post-crystallisation dehydration
|
Resolution 2.44 Å R-free 0.256 |
| 4RVY Serial Time resolved crystallography of Photosystem II using a femtosecond X-ray laser. The S state after two flashes (S3) Deposited 2014-11-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
Free interface diffusion；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000 , Free interface diffusion, temperature 283K, temperature 283.0K
|
Resolution 5.50 Å R-free 0.291 |
| 4TNH RT XFEL structure of Photosystem II in the dark state at 4.9 A resolution Deposited 2014-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 4.90 Å R-free 0.292 |
| 4TNI RT XFEL structure of Photosystem II 500 ms after the third illumination at 4.6 A resolution Deposited 2014-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 2 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 4.60 Å R-free 0.284 |
| 4TNJ RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 4.5 A resolution Deposited 2014-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 4.50 Å R-free 0.284 |
| 4TNK RT XFEL structure of Photosystem II 250 microsec after the third illumination at 5.2 A resolution Deposited 2014-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 6 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 CL CHLORIDE ION × 2 OEX CA-MN4-O5 CLUSTER × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7；298 K；4% PEG2000, 5 mM calcium chloride, 100 mM PIPES, pH 7.0, BATCH, temperature 298K
|
Resolution 5.20 Å R-free 0.289 |
| 4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain AA
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
|
Resolution 2.90 Å R-free 0.292 |
| 4V62 Crystal Structure of cyanobacterial Photosystem II Deposited 2008-01-17 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain BA
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | FE2 FE (II) ION × 1 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 3 OEC OXYGEN EVOLVING SYSTEM × 1 BCR BETA-CAROTENE × 11 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 7 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 6 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 CL CHLORIDE ION × 1 LMT DODECYL-BETA-D-MALTOSIDE × 7 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 CA CALCIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
batch；pH 7；298 K；PEG2000, PIPES, magnesium chloride, pH7.0, batch, temperature 298K
|
Resolution 2.90 Å R-free 0.292 |
| 4V82 Crystal structure of cyanobacterial Photosystem II in complex with terbutryn Deposited 2010-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain AA
1–344（344 aa）
Chain BA
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 BCT BICARBONATE ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 MST 2-T-BUTYLAMINO-4-ETHYLAMINO-6-METHYLTHIO-S-TRIAZINE × 2 OEC OXYGEN EVOLVING SYSTEM × 2 BCR BETA-CAROTENE × 24 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 14 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 22 LMT DODECYL-BETA-D-MALTOSIDE × 16 DMS DIMETHYL SULFOXIDE × 8 PHO PHEOPHYTIN A × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 CA CALCIUM ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
batch；pH 7；298 K；Pipes, CaCl2, DMSO, PEG 2000, pH 7.0, batch, temperature 298K
|
Resolution 3.20 Å R-free 0.299 |
| 5E79 Macromolecular diffractive imaging using imperfect crystals Deposited 2015-10-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 CA CALCIUM ION × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIQUID DIFFUSION；283.18 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
|
Resolution 3.50 Å R-free 0.331 |
| 5E7C Macromolecular diffractive imaging using imperfect crystals - Bragg data Deposited 2015-10-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 CL CHLORIDE ION × 6 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 FE2 FE (II) ION × 2 CA CALCIUM ION × 6 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 MG MAGNESIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIQUID DIFFUSION；pH 7；283 K；100 mM Pipes pH 7.0, 5 mM CaCl2, 10 mM tocopherol, and 10-17% PEG 2000
|
Resolution 4.50 Å R-free 0.275 |
| 5KAF RT XFEL structure of Photosystem II in the dark state at 3.0 A resolution Deposited 2016-06-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 UNL UNKNOWN LIGAND × 17 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol.
|
Resolution 3.00 Å R-free 0.303 |
| 5KAI NH3-bound RT XFEL structure of Photosystem II 500 ms after the 2nd illumination (2F) at 2.8 A resolution Deposited 2016-06-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 UNL UNKNOWN LIGAND × 23 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 7.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
|
Resolution 2.80 Å R-free 0.300 |
| 5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain A
1–344（344 aa）
|
Not recorded | CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 19 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
|
Resolution 2.20 Å R-free 0.260 |
| 5MX2 Photosystem II depleted of the Mn4CaO5 cluster at 2.55 A resolution Deposited 2017-01-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 20 PDB declaration: eicosameric |
Chain a
1–344（344 aa）
|
Not recorded | CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 11 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 4 LFA EICOSANE × 20 FE FE (III) ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 6 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 4 BCT BICARBONATE ION × 1 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 5 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 7.5；291 K；equal volumes of buffer: 0.1 M Tris-HCl pH 7.5, 0.1 M (NH4)2SO4, 14-18 % PEG 5000 MME and protein: 2 mM chlorophyll equivalent of PSII in C12E8 detergent, post crystallisation treatment
|
Resolution 2.20 Å R-free 0.260 |
| 5TIS Room temperature XFEL structure of the native, doubly-illuminated photosystem II complex Deposited 2016-10-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Fragment：UNP residues 1-344
Chain a
1–344（344 aa）
Fragment：UNP residues 1-344
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 UNL UNKNOWN LIGAND × 33 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；293 K；Crystal growth was as described in Kern et al. 2005, Biochim. Biophys. Acta-Bioenergetics and Ibrahim et al. 2015, Struct. Dyn. 2 (4), 041705, substituting C12E8 for beta-DM and Betaine for glycerol
|
Resolution 2.25 Å R-free 0.231 |
| 5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric |
Chain A
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 27 LMT DODECYL-BETA-D-MALTOSIDE × 9 DMS DIMETHYL SULFOXIDE × 48 CA CALCIUM ION × 3 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 12 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 2 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
|
Resolution 2.10 Å R-free 0.197 |
| 5ZZN Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus Deposited 2018-06-03 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 19 PDB declaration: nonadecameric |
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 1 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 35 PHO PHEOPHYTIN A × 2 BCR BETA-CAROTENE × 10 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 5 UNL UNKNOWN LIGAND × 25 LMT DODECYL-BETA-D-MALTOSIDE × 10 DMS DIMETHYL SULFOXIDE × 47 CA CALCIUM ION × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 6 HTG heptyl 1-thio-beta-D-glucopyranoside × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 5 GOL GLYCEROL × 3 FE2 FE (II) ION × 1 BCT BICARBONATE ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 RRX (3R)-beta,beta-caroten-3-ol × 1 MG MAGNESIUM ION × 2 HEC HEME C × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MICROBATCH；pH 6；285 K；5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K
|
Resolution 2.10 Å R-free 0.197 |
| 6DHE RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.05 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 8CT (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta,beta-carotene × 12 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 10 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.05 Å R-free 0.241 |
| 6DHF RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.08 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.08 Å R-free 0.247 |
| 6DHG RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.5 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.50 Å R-free 0.246 |
| 6DHH RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.2 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.20 Å R-free 0.264 |
| 6DHO RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.07 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 UNL UNKNOWN LIGAND × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.07 Å R-free 0.247 |
| 6DHP RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.04 Angstrom resolution Deposited 2018-05-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 UNL UNKNOWN LIGAND × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.04 Å R-free 0.248 |
| 6W1O RT XFEL structure of the dark-stable state of Photosystem II (0F, S1-rich) at 2.08 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.08 Å R-free 0.240 |
| 6W1P RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
11–344（334 aa）
Chain a
11–344（334 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.26 Å R-free 0.241 |
| 6W1Q RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 HEC HEME C × 4 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.27 Å R-free 0.249 |
| 6W1R RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 BCT BICARBONATE ION × 2 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
|
Resolution 2.23 Å R-free 0.235 |
| 6W1T RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
|
Resolution 2.01 Å R-free 0.228 |
| 6W1U RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 STE STEARIC ACID × 35 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.239 |
| 6W1V RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2020-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 STE STEARIC ACID × 38 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 BCT BICARBONATE ION × 2 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 PHO PHEOPHYTIN A × 4 HEC HEME C × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.241 |
| 7NHO Structure of PSII-M Deposited 2021-02-11 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric |
Chain A
1–360（360 aa）
|
Not recorded | FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 2 BCT BICARBONATE ION × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.66 Å |
| 7NHP Structure of PSII-I (PSII with Psb27, Psb28, and Psb34) Deposited 2021-02-11 | Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 18 PDB declaration: octadecameric |
Chain A
1–360（360 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 FE FE (III) ION × 1 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.72 Å |
| 7NHQ Structure of PSII-I prime (PSII with Psb28, and Psb34) Deposited 2021-02-11 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 17 PDB declaration: heptadecameric |
Chain A
1–360（360 aa）
|
Not recorded | FE FE (III) ION × 1 MN MANGANESE (II) ION × 1 CL CHLORIDE ION × 1 PHO PHEOPHYTIN A × 2 CLA CHLOROPHYLL A × 35 BCR BETA-CAROTENE × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 1 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 7 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 2.68 Å |
| 7RF1 RT XFEL structure of Photosystem II averaged across all S-states at 1.89 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 55 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 16 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 1.89 Å R-free 0.214 |
| 7RF2 RT XFEL structure of dark-stable state of Photosystem II (0F, S1 rich) at 2.08 Angstrom Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 33 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.08 Å R-free 0.238 |
| 7RF3 RT XFEL structure of the one-flash state of Photosystem II (1F, S2-rich) at 2.26 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
|
Resolution 2.26 Å R-free 0.238 |
| 7RF4 RT XFEL structure of Photosystem II 50 microseconds after the second illumination at 2.27 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEX CA-MN4-O5 CLUSTER × 2 BCR BETA-CAROTENE × 22 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES, pH 6.5, 0.1 M ammonium chloride, 35% w/v PEG5000
|
Resolution 2.27 Å R-free 0.244 |
| 7RF5 RT XFEL structure of Photosystem II 150 microseconds after the second illumination at 2.23 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.23 Å R-free 0.233 |
| 7RF6 RT XFEL structure of Photosystem II 250 microseconds after the second illumination at 2.01 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 STE STEARIC ACID × 35 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.01 Å R-free 0.227 |
| 7RF7 RT XFEL structure of Photosystem II 400 microseconds after the second illumination at 2.09 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 35 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.239 |
| 7RF8 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2021-07-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 CL CHLORIDE ION × 4 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.239 |
| 8EZ5 RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.09 Angstrom resolution Deposited 2022-10-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.234 |
| 8F4C RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.00 Å R-free 0.224 |
| 8F4D RT XFEL structure of Photosystem II 50 microseconds after the third illumination at 2.15 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.15 Å R-free 0.243 |
| 8F4E RT XFEL structure of Photosystem II 250 microseconds after the third illumination at 2.09 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.09 Å R-free 0.233 |
| 8F4F RT XFEL structure of Photosystem II 500 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 PHO PHEOPHYTIN A × 4 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.03 Å R-free 0.248 |
| 8F4G RT XFEL structure of Photosystem II 730 microseconds after the third illumination at 2.03 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.03 Å R-free 0.223 |
| 8F4H RT XFEL structure of Photosystem II 1200 microseconds after the third illumination at 2.10 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEY CA-MN4-O6 CLUSTER × 2 OEX CA-MN4-O5 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 BCT BICARBONATE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.10 Å R-free 0.232 |
| 8F4I RT XFEL structure of Photosystem II 2000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.00 Å R-free 0.232 |
| 8F4J RT XFEL structure of Photosystem II 4000 microseconds after the third illumination at 2.00 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.00 Å R-free 0.226 |
| 8F4K RT XFEL structure of the three-flash state of Photosystem II (3F, S0-rich) at 2.16 Angstrom resolution Deposited 2022-11-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 40 PDB declaration: 40-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | OEX CA-MN4-O5 CLUSTER × 2 OEY CA-MN4-O6 CLUSTER × 2 FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 STE STEARIC ACID × 31 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 13 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；pH 6.5；298 K；0.1 M MES pH 6.5, 0.1 M NH4Cl, 35% (w/v) PEG 5000
|
Resolution 2.16 Å R-free 0.241 |
| 9EVX cryoEM structure of Photosystem II averaged across S2-S3 states at 1.71 Angstrom resolution Deposited 2024-04-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
1–344（344 aa）
Chain a
1–344（344 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 BCR BETA-CAROTENE × 22 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 STE STEARIC ACID × 42 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 14 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 OEX CA-MN4-O5 CLUSTER × 2 PHO PHEOPHYTIN A × 4 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 LMT DODECYL-BETA-D-MALTOSIDE × 14 BCT BICARBONATE ION × 2 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 HEC HEME C × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5；100mM MES pH6.5, 5mM CaCl2, 5% glycerol, 0.03% DDM
cryo-EM vitrification conditions
Cryogen ETHANE；The sample-on-grid was illuminated with one and two flashes of light
|
Resolution 1.71 Å |
| 9G6F Inactive PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 32 PDB declaration: 32-meric |
Chain A
1–360（360 aa）
Chain a
1–360（360 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | FE2 FE (II) ION × 2 CL CHLORIDE ION × 2 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 LFA EICOSANE × 33 PLM PALMITIC ACID × 24 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 9 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 8 LMT DODECYL-BETA-D-MALTOSIDE × 13 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 9 HEM PROTOPORPHYRIN IX CONTAINING FE × 2 RRX (3R)-beta,beta-caroten-3-ol × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9G6G Semi-active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 35 PDB declaration: 35-meric |
Chain A
1–360（360 aa）
Chain a
1–360（360 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | FE2 FE (II) ION × 2 CL CHLORIDE ION × 3 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 9 LFA EICOSANE × 30 OEX CA-MN4-O5 CLUSTER × 1 LMT DODECYL-BETA-D-MALTOSIDE × 17 BCT BICARBONATE ION × 2 PLM PALMITIC ACID × 29 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 GOL GLYCEROL × 1 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 3 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
| 9G6H active PSII dimer from native Peak4 PSII dimers Deposited 2024-07-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 38 PDB declaration: 38-meric |
Chain A
1–360（360 aa）
Chain a
1–360（360 aa）
|
Not recorded | FE2 FE (II) ION × 2 CL CHLORIDE ION × 4 CLA CHLOROPHYLL A × 70 PHO PHEOPHYTIN A × 4 BCR BETA-CAROTENE × 20 PL9 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE × 4 SQD 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 PLM PALMITIC ACID × 35 LFA EICOSANE × 24 OEX CA-MN4-O5 CLUSTER × 2 BCT BICARBONATE ION × 2 LHG 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 GOL GLYCEROL × 2 LMT DODECYL-BETA-D-MALTOSIDE × 17 DGD DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 10 LMG 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 10 HEM PROTOPORPHYRIN IX CONTAINING FE × 4 RRX (3R)-beta,beta-caroten-3-ol × 2 CA CALCIUM ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.5；20 mM MES pH 6.5, 2.5 mM MgCl2, 2.5 mM CaCl2, 7 mM MgSO4, 0.03% DDM, 0.05% CHAPS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.20 Å |
61 other PDB entries and 66 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | PSBA1_THEEB |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–334； UniProt 11–344 Author chain a； PDBConstruct 1–334； UniProt 11–344 |