SAXSdb — 5y7l

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

Calcium-activated potassium channel subunit beta-4

Homo sapiens

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	蛋白单体单体蛋白 × 1 PDB 声明：monomeric（1）与蛋白拷贝数一致	链 A； UniProt 45–166	片段：UNP RESIDUES 45-166	无其他共同聚合物	SOLUTION NMR NMR测量条件：pH 6.8；293 K；离子强度（mmCIF原始值）75；压力 1 NMR样品组成：0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O \| 90% H2O/10% D2O NMR样品组成：0.5 mM [U-13C; U-15N] Hbeta4, 25 mM sodium phosphate, 50 mM sodium chloride, 100% D2O \| 100% D2O	分辨率未提供

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 5Y7L	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
6V22 Cryo-EM structure of Ca2+-bound hsSlo1-beta4 channel complex 提交 2019-11-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 1–210（210 aa）链 F 1–210（210 aa）链 G 1–210（210 aa）链 H 1–210（210 aa）	未记录	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 60 CLR CHOLESTEROL × 16 K POTASSIUM ION × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE；Blot for 4 seconds before plunging.	分辨率 3.20 Å
6V35 Cryo-EM structure of Ca2+-free hsSlo1-beta4 channel complex 提交 2019-11-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 1–210（210 aa）链 F 1–210（210 aa）链 G 1–210（210 aa）链 H 1–210（210 aa）	未记录	PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 40 CLR CHOLESTEROL × 8 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8 cryo-EM玻璃化条件冷冻剂 ETHANE；Blot for 4 seconds before plunging.	分辨率 3.50 Å
9CZH Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in detergent. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 10	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.90 Å
9CZJ Ca2+ free hSlo1 + beta2N-beta4 channel in detergent. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	CLR CHOLESTEROL × 4 K POTASSIUM ION × 1	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.54 Å
9CZK Ca2+ free hSlo1 + beta2N-beta4 channel in nanodisc. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	K POTASSIUM ION × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 4 CLR CHOLESTEROL × 4	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 3.50 Å
9CZM Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in nanodisc. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）链 F 15–210（196 aa）链 G 15–210（196 aa）链 H 15–210（196 aa）	未记录	POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 32 MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 CLR CHOLESTEROL × 8 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.57 Å
9CZO Ca2+ bound intermediate state of hSlo1 + beta2N-beta4 channel in nanodisc. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	CA CALCIUM ION × 8 K POTASSIUM ION × 4 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 11	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.87 Å
9CZQ Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent. 提交 2024-08-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.88 Å
9D18 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 2 of inactivating domain 提交 2024-08-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.88 Å
9D19 Ca2+ bound open-inactivated hSlo1 + beta2N-beta4 channel in detergent-conformation 3 of inactivating domain 提交 2024-08-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 8 PDB 声明：octameric	链 E 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 F 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 G 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ; 链 H 15–210（196 aa）片段：;N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues),N-terminal 45 residues of kcnmb2 ligated to kcnmb4 (devoid of N terminal first 15 residues) ;	未记录	MG MAGNESIUM ION × 4 CA CALCIUM ION × 8 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 20 CLR CHOLESTEROL × 11 K POTASSIUM ION × 4	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 8；20 mM Tris-HCl pH 8.0, 450 mM KCl, 5 mM EDTA, 15 mM MgCl2, 0.02% GDN and 0.05 mg/ml POPE:POPC:POPA 5:5:1 (w:w:w). cryo-EM玻璃化条件冷冻剂 ETHANE	分辨率 2.88 Å

共 10 个其他 PDB 条目、10 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	KCMB4_HUMAN
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–122； UniProt 45–166

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	5y7l
沉积日期 deposition_date	2017-08-17
结构标题 title	Solution structure of Hbeta4 extracellular loop of BK potassium channel
关键词 keywords	Beta4, BK, METAL BINDING PROTEIN; METAL BINDING PROTEIN
实验方法 method	SOLUTION NMR

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	15.93 Å
回转半径 Rg (电子) rg_electron	15.87 Å
零角强度 I(0) i0	1300170000.00
分子量 molecular_weight	281890.0 kDa
排除体积 excluded_volume	342180 Å³
包络体积 envelope_volume	31887 Å³
水化壳体积 shell_volume	15750 Å³
包络直径 envelope_diameter	61.9 Å
壳层 Rg shell_rg	23.04 Å
包络 Rg envelope_rg	17.72 Å
形状 Rg shape_rg	15.85 Å
总 Rg total_rg	16.01 Å
总原子数 total_atoms	38020
残基数 n_residues	2440
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	53.4 Å
Rg (实空间) rg_real	15.93 Å
Rg 误差 (实空间) rg_real_error	0.33 Å
I(0) (实空间) i0_real	1.3000e+09
I(0) 误差 (实空间) i0_real_error	1.3920e+07
Rg (倒空间) rg_reciprocal	15.93 Å
I(0) (倒空间) i0_reciprocal	1300000000.0000
解质量估计 total_estimate	0.8852
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	18.9 Å
偏度 Skewness skewness	0.318
峰度 Kurtosis kurtosis	-0.279
角度范围 angular_range	— – 0.5000 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	286200.0000
实空间数据点数 n_real_points	80
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.856; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.968; Smooth: 0.968

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (1)

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8. 引用文献 (1)

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描述 description	Calcium-activated potassium channel subunit beta-4
分子量 formula_weight	14114.6 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合物类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	38020

来源生物 organism	Homo sapiens
通用名 common_name	Human
NCBI 分类编号 ncbi_taxonomy_id	9606
宿主生物 host_organism	Escherichia coli

fit	/app/data/saxs_computation/y7/5y7l/5y7l.fit
pddf	/app/data/pr_computation/y7/5y7l/5y7l.pr.out
profile	/app/data/saxs_computation/y7/5y7l/5y7l.dat

Solution structure of Hbeta4 extracellular loop of BK potassium channel

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Calcium-activated potassium channel subunit beta-4

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (1)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)