6d6v

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Telomerase-associated protein 82

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 6 DNA 1 RNA 1	Telomerase reverse transcriptase × 1 （O77448） Telomerase holoenzyme TEB heterotrimer Teb3 subunit × 1 （A0A0U8UFF4） Telomerase holoenzyme Teb2 subunit × 1 （A0A0U8TRG9） RNA (159-MER) × 1 Telomerase-associated protein 50 × 1 Telomerase La-related protein p65 × 1 （W7X6T2） ;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ; × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	D2CVN6_TETTH
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain D； PDBConstruct 1–701； UniProt 1–701

Telomerase reverse transcriptase

OrganismNot specified

UniProt O77448

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 6 DNA 1 RNA 1	Telomerase-associated protein 82 × 1 （D2CVN6） Telomerase holoenzyme TEB heterotrimer Teb3 subunit × 1 （A0A0U8UFF4） Telomerase holoenzyme Teb2 subunit × 1 （A0A0U8TRG9） RNA (159-MER) × 1 Telomerase-associated protein 50 × 1 Telomerase La-related protein p65 × 1 （W7X6T2） ;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ; × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TERT_TETTH
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain A； PDBConstruct 1–1117； UniProt 1–1117

Telomerase holoenzyme TEB heterotrimer Teb3 subunit

OrganismNot specified

UniProt A0A0U8UFF4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 6 DNA 1 RNA 1	Telomerase-associated protein 82 × 1 （D2CVN6） Telomerase reverse transcriptase × 1 （O77448） Telomerase holoenzyme Teb2 subunit × 1 （A0A0U8TRG9） RNA (159-MER) × 1 Telomerase-associated protein 50 × 1 Telomerase La-related protein p65 × 1 （W7X6T2） ;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ; × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U8UFF4_TETTH
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain F； PDBConstruct 1–121； UniProt 1–121

Telomerase holoenzyme Teb2 subunit

OrganismNot specified

UniProt A0A0U8TRG9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 6 DNA 1 RNA 1	Telomerase-associated protein 82 × 1 （D2CVN6） Telomerase reverse transcriptase × 1 （O77448） Telomerase holoenzyme TEB heterotrimer Teb3 subunit × 1 （A0A0U8UFF4） RNA (159-MER) × 1 Telomerase-associated protein 50 × 1 Telomerase La-related protein p65 × 1 （W7X6T2） ;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ; × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A0U8TRG9_TETTH
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain E； PDBConstruct 1–269； UniProt 1–269

Telomerase La-related protein p65

OrganismNot specified

UniProt W7X6T2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Other combination	Heteromer	Protein 6 DNA 1 RNA 1	Telomerase-associated protein 82 × 1 （D2CVN6） Telomerase reverse transcriptase × 1 （O77448） Telomerase holoenzyme TEB heterotrimer Teb3 subunit × 1 （A0A0U8UFF4） Telomerase holoenzyme Teb2 subunit × 1 （A0A0U8TRG9） RNA (159-MER) × 1 Telomerase-associated protein 50 × 1 ;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ; × 1 ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	LARP7_TETTS
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain H； PDBConstruct 1–542； UniProt 1–542

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6d6v
Deposition date deposition_date	2018-04-22
Structure title title	CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution
Keywords keywords	Telomerase, telomere, REPLICATION; REPLICATION
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6d6v__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6d6v__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6d6v__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	46.21 Å
Rg (electron density)	45.31 Å
Total Rg	45.59 Å
Atom count	17249
Residues	1868
Excluded volume	294920 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6d6v__assembly_1__model_1`	octameric （8）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Description description	Telomerase-associated protein 82
Molecular weight formula_weight	82040.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D
Atom count atom_count	17249

MKLTKGGSYILKKVDRKQFYQDEEIVMQIKKILGQKTTDCKQYIKCECIDGLGDEALIYFEMLANQNQHLQKNDVIMIQDYLNDKTQNDKIVVLVTRFQFCKASHVQPKTAQKESIQLLNTEKTIIQKSKITKNPAEEVLKFIEVNEKDNSSNSEDMIIEQQKQEIKNNQKEKQSINGFNLEDSYSNISDITNFGGKSNFNIGSLSDQLSKQTLLISQLQVGKNRFSFKFEGRVVYKSSTFQNQQDSKYFFITAQDANNQEINLSFWQKVDQSYQTLKVGQYYYFIGGEVKQFKNNLELKFKFGDYQIIPKETLSANYVQPLALQPSKQFGNDSIGDSDYSIHNLIEKEESIAQKGYNGQKNNKYRQNNNNSKHTLLISEVLKTSKQYLSVLAQVVDIQSSDKNIRLKICDNSCNQELKVVIFPDLCYEWRDKFSINKWYYFNEFVRQIYNDEVQLKNNIHSSIKESDDQRKVITYNQEQGVFKKSISINSNDSFEIKPKISYKNNSNQEQRIYSSIEEIIQQAQASEIGQKKEFYVYGNLVSIQMKNKLYYYRCTCQGKSVLKYHGDSFFCESCQQFINPQVHLMLRAFVQDSTGTIPVMIFDQQSSQLINQIDPSIHVQEAGQYVKNCIENGQEEIIRQLFSKLDFARFIFEIQFENKEFNNEQEIAYKVLKIEKENIKEESKYLLKKLEHLINNNQNN

Entity 2 Entity 2 polymer

Description description	Telomerase reverse transcriptase
Molecular weight formula_weight	133486.9 kDa
EC 编号 ec_number	2.7.7.49
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MQKINNINNNKQMLTRKEDLLTVLKQISALKYVSNLYEFLLATEKIVQTSELDTQFQEFLTTTIIASEQNLVENYKQKYNQPNFSQLTIKQVIDDSIILLGNKQNYVQQIGTTTIGFYVEYENINLSRQTLYSSNFRNLLNIFGEEDFKYFLIDFLVFTKVEQNGYLQVAGVCLNQYFSVQVKQKKWYKNNFNMNGKATSNNNQNNANLSNEKKQENQYIYPEIQRSQIFYCNHMGREPGVFKSSFFNYSEIKKGFQFKVIQEKLQGRQFINSDKIKPDHPQTIIKKTLLKEYQSKNFSCQEERDLFLEFTEKIVQNFHNINFNYLLKKFCKLPENYQSLKSQVKQIVQSENKANQQSCENLFNSLYDTEISYKQITNFLRQIIQNCVPNQLLGKKNFKVFLEKLYEFVQMKRFENQKVLDYICFMDVFDVEWFVDLKNQKFTQKRKYISDKRKILGDLIVFIINKIVIPVLRYNFYITEKHKEGSQIFYYRKPIWKLVSKLTIVKLEEENLEKVEEKLIPEDSFQKYPQGKLRIIPKKGSFRPIMTFLRKDKQKNIKLNLNQILMDSQLVFRNLKDMLGQKIGYSVFDNKQISEKFAQFIEKWKNKGRPQLYYVTLDIKKCYDSIDQMKLLNFFNQSDLIQDTYFINKYLLFQRNKRPLLQIQQTNNLNSAMEIEEEKINKKPFKMDNINFPYYFNLKERQIAYSLYDDDDQILQKGFKEIQSDDRPFIVINQDKPRCITKDIIHNHLKHISQYNVISFNKVKFRQKRGIPQGLNISGVLCSFYFGKLEEEYTQFLKNAEQVNGSINLLMRLTDDYLFISDSQQNALNLIVQLQNCANNNGFMFNDQKITTNFQFPQEDYNLEHFKISVQNECQWIGKSIDMNTLEIKSIQKQTQQEINQTINVAISIKNLKSQLKNKLRSLFLNQLIDYFNPNINSFEGLCRQLYHHSKATVMKFYPFMTKLFQIDLKKSKQYSVQYGKENTNENFLKDILYYTVEDVCKILCYLQFEDEINSNIKEIFKNLYSWIMWDIIVSYLKKKKQFKGYLNKLLQKIRKSRFFYLKEGCKSLQLILSQQKYQLNKKELEAIEFIDLNNLIQDIKTLIPKISAKSNQQNTN

Entity 3 Entity 3 polymer

Description description	Telomerase holoenzyme TEB heterotrimer Teb3 subunit
Molecular weight formula_weight	14007.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MDAEQEQVMYPRILFEQMAQFRGKKVTVVGNVCNEDQNDSLVIEFGPTGLNQHVVIDNYRRVDLNNTTKFVEIRGVVLNQNIVSCEELTEFEQKDPFDFDTYSKLIHLSQSDKLSSLFTDQ

Entity 4 Entity 4 polymer

Description description	Telomerase holoenzyme Teb2 subunit
Molecular weight formula_weight	30993.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MSNRVQGGFDNNSGNNQSAQKQQAEKIPQITVPLNCFMINQIVKAAKENPQAHSGNHYEWYGAFENAIITAKFEFLQSINDSPKIMGKLSDSTGCIEVVIQKSKMSDELPEFVQAYEIELQNNGNRHKYVRAMLKMRKNAQIQLLYFSIVNDANEISRHGLDLCLRYLQRKHGIEDFMHMTNDKAHNNHNASAQKVHYQIDRNQQPKEQVLELMRQILKHNPNDQIPKSKIIEFFQSQLNQVQINQILQQLVSANEIFSVGSDNYLLNV

Entity 5 Entity 5 polymer

Description description	RNA (159-MER)
Molecular weight formula_weight	50747.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	B

AUACCCGCUUAAUUCAUUCAGAUCUGUAAUAGAACUGUCAUUCAACCCCAAAAAUCUAGUGCUGAUAUAACCUUCACCAAUUAGGUUCAAAUAAGUGGUAAUGCGGGACAAAAGACUAUCGACAUUUGAUACACUAUUUAUCAAUGGAUGUCUUAUUUU

Entity 6 Entity 6 polymer

Description description	Telomerase-associated protein 50
Molecular weight formula_weight	13379.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 7 Entity 7 polymer

Description description	Telomerase La-related protein p65
Molecular weight formula_weight	64207.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H

MDEYLENTNLEELEQECFMEDYQHEDVVEQENHQVDANDIYENQQMNDESQLNQDVKISQQKEQAVEMIEEQQQNNQDKFKQFQDCMAHITELNFKRNYQNLTEQSSSNNVVAEELDIKESLKLQMEYYFCDTNLTHDSYLRGIISKSPKNCVDIKVFLKFNKIQQILKQIQDKQIVSTYGIENQSQKKNHKNYKNQNATFSKKDLIHLIRDSLKESKILKVKMDSLKVKRRFPFNLEQALKNSKQRTLYIDFLPPKCSKQTLVSIFGNFRIININLPLQKNSQLCQGFAFIEFFSEEEANQALITKNSSIPKELILLTEKKIGQGSIRIITYKKWQEEKQSFKELSKNQNEQKNKNMNQSRKASDEFVSIDVEIKQNCLIKIINIPQGTLKAEVVLAVRHLGYEFYCDYIDENSNQINSNKISLSTQQQNTAQCSNIQIENNLIQQDQHPQLNDLLKEGQAMIRFQNSDEQRLAIQKLLNHNNNKLQIEIRGQICDVISTIPEDEEKNYWNYIKFKKNEFRKFFFMKKQQKKQNITQNYNK

Entity 8 Entity 8 polymer

Description description	;DNA (5'-D(PGPTPTPGPGPGPGPTPTPGPGPGPGPTPTPGPGPGP*G)-3') ;
Molecular weight formula_weight	6059.9 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polydeoxyribonucleotide
Chain IDs strand_id	C

GTTGGGGTTGGGGTTGGGG

Entity 9 Entity 9 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.800 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	4.80 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	52506

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

1. Protein Identity and Related Structures Protein Identity & Related Structures

Telomerase-associated protein 82

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Telomerase reverse transcriptase

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Telomerase holoenzyme TEB heterotrimer Teb3 subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Telomerase holoenzyme Teb2 subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Telomerase La-related protein p65

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

6d6v__assembly_1__model_1

6d6v__assembly_1__model_1 | I(q)

6d6v__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

冷冻电镜 EM 3D

5. Entities and Polymers Entities & Polymers (9)

Entity 1 Entity 1 polymer

Polymer Polymer

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 polymer

Polymer Polymer

Entity 4 Entity 4 polymer

Polymer Polymer

Entity 5 Entity 5 polymer

Polymer Polymer

Entity 6 Entity 6 polymer

Polymer Polymer

Entity 7 Entity 7 polymer

Polymer Polymer

Entity 8 Entity 8 polymer

Polymer Polymer

Entity 9 Entity 9 non-polymer

7. Citations (1)