5or5

NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor

Escherichia coli (strain K12)

UniProt P0AGB6

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*AP*AP*A)-3') ; × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RPOE_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–64; UniProt 1–64 Author chain A; PDBConstruct 72–92; UniProt 72–92

ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor

Escherichia coli (strain K12)

UniProt Q45585

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 DNA 1 ;DNA (5'-D(*GP*TP*AP*AP*AP*A)-3') ; × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SIGW_BACSU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 65–70; UniProt 64–69

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id5or5
Deposition date deposition_date2017-08-15
Structure title titleNMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA
Keywords keywordspromoter melting, -10 element recognition, ECF sigma factor, TRANSCRIPTION; TRANSCRIPTION
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5or5__assembly_1__model_8

Assembly 1 · Model 8 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5or5__assembly_1__model_8 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5or5__assembly_1__model_8 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)15.91 Å
Rg (electron density)14.89 Å
Total Rg16.09 Å
Atom count1761
Residues100
Excluded volume15661 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5or5__assembly_1__model_1 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 5or5__assembly_1__model_2 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 5or5__assembly_1__model_3 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 5or5__assembly_1__model_4 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 5or5__assembly_1__model_5 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 5or5__assembly_1__model_6 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 5or5__assembly_1__model_7 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 5or5__assembly_1__model_8 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 5or5__assembly_1__model_9 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 5or5__assembly_1__model_10 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 11 5or5__assembly_1__model_11 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 12 5or5__assembly_1__model_12 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
1 13 5or5__assembly_1__model_13 dimeric (2) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id5or5A00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1740 — Rna Polymerase Sigma Factor; Chain: A
Homologous superfamily homologous superfamily10 — RNA polymerase sigma factor, region 2, helix turn helix motif
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7. Citations (2)