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7ej2

human voltage-gated potassium channel KV1.3 H451N mutant

Method: ELECTRON MICROSCOPY Dmax: 163.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Voltage-gated potassium channel subunit beta-2

Homo sapiens

UniProt Q13303

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 1–367 Chain C； UniProt 1–367 Chain E； UniProt 1–367 Chain G； UniProt 1–367 Not recorded Potassium voltage-gated channel subfamily A member 3 × 4 （P22001） NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7EJ2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1ZSX Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2) Deposited 2005-05-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 25–346（322 aa）
Not recorded CL CHLORIDE ION × 16 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；PEG300, sodium/potassium phosphate, sodium chloride, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.172
1ZSX Crystal Structure Of Human Potassium Channel Kv Beta-subunit (KCNAB2) Deposited 2005-05-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 25–346（322 aa）
Not recorded CL CHLORIDE ION × 32 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 8 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.2；293 K；PEG300, sodium/potassium phosphate, sodium chloride, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.90 Å R-free 0.172
7EJ1 human voltage-gated potassium channel KV1.3 Deposited 2021-04-01 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–367（367 aa）
Chain C 1–367（367 aa）
Chain E 1–367（367 aa）
Chain G 1–367（367 aa）
Not recorded NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7WF3 Composite map of human Kv1.3 channel in apo state with beta subunits Deposited 2021-12-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain C 34–361（328 aa）
Chain G 34–361（328 aa）
Chain I 34–361（328 aa）
Chain M 34–361（328 aa）
Not recorded K POTASSIUM ION × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7WF4 Composite map of human Kv1.3 channel in dalazatide-bound state with beta subunits Deposited 2021-12-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain G 34–361（328 aa）
Chain I 34–361（328 aa）
Chain M 34–361（328 aa）
Chain o 34–361（328 aa）
Not recorded NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å

4 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCAB2_HUMAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–367； UniProt 1–367 Author chain C； PDBConstruct 1–367； UniProt 1–367 Author chain E； PDBConstruct 1–367； UniProt 1–367 Author chain G； PDBConstruct 1–367； UniProt 1–367

Potassium voltage-gated channel subfamily A member 3

Homo sapiens

UniProt P22001

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain B； UniProt 1–575 Chain D； UniProt 1–575 Chain F； UniProt 1–575 Chain H； UniProt 1–575 Mutation：H451N Voltage-gated potassium channel subunit beta-2 × 4 （Q13303） NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY cryo-EM buffer：pH 8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.30 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7EJ2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4BGC T1 domain of the renal potassium channel Kv1.3 Deposited 2013-03-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 104–204（101 aa） Fragment：T1 DOMAIN, RESIDUES 104-204
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；WE WANTED TO USE THE PROTEIN IN 150 MM POTASSIUM PHOSPHATE BUFFER PH 6.5 IN 5 MM NMR TUBES FOR CHARACTERIZATION USING NMR SPECTROSCOPY. DURING TESTING, WE OBSERVED THE FORMATION OF CRYSTALS IN THE NMR TUBES. AN INITIAL TEST OF THE CRYSTALS SHOWED AN EXCELLENT DIFFRACTION PATTERN, SO WE DECIDED TO CONTINUE WITH CRYSTALLIZATION OPTIMIZATION IN NMR TUBES, RESULTING IN VERY GOOD CRYSTALS
Resolution 1.20 Å R-free 0.205
7EJ1 human voltage-gated potassium channel KV1.3 Deposited 2021-04-01 Different mutation/modification Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–575（575 aa）
Chain D 1–575（575 aa）
Chain F 1–575（575 aa）
Chain H 1–575（575 aa）
Not recorded NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7SSV Structure of human Kv1.3 with Fab-ShK fusion Deposited 2021-11-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.39 Å
7SSX Structure of human Kv1.3 Deposited 2021-11-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
7SSY Structure of human Kv1.3 (alternate conformation) Deposited 2021-11-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.89 Å
7SSZ Structure of human Kv1.3 with A0194009G09 nanobodies Deposited 2021-11-11 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
7WF3 Composite map of human Kv1.3 channel in apo state with beta subunits Deposited 2021-12-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 208–493（286 aa） Fragment：TM domain
Chain D 208–493（286 aa） Fragment：TM domain
Chain F 208–493（286 aa） Fragment：TM domain
Chain H 208–493（286 aa） Fragment：TM domain
Chain J 99–204（106 aa） Fragment：T1 domain
Chain N 99–204（106 aa） Fragment：T1 domain
Chain O 99–204（106 aa） Fragment：T1 domain
Chain P 99–204（106 aa） Fragment：T1 domain
Not recorded K POTASSIUM ION × 4 NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7WF4 Composite map of human Kv1.3 channel in dalazatide-bound state with beta subunits Deposited 2021-12-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 12 PDB declaration: dodecameric
Chain B 208–490（283 aa） Fragment：TM domain
Chain D 208–490（283 aa） Fragment：TM domain
Chain F 208–490（283 aa） Fragment：TM domain
Chain H 208–490（283 aa） Fragment：TM domain
Chain J 99–204（106 aa） Fragment：T1 domain
Chain N 99–204（106 aa） Fragment：T1 domain
Chain O 99–204（106 aa） Fragment：T1 domain
Chain P 99–204（106 aa） Fragment：T1 domain
Not recorded NAP NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE × 4 K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8DFL Structure of human Kv1.3 with A0194009G09 nanobodies (alternate conformation) Deposited 2022-06-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.25 Å
9CLA Human Kv1.3 mutant-H451V Deposited 2024-07-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.97 Å
9CLV Human Kv1.3 mutant-P424G Deposited 2024-07-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.18 Å
9CON Human Kv1.3-H451V with A0194009G09 nanobodies conformation 1 Deposited 2024-07-17 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.06 Å
9CTY Human Kv1.3-H451V with A0194009G09 nanobodies conformation 2 Deposited 2024-07-25 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.03 Å
9EEF Human Kv1.3 mutant - G427H Deposited 2024-11-19 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Mutation：G427H Mutation：G427H Mutation：G427H Mutation：G427H K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.54 Å
9EI0 Human Kv1.3 mutant - G427H with A0194009G09 nanobodies Deposited 2024-11-25 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–575（575 aa）
Chain B 1–575（575 aa）
Chain C 1–575（575 aa）
Chain D 1–575（575 aa）
Not recorded K POTASSIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.91 Å

15 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name KCNA3_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–575； UniProt 1–575 Author chain D； PDBConstruct 1–575； UniProt 1–575 Author chain F； PDBConstruct 1–575； UniProt 1–575 Author chain H； PDBConstruct 1–575； UniProt 1–575

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7ej2

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7ej2
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7ej2
Deposition date deposition_date2021-04-01
Structure title titlehuman voltage-gated potassium channel KV1.3 H451N mutant
Keywords keywordspotassium channel, complex, C-type inactivation, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier52.35 Å
Radius of gyration Rg (electron density) rg_electron52.64 Å
Forward intensity I(0) i01203930000.00
Molecular weight molecular_weight297290.0 kDa
Excluded volume excluded_volume374890 ų
Envelope volume envelope_volume563830 ų
Hydration-shell volume shell_volume89583 ų
Envelope diameter envelope_diameter163.9 Å
Shell Rg shell_rg55.54 Å
Envelope Rg envelope_rg51.78 Å
Shape Rg shape_rg52.65 Å
Total Rg total_rg52.70 Å
Total atoms total_atoms20968
Residues n_residues2728
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax163.1 Å
Rg (real space) rg_real52.35 Å
Rg uncertainty (real space) rg_real_error1.64 Å
I(0) (real space) i0_real1.2040e+09
I(0) uncertainty (real space) i0_real_error2.5520e+07
Rg (reciprocal space) rg_reciprocal52.34 Å
I(0) (reciprocal space) i0_reciprocal1204000000.0000
Solution quality estimate total_estimate0.8494
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary59.8 Å
Skewness Skewness skewness0.265
Kurtosis Kurtosis kurtosis-0.688
Angular range angular_range— – 0.1500 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha83550000.0000
Real-space data points n_real_points31
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.920; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.279
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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8. Citations (1)

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9. Files and Curves (10)