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6c0a

Actinin-1 EF-Hand bound to the Cav1.2 IQ Motif

Method: SOLUTION NMR Dmax: 47.3 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Alpha-actinin-1

Mus musculus

UniProt Q7TPR4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 822–892 Fragment：EF-hand 3,4 (UNP residues 822-892) Voltage-dependent L-type calcium channel subunit alpha-1C × 1 （Q13936） SOLUTION NMR NMR measurement conditions：pH 7.5；303 K；Ionic strength (raw mmCIF value) 0；Pressure 1 NMR sample composition：300 uM [U-99% 15N] Actinin-1 EF hand, 300 uM IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,3, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：300 uM [U-99% 13C; U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,4, 90% H2O/10% D2O | 90% H2O/10% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name ACTN1_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–71； UniProt 822–892

Voltage-dependent L-type calcium channel subunit alpha-1C

OrganismNot specified

UniProt Q13936

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain B； UniProt 1587–1609 Fragment：IQ Motif (UNP residues 1587-1609) Alpha-actinin-1 × 1 （Q7TPR4） SOLUTION NMR NMR measurement conditions：pH 7.5；303 K；Ionic strength (raw mmCIF value) 0；Pressure 1 NMR sample composition：300 uM [U-99% 15N] Actinin-1 EF hand, 300 uM IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,3, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：300 uM [U-99% 13C; U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O | 90% H2O/10% D2O NMR sample composition：12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,4, 90% H2O/10% D2O | 90% H2O/10% D2O Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6C0A Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1T0J Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit Deposited 2004-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 428–445（18 aa） Fragment：Residues 428-443
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；Tris-Cl, PEG 4000, NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.242
1T0J Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and a peptide of the alpha1c subunit Deposited 2004-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 428–445（18 aa） Fragment：Residues 428-443
Not recorded CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；Tris-Cl, PEG 4000, NaCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.00 Å R-free 0.242
2LQC NMR solution structure of a Ca2+-Calmodulin with a binding motif (NSCaTE) peptide from the N-terminal cytoplasmic domain of the L-type Voltage-Cated Calcium Channel alpha1C subunit Deposited 2012-02-29 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 47–68（22 aa） Fragment：UNP residues 47-68
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7；298 K；Ionic strength (raw mmCIF value) 100；Pressure ambient
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] calmodulin, 2 mM NSCaTE peptide, 55 M H2O, 5 M [U-99% 2H] D2O, 10 mM DTT, 20 mM TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] NSCaTE peptide, 0.5 mM calmodulin, 55 M H2O, 5 M [U-99% 2H] D2O, 10 mM DTT, 20 mM TRIS, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] calmodulin, 2 mM NSCaTE peptide, 7.5 mg Pf1 phage, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
3G43 Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer Deposited 2009-02-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1609–1682（74 aa） Fragment：C-terminal fragment: UNP residues 1609-1682
Chain F 1609–1682（74 aa） Fragment：C-terminal fragment: UNP residues 1609-1682
Not recorded CA CALCIUM ION × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；20% PEG 6000, 1.0 M LiCl, 3% w/v MPD, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.10 Å R-free 0.269
3OXQ Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex Deposited 2010-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain E 1609–1685（77 aa） Fragment：preIQ/IQ domain (unp residues 1609-1685)
Not recorded CA CALCIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M Li2SO4, 24.5% PEG3350, 0.1 M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.55 Å R-free 0.288
3OXQ Crystal Structure of Ca2+/CaM-CaV1.2 pre-IQ/IQ domain complex Deposited 2010-09-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain F 1609–1685（77 aa） Fragment：preIQ/IQ domain (unp residues 1609-1685)
Not recorded CA CALCIUM ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；0.2 M Li2SO4, 24.5% PEG3350, 0.1 M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.55 Å R-free 0.288
5V2P CaV beta2a subunit: CaV1.2 AID-CAP complex Deposited 2017-03-06 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 427–445（19 aa） Fragment：AID-CAP (UNP residues 427-445)
Mutation：K427C, Q428S, Q429P, E432C NI NICKEL (II) ION × 1 1PE PENTAETHYLENE GLYCOL × 2 8VY 1,3-bis(bromomethyl)benzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；34-37% PEG400, 0.1 M magnesium chloride, 0.1 M MES, pH 6.3
Resolution 2.00 Å R-free 0.230
5V2Q CaV beta2a subunit: CaV1.2 AID-CEN complex Deposited 2017-03-06 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 427–445（19 aa） Fragment：AID-CEN (UNP residues 427-445)
Mutation：K427A, Q428S, Q429P, K435C, D439C CL CHLORIDE ION × 1 8VY 1,3-bis(bromomethyl)benzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；1.5-1.7 M ammonium sulfate, 5 mM BME, 0.1 M HEPES, pH 7.0
Resolution 1.70 Å R-free 0.196
6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
Resolution 1.65 Å R-free 0.208
6DAD 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；2.1 M sodium malonate, 0.1 M HEPES, pH 7.5
Resolution 1.65 Å R-free 0.208
6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
Resolution 2.00 Å R-free 0.226
6DAE 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 10.3；277 K；1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3
Resolution 2.00 Å R-free 0.226
6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
Resolution 2.40 Å R-free 0.243
6DAF 2.4 Angstrom crystal structure of the F141L Ca/CaM:CaV1.2 IQ domain complex Deposited 2018-05-01 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa） Fragment：UNP residues 1611-1644
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；0.5 M potassium nitrate, 20% PEG3350
Resolution 2.40 Å R-free 0.243
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 10 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain T 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 5 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 6 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain L 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 7 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain N 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 8 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain P 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U39 2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 9 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain R 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；ammonium sulfate
Resolution 2.40 Å R-free 0.283
6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 3 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
Resolution 1.65 Å R-free 0.209
6U3A 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；277 K；DL-malic acid
Resolution 1.65 Å R-free 0.209
6U3B 1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 550 MME, sodium acetate
Resolution 1.70 Å R-free 0.200
6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
Resolution 1.75 Å R-free 0.201
6U3D 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex Deposited 2019-08-21 Different construct Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 1611–1644（34 aa）
Not recorded CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG 3350, citric acid
Resolution 1.75 Å R-free 0.201
7L8V NMR Structure of half-calcified calmodulin mutant (CaMEF12) bound to the IQ-motif of CaV1.2 Deposited 2021-01-01 Different construct Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1662–1682（21 aa） Fragment：IQ-motif residues 1662-1682
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7.4；303 K；Ionic strength (raw mmCIF value) 0.1；Pressure 1
NMR sample composition 0.5 mM [U-95% 15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.5 mM [U-13C; U-15N] calmodulin, 0.75 mM IQ-motif, 10 mM TRIS, 2 mM CALCIUM ION, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
8EOI Structure of a human EMC:human Cav1.2 channel complex in GDN detergent Deposited 2022-10-03 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 11 PDB declaration: undecameric
Chain K 109–1658（1550 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8FD7 Structure of the human L-type voltage-gated calcium channel Cav1.2 complexed with gabapentin Deposited 2022-12-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain K 1–1648（1648 aa）
Not recorded NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 GBN [1-(AMINOMETHYL)CYCLOHEXYL]ACETIC ACID × 1 CA CALCIUM ION × 3 NA SODIUM ION × 1 WO9 (2R)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl heptadecanoate × 1 YSW (2S)-3-{[(R)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-2-(dodecanoyloxy)propyl dodecanoate × 1 CLR CHOLESTEROL × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8FHS Human L-type voltage-gated calcium channel Cav1.2 in the presence of amiodarone and sofosbuvir at 3.3 Angstrom resolution Deposited 2022-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2221（2221 aa）
Not recorded CA CALCIUM ION × 2 BBI (2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methanone × 1 WG6 Sofosbuvir × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 2 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8WE6 Human L-type voltage-gated calcium channel Cav1.2 at 2.9 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 CLR CHOLESTEROL × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8WE7 Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine at 3.2 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 CLR CHOLESTEROL × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8WE8 Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine, amlodipine and pinaverium at 2.9 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 CLR CHOLESTEROL × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 6UB amlodipine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8WE9 Human L-type voltage-gated calcium channel Cav1.2 (Class I) in the presence of pinaverium at 3.0 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 CLR CHOLESTEROL × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8WEA Human L-type voltage-gated calcium channel Cav1.2 (Class II) in the presence of pinaverium at 3.2 Angstrom resolution Deposited 2023-09-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–2201（2201 aa）
Not recorded CA CALCIUM ION × 2 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 WB9 4-[(2-bromanyl-4,5-dimethoxy-phenyl)methyl]-4-[2-[2-[(1~{R},2~{S},5~{R})-6,6-dimethyl-2-bicyclo[3.1.1]heptanyl]ethoxy]ethyl]morpholin-4-ium × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9ZZ4 NMR structure of CaBP1 bound to the IQ motif of Cav1.2 Deposited 2026-01-06 Different ligand/ion Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1587–1609（23 aa） Fragment：IQ Motif (UNP residues 1587-1609)
Not recorded CA CALCIUM ION × 2 SOLUTION NMR
NMR measurement conditions pH 7.5；300 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition 0.5 mM [U-100% 15N] CaBP1 C-Lobe, 0.75 mM CaV1.2 IQ motif, 1 mM Ca2+, 10 mM [U-100% 2H] Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 0.6 mM [U-100% 13C; U-100% 15N] CaBP1 C-Lobe, 0.9 mM CaV1.2 IQ motif, 1 mM Ca2+, 10 mM [U-100% 2H] Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided

23 other PDB entries and 39 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CAC1C_HUMAN
Isoform Q13936-17
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–23； UniProt 1587–1609

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6c0a

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6c0a
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6c0a
Deposition date deposition_date2017-12-28
Structure title titleActinin-1 EF-Hand bound to the Cav1.2 IQ Motif
Keywords keywords;actinin-1, EF hand, Calcium Calmodulin, Neuronal signaling, Voltage Gated Calcium Channel, Cav1.2, Voltage Gated Calcium Channel 1.2, Hippocampal Neurons, IQ Motif, SIGNALING PROTEIN ;; SIGNALING PROTEIN
Experimental Method methodSOLUTION NMR
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier14.13 Å
Radius of gyration Rg (electron density) rg_electron13.53 Å
Forward intensity I(0) i028306200.00
Molecular weight molecular_weight43017.0 kDa
Excluded volume excluded_volume53781 ų
Envelope volume envelope_volume18457 ų
Hydration-shell volume shell_volume11530 ų
Envelope diameter envelope_diameter49.6 Å
Shell Rg shell_rg19.38 Å
Envelope Rg envelope_rg14.28 Å
Shape Rg shape_rg13.53 Å
Total Rg total_rg13.99 Å
Total atoms total_atoms5952
Residues n_residues376
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax47.3 Å
Rg (real space) rg_real14.10 Å
Rg uncertainty (real space) rg_real_error0.38 Å
I(0) (real space) i0_real2.8310e+07
I(0) uncertainty (real space) i0_real_error3.4750e+05
Rg (reciprocal space) rg_reciprocal14.11 Å
I(0) (reciprocal space) i0_reciprocal28310000.0000
Solution quality estimate total_estimate0.7731
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks3
Primary peak position r_peak_primary17.4 Å
Skewness Skewness skewness0.286
Kurtosis Kurtosis kurtosis-0.114
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha151700.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.682; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id6c0aA00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
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8. Citations (2)

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9. Files and Curves (10)