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6vzk

Crystal structure of human CaMKII-alpha (CAMK2A)kinase domain

Method: X-RAY DIFFRACTION Dmax: 65.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Calcium/calmodulin-dependent protein kinase type II subunit alpha

Homo sapiens

UniProt Q9UQM7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 7–274 Mutation：D135N, Q223K HC4 4'-HYDROXYCINNAMIC ACID × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；PEG 6000, 50 mM HEPES, 3-Aminobenzene-sulfonic acid, 5-Sulfosalicylic acid dihydrate, p-Coumaric acid, PIPES, Terephthalic acid, Vanillic acid Resolution 2.55 Å R-free 0.294

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 6VZK Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2VZ6 Structure of human calcium calmodulin dependent protein kinase type II alpha (CAMK2A) in complex with Indirubin E804 Deposited 2008-07-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 13–302（290 aa） Fragment：KINASE DOMAIN, RESIDUES 13-302
Not recorded FEF (2Z,3E)-2,3'-BIINDOLE-2',3(1H,1'H)-DIONE 3-{O-[(3R)-3,4-DIHYDROXYBUTYL]OXIME} × 1 PGO S-1,2-PROPANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；12% PEG6000, 0.1M TRIS PH8.5
Resolution 2.30 Å R-free 0.203
2VZ6 Structure of human calcium calmodulin dependent protein kinase type II alpha (CAMK2A) in complex with Indirubin E804 Deposited 2008-07-30 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 13–302（290 aa） Fragment：KINASE DOMAIN, RESIDUES 13-302
Not recorded FEF (2Z,3E)-2,3'-BIINDOLE-2',3(1H,1'H)-DIONE 3-{O-[(3R)-3,4-DIHYDROXYBUTYL]OXIME} × 1 PGO S-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.5；12% PEG6000, 0.1M TRIS PH8.5
Resolution 2.30 Å R-free 0.203
3SOA Full-length human CaMKII Deposited 2011-06-30 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 1–474（474 aa）
Mutation：K42M, D135N, T306V DB8 4-[(2,4-dichloro-5-methoxyphenyl)amino]-6-methoxy-7-[3-(4-methylpiperazin-1-yl)propoxy]quinoline-3-carbonitrile × 12 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 5.5；288.15 K；1.2 M Ammonium Tartrate, 100 mM bistrispropane, pH 5.5, VAPOR DIFFUSION, temperature 288.15K
Resolution 3.55 Å R-free 0.327
5IG3 Crystal structure of the human CaMKII-alpha hub Deposited 2016-02-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 12 PDB declaration: dodecameric
Chain A 345–475（131 aa） Fragment：UNP residues 345-475
Chain B 345–475（131 aa） Fragment：UNP residues 345-475
Chain C 345–475（131 aa） Fragment：UNP residues 345-475
Chain D 345–475（131 aa） Fragment：UNP residues 345-475
Chain E 345–475（131 aa） Fragment：UNP residues 345-475
Chain F 345–475（131 aa） Fragment：UNP residues 345-475
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.3；295 K；35 % (v/v) MPD, 0.1 M HEPES pH 7.3
Resolution 2.75 Å R-free 0.268
6OF8 Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CamKII-alpha hub domain Deposited 2019-03-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric
Chain A 345–475（131 aa）
Chain B 345–475（131 aa）
Chain C 345–475（131 aa）
Chain D 345–475（131 aa）
Chain E 345–475（131 aa）
Chain F 345–475（131 aa）
Chain G 345–475（131 aa）
Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M K POTASSIUM ION × 10 GOL GLYCEROL × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；17 mg/mL mutant hub in 25 mM Tris, 150 mM KCl, 10% (v/v) glycerol, 2 mM DTT, 1 mM TCEP, pH 8.0 was mixed 1:1 with 235 mM K3Citrate, 15% (w/v) PEG 3350, pH 8.0
Resolution 2.10 Å R-free 0.258
6W4O CaMKII alpha-30 Cryo-EM reconstruction Deposited 2020-03-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 13 PDB declaration: tridecameric
Chain A 7–478（472 aa）
Chain B 7–478（472 aa）
Chain C 7–478（472 aa）
Chain D 7–478（472 aa）
Chain E 7–478（472 aa）
Chain F 7–478（472 aa）
Chain G 7–478（472 aa）
Chain I 7–478（472 aa）
Chain J 7–478（472 aa）
Chain K 7–478（472 aa）
Chain L 7–478（472 aa）
Chain M 7–478（472 aa）
Chain O 7–478（472 aa）
Mutation：K37M No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6W4P CaMKII alpha-30 Cryo-EM reconstruction - Class B Deposited 2020-03-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 13 PDB declaration: tridecameric
Chain A 7–478（472 aa）
Chain B 7–478（472 aa）
Chain C 7–478（472 aa）
Chain D 7–478（472 aa）
Chain E 7–478（472 aa）
Chain F 7–478（472 aa）
Chain G 7–478（472 aa）
Chain H 7–478（472 aa）
Chain I 7–478（472 aa）
Chain J 7–478（472 aa）
Chain K 7–478（472 aa）
Chain L 7–478（472 aa）
Chain M 7–478（472 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.60 Å
6X5G Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and LRRC7 inhibitory domain Deposited 2020-05-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K GOL GLYCEROL × 2 BCN BICINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；277 K；10% w/v PEG 20000, 2% v/v 1,4-Dioxane, 0.1 M Bicine
Resolution 1.85 Å R-free 0.227
6X5Q Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluA1 Deposited 2020-05-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K GOL GLYCEROL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；0.1 M Tris, 28% w/v PEG 4000
Resolution 2.14 Å R-free 0.235
7KL0 Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2020-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K UZD methyl 6-O-(heptylcarbamoyl)-beta-L-altropyranoside × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 6000, 19mM HECAMEG
Resolution 2.40 Å R-free 0.258
7KL0 Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2020-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K UZD methyl 6-O-(heptylcarbamoyl)-beta-L-altropyranoside × 1 EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 6000, 19mM HECAMEG
Resolution 2.40 Å R-free 0.258
7KL1 Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2020-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K EDO 1,2-ETHANEDIOL × 1 MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 6000, 19mM HECAMEG
Resolution 2.40 Å R-free 0.258
7KL1 Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2020-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K EDO 1,2-ETHANEDIOL × 2 MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 6000, 19mM HECAMEG
Resolution 2.40 Å R-free 0.258
7KL2 Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2020-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.15 M DL-Malic acid pH 7, 20% PEG 3350
Resolution 2.56 Å R-free 0.293
7REC Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CaMKII alpha hub bound to 5-HDC Deposited 2021-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric
Chain A 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain B 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain C 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain D 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain E 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain F 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Chain G 345–475（131 aa） Fragment：hub (UNP residues 345-475)
Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M Mutation：T354N, E355Q, T412N, I414M, I464H, F467M 7ZV 5-hydroxydiclofenac × 12 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.1；298 K；100 nL protein + compound in 25 mM Tris, 150 mM potassium chloride, 2 mM DTT, 1 mM TCEP, 10% v/v glycerol, pH 8.0, 10% DMSO + 100 nL well solution (200 mM potassium acetate, 20% w/v PEG3350, pH 8.1), equilibrated against 45 uL well solution
Resolution 2.20 Å R-free 0.244
7UIQ Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and Tiam1 Deposited 2022-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, 16% PEG 6000, 0.1% v/v Triton X-114
Resolution 3.11 Å R-free 0.309
7UIQ Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and Tiam1 Deposited 2022-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, 16% PEG 6000, 0.1% v/v Triton X-114
Resolution 3.11 Å R-free 0.309
7UIR Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and Tiam1 in complex with ATP Deposited 2022-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, 16% PEG 6000, 0.1% v/v Triton X-114
Resolution 3.10 Å R-free 0.274
7UIR Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and Tiam1 in complex with ATP Deposited 2022-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M HEPES, 16% PEG 6000, 0.1% v/v Triton X-114
Resolution 3.10 Å R-free 0.274
7UIS Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) Deposited 2022-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M Bis-tris propane pH 6.5, 0.2 M Sodium chloride, 25% PEG 3350
Resolution 2.58 Å R-free 0.268
7UJP Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B Deposited 2022-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；277 K；0.1 M Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 0.1 M Ammonium sulfate, 25% PEG 3350, 19mM Methyl 6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside
Resolution 2.56 Å R-free 0.281
7UJP Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B Deposited 2022-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；277 K；0.1 M Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 0.1 M Ammonium sulfate, 25% PEG 3350, 19mM Methyl 6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside
Resolution 2.56 Å R-free 0.281
7UJQ Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B Deposited 2022-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K UZD methyl 6-O-(heptylcarbamoyl)-beta-L-altropyranoside × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；277 K；0.1 M Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 0.1 M Ammonium sulfate, 25% PEG 3350, 19mM Methyl 6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside
Resolution 2.25 Å R-free 0.271
7UJQ Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B Deposited 2022-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 7–274（268 aa）
Mutation：D135N, Q223K UZD methyl 6-O-(heptylcarbamoyl)-beta-L-altropyranoside × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；277 K；0.1 M Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, 0.1 M Ammonium sulfate, 25% PEG 3350, 19mM Methyl 6-O-(N-heptylcarbamoyl)-alpha-D-glucopyranoside
Resolution 2.25 Å R-free 0.271
7UJR Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B Deposited 2022-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：Q223K EDO 1,2-ETHANEDIOL × 3 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.2；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 3350, 5% Ethanol
Resolution 1.95 Å R-free 0.252
7UJS Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B in complex with ADP Deposited 2022-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：Q223K ADP ADENOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.1 M Ammonium sulfate, 20% PEG 3350
Resolution 2.75 Å R-free 0.262
7UJT Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D) in complex with ATP Deposited 2022-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 7–274（268 aa）
Mutation：D135N, Q223K ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GOL GLYCEROL × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.1M 1,3-bis(tris(hydroxymethyl)methylamino)propane, 0.2 M Sodium chloride, 25% PEG 3350
Resolution 2.10 Å R-free 0.246
9EOY Structure of Thr354Asn, Glu355Gln, Thr412Asn, Ile414Met, Ile464His, and Phe467Met mutant human CaMKII alpha hub bound to PIPA Deposited 2024-03-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric
Chain A 345–475（131 aa）
Chain B 345–475（131 aa）
Chain C 345–475（131 aa）
Chain D 345–475（131 aa）
Chain E 345–475（131 aa）
Chain F 345–475（131 aa）
Chain G 345–475（131 aa）
Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M Mutation：T354N, E355Q, T412N, I414M, I464H, and F467M ACT ACETATE ION × 12 A1H6K 2-[6-(4-chlorophenyl)imidazo[1,2-b]pyridazin-2-yl]ethanoic acid × 4 PG4 TETRAETHYLENE GLYCOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293.15 K；500 nL protein + 2.65 mM compound in 25 mM Tris, 150 mM KCl, 10 mM imidazole, 1 mM DTT, 10% glycerol, pH 8.5, 10% DMSO + 1000 nL well solution (11% w/v PEG3350, 300 mM potassium acetate, pH 8.0), equilibrated against 50 uL well solution.
Resolution 2.10 Å R-free 0.251

21 other PDB entries and 28 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCC2A_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–268； UniProt 7–274

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 6vzk

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 6vzk
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2. Structure Basics 2. Structure Basics

Entry ID entry_id6vzk
Deposition date deposition_date2020-02-28
Structure title titleCrystal structure of human CaMKII-alpha (CAMK2A)kinase domain
Keywords keywordsCaMKII, Kinase, Human, CAMK2A, TRANSFERASE; TRANSFERASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier20.20 Å
Radius of gyration Rg (electron density) rg_electron19.08 Å
Forward intensity I(0) i014619800.00
Molecular weight molecular_weight29502.0 kDa
Excluded volume excluded_volume37221 ų
Envelope volume envelope_volume43103 ų
Hydration-shell volume shell_volume19184 ų
Envelope diameter envelope_diameter67.2 Å
Shell Rg shell_rg25.16 Å
Envelope Rg envelope_rg19.28 Å
Shape Rg shape_rg19.10 Å
Total Rg total_rg19.93 Å
Total atoms total_atoms2087
Residues n_residues258
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax65.2 Å
Rg (real space) rg_real20.15 Å
Rg uncertainty (real space) rg_real_error0.32 Å
I(0) (real space) i0_real1.4620e+07
I(0) uncertainty (real space) i0_real_error1.8960e+05
Rg (reciprocal space) rg_reciprocal20.16 Å
I(0) (reciprocal space) i0_reciprocal14620000.0000
Solution quality estimate total_estimate0.8963
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary24.2 Å
Skewness Skewness skewness0.278
Kurtosis Kurtosis kurtosis-0.372
Angular range angular_range— – 0.3950 Å−1
Current regularization parameter α current_alpha0.0001
Highest regularization parameter α highest_alpha4273000.0000
Real-space data points n_real_points71
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.889; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.980
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_idd6vzka_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.144 — Protein kinase-like (PK-like)
Superfamily Superfamily superfamilyd.144.1 — Protein kinase-like (PK-like)
Family Family familyd.144.1.0 — automated matches

CATH v4.4 (1 domains)

Domain ID domain_id6vzkA01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology200 — Phosphorylase Kinase; domain 1
Homologous superfamily homologous superfamily20 — Phosphorylase Kinase; domain 1
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8. Citations (1)

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9. Files and Curves (10)