SAXSdb — 5nt2

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 1

Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 8	E3 ubiquitin/ISG15 ligase TRIM25 × 4 （Q14258）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	NS1_I34A1
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain C； PDBConstruct 4–233； UniProt 1–230 Author chain D； PDBConstruct 4–233； UniProt 1–230 Author chain E； PDBConstruct 4–233； UniProt 1–230 Author chain F； PDBConstruct 4–233； UniProt 1–230

E3 ubiquitin/ISG15 ligase TRIM25

Homo sapiens

UniProt Q14258

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 8	Non-structural protein 1 × 4 （P03496）	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TRI25_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain A； PDBConstruct 4–193； UniProt 190–379 Author chain I； PDBConstruct 4–193； UniProt 190–379 Author chain N； PDBConstruct 4–193； UniProt 190–379 Author chain V； PDBConstruct 4–193； UniProt 190–379

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5nt2
Deposition date deposition_date	2017-04-27
Structure title title	Complex of influenza A NS1 with TRIM25 coiled coil domain
Keywords keywords	Viral protein/host protein/E3 ligase/TRIM protein, ligase; LIGASE
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5nt2__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5nt2__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5nt2__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	52.96 Å
Rg (electron density)	54.23 Å
Total Rg	53.86 Å
Atom count	10516
Residues	1323
Excluded volume	188130 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5nt2__assembly_1__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Description description	Non-structural protein 1
Molecular weight formula_weight	25867.6 kDa
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,C,D,E
Atom count atom_count	10516

GPGMDPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRADQASLRGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSREWSMLIPKQKVAGPLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLPGHTAEDVKNAVGVLIGGLEANDNTVRVSETLQRFAWRSSNENGRPPLTPKQKREMAGTIRSEV

Source Source

Source organism organism	Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
NCBI taxonomy ID ncbi_taxonomy_id	211044
Host organism host_organism	Escherichia coli

Entity 2 Entity 2 polymer

Description description	E3 ubiquitin/ISG15 ligase TRIM25
Molecular weight formula_weight	22011.9 kDa
EC 编号 ec_number	6.3.2.-,2.3.2.27
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I,A,V,N

GPGSLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQELTPSSGDPGEHDPASTHKSTRP

Source Source

Source organism organism	Homo sapiens
Common name common_name	Human
NCBI taxonomy ID ncbi_taxonomy_id	9606
Host organism host_organism	Escherichia coli

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Unit-cell length a length_a	73.257 Å
Unit-cell length b length_b	76.296 Å
Unit-cell length c length_c	92.072 Å
Unit-cell angle α angle_alpha	100.04 °
Unit-cell angle β angle_beta	93.71 °
Unit-cell angle γ angle_gamma	111.14 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.259 Å
Low-resolution limit resolution_low	61.684 Å
R factor r_factor	0.2721
R_work r_work	0.2702
R_free r_free	0.3088

Complex of influenza A NS1 with TRIM25 coiled coil domain

1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 1

State in the Current Structure

Other States of the Same Protein in the Database

E3 ubiquitin/ISG15 ligase TRIM25

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5nt2__assembly_1__model_1

5nt2__assembly_1__model_1 | I(q)

5nt2__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

7. Citations (1)

Complex of influenza A NS1 with TRIM25 coiled coil domain

1. Protein Identity and Related Structures Protein Identity & Related Structures

Non-structural protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

E3 ubiquitin/ISG15 ligase TRIM25

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

5nt2__assembly_1__model_1

5nt2__assembly_1__model_1 | I(q)

5nt2__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 polymer

Polymer Polymer

Source Source

7. Citations (1)