5osc

GLIC-GABAAR alpha1 chimera crystallized in complex with pregnenolone sulfate at pH 4.5

Method: X-RAY DIFFRACTION D_max: 123.5 Å Quality: GOOD

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

Mus musculus

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count	Chain A； UniProt 251–339 Chain B； UniProt 251–339 Chain C； UniProt 251–339 Chain D； UniProt 251–339 Chain E； UniProt 251–339	Not recorded	ACT ACETATE ION × 8 CL CHLORIDE ION × 5 A8W Pregnenolone sulfate × 5 Y01 CHOLESTEROL HEMISUCCINATE × 5	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.5；289 K；12-16% PEG 4000, 100 mM NaCl, 100 mM Li2S04, 100 mM sodium acetate pH 4-5	Resolution 3.10 Å R-free 0.246

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence

UniProt name	GBRA2_MOUSE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 194–282； UniProt 251–339 Author chain B； PDBConstruct 194–282； UniProt 251–339 Author chain C； PDBConstruct 194–282； UniProt 251–339 Author chain D； PDBConstruct 194–282； UniProt 251–339 Author chain E； PDBConstruct 194–282； UniProt 251–339

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

Mus musculus

UniProt P62812

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count	Chain A； UniProt 417–455 Chain B； UniProt 417–455 Chain C； UniProt 417–455 Chain D； UniProt 417–455 Chain E； UniProt 417–455	Not recorded	ACT ACETATE ION × 8 CL CHLORIDE ION × 5 A8W Pregnenolone sulfate × 5 Y01 CHOLESTEROL HEMISUCCINATE × 5	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.5；289 K；12-16% PEG 4000, 100 mM NaCl, 100 mM Li2S04, 100 mM sodium acetate pH 4-5	Resolution 3.10 Å R-free 0.246

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 5OSC	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
5OSA GLIC-GABAAR alpha1 chimera crystallized at pH4.6 Deposited 2017-08-17	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 250–338（89 aa） Chain A 417–455（39 aa） Chain B 250–338（89 aa） Chain B 417–455（39 aa） Chain C 250–338（89 aa） Chain C 417–455（39 aa） Chain D 250–338（89 aa） Chain D 417–455（39 aa） Chain E 250–338（89 aa） Chain E 417–455（39 aa）	Not recorded	ACT ACETATE ION × 8 CL CHLORIDE ION × 7 D12 DODECANE × 8 Y01 CHOLESTEROL HEMISUCCINATE × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；289.15 K；100 mM NaCl, 100 mM sodium acetate pH 4.6, 12 % PEG 6000	Resolution 2.75 Å R-free 0.229
5OSB GLIC-GABAAR alpha1 chimera crystallized in complex with THDOC at pH4.5 Deposited 2017-08-17	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 250–338（89 aa） Chain A 417–455（39 aa） Chain B 250–338（89 aa） Chain B 417–455（39 aa） Chain C 250–338（89 aa） Chain C 417–455（39 aa） Chain D 250–338（89 aa） Chain D 417–455（39 aa） Chain E 250–338（89 aa） Chain E 417–455（39 aa）	Not recorded	A8Z Tetrahydrodeoxycorticosterone × 5 ACT ACETATE ION × 5 CL CHLORIDE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；289 K；12-16% PEG4000, 100mM NaCl, 100 mM Li2SO4, 100mM sodium acetate pH4-4.5	Resolution 3.80 Å R-free 0.290
8FOI Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex with GABA and allopregnanolone Deposited 2022-12-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric	Chain A 1–455（455 aa） Chain C 1–455（455 aa）	Not recorded	PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D12 DODECANE × 17 OCT N-OCTANE × 17 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y4B allopregnanolone × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.50 Å
8G4N Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex with GABA, Zolpidem, and endogenous neurosteroids Deposited 2023-02-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric	Chain A 1–455（455 aa） Chain C 1–455（455 aa）	Not recorded	Y4B allopregnanolone × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D12 DODECANE × 17 OCT N-OCTANE × 17 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R5R Zolpidem × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.67 Å
8G4O Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex with didesethylflurazepam and endogenous GABA Deposited 2023-02-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric	Chain A 1–455（455 aa） Chain C 1–455（455 aa）	Not recorded	ABU GAMMA-AMINO-BUTANOIC ACID × 2 YNL (5M)-1-(2-aminoethyl)-7-chloro-5-(2-fluorophenyl)-1,3-dihydro-2H-1,4-benzodiazepin-2-one × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 6 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.06 Å
8G4X Native GABA-A receptor from the mouse brain, meta-alpha1-alpha3-beta2-gamma2 subtype, in complex with GABA and allopregnanolone Deposited 2023-02-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 1–455（455 aa）	Not recorded	PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Y4B allopregnanolone × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.56 Å
8G5F Native GABA-A receptor from the mouse brain, ortho-alpha1-alpha3-beta2-gamma2 subtype, in complex with GABA and allopregnanolone Deposited 2023-02-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric	Chain C 1–455（455 aa）	Not recorded	PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 Y4B allopregnanolone × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.64 Å
8G5G Native GABA-A receptor from the mouse brain, meta-alpha1-alpha3-beta2-gamma2 subtype, in complex with GABA, Zolpidem, and endogenous neurosteroid Deposited 2023-02-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric	Chain A 1–455（455 aa）	Not recorded	PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 Y4B allopregnanolone × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R5R Zolpidem × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.94 Å
8G5H Native GABA-A receptor from the mouse brain, ortho-alpha1-alpha3-beta2-gamma2 subtype, in complex with GABA, Zolpidem, and endogenous neurosteroid Deposited 2023-02-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric	Chain C 1–455（455 aa）	Not recorded	ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R5R Zolpidem × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 Y4B allopregnanolone × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.89 Å

9 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	GBRA1_MOUSE
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 290–328； UniProt 417–455 Author chain B； PDBConstruct 290–328； UniProt 417–455 Author chain C； PDBConstruct 290–328； UniProt 417–455 Author chain D； PDBConstruct 290–328； UniProt 417–455 Author chain E； PDBConstruct 290–328； UniProt 417–455

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

Mus musculus

UniProt Q7NDN8

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Insufficient information Homooligomer Protein × 5 PDB declaration: pentameric（5） Consistent with protein copy count	Chain A； UniProt 44–236 Chain B； UniProt 44–236 Chain C； UniProt 44–236 Chain D； UniProt 44–236 Chain E； UniProt 44–236	Not recorded	ACT ACETATE ION × 8 CL CHLORIDE ION × 5 A8W Pregnenolone sulfate × 5 Y01 CHOLESTEROL HEMISUCCINATE × 5	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 4.5；289 K；12-16% PEG 4000, 100 mM NaCl, 100 mM Li2S04, 100 mM sodium acetate pH 4-5	Resolution 3.10 Å R-free 0.246

Other States of the Same Protein in the Database

Other PDB	Difference from Current Entry 5OSC	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
2XQ3 Pentameric ligand gated ion channel GLIC in complex with Br-lidocaine Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	BR BROMIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG 4000, 50 MM CH3COONA, 200 MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K.	Resolution 3.50 Å R-free 0.257
2XQ4 Pentameric ligand gated ion channel GLIC in complex with tetramethylarsonium (TMAs) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	ARS ARSENIC × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG 4000, 50 MM CH3COONA, 200 MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K.	Resolution 3.60 Å R-free 0.272
2XQ5 Pentameric ligand gated ion channel GLIC in complex with tetraethylarsonium (TEAs) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	ARS ARSENIC × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG 4000, 50 MM CH3COONA, 200 MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K.	Resolution 3.50 Å R-free 0.266
2XQ6 Pentameric ligand gated ion channel GLIC in complex with cesium ion (Cs+) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	CS CESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG4000, 50MM CH3COONA, 225MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP	Resolution 3.70 Å R-free 0.258
2XQ7 Pentameric ligand gated ion channel GLIC in complex with cadmium ion (Cd2+) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	CD CADMIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277.15 K；9% PEG 4000, 50MM CH3COONA, 200MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP.	Resolution 3.40 Å R-free 0.266
2XQ8 Pentameric ligand gated ion channel GLIC in complex with zinc ion (Zn2+) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	ZN ZINC ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG4000, 50MM CH3COONA, 200MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP	Resolution 3.60 Å R-free 0.248
2XQ9 Pentameric ligand gated ion channel GLIC mutant E221A in complex with tetraethylarsonium (TEAs) Deposited 2010-09-01	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	ARS ARSENIC × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；9% PEG 4000, 50MM CH3COONA, 200MM (NH4)2SO4, PH 4.0, VAPOR DIFFUSION, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP.	Resolution 3.20 Å R-free 0.257
2XQA Pentameric ligand gated ion channel GLIC in complex with tetrabutylantimony (TBSb) Deposited 2010-09-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：RESIDUES 43-359 Chain B 43–359（317 aa） Fragment：RESIDUES 43-359 Chain C 43–359（317 aa） Fragment：RESIDUES 43-359 Chain D 43–359（317 aa） Fragment：RESIDUES 43-359 Chain E 43–359（317 aa） Fragment：RESIDUES 43-359	Not recorded	SB ANTIMONY (III) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277.15 K；9% PEG4000, 50MM CH3COONA, 200MM (NH4)2SO4, PH4.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277.15K, VAPOR DIFFUSION, HANGING DROP	Resolution 3.70 Å R-free 0.274
3EAM An open-pore structure of a bacterial pentameric ligand-gated ion channel Deposited 2008-08-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP residues 43-359 Chain B 43–359（317 aa） Fragment：UNP residues 43-359 Chain C 43–359（317 aa） Fragment：UNP residues 43-359 Chain D 43–359（317 aa） Fragment：UNP residues 43-359 Chain E 43–359（317 aa） Fragment：UNP residues 43-359	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；298 K；PEG 4000, NH4SCN, NaAc, pH 4.6, vapor diffusion, hanging drop, temperature 298K	Resolution 2.90 Å R-free 0.231
3EHZ X-ray structure of the pentameric ligand gated ion channel of Gloebacter violaceus (GLIC) in a presumptive open conformation Deposited 2008-09-15	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 50–359（310 aa） Fragment：UNP residues 50-359 Chain B 50–359（310 aa） Fragment：UNP residues 50-359 Chain C 50–359（310 aa） Fragment：UNP residues 50-359 Chain D 50–359（310 aa） Fragment：UNP residues 50-359 Chain E 50–359（310 aa） Fragment：UNP residues 50-359	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277.15 K；9% PEG4000, 50mM CH3COONa, 200mM (NH4)2SO4, pH4.0, vapor diffusion, hanging drop, temperature 277.15K, VAPOR DIFFUSION, HANGING DROP	Resolution 3.10 Å R-free 0.266
3EI0 Structure of the E221A mutant of the Gloebacter violaceus pentameric ligand gated ion channnel (GLIC) Deposited 2008-09-15	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 50–359（310 aa） Fragment：UNP residues 50-359 Chain B 50–359（310 aa） Fragment：UNP residues 50-359 Chain C 50–359（310 aa） Fragment：UNP residues 50-359 Chain D 50–359（310 aa） Fragment：UNP residues 50-359 Chain E 50–359（310 aa） Fragment：UNP residues 50-359	Mutation：E221A Mutation：E221A Mutation：E221A Mutation：E221A Mutation：E221A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；277.15 K；9% PEG4000, 50mM CH3COONa, 500mM (NH4)2SO4, pH4.0, vapor diffusion, hanging drop, temperature 277.15K, VAPOR DIFFUSION, HANGING DROP	Resolution 3.50 Å R-free 0.276
3IGQ Crystal structure of the extracellular domain of a bacterial pentameric ligand-gated ion channel Deposited 2009-07-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain A 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235 Chain B 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235 Chain C 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235	Mutation：F116G, Y119T, P120E, F121S Mutation：F116G, Y119T, P120E, F121S Mutation：F116G, Y119T, P120E, F121S	ACY ACETIC ACID × 6 NA SODIUM ION × 6 HG MERCURY (II) ION × 6 CL CHLORIDE ION × 6	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 2.30 Å R-free 0.255
3IGQ Crystal structure of the extracellular domain of a bacterial pentameric ligand-gated ion channel Deposited 2009-07-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric	Chain D 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235 Chain E 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235 Chain F 44–235（192 aa） Fragment：extracellular N-terminal fragment, UNP residues 44-235	Mutation：F116G, Y119T, P120E, F121S Mutation：F116G, Y119T, P120E, F121S Mutation：F116G, Y119T, P120E, F121S	ACY ACETIC ACID × 6 NA SODIUM ION × 6 HG MERCURY (II) ION × 6 CL CHLORIDE ION × 6	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 2.30 Å R-free 0.255
3LSV Structure of the A237F mutant of the pentameric ligand gated ion channel from Gloeobacter Violaceus Deposited 2010-02-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Mutation：A279F Mutation：A279F Mutation：A279F Mutation：A279F Mutation：A279F	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；PEG 4000 ~25%, NaSCN ~400 mM, NaAc ph 4 100 mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.15 Å R-free 0.224
3P4W Structure of desflurane bound to a pentameric ligand-gated ion channel, GLIC Deposited 2010-10-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：residues in UNP 44-359 Chain B 44–359（316 aa） Fragment：residues in UNP 44-359 Chain C 44–359（316 aa） Fragment：residues in UNP 44-359 Chain D 44–359（316 aa） Fragment：residues in UNP 44-359 Chain E 44–359（316 aa） Fragment：residues in UNP 44-359	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 DSF (2S)-2-(difluoromethoxy)-1,1,1,2-tetrafluoroethane × 5 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.20 Å R-free 0.206
3P50 Structure of propofol bound to a pentameric ligand-gated ion channel, GLIC Deposited 2010-10-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：residues in UNP 44-359 Chain B 44–359（316 aa） Fragment：residues in UNP 44-359 Chain C 44–359（316 aa） Fragment：residues in UNP 44-359 Chain D 44–359（316 aa） Fragment：residues in UNP 44-359 Chain E 44–359（316 aa） Fragment：residues in UNP 44-359	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 5 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.30 Å R-free 0.203
3TLS The GLIC pentameric Ligand-Gated Ion Channel E19'P mutant in a locally-closed conformation (LC2 subtype) Deposited 2011-08-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：E285P Mutation：E285P Mutation：E285P Mutation：E285P Mutation：E285P	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 4；293 K；pH 4, VAPOR DIFFUSION, temperature 293K	Resolution 3.20 Å R-free 0.230
3TLT The GLIC pentameric Ligand-Gated Ion Channel H11'F mutant in a locally-closed conformation (LC1 subtype) Deposited 2011-08-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：H277F Mutation：H277F Mutation：H277F Mutation：H277F Mutation：H277F	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 4；293 K；pH 4, VAPOR DIFFUSION, temperature 293K	Resolution 3.30 Å R-free 0.243
3TLU The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' oxidized mutant in a locally-closed conformation (LC1 subtype) Deposited 2011-08-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C69S/K75C/K290C Mutation：C69S/K75C/K290C Mutation：C69S/K75C/K290C Mutation：C69S/K75C/K290C Mutation：C69S/K75C/K290C	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 4；293 K；pH 4, VAPOR DIFFUSION, temperature 293K	Resolution 2.85 Å R-free 0.223
3TLV The GLIC pentameric Ligand-Gated Ion Channel Loop2-22' oxidized mutant in a locally-closed conformation (LC3 subtype) Deposited 2011-08-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C69S/K75C/L288C Mutation：C69S/K75C/L288C Mutation：C69S/K75C/L288C Mutation：C69S/K75C/L288C Mutation：C69S/K75C/L288C	LMT DODECYL-BETA-D-MALTOSIDE × 1 CL CHLORIDE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 4；293 K；pH 4, VAPOR DIFFUSION, temperature 293K	Resolution 2.90 Å R-free 0.229
3TLW The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' oxidized mutant in a locally-closed conformation (LC2 subtype) Deposited 2011-08-30	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C69S/K75C/N287C Mutation：C69S/K75C/N287C Mutation：C69S/K75C/N287C Mutation：C69S/K75C/N287C Mutation：C69S/K75C/N287C	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 4；293 K；pH 4, VAPOR DIFFUSION, temperature 293K	Resolution 2.60 Å R-free 0.225
3UU3 The GLIC pentameric Ligand-Gated Ion Channel Loop2-20' oxidized mutant in a locally-closed conformation (LC1 subtype) Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C	LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.15 Å R-free 0.272
3UU4 The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant reduced in the crystal in a locally-closed conformation (LC1 subtype) Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C	LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.05 Å R-free 0.241
3UU5 The GLIC pentameric Ligand-Gated Ion Channel Loop2-20' mutant reduced in solution Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C Mutation：C27S,K33C,T244C	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 14 CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.90 Å R-free 0.218
3UU6 The GLIC pentameric Ligand-Gated Ion Channel Loop2-22' mutant reduced in solution Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,L246C Mutation：C27S,K33C,L246C Mutation：C27S,K33C,L246C Mutation：C27S,K33C,L246C Mutation：C27S,K33C,L246C	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.98 Å R-free 0.241
3UU8 The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' mutant reduced in solution Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,K248C Mutation：C27S,K33C,K248C Mutation：C27S,K33C,K248C Mutation：C27S,K33C,K248C Mutation：C27S,K33C,K248C	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.25 Å R-free 0.234
3UUB The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant reduced in solution Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1 CL CHLORIDE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.90 Å R-free 0.221
3UUB The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant reduced in solution Deposited 2011-11-28	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain F 44–359（316 aa） Fragment：UNP residues 44-359 Chain G 44–359（316 aa） Fragment：UNP residues 44-359 Chain H 44–359（316 aa） Fragment：UNP residues 44-359 Chain I 44–359（316 aa） Fragment：UNP residues 44-359 Chain J 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C Mutation：C27S,K33C,N245C	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1 CL CHLORIDE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15% PEG4000, 0.4 M sodium thiocyanate, 0.1 M sodium acetate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.90 Å R-free 0.221
4F8H X-ray Structure of the Anesthetic Ketamine Bound to the GLIC Pentameric Ligand-gated Ion Channel Deposited 2012-05-17	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP residues 43-359 Chain B 43–359（317 aa） Fragment：UNP residues 43-359 Chain C 43–359（317 aa） Fragment：UNP residues 43-359 Chain D 43–359（317 aa） Fragment：UNP residues 43-359 Chain E 43–359（317 aa） Fragment：UNP residues 43-359	Not recorded	RKE (R)-ketamine × 5 LMD tetradecyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；10-12% PEG 4000, 225 mM ammonium sulfate, 50 mM sodium acetate buffer (pH 3.9 - 4.1), VAPOR DIFFUSION, SITTING DROP, temperature 277K	Resolution 2.99 Å R-free 0.219
4HFB The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant (Apo) Deposited 2012-10-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A	ACT ACETATE ION × 10 CL CHLORIDE ION × 5 NA SODIUM ION × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.75 Å R-free 0.222
4HFC The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to 2-bromo-ethanol Deposited 2012-10-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：unp residues 44-359 Chain B 44–359（316 aa） Fragment：unp residues 44-359 Chain C 44–359（316 aa） Fragment：unp residues 44-359 Chain D 44–359（316 aa） Fragment：unp residues 44-359 Chain E 44–359（316 aa） Fragment：unp residues 44-359	Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A	LMT DODECYL-BETA-D-MALTOSIDE × 6 BRJ 2-BROMOETHANOL × 5 CL CHLORIDE ION × 7 NA SODIUM ION × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 ACT ACETATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate pH4, 0.4M NaSCN, 200 mM 2-bromo-ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.05 Å R-free 0.220
4HFD The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to bromoform Deposited 2012-10-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A	LMT DODECYL-BETA-D-MALTOSIDE × 6 MBR TRIBROMOMETHANE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 2% bromoform, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.10 Å R-free 0.208
4HFE The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant complexed to ethanol Deposited 2012-10-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：unp residues 44-359 Chain B 44–359（316 aa） Fragment：unp residues 44-359 Chain C 44–359（316 aa） Fragment：unp residues 44-359 Chain D 44–359（316 aa） Fragment：unp residues 44-359 Chain E 44–359（316 aa） Fragment：unp residues 44-359	Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A Mutation：F280A	ACT ACETATE ION × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 EOH ETHANOL × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 UNL UNKNOWN LIGAND × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 0.2M Ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.80 Å R-free 0.215
4HFH The GLIC pentameric Ligand-Gated Ion Channel (wild-type) complexed to bromoform Deposited 2012-10-05	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 UNL UNKNOWN LIGAND × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 MBR TRIBROMOMETHANE × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 2% bromoform, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.65 Å R-free 0.213
4HFI The GLIC pentameric Ligand-Gated Ion Channel at 2.4 A resolution Deposited 2012-10-05	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12-15% PEG4K, 0.1M Na Acetate pH4, 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 2.40 Å R-free 0.216
4IL4 The pentameric ligand-gated ion channel GLIC in complex with Se-DDM Deposited 2012-12-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Not recorded	ACT ACETATE ION × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LSM dodecyl 4-O-alpha-D-glucopyranosyl-1-seleno-beta-D-glucopyranoside × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；12.5-15% PEG4000, 0.1M NaAcetate pH4, 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.30 Å R-free 0.209
4IL9 The pentameric ligand-gated ion channel GLIC A237F in complex with bromide Deposited 2012-12-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–358（315 aa） Fragment：UNP residues 44-358 Chain B 44–358（315 aa） Fragment：UNP residues 44-358 Chain C 44–358（315 aa） Fragment：UNP residues 44-358 Chain D 44–358（315 aa） Fragment：UNP residues 44-358 Chain E 44–358（315 aa） Fragment：UNP residues 44-358	Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 UNL UNKNOWN LIGAND × 10 BR BROMIDE ION × 12 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；290 K；12-15% PEG 4K, 0.1M Na Acetate pH 4, 0.2M NaSCN, 0.2M NaBr, VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 2.83 Å R-free 0.214
4ILA The pentameric ligand-gated ion channel GLIC A237F in complex with Cesium Deposited 2012-12-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–358（315 aa） Fragment：UNP residues 44-358 Chain B 44–358（315 aa） Fragment：UNP residues 44-358 Chain C 44–358（315 aa） Fragment：UNP residues 44-358 Chain D 44–358（315 aa） Fragment：UNP residues 44-358 Chain E 44–358（315 aa） Fragment：UNP residues 44-358	Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 UNL UNKNOWN LIGAND × 10 CS CESIUM ION × 27 CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；290 K；12-15% PEG 4K, 0.1M Na Acetate pH 4, 0.2M NaSCN, 0.2M CsCl, VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 3.50 Å R-free 0.208
4ILB The pentameric ligand-gated ion channel GLIC A237F in complex with Rubidium Deposited 2012-12-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–358（315 aa） Fragment：UNP residues 44-358 Chain B 44–358（315 aa） Fragment：UNP residues 44-358 Chain C 44–358（315 aa） Fragment：UNP residues 44-358 Chain D 44–358（315 aa） Fragment：UNP residues 44-358 Chain E 44–358（315 aa） Fragment：UNP residues 44-358	Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F Mutation：A237F	LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 UNL UNKNOWN LIGAND × 10 CL CHLORIDE ION × 5 NA SODIUM ION × 6 RB RUBIDIUM ION × 1 ACT ACETATE ION × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；290 K；12-15% PEG 4K, 0.1M Na Acetate pH4, 0.2M NaSCN, 0.2M RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 3.15 Å R-free 0.226
4ILC The GLIC pentameric ligand-gated ion channel in complex with sulfates Deposited 2012-12-29	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–358（315 aa） Fragment：UNP residues 44-358 Chain B 44–358（315 aa） Fragment：UNP residues 44-358 Chain C 44–358（315 aa） Fragment：UNP residues 44-358 Chain D 44–358（315 aa） Fragment：UNP residues 44-358 Chain E 44–358（315 aa） Fragment：UNP residues 44-358	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 UNL UNKNOWN LIGAND × 10 CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 6 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；290 K；12-15% PEG 4K, 0.1M Na Acetate pH4, 0.2M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 290K	Resolution 2.99 Å R-free 0.236
4IRE Crystal structure of GLIC with mutations at the loop C region Deposited 2013-01-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP residues 43-359 Chain B 43–359（317 aa） Fragment：UNP residues 43-359 Chain C 43–359（317 aa） Fragment：UNP residues 43-359 Chain D 43–359（317 aa） Fragment：UNP residues 43-359 Chain E 43–359（317 aa） Fragment：UNP residues 43-359	Mutation：D91N, E177Q, D178N Mutation：D91N, E177Q, D178N Mutation：D91N, E177Q, D178N Mutation：D91N, E177Q, D178N Mutation：D91N, E177Q, D178N	LMD tetradecyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 10 OXL OXALATE ION × 17 ACT ACETATE ION × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 3.9；277 K；10-12% PEG 4000, 225 mM ammonium sulfate, 50 mM sodium acetate buffer (pH 3.9 - 4.1), VAPOR DIFFUSION, SITTING DROP, temperature 277K	Resolution 3.19 Å R-free 0.243
4LMJ GLIC Liganded-closed-channel Conformation, Mutant T25'A Deposited 2013-07-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：T291A Mutation：T291A Mutation：T291A Mutation：T291A Mutation：T291A	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1 NA SODIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.44 Å R-free 0.255
4LMK GLIC Liganded-closed-channel Conformation, Mutant Y27'A Deposited 2013-07-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：T293A Mutation：T293A Mutation：T293A Mutation：T293A Mutation：T293A	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1 NA SODIUM ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.22 Å R-free 0.238
4LML GLIC double mutant I9'A T25'A Deposited 2013-07-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：I275A,T291A Mutation：I275A,T291A Mutation：I275A,T291A Mutation：I275A,T291A Mutation：I275A,T291A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；10-12% PEG4000, 225 mM ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 3.80 Å R-free 0.302
4NPP The GLIC-His10 wild-type structure in equilibrium between the open and locally-closed (LC) forms Deposited 2013-11-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	NI NICKEL (II) ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；15-20% PEG2kmme, 50mM NiCl2, 0.1M Na Acetate pH4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 3.35 Å R-free 0.248
4NPQ The resting-state conformation of the GLIC ligand-gated ion channel Deposited 2013-11-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；13-15% PEG4000, 200mM KSCN, 10mM CaCl2, 3% Trimethylamine-N-oxyde dihydrate, 0.1M Na Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 4.35 Å R-free 0.244
4NPQ The resting-state conformation of the GLIC ligand-gated ion channel Deposited 2013-11-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain F 44–359（316 aa） Chain G 44–359（316 aa） Chain H 44–359（316 aa） Chain I 44–359（316 aa） Chain J 44–359（316 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；13-15% PEG4000, 200mM KSCN, 10mM CaCl2, 3% Trimethylamine-N-oxyde dihydrate, 0.1M Na Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 4.35 Å R-free 0.244
4NPQ The resting-state conformation of the GLIC ligand-gated ion channel Deposited 2013-11-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain K 44–359（316 aa） Chain L 44–359（316 aa） Chain M 44–359（316 aa） Chain N 44–359（316 aa） Chain O 44–359（316 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；13-15% PEG4000, 200mM KSCN, 10mM CaCl2, 3% Trimethylamine-N-oxyde dihydrate, 0.1M Na Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 4.35 Å R-free 0.244
4NPQ The resting-state conformation of the GLIC ligand-gated ion channel Deposited 2013-11-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain P 44–359（316 aa） Chain Q 44–359（316 aa） Chain R 44–359（316 aa） Chain S 44–359（316 aa） Chain T 44–359（316 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；13-15% PEG4000, 200mM KSCN, 10mM CaCl2, 3% Trimethylamine-N-oxyde dihydrate, 0.1M Na Hepes pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 4.35 Å R-free 0.244
4QH1 The GLIC pentameric Ligand-Gated Ion Channel (wild-type) in complex with bromoacetate Deposited 2014-05-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	BR BROMIDE ION × 5 BXA bromoacetic acid × 10 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；291 K；400 mM Sodium Isothiocyanate, 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.40 Å R-free 0.225
4QH4 The GLIC pentameric Ligand-Gated Ion Channel (wild-type) crystallized in acetate buffer at pH3 Deposited 2014-05-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 ACT ACETATE ION × 3 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；291 K；400 mM Sodium Isothiocyanate 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000, 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.20 Å R-free 0.224
4QH5 The GLIC pentameric Ligand-Gated Ion Channel (wild-type) crystallized in phosphate buffer Deposited 2014-05-26	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 CL CHLORIDE ION × 5 NA SODIUM ION × 4 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；291 K；400 mM Sodium Isothiocyanate, 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000, 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K	Resolution 3.00 Å R-free 0.216
4X5T alpha 1 glycine receptor transmembrane structure fused to the extracellular domain of GLIC Deposited 2014-12-05	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–235（192 aa） Chain B 44–235（192 aa） Chain C 44–235（192 aa） Chain D 44–235（192 aa） Chain E 44–235（192 aa）	Not recorded	NI NICKEL (II) ION × 6 CL CHLORIDE ION × 6 ACT ACETATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 3；291 K；16 % PEG 2000MME, 50 mM NiCl2, 4% DMSO, 11% ethylene glycol and 0.1 M NaAcetate pH 3.0	Resolution 3.50 Å R-free 0.270
4YEU ELIC-GLIC chimera in the resting conformation Deposited 2015-02-24	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 236–357（122 aa） Chain B 236–357（122 aa） Chain C 236–357（122 aa） Chain D 236–357（122 aa） Chain E 236–357（122 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；277 K；PEG 4000, Ammonium Sulfate, Sodium ADA	Resolution 4.60 Å R-free 0.248
4ZZB The GLIC pentameric Ligand-Gated Ion Channel Locally-closed form complexed to xenon Deposited 2015-05-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S K33C L246C Mutation：C27S K33C L246C Mutation：C27S K33C L246C Mutation：C27S K33C L246C Mutation：C27S K33C L246C	LMT DODECYL-BETA-D-MALTOSIDE × 1 CL CHLORIDE ION × 5 NA SODIUM ION × 5 ACT ACETATE ION × 6 XE XENON × 21	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；290 K；12-15 % PEG4000 0.1M Na Acetate pH4 0.4M NaSCN 15% glycerol 5% DMSO	Resolution 3.40 Å R-free 0.223
4ZZC The GLIC pentameric Ligand-Gated Ion Channel open form complexed to xenon Deposited 2015-05-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: Pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 5 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 XE XENON × 30	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；290 K；12-15% PEG4000 0.1M Na Acetate pH4 0.4M NaSCN 15% glycerol 5% DMSO	Resolution 3.10 Å R-free 0.228
5HCJ Cationic Ligand-Gated Ion Channel Deposited 2016-01-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S, K33C, L246C Mutation：C27S, K33C, L246C Mutation：C27S, K33C, L246C Mutation：C27S, K33C, L246C Mutation：C27S, K33C, L246C	LMT DODECYL-BETA-D-MALTOSIDE × 1 CL CHLORIDE ION × 5 NA SODIUM ION × 1 MBR TRIBROMOMETHANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；PEG4000 12-15%, NaSCN 0.4M, 15% glycerol, NaAcetate pH 4 0.1M	Resolution 2.95 Å R-free 0.218
5HCM The GLIC pentameric Ligand-Gated Ion Channel 2-21' cross-linked mutant complexed to bromoform Deposited 2016-01-04	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP residues 44-359 Chain B 44–359（316 aa） Fragment：UNP residues 44-359 Chain C 44–359（316 aa） Fragment：UNP residues 44-359 Chain D 44–359（316 aa） Fragment：UNP residues 44-359 Chain E 44–359（316 aa） Fragment：UNP residues 44-359	Mutation：C27S, K33C, N245C Mutation：C27S, K33C, N245C Mutation：C27S, K33C, N245C Mutation：C27S, K33C, N245C Mutation：C27S, K33C, N245C	LMT DODECYL-BETA-D-MALTOSIDE × 1 MBR TRIBROMOMETHANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；PEG 4000 12-15%, 0.1 M Na Acetate pH4, 0.4M NaSCN, 15% glycerol	Resolution 3.15 Å R-free 0.240
5HEG Pentameric ligand-gated ion channel GLIC mutant P246G Deposited 2016-01-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Mutation：P246G Mutation：P246G Mutation：P246G Mutation：P246G Mutation：P246G	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；277 K；Ammonium sulfate, PEG 4000	Resolution 3.21 Å R-free 0.258
5HEH Pentameric ligand-gated ion channel GLIC mutant P246A Deposited 2016-01-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Mutation：P246A Mutation：P246A Mutation：P246A Mutation：P246A Mutation：P246A	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；277 K；PEG 4000, Ammonium Sulfate	Resolution 3.30 Å R-free 0.280
5IUX GLIC-V135C bimane labelled X-ray structure Deposited 2016-03-18	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 15 ACT ACETATE ION × 10 6E3 2,3,5,6-tetramethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione × 2 BR BROMIDE ION × 5 NA SODIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；12% PEG4K, 15% DMSO, 100 mM Acetate de Sodium pH4, 200 mM Sodium isothiocyanate, 2% DMSO	Resolution 2.60 Å R-free 0.237
5J0Z Crystal structure of GLIC in complex with DHA Deposited 2016-03-28	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–357（311 aa） Chain B 47–357（311 aa） Chain C 47–357（311 aa） Chain D 47–357（311 aa） Chain E 47–357（311 aa）	Not recorded	CL CHLORIDE ION × 3 LMT DODECYL-BETA-D-MALTOSIDE × 6 SO4 SULFATE ION × 6 ACT ACETATE ION × 2 HXA DOCOSA-4,7,10,13,16,19-HEXAENOIC ACID × 5 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；277 K；225 mM ammonium sulfate, 50 mM sodium acetate pH 4.0, 7.5-10% PEG 4000	Resolution 3.25 Å R-free 0.261
5L47 X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with cyanoselenobarbital (seleniated barbiturate) Deposited 2016-05-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 5 ACT ACETATE ION × 5 NA SODIUM ION × 4 6JA 2-[5-ethyl-2,4,6-tris(oxidanylidene)-1,3-diazinan-5-yl]ethyl selenocyanate × 1 D12 DODECANE × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；100 mM Na Acetate pH4, 200 mM Na SCN, 12-15% PEG4000, 3% DMSO, 16% glycerol	Resolution 3.30 Å R-free 0.230
5L4E X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with thiopental Deposited 2016-05-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	D12 DODECANE × 1 CL CHLORIDE ION × 5 EDP 5-ethyl-5-[(2R)-pentan-2-yl]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；291 K；100 mM NaAcetate pH4 200 NaSCN 12-15% PEG4000 16% glycerol 3%DMSO	Resolution 3.50 Å R-free 0.249
5L4H X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with 5-(2-BROMO-ETHYL)-5-ETHYL-PYRIMIDINE-2,4,6-TRIONE (brominated barbiturate) Deposited 2016-05-25	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1 6JW 5-(2-bromoethyl)-5-ethyl-1,3-diazinane-2,4,6-trione × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；100 mM NaAcetate pH4, 200 mM NaSCN, 12-15% PEG4000, 16% glycerol, 3% DMSO	Resolution 3.30 Å R-free 0.235
5MUO X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with propofol Deposited 2017-01-13	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain B 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain C 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain D 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain E 44–359（316 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	LMT DODECYL-BETA-D-MALTOSIDE × 1 CL CHLORIDE ION × 5 ACT ACETATE ION × 4 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K	Resolution 3.19 Å R-free 0.204
5MUR X-ray structure of the F14'A mutant of GLIC in complex with propofol Deposited 2017-01-13	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP residues 43-359 Chain B 43–359（317 aa） Fragment：UNP residues 43-359 Chain C 43–359（317 aa） Fragment：UNP residues 43-359 Chain D 43–359（317 aa） Fragment：UNP residues 43-359 Chain E 43–359（317 aa） Fragment：UNP residues 43-359	Not recorded	ACT ACETATE ION × 10 CL CHLORIDE ION × 5 NA SODIUM ION × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；100 mM Na acetate pH4 12-15% PEG4K 200 mM NaSCN 16% glycerol 2% DMSO	Resolution 3.10 Å R-free 0.204
5MVM X-ray structure of the F14'A -N15'A double mutant of GLIC in complex with propofol Deposited 2017-01-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 6 ACT ACETATE ION × 10 CL CHLORIDE ION × 5 NA SODIUM ION × 7 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；400mM NaSCN 100mM NaAcetate pH4 12-15% PEG4K 16% glycerol 2% DMSO	Resolution 3.10 Å R-free 0.208
5MVN X-ray structure of the M205W mutant of GLIC in complex with propofol Deposited 2017-01-16	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；400 mM NaSCN 100 mM NaAcetate pH4 12-15 % PEG4K 16 % glycerol 2 % DMSO	Resolution 3.49 Å R-free 0.232
5MZQ X-ray structure of the M205W mutant of GLIC in complex with bromoform Deposited 2017-02-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PC8 1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 5 2IL (3R)-3-(dodecanoyloxy)tetradecanoic acid × 5 D12 DODECANE × 11 CL CHLORIDE ION × 7 NA SODIUM ION × 6 MBR TRIBROMOMETHANE × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；100 mM NaAct pH4 400 mM NaSCN 16% glycerol 2% DMSO 12-15 % PEG4K	Resolution 2.80 Å R-free 0.219
5MZR X-ray structure of the H235Q mutant of GLIC in complex with propofol Deposited 2017-02-01	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Mutation：H235Q Mutation：H235Q Mutation：H235Q Mutation：H235Q Mutation：H235Q	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；100 mM NaAct pH4 400 mM NaSCN 16% glycerol 2% DMSO 12-15% PEG4K	Resolution 2.65 Å R-free 0.210
5MZT X-ray structure of the H235Q mutant of GLIC in complex with bromoform Deposited 2017-02-01	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：H235Q Chain B 43–359（317 aa） Fragment：H235Q Chain C 43–359（317 aa） Fragment：H235Q Chain D 43–359（317 aa） Fragment：H235Q Chain E 43–359（317 aa） Fragment：H235Q	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MBR TRIBROMOMETHANE × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；294 K；100 mM NaAct pH 4 400 mM NaSCN 16% glycerol 2% DMSO	Resolution 2.65 Å R-free 0.211
5NJY X-ray structure of the H235Q mutant of GLIC Deposited 2017-03-30	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；400 mM NaSCN 100 mM Na Acetate pH 4 12-15% PEG4000 2% DMSO 16% Glycerol	Resolution 2.95 Å R-free 0.243
5NKJ X-ray structure of the N239C mutant of GLIC Deposited 2017-03-31	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain B 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain C 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain D 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain E 43–359（317 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K	Resolution 3.74 Å R-free 0.256
5OSA GLIC-GABAAR alpha1 chimera crystallized at pH4.6 Deposited 2017-08-17	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–236（193 aa） Chain B 44–236（193 aa） Chain C 44–236（193 aa） Chain D 44–236（193 aa） Chain E 44–236（193 aa）	Not recorded	ACT ACETATE ION × 8 CL CHLORIDE ION × 7 D12 DODECANE × 8 Y01 CHOLESTEROL HEMISUCCINATE × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；289.15 K；100 mM NaCl, 100 mM sodium acetate pH 4.6, 12 % PEG 6000	Resolution 2.75 Å R-free 0.229
5OSB GLIC-GABAAR alpha1 chimera crystallized in complex with THDOC at pH4.5 Deposited 2017-08-17	Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Insufficient information Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–236（193 aa） Chain B 44–236（193 aa） Chain C 44–236（193 aa） Chain D 44–236（193 aa） Chain E 44–236（193 aa）	Not recorded	A8Z Tetrahydrodeoxycorticosterone × 5 ACT ACETATE ION × 5 CL CHLORIDE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.5；289 K；12-16% PEG4000, 100mM NaCl, 100 mM Li2SO4, 100mM sodium acetate pH4-4.5	Resolution 3.80 Å R-free 0.290
5V6N Crystal Structure of the highly open channel-stabilized mutant C27S + K33C + I9'A + N21'C of GLIC under reducing conditions. Deposited 2017-03-17	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 50–359（310 aa） Fragment：unp residues 50-359 Chain B 50–359（310 aa） Fragment：unp residues 50-359 Chain C 50–359（310 aa） Fragment：unp residues 50-359 Chain D 50–359（310 aa） Fragment：unp residues 50-359 Chain E 50–359（310 aa） Fragment：unp residues 50-359	Mutation：C69S, K75C, I275A, N287C Mutation：C69S, K75C, I275A, N287C Mutation：C69S, K75C, I275A, N287C Mutation：C69S, K75C, I275A, N287C Mutation：C69S, K75C, I275A, N287C	LMT DODECYL-BETA-D-MALTOSIDE × 5 NA SODIUM ION × 1 SO4 SULFATE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；200-250 mM (NH4)2SO4, 10-12% (wt/vol) PEG-4000 50 mM sodium acetate, pH 3.9-4.2	Resolution 3.35 Å R-free 0.284
5V6O Crystal Structure of the highly open channel-stabilized mutant G-2'I + I9'A of GLIC Deposited 2017-03-17	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 50–359（310 aa） Fragment：unp residues 50-359 Chain B 50–359（310 aa） Fragment：unp residues 50-359 Chain C 50–359（310 aa） Fragment：unp residues 50-359 Chain D 50–359（310 aa） Fragment：unp residues 50-359 Chain E 50–359（310 aa） Fragment：unp residues 50-359	Mutation：C27S, K33C Mutation：C27S, K33C Mutation：C27S, K33C Mutation：C27S, K33C Mutation：C27S, K33C	LMT DODECYL-BETA-D-MALTOSIDE × 6 CL CHLORIDE ION × 5 NA SODIUM ION × 1 SO4 SULFATE ION × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；Sodium Acetate 50 mM pH 3.9-4.2 Ammonium Sulfate 200-250 mM PEG 4000 10-12%	Resolution 3.12 Å R-free 0.280
6EMX X-ray structure of the N15'C mutant of GLIC in complex with bromoform Deposited 2017-10-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 4 NA SODIUM ION × 6 MBR TRIBROMOMETHANE × 6	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；291 K；12-14% PEGK 400mM NaSCN 100 mM NaAcetate pH 4 15% glycerol 3% DMSO	Resolution 3.20 Å R-free 0.268
6F0I GLIC mutant E26A Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；291.15 K；PEG 4000, 15% Glycerol, 0.1M Sodium acetate, 0.4M Thiocyanate, 3% DMSO	Resolution 3.00 Å R-free 0.223
6F0J GLIC mutant E26A Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；292 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 3.15 Å R-free 0.222
6F0M GLIC mutant E35Q Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 2.65 Å R-free 0.222
6F0N GLIC mutant E82A Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 3.20 Å R-free 0.233
6F0R GLIC mutant E82Q Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 CL CHLORIDE ION × 4 LMT DODECYL-BETA-D-MALTOSIDE × 6 NA SODIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 2.50 Å R-free 0.254
6F0U GLIC mutant E35A Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 2.35 Å R-free 0.233
6F0V GLIC mutant E82Q Deposited 2017-11-20	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 200 mM Sodium isothiocyanate, 3% DMSO	Resolution 2.85 Å R-free 0.221
6F0Z GLIC mutant D88N Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；12% PEG4K, 15% glycerol, 100 mM Acetate de Sodium pH4, 400 mM Sodium isothiocyanate, 3% DMSO	Resolution 2.50 Å R-free 0.226
6F10 GLIC mutant D88N Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH4 400 mM NaSCN 12-15% PEG4000 15% glycerol 3%DMSO	Resolution 2.85 Å R-free 0.223
6F11 GLIC mutant D86A Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH4 400 mM NaSCN 12-15% PEG 4000 15% glycerol 3%DMSO	Resolution 2.95 Å R-free 0.215
6F12 GLIC mutant E181A Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 ACT ACETATE ION × 10 LMT DODECYL-BETA-D-MALTOSIDE × 6 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH4 400 NaSCN 12-15% PEG4000 15% glycerol 3%DMSO	Resolution 3.20 Å R-free 0.213
6F13 GLIC mutant E75A Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 14 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH 4 200mM NaSCN 12-15% PEG4000 15% glycerol 3%DMSO	Resolution 2.70 Å R-free 0.223
6F15 GLIC mutant H127Q Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH 4 400mM NaSCN 12-15% PEG4000 165% glycerol 3%DMSO	Resolution 2.85 Å R-free 0.229
6F16 GLIC mutant H277Q Deposited 2017-11-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；293 K；100 mM NaAcetate pH 4 400 NaSCN 12-15% PEG4000 15% glycerol 3%DMSO	Resolution 2.60 Å R-free 0.219
6F7A Gloeobacter Ligand-gated Ion Channel (GLIC) closed state crystallized in an ultra-swollen lipidic mesophase Deposited 2017-12-07	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–357（311 aa） Chain B 47–357（311 aa） Chain C 47–357（311 aa） Chain D 47–357（311 aa） Chain E 47–357（311 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions LIPIDIC CUBIC PHASE；298 K；0.2 M (NH4)SO4, 0.02M NaCl, 0.02M Na Act 4 pH, 33%v/v PEG200	Resolution 6.00 Å R-free 0.318
6HJ3 Xray structure of GLIC in complex with fumarate Deposited 2018-08-31	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 12 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 FUM FUMARIC ACID × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；30 mM Na fumarate pH 4 12-15% PEG4000 400 mM Na Isothiocyanate 15% glycerol 2% DMSO	Resolution 2.70 Å R-free 0.216
6HJA Xray structure of GLIC in complex with glutarate Deposited 2018-09-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–359（359 aa） Chain B 1–359（359 aa） Chain C 1–359（359 aa） Chain D 1–359（359 aa） Chain E 1–359（359 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 14 CL CHLORIDE ION × 11 D12 DODECANE × 6 GUA GLUTARIC ACID × 5 NA SODIUM ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；100 mM Na-Acetate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 16% Glycerol	Resolution 2.70 Å R-free 0.219
6HJB Xray structure of GLIC in complex with malonate Deposited 2018-09-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 1–359（359 aa） Chain B 1–359（359 aa） Chain C 1–359（359 aa） Chain D 1–359（359 aa） Chain E 1–359（359 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 11 CL CHLORIDE ION × 11 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 MLA MALONIC ACID × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；291 K；100 mM Na-malonate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 16% Glycerol	Resolution 3.00 Å R-free 0.199
6HJI Xray structure of GLIC in complex with crotonate Deposited 2018-09-03	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 12 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 BEO BUTENOIC ACID × 5	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；100 mM Na-crotonate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 15% glycerol	Resolution 2.80 Å R-free 0.204
6HJZ Xray structure of GLIC in complex with succinate Deposited 2018-09-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 SIN SUCCINIC ACID × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；100 mM NaSuccinate pH4 400 mM NaIsothiocynate 12-15% PEG4000 2% DMSO 15% glycerol	Resolution 2.50 Å R-free 0.227
6HJZ Xray structure of GLIC in complex with succinate Deposited 2018-09-04	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain F 43–359（317 aa） Chain G 43–359（317 aa） Chain H 43–359（317 aa） Chain I 43–359（317 aa） Chain J 43–359（317 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 SIN SUCCINIC ACID × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；293 K；100 mM NaSuccinate pH4 400 mM NaIsothiocynate 12-15% PEG4000 2% DMSO 15% glycerol	Resolution 2.50 Å R-free 0.227
6HPP X-ray structure of GLIC in complex with propionate Deposited 2018-09-21	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6 PPI PROPANOIC ACID × 10	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；100 mM Na-propionate, pH 4 400 mM NaIsothiocyanate 12-15% PEG4000 2% DMSO 15% glycreol	Resolution 3.20 Å R-free 0.201
6HY5 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C Deposited 2018-10-19	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–357（311 aa） Chain B 47–357（311 aa） Chain C 47–357（311 aa） Chain D 47–357（311 aa） Chain E 47–357（311 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；291 K；12-15% PEG4K, 0.1M NA ACETATE PH4, 0.4M NASCN, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K	Resolution 2.58 Å R-free 0.205
6HY9 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193M Deposited 2018-10-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain B 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain C 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain D 44–359（316 aa） Fragment：UNP RESIDUES 44-359 Chain E 44–359（316 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；291 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 291K. 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 2.95 Å R-free 0.227
6HYA THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193L Deposited 2018-10-19	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain B 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain C 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain D 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain E 43–359（317 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	CL CHLORIDE ION × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K;12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 3.39 Å R-free 0.212
6HYR THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C+MMTS Deposited 2018-10-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–357（311 aa） Chain B 47–357（311 aa） Chain C 47–357（311 aa） Chain D 47–357（311 aa） Chain E 47–357（311 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF)	LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 3.50 Å R-free 0.241
6HYV THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y119A Deposited 2018-10-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	LMT DODECYL-BETA-D-MALTOSIDE × 11 ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4	Resolution 2.80 Å R-free 0.221
6HYW THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y119F Deposited 2018-10-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 10 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 2.80 Å R-free 0.212
6HYX THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Y197F-P250C Deposited 2018-10-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain B 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain C 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain D 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain E 43–359（317 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	CL CHLORIDE ION × 5 NA SODIUM ION × 7 LMT DODECYL-BETA-D-MALTOSIDE × 1	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K.12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4	Resolution 3.00 Å R-free 0.224
6HYZ THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT K248C Deposited 2018-10-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4	Resolution 3.05 Å R-free 0.234
6HZ0 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT K248A Deposited 2018-10-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 2.75 Å R-free 0.238
6HZ1 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243C Deposited 2018-10-22	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 6	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；291 K；12-15% PEG4K, 0.1M NA ACETATE PH4, 0.4M NASCN, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K	Resolution 2.50 Å R-free 0.216
6HZ3 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243G Deposited 2018-10-22	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain B 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain C 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain D 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain E 43–359（317 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	CL CHLORIDE ION × 5	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K,12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4	Resolution 3.15 Å R-free 0.249
6HZW THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL 2.22 resolution Deposited 2018-10-24	Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Chain B 43–359（317 aa） Chain C 43–359（317 aa） Chain D 43–359（317 aa） Chain E 43–359（317 aa）	Not recorded	ACT ACETATE ION × 10 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 15 CL CHLORIDE ION × 7 NA SODIUM ION × 6 LMT DODECYL-BETA-D-MALTOSIDE × 11	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；291 K；12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 2.22 Å R-free 0.220
6I08 THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243C-I201W Deposited 2018-10-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain B 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain C 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain D 43–359（317 aa） Fragment：UNP RESIDUES 44-359 Chain E 43–359（317 aa） Fragment：UNP RESIDUES 44-359	Mutation：YES Mutation：YES Mutation：YES Mutation：YES Mutation：YES	CL CHLORIDE ION × 5 PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 5 LMT DODECYL-BETA-D-MALTOSIDE × 1 NA SODIUM ION × 7	X-RAY DIFFRACTION X-ray crystallization conditions EVAPORATION；pH 4；293 K；PH 4, VAPOR DIFFUSION, TEMPERATURE 293K 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.	Resolution 3.00 Å R-free 0.228
6ZGD GLIC pentameric ligand-gated ion channel, pH 7 Deposited 2020-06-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；blot for 1.5 s force -1	Resolution 4.10 Å
6ZGJ GLIC pentameric ligand-gated ion channel, pH 5 Deposited 2020-06-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE；blot for 1.5 s force -1	Resolution 3.40 Å
6ZGK GLIC pentameric ligand-gated ion channel, pH 3 Deposited 2020-06-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 3 cryo-EM vitrification conditions Cryogen ETHANE；blot for 1.5 s force -1	Resolution 3.60 Å
8ATG Pentameric ligand-gated ion channel GLIC with bound lipids Deposited 2022-08-23	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 44–359（316 aa） Chain B 44–359（316 aa） Chain C 44–359（316 aa） Chain D 44–359（316 aa） Chain E 44–359（316 aa）	Not recorded	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 25	ELECTRON MICROSCOPY cryo-EM buffer pH 7 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
8I41 Cryo-EM structure of nanodisc (asolectin) reconstituted GLIC at pH 7.5 Deposited 2023-01-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–359（313 aa） Chain B 47–359（313 aa） Chain C 47–359（313 aa） Chain D 47–359（313 aa） Chain E 47–359（313 aa）	Not recorded	PLC DIUNDECYL PHOSPHATIDYL CHOLINE × 25	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.42 Å
8I42 Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 7.5 Deposited 2023-01-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 7.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.92 Å
8I47 Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 5.5 Deposited 2023-01-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 30	ELECTRON MICROSCOPY cryo-EM buffer pH 5.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
8I48 Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in closed state Deposited 2023-01-18	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.74 Å
8JJ3 Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 2.5 Deposited 2023-05-29	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 45	ELECTRON MICROSCOPY cryo-EM buffer pH 2.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.65 Å
8WCQ Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in intermediate state Deposited 2023-09-13	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 40	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.35 Å
8WCR Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in open state Deposited 2023-09-13	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 45	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.74 Å
9LAG Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in iiiii conformation Deposited 2025-01-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 30	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.96 Å
9LAI Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in iiiio conformation Deposited 2025-01-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 24	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.08 Å
9LAJ Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in iiioo conformation Deposited 2025-01-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 23	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9LAK Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in iiooo conformation Deposited 2025-01-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 3 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 19	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.21 Å
9LB9 Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in ioooo conformation Deposited 2025-01-03	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 22	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.16 Å
9LBA Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in ooooo conformation Deposited 2025-01-03	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 35	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.85 Å
9LBB Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in iioio conformation Deposited 2025-01-03	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 10	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.45 Å
9LBC Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC at pH 4 in ioioo conformation Deposited 2025-01-03	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Not recorded	PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 5	ELECTRON MICROSCOPY cryo-EM buffer pH 4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.40 Å
9LBD Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC F116A mutant at pH 2.5 Deposited 2025-01-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Mutation：F116A Mutation：F116A Mutation：F116A Mutation：F116A Mutation：F116A	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 25	ELECTRON MICROSCOPY cryo-EM buffer pH 2.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.14 Å
9LBE Cryo-EM structure of nanodisc (PE:PS:PC) reconstituted GLIC Y251A mutant at pH 2.5 in ioooo conformation Deposited 2025-01-03	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 5 PDB declaration: pentameric	Chain A 47–358（312 aa） Chain B 47–358（312 aa） Chain C 47–358（312 aa） Chain D 47–358（312 aa） Chain E 47–358（312 aa）	Mutation：Y251 Mutation：Y251 Mutation：Y251 Mutation：Y251 Mutation：Y251	CL CHLORIDE ION × 5 PEE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine × 21	ELECTRON MICROSCOPY cryo-EM buffer pH 2.5 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.87 Å

129 other PDB entries and 135 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	GLIC_GLOVI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–193； UniProt 44–236 Author chain B； PDBConstruct 1–193； UniProt 44–236 Author chain C； PDBConstruct 1–193； UniProt 44–236 Author chain D； PDBConstruct 1–193； UniProt 44–236 Author chain E； PDBConstruct 1–193； UniProt 44–236

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	5osc
Deposition date deposition_date	2017-08-17
Structure title title	GLIC-GABAAR alpha1 chimera crystallized in complex with pregnenolone sulfate at pH 4.5
Keywords keywords	GABAA-receptor ion channel Ion transport Extracellular ligand gated ion channel, TRANSPORT PROTEIN; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

▼

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	37.97 Å
Radius of gyration Rg (electron density) rg_electron	37.44 Å
Forward intensity I(0) i0	416033000.00
Molecular weight molecular_weight	180580.0 kDa
Excluded volume excluded_volume	231560 Å³
Envelope volume envelope_volume	293070 Å³
Hydration-shell volume shell_volume	64046 Å³
Envelope diameter envelope_diameter	125.4 Å
Shell Rg shell_rg	44.79 Å
Envelope Rg envelope_rg	37.03 Å
Shape Rg shape_rg	37.43 Å
Total Rg total_rg	37.91 Å
Total atoms total_atoms	13188
Residues n_residues	1560
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

▼

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	123.5 Å
Rg (real space) rg_real	37.87 Å
Rg uncertainty (real space) rg_real_error	1.16 Å
I(0) (real space) i0_real	4.1600e+08
I(0) uncertainty (real space) i0_real_error	7.0210e+06
Rg (reciprocal space) rg_reciprocal	37.94 Å
I(0) (reciprocal space) i0_reciprocal	416100000.0000
Solution quality estimate total_estimate	0.8793
Solution quality rating solution_quality	GOOD a GOOD solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	47.0 Å
Skewness Skewness skewness	0.312
Kurtosis Kurtosis kurtosis	-0.307
Angular range angular_range	— – 0.2100 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	57020000.0000
Real-space data points n_real_points	43
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.848; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.990; Smooth: 0.892

▶

5. Crystallography and Experiment 5. Crystallography & Experiment

▶

6. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1
Molecular weight formula_weight	38644.2 kDa
Source method src_method	man
Copy count entity_count	5

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D,E
Atom count atom_count	13188

QDMVSPPPPIADEPLTVNTGIYLIECYSLDDKAETFKVNAFLSLSWKDRRLAFDPVRSGVRVKTYEPEAIWIPEIRFVNVENARDADVVDISVSPDGTVQYLERFSARVLSPLDFRRYPFDSQTLHIYLIVRSVDTRNIVLAVDLEKVGKNDDVFLTGWDIESFTAVVKPANFALEDRLESKLDYQLRISRQYGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFVVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKSQPARAAKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQHHHHHHHH

来源 Source

来源生物 organism	Mus musculus
通用名 common_name	Mouse
NCBI Class编号 ncbi_taxonomy_id	10090
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 Entity 2 non-polymer

Description description	ACETATE ION
Molecular weight formula_weight	59.0 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 3 Entity 3 non-polymer

Description description	CHLORIDE ION
Molecular weight formula_weight	35.5 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 4 Entity 4 non-polymer

Description description	Pregnenolone sulfate
Molecular weight formula_weight	396.5 kDa
Source method src_method	syn
Copy count entity_count	5

Entity 5 Entity 5 non-polymer

Description description	CHOLESTEROL HEMISUCCINATE
Molecular weight formula_weight	486.7 kDa
Source method src_method	syn
Copy count entity_count	5

▼

7. Fold Classification (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

Domain ID domain_id	5oscA01
Class class	2 — Mainly Beta
Architecture architecture	70 — Distorted Sandwich
Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
Homologous superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

Domain ID domain_id	5oscA02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

Domain ID domain_id	5oscB01
Class class	2 — Mainly Beta
Architecture architecture	70 — Distorted Sandwich
Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
Homologous superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

Domain ID domain_id	5oscB02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

Domain ID domain_id	5oscC01
Class class	2 — Mainly Beta
Architecture architecture	70 — Distorted Sandwich
Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
Homologous superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

Domain ID domain_id	5oscC02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

Domain ID domain_id	5oscD01
Class class	2 — Mainly Beta
Architecture architecture	70 — Distorted Sandwich
Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
Homologous superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

Domain ID domain_id	5oscD02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

Domain ID domain_id	5oscE01
Class class	2 — Mainly Beta
Architecture architecture	70 — Distorted Sandwich
Topology topology	170 — Acetylcholine Binding Protein; Chain: A,
Homologous superfamily homologous superfamily	10 — Neurotransmitter-gated ion-channel ligand-binding domain

Domain ID domain_id	5oscE02
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
Homologous superfamily homologous superfamily	390 — Neurotransmitter-gated ion-channel transmembrane domain

▶

8. Citations (1)

▶

Unit-cell length a length_a	183.310 Å
Unit-cell length b length_b	133.180 Å
Unit-cell length c length_c	161.860 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	102.71 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	20

Space group space_group	C 1 2 1
Space-group number int_tables_number	5
Crystal system crystal_system	monoclinic

Resolution resolution	3.100 Å
Low-resolution limit resolution_low	29.920 Å
R factor r_factor	0.2130
R_work r_work	0.2110
R_free r_free	0.2460

fit	/app/data/saxs_computation/os/5osc/5osc.fit
pddf	/app/data/pr_computation/os/5osc/5osc.pr.out
profile	/app/data/saxs_computation/os/5osc/5osc.dat

GLIC-GABAAR alpha1 chimera crystallized in complex with pregnenolone sulfate at pH 4.5

1. Protein Identity and Related Structures Protein Identity & Related Structures

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

State in the Current Structure

Other States of the Same Protein in the Database

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

State in the Current Structure

Other States of the Same Protein in the Database

Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-2,Gamma-aminobutyric acid receptor subunit alpha-1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 non-polymer

Entity 3 Entity 3 non-polymer

Entity 4 Entity 4 non-polymer

Entity 5 Entity 5 non-polymer

7. Fold Classification (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)