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5uio

structure of DHFR with bound DAP, p-ABG and NADP

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Dihydrofolate reductase

Escherichia coli

UniProt P0ABQ4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein monomer Monomer Protein 1 PYRIMIDINE-2,4-DIAMINE × 1 N-(4-aminobenzene-1-carbonyl)-L-glutamic acid × 1 NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 water × 1 Consistent with protein count
2 Protein monomer Monomer Protein 1 PYRIMIDINE-2,4-DIAMINE × 1 BETA-MERCAPTOETHANOL × 1 FORMIC ACID × 1 water × 1 Consistent with protein count
3 Protein monomer Monomer Protein 1 PYRIMIDINE-2,4-DIAMINE × 1 N-(4-aminobenzene-1-carbonyl)-L-glutamic acid × 1 NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 BETA-MERCAPTOETHANOL × 1 FORMIC ACID × 1 water × 1 Consistent with protein count
4 Protein monomer Monomer Protein 1 PYRIMIDINE-2,4-DIAMINE × 1 N-(4-aminobenzene-1-carbonyl)-L-glutamic acid × 1 NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 BETA-MERCAPTOETHANOL × 1 water × 1 Consistent with protein count
5 Protein monomer Monomer Protein 1 PYRIMIDINE-2,4-DIAMINE × 1 N-(4-aminobenzene-1-carbonyl)-L-glutamic acid × 1 NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 1 BETA-MERCAPTOETHANOL × 1 water × 1 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1dre 1drh 1jom 1ra1 1ra2 1ra3 1ra8 1ra9 1rc4 1rf7 1rg7 1rh3

128 assemblies; showing the first 12

Homodimer
1ddr 1dds 1dhi 1dhj 1dra 1drb 1dyh 1dyi 1dyj 1jol 1rb2 1rb3

20 assemblies; showing the first 12

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
3k74 4eig 4eiz 4eiz 4ej1 4ej1 4fhb 4i13 4i1n

9 assemblies

View Construct and Data Evidence
UniProt name DYR_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–160； UniProt 1–159 Author chain B； PDBConstruct 2–160； UniProt 1–159 Author chain C； PDBConstruct 2–160； UniProt 1–159 Author chain D； PDBConstruct 2–160； UniProt 1–159 Author chain E； PDBConstruct 2–160； UniProt 1–159

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id5uio
Deposition date deposition_date2017-01-14
Structure title titlestructure of DHFR with bound DAP, p-ABG and NADP
Keywords keywordsDHFR, NADP, 2, 4 diaminopyridine, N-(4aminobenzoyl)-L-glutamate, OXIDOREDUCTASE; OXIDOREDUCTASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5uio__assembly_3__model_1

Assembly 3 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5uio__assembly_3__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5uio__assembly_3__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）16.67 Å
Rg (electron density)15.25 Å
Total Rg16.29 Å
Atom count1350
Residues160
Excluded volume23669 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5uio__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 5uio__assembly_2__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 5uio__assembly_3__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 5uio__assembly_4__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 5uio__assembly_5__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (7)

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6. Fold Classification (SCOP + CATH) 10 domains

SCOP 2.08 (5 domains)

Domain ID domain_idd5uioa_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.71 — Dihydrofolate reductase-like
Superfamily Superfamily superfamilyc.71.1 — Dihydrofolate reductase-like
Family Family familyc.71.1.0 — automated matches
Domain ID domain_idd5uiob_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.71 — Dihydrofolate reductase-like
Superfamily Superfamily superfamilyc.71.1 — Dihydrofolate reductase-like
Family Family familyc.71.1.0 — automated matches
Domain ID domain_idd5uioc_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.71 — Dihydrofolate reductase-like
Superfamily Superfamily superfamilyc.71.1 — Dihydrofolate reductase-like
Family Family familyc.71.1.0 — automated matches
Domain ID domain_idd5uiod_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.71 — Dihydrofolate reductase-like
Superfamily Superfamily superfamilyc.71.1 — Dihydrofolate reductase-like
Family Family familyc.71.1.0 — automated matches
Domain ID domain_idd5uioe_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.71 — Dihydrofolate reductase-like
Superfamily Superfamily superfamilyc.71.1 — Dihydrofolate reductase-like
Family Family familyc.71.1.0 — automated matches

CATH v4.4 (5 domains)

Domain ID domain_id5uioA00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology430 — Dihydrofolate Reductase, subunit A
Homologous superfamily homologous superfamily10 — Dihydrofolate Reductase, subunit A
Domain ID domain_id5uioB00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology430 — Dihydrofolate Reductase, subunit A
Homologous superfamily homologous superfamily10 — Dihydrofolate Reductase, subunit A
Domain ID domain_id5uioC00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology430 — Dihydrofolate Reductase, subunit A
Homologous superfamily homologous superfamily10 — Dihydrofolate Reductase, subunit A
Domain ID domain_id5uioD00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology430 — Dihydrofolate Reductase, subunit A
Homologous superfamily homologous superfamily10 — Dihydrofolate Reductase, subunit A
Domain ID domain_id5uioE00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology430 — Dihydrofolate Reductase, subunit A
Homologous superfamily homologous superfamily10 — Dihydrofolate Reductase, subunit A
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7. Citations (1)