6ys3

Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L28

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N3L9_ECOBD
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–78； UniProt 1–78

50S ribosomal protein L29

OrganismNot specified

UniProt A0A140N7F5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N7F5_ECOBD
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 1； PDBConstruct 1–63； UniProt 1–63

50S ribosomal protein L30

OrganismNot specified

UniProt A0A140N7G4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N7G4_ECOBD
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain 2； PDBConstruct 1–59； UniProt 1–59

50S ribosomal protein L32

OrganismNot specified

UniProt A0A140NAZ2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140NAZ2_ECOBD
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain 3； PDBConstruct 1–57； UniProt 1–57

50S ribosomal protein L33

OrganismNot specified

UniProt A0A140N5Z7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5Z7_ECOBD
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain 4； PDBConstruct 1–55； UniProt 1–55

50S ribosomal protein L34

OrganismNot specified

UniProt A0A140NHV0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140NHV0_ECOBD
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain 6； PDBConstruct 1–46； UniProt 1–46

50S ribosomal protein L35

OrganismNot specified

UniProt A0A140N9E3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N9E3_ECOBD
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain 7； PDBConstruct 1–65； UniProt 1–65

50S ribosomal protein L36

OrganismNot specified

UniProt A0A140N5M6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5M6_ECOBD
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain 8； PDBConstruct 1–38； UniProt 1–38

50S ribosomal protein L2

OrganismNot specified

UniProt A0A140N7J1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N7J1_ECOBD
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain c； PDBConstruct 1–273； UniProt 1–273

50S ribosomal protein L3

OrganismNot specified

UniProt A0A140N3G7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N3G7_ECOBD
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain d； PDBConstruct 1–209； UniProt 1–209

50S ribosomal protein L4

OrganismNot specified

UniProt A0A140N5K8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5K8_ECOBD
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain e； PDBConstruct 1–201； UniProt 1–201

50S ribosomal protein L5

OrganismNot specified

UniProt A0A140N5A3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5A3_ECOBD
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain f； PDBConstruct 1–179； UniProt 1–179

50S ribosomal protein L6

OrganismNot specified

UniProt A0A140N2T1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N2T1_ECOBD
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain g； PDBConstruct 1–177； UniProt 1–177

50S ribosomal protein L9

OrganismNot specified

UniProt A0A140NDV1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140NDV1_ECOBD
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain h； PDBConstruct 1–149； UniProt 1–149

50S ribosomal protein L13

OrganismNot specified

UniProt A0A140N598

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N598_ECOBD
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain j； PDBConstruct 1–142； UniProt 1–142

50S ribosomal protein L14

OrganismNot specified

UniProt A0A140N3H4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N3H4_ECOBD
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain k； PDBConstruct 1–123； UniProt 1–123

50S ribosomal protein L15

OrganismNot specified

UniProt A0A140N711

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N711_ECOBD
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain l； PDBConstruct 1–144； UniProt 1–144

50S ribosomal protein L16

OrganismNot specified

UniProt A0A140N6Z2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N6Z2_ECOBD
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain m； PDBConstruct 1–136； UniProt 1–136

50S ribosomal protein L17

OrganismNot specified

UniProt A0A140N4M0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N4M0_ECOBD
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain n； PDBConstruct 1–127； UniProt 1–127

50S ribosomal protein L18

OrganismNot specified

UniProt A0A140N4L0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N4L0_ECOBD
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain o； PDBConstruct 1–117； UniProt 1–117

50S ribosomal protein L19

OrganismNot specified

UniProt A0A140N6T7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N6T7_ECOBD
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain p； PDBConstruct 1–115； UniProt 1–115

50S ribosomal protein L20

OrganismNot specified

UniProt A0A140NBS1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140NBS1_ECOBD
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain q； PDBConstruct 1–118； UniProt 1–118

50S ribosomal protein L21

OrganismNot specified

UniProt A0A140N5D7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5D7_ECOBD
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain r； PDBConstruct 1–103； UniProt 1–103

50S ribosomal protein L22

OrganismNot specified

UniProt A0A140N2S3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N2S3_ECOBD
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain s； PDBConstruct 1–110； UniProt 1–110

50S ribosomal protein L23

OrganismNot specified

UniProt C6EGE8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	C6EGE8_ECOBD
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain t； PDBConstruct 1–100； UniProt 1–100

50S ribosomal protein L24

OrganismNot specified

UniProt A0A140N5L7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N5L7_ECOBD
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain u； PDBConstruct 1–104； UniProt 1–104

50S ribosomal protein L25

OrganismNot specified

UniProt A0A140N846

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L27 × 1 （A0A140N340） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N846_ECOBD
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain w； PDBConstruct 1–94； UniProt 1–94

50S ribosomal protein L27

OrganismNot specified

UniProt A0A140N340

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） Gamma-crystallin B × 1 （P02526）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A140N340_ECOBD
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain y； PDBConstruct 1–85； UniProt 1–85

Gamma-crystallin B

Bos taurus

UniProt P02526

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 29 RNA 3	50S ribosomal protein L28 × 1 （A0A140N3L9） 50S ribosomal protein L29 × 1 （A0A140N7F5） 50S ribosomal protein L30 × 1 （A0A140N7G4） 50S ribosomal protein L32 × 1 （A0A140NAZ2） 50S ribosomal protein L33 × 1 （A0A140N5Z7） 50S ribosomal protein L34 × 1 （A0A140NHV0） 50S ribosomal protein L35 × 1 （A0A140N9E3） 50S ribosomal protein L36 × 1 （A0A140N5M6） 5S rRNA × 1 23S rRNA × 1 50S ribosomal protein L2 × 1 （A0A140N7J1） 50S ribosomal protein L3 × 1 （A0A140N3G7） 50S ribosomal protein L4 × 1 （A0A140N5K8） 50S ribosomal protein L5 × 1 （A0A140N5A3） 50S ribosomal protein L6 × 1 （A0A140N2T1） 50S ribosomal protein L9 × 1 （A0A140NDV1） 50S ribosomal protein L13 × 1 （A0A140N598） 50S ribosomal protein L14 × 1 （A0A140N3H4） 50S ribosomal protein L15 × 1 （A0A140N711） 50S ribosomal protein L16 × 1 （A0A140N6Z2） 50S ribosomal protein L17 × 1 （A0A140N4M0） 50S ribosomal protein L18 × 1 （A0A140N4L0） 50S ribosomal protein L19 × 1 （A0A140N6T7） 50S ribosomal protein L20 × 1 （A0A140NBS1） 50S ribosomal protein L21 × 1 （A0A140N5D7） 50S ribosomal protein L22 × 1 （A0A140N2S3） 50S ribosomal protein L23 × 1 （C6EGE8） 50S ribosomal protein L24 × 1 （A0A140N5L7） glycine-tRNA glyT × 1 50S ribosomal protein L25 × 1 （A0A140N846） 50S ribosomal protein L27 × 1 （A0A140N340）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CRGB_BOVIN
Isoform	—
PDB entities	32
Chains and sequence ranges	Author chain z； PDBConstruct 13–50； UniProt 1–38

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	6ys3
Deposition date deposition_date	2020-04-20
Structure title title	Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide
Keywords keywords	translation 50S ribosome nascent peptide chain, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

6ys3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

6ys3__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

6ys3__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	69.27 Å
Rg (electron density)	69.73 Å
Total Rg	69.78 Å
Atom count	90553
Residues	6217
Excluded volume	1369700 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`6ys3__assembly_1__model_1`	32-meric （32）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (32)

Entity 1 Entity 1 polymer

Description description	50S ribosomal protein L28
Molecular weight formula_weight	9027.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0
Atom count atom_count	90553

MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY

Entity 2 Entity 2 polymer

Description description	50S ribosomal protein L29
Molecular weight formula_weight	7286.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1

MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA

Entity 3 Entity 3 polymer

Description description	50S ribosomal protein L30
Molecular weight formula_weight	6554.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2

MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE

Entity 4 Entity 4 polymer

Description description	50S ribosomal protein L32
Molecular weight formula_weight	6463.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	3

MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK

Entity 5 Entity 5 polymer

Description description	50S ribosomal protein L33
Molecular weight formula_weight	6388.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	4

MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK

Entity 6 Entity 6 polymer

Description description	50S ribosomal protein L34
Molecular weight formula_weight	5397.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	6

MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Entity 7 Entity 7 polymer

Description description	50S ribosomal protein L35
Molecular weight formula_weight	7313.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	7

MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA

Entity 8 Entity 8 polymer

Description description	50S ribosomal protein L36
Molecular weight formula_weight	4377.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	8

MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG

Entity 9 Entity 9 polymer

Description description	5S rRNA
Molecular weight formula_weight	38790.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	a

UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGUGUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAU

Entity 10 Entity 10 polymer

Description description	23S rRNA
Molecular weight formula_weight	942342.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	b

GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCGGUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGAAUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCCAGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGAUACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACAGAGCAAUCUGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUCUUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACUAACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUCUGUAAGCCUGUGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUCGUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGGUAACGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGGCCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGGCCCCUGAUCGGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACUCCUUGAGAGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACC

Entity 11 Entity 11 polymer

Description description	50S ribosomal protein L2
Molecular weight formula_weight	29923.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK

Entity 12 Entity 12 polymer

Description description	50S ribosomal protein L3
Molecular weight formula_weight	22277.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA

Entity 13 Entity 13 polymer

Description description	50S ribosomal protein L4
Molecular weight formula_weight	22121.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA

Entity 14 Entity 14 polymer

Description description	50S ribosomal protein L5
Molecular weight formula_weight	20333.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f

MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTAKSDEEGRALLAAFDFPFRK

Entity 15 Entity 15 polymer

Description description	50S ribosomal protein L6
Molecular weight formula_weight	18932.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g

MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK

Entity 16 Entity 16 polymer

Description description	50S ribosomal protein L9
Molecular weight formula_weight	15789.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h

MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE

Entity 17 Entity 17 polymer

Description description	50S ribosomal protein L13
Molecular weight formula_weight	16050.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j

MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI

Entity 18 Entity 18 polymer

Description description	50S ribosomal protein L14
Molecular weight formula_weight	13565.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k

MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL

Entity 19 Entity 19 polymer

Description description	50S ribosomal protein L15
Molecular weight formula_weight	14994.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l

MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Entity 20 Entity 20 polymer

Description description	50S ribosomal protein L16
Molecular weight formula_weight	15312.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m

MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM

Entity 21 Entity 21 polymer

Description description	50S ribosomal protein L17
Molecular weight formula_weight	14393.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n

MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE

Entity 22 Entity 22 polymer

Description description	50S ribosomal protein L18
Molecular weight formula_weight	12794.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o

MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF

Entity 23 Entity 23 polymer

Description description	50S ribosomal protein L19
Molecular weight formula_weight	13159.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	p

MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN

Entity 24 Entity 24 polymer

Description description	50S ribosomal protein L20
Molecular weight formula_weight	13528.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q

MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA

Entity 25 Entity 25 polymer

Description description	50S ribosomal protein L21
Molecular weight formula_weight	11586.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	r

MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

Entity 26 Entity 26 polymer

Description description	50S ribosomal protein L22
Molecular weight formula_weight	12253.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	s

METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSHITVVVSDR

Entity 27 Entity 27 polymer

Description description	50S ribosomal protein L23
Molecular weight formula_weight	11222.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	t

MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE

Entity 28 Entity 28 polymer

Description description	50S ribosomal protein L24
Molecular weight formula_weight	11339.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	u

MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSETIK

Entity 29 Entity 29 polymer

Description description	glycine-tRNA glyT
Molecular weight formula_weight	23935.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	v

GCGGGCAUCGUAUAAUGGCUAUUACCUCAGCCUUCCAAGCUGAUGAUGCGGGUUCGAUUCCCGCUGCCCGCUCCA

Entity 30 Entity 30 polymer

Description description	50S ribosomal protein L25
Molecular weight formula_weight	10713.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	w

MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA

Entity 31 Entity 31 polymer

Description description	50S ribosomal protein L27
Molecular weight formula_weight	9146.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	y

MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE

Entity 32 Entity 32 polymer

Description description	Gamma-crystallin B
Molecular weight formula_weight	7158.0 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	z

MGHHHHHHHHHHMGKITFYEDRGFQGHCYECSSDCPNLQPYFSRCFSTPVWISQAQGIRAG

Source Source

Source organism organism	Bos taurus
Common name common_name	Cattle
NCBI taxonomy ID ncbi_taxonomy_id	9913
Host organism host_organism	Escherichia coli BL21(DE3)

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6. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id	6ys3g01
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	930 — Outer Surface Protein A; domain 3
Homologous superfamily homologous superfamily	12 — Ribosomal protein L6

Domain ID domain_id	6ys3g02
Class class	3 — Alpha Beta
Architecture architecture	90 — Alpha-Beta Complex
Topology topology	930 — Outer Surface Protein A; domain 3
Homologous superfamily homologous superfamily	12 — Ribosomal protein L6

Domain ID domain_id	6ys3l01
Class class	3 — Alpha Beta
Architecture architecture	100 — Ribosomal Protein L15; Chain: K; domain 2
Topology topology	10 — Ribosomal Protein L15; Chain: K; domain 2
Homologous superfamily homologous superfamily	10 —

▶

Resolution resolution	2.58 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	196254

Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide

1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L28

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L29

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L30

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L32

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L33

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L34

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L35

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L36

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer