Stromal interaction molecule 1
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 234–343 | Not recorded | No other associated polymer | SOLUTION NMR NMR measurement conditions：pH 7.25；310.15 K；Ionic strength (raw mmCIF value) 0.02；Pressure 1 NMR sample composition：0.3 mM [U-98% 13C; U-98% 15N] STIM1 CC1, 20 mM TRIS, 7 mM [U-13C] SDS, 90 % v/v H2O, 10 % v/v [U-99% 2H] H2O, 90% H2O/10% D2O | 90% H2O/10% D2O | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 6YEL | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2K60 NMR structure of calcium-loaded STIM1 EF-SAM Deposited 2008-07-02 | Different construct Different ligand/ion Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
58–201（144 aa）
Fragment：UNP residues 58-201
|
Not recorded | CA CALCIUM ION × 1 |
SOLUTION NMR
NMR measurement conditions
pH 8；293 K；Ionic strength (raw mmCIF value) 105；Pressure 1
NMR measurement conditions
293 K；Ionic strength (raw mmCIF value) 105；Pressure 1
NMR sample composition
0.5-1.0 mM [U-100% 13C; U-100% 15N] stromal interaction molecule 1,
20 mM TRIS, 100 mM sodium chloride, 5 mM CALCIUM ION, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.5-1.0 mM [U-100% 13C; U-100% 15N] stromal interaction molecule 1,
20 mM [U-99% 2H] TRIS, 100 mM sodium chloride, 5 mM CALCIUM ION, 100% D2O | 100% D2O
|
Resolution not provided |
| 2MAJ Solution Structure of the STIM1 CC1-CC2 homodimer. Deposited 2013-07-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
312–387（76 aa）
Fragment：UNP residues 312-387
Chain C
312–387（76 aa）
Fragment：UNP residues 312-387
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.5；308 K；Ionic strength (raw mmCIF value) 0.02；Pressure ambient
NMR sample composition
0.5 mM [U-99% 15N] protein, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] protein, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] protein, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] protein, 0.5 mM protein, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 100% D2O | 100% D2O
|
Resolution not provided |
| 2MAK Solution structure of the STIM1 CC1-CC2 homodimer in complex with two Orai1 C-terminal domains. Deposited 2013-07-12 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
312–387（76 aa）
Fragment：UNP residues 312-387
Chain C
312–387（76 aa）
Fragment：UNP residues 312-387
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.5；308 K；Ionic strength (raw mmCIF value) 0.02；Pressure ambient
NMR sample composition
0.25 mM [U-100% 15N] protein_1, 3.0 mM protein_2, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.25 mM [U-99% 13C; U-99% 15N] protein_1, 3.0 mM protein_2, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.25 mM [U-99% 13C; U-99% 15N] protein_1, 3.0 mM protein_2, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 100% D2O | 100% D2O
NMR sample composition
2.5 mM protein_1, 0.25 mM [U-99% 15N] protein_2, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
2.5 mM protein_1, 0.25 mM [U-99% 13C; U-99% 15N] protein_2, 20 mM bis-TRIS, 0.175 v/v [U-99% 2H] TFE, 100% D2O | 100% D2O
|
Resolution not provided |
| 3TEQ Crystal structure of SOAR domain Deposited 2011-08-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
344–444（101 aa）
Fragment：SOAR domain (UNP RESIDUES 344-444)
Chain C
344–444（101 aa）
Fragment：SOAR domain (UNP RESIDUES 344-444)
|
Mutation：L374M, V419A, C437T Mutation：L374M, V419A, C437T | PO4 PHOSPHATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1M Bis-Tris pH 6.5, 10% PEG3350, 0.2M Ammonium dibasic phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.249 |
| 3TEQ Crystal structure of SOAR domain Deposited 2011-08-15 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
344–444（101 aa）
Fragment：SOAR domain (UNP RESIDUES 344-444)
|
Mutation：L374M, V419A, C437T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1M Bis-Tris pH 6.5, 10% PEG3350, 0.2M Ammonium dibasic phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.249 |
| 3TEQ Crystal structure of SOAR domain Deposited 2011-08-15 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain D
344–444（101 aa）
Fragment：SOAR domain (UNP RESIDUES 344-444)
|
Mutation：L374M, V419A, C437T | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；277 K；0.1M Bis-Tris pH 6.5, 10% PEG3350, 0.2M Ammonium dibasic phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 1.90 Å R-free 0.249 |
| 4O9B The Structure of CC1-IH in human STIM1. Deposited 2014-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain D
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
|
Mutation：M244L, L321M Mutation：M244L, L321M | CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；24% PEGMME 2000, 0.1M sodium acetate, 0.02M magnesium chloride hexahydrate, 0.02M nickel chloride hexahydrate, 0.02M cadmium chloride hexahydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.289 |
| 4O9B The Structure of CC1-IH in human STIM1. Deposited 2014-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain C
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
|
Mutation：M244L, L321M Mutation：M244L, L321M | CD CADMIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；24% PEGMME 2000, 0.1M sodium acetate, 0.02M magnesium chloride hexahydrate, 0.02M nickel chloride hexahydrate, 0.02M cadmium chloride hexahydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.289 |
| 4O9B The Structure of CC1-IH in human STIM1. Deposited 2014-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain B
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
|
Mutation：M244L, L321M Mutation：M244L, L321M | CD CADMIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；24% PEGMME 2000, 0.1M sodium acetate, 0.02M magnesium chloride hexahydrate, 0.02M nickel chloride hexahydrate, 0.02M cadmium chloride hexahydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.289 |
| 4O9B The Structure of CC1-IH in human STIM1. Deposited 2014-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain D
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
|
Mutation：M244L, L321M Mutation：M244L, L321M | CD CADMIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；24% PEGMME 2000, 0.1M sodium acetate, 0.02M magnesium chloride hexahydrate, 0.02M nickel chloride hexahydrate, 0.02M cadmium chloride hexahydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.289 |
| 4O9B The Structure of CC1-IH in human STIM1. Deposited 2014-01-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain B
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain C
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
Chain D
237–340（104 aa）
Fragment：CC1-IH, UNP RESIDUES 237-340
|
Mutation：M244L, L321M Mutation：M244L, L321M Mutation：M244L, L321M Mutation：M244L, L321M | CD CADMIUM ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；24% PEGMME 2000, 0.1M sodium acetate, 0.02M magnesium chloride hexahydrate, 0.02M nickel chloride hexahydrate, 0.02M cadmium chloride hexahydrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.289 |
5 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | STIM1_HUMAN |
| Isoform | Q13586-2 |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 6–115； UniProt 234–343 |
