Transmembrane protease serine 2
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Other combination Monomer Protein × 1 其他Polymer 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 109–492 | Not recorded | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GBS 4-carbamimidamidobenzoic acid × 1 UNX UNKNOWN LIGAND × 3 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 7；291 K；30.0% (w/v) Jeffamine ED-2001 7.0, 0.1 M HEPES pH7.0 | Resolution 1.95 Å R-free 0.225 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 7MEQ | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 7XYD Crystal structure of TMPRSS2 in complex with Nafamostat Deposited 2022-06-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were mutated to DDDDK | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1M acetic acid/sodium acetate, pH 4.0, 20% w/v PEG 4000
|
Resolution 2.58 Å R-free 0.248 |
| 7XYD Crystal structure of TMPRSS2 in complex with Nafamostat Deposited 2022-06-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were mutated to DDDDK | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；0.1M acetic acid/sodium acetate, pH 4.0, 20% w/v PEG 4000
|
Resolution 2.58 Å R-free 0.248 |
| 7Y0E Crystal structure of TMPRSS2 in complex with Camostat Deposited 2022-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were replaced with DDDDK. | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 5.0, 16% w/v PEG 8000
|
Resolution 2.39 Å R-free 0.239 |
| 7Y0E Crystal structure of TMPRSS2 in complex with Camostat Deposited 2022-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were replaced with DDDDK. | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 5.0, 16% w/v PEG 8000
|
Resolution 2.39 Å R-free 0.239 |
| 7Y0F Crystal structure of TMPRSS2 in complex with UK-371804 Deposited 2022-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：Residues 250-255 (SSRQSR) in the database have been replaced with DDDDK. | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 I9V 2-[(1-carbamimidamido-4-chloranyl-isoquinolin-7-yl)sulfonylamino]-2-methyl-propanoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 5.0, 16% w/v PEG 8000
|
Resolution 2.60 Å R-free 0.237 |
| 7Y0F Crystal structure of TMPRSS2 in complex with UK-371804 Deposited 2022-06-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：Residues 250-255 (SSRQSR) in the database have been replaced with DDDDK. | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 I9V 2-[(1-carbamimidamido-4-chloranyl-isoquinolin-7-yl)sulfonylamino]-2-methyl-propanoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 5.0, 16% w/v PEG 8000
|
Resolution 2.60 Å R-free 0.237 |
| 8HD8 Crystal structure of TMPRSS2 in complex with 212-148 Deposited 2022-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were replaced with DDDDK. | CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 4.0, 20% w/v PEG 4000
|
Resolution 2.40 Å R-free 0.218 |
| 8HD8 Crystal structure of TMPRSS2 in complex with 212-148 Deposited 2022-11-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：Residues 250-255 SSRQSR were replaced with DDDDK. | CA CALCIUM ION × 1 GBS 4-carbamimidamidobenzoic acid × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；0.1M acetic acid/sodium acetate, pH 4.0, 20% w/v PEG 4000
|
Resolution 2.40 Å R-free 0.218 |
| 8JHZ Cryo-EM structure of the TcsH-TMPRSS2 complex Deposited 2023-05-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
106–492（387 aa）
|
Not recorded | ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
|
Resolution 3.20 Å |
| 8JI0 Cryo-EM structure of the TcsH-CROP in complex with TMPRSS2 Deposited 2023-05-25 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
106–492（387 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8S0L Crystal structure of the TMPRSS2 zymogen in complex with the nanobody A07 Deposited 2024-02-14 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
107–492（386 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；291 K；20 %w/v PEG 3350, 0.05 M HEPES (pH 7.0), 1 %w/v Tryptone, 0.001 %w/v NaN3
|
Resolution 1.80 Å R-free 0.215 |
| 8S0M Crystal structure of the HKU1 receptor binding domain in complex with TMPRSS2 and the nanobody A01 Deposited 2024-02-14 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
107–492（386 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.35 M NaH2PO4, 0.65 M K2HPO4
|
Resolution 3.55 Å R-free 0.221 |
| 8S0M Crystal structure of the HKU1 receptor binding domain in complex with TMPRSS2 and the nanobody A01 Deposited 2024-02-14 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain E
107–492（386 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.35 M NaH2PO4, 0.65 M K2HPO4
|
Resolution 3.55 Å R-free 0.221 |
| 8S0N Crystal structure of the TMPRSS2 zymogen in complex with the nanobody A07 Deposited 2024-02-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
107–492（386 aa）
|
Mutation：S441A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；10 %w/v PEG 3000, 0.1 M imidazole (pH 8.0), 0.2 M lithium sulfate
|
Resolution 2.30 Å R-free 0.246 |
| 8S0N Crystal structure of the TMPRSS2 zymogen in complex with the nanobody A07 Deposited 2024-02-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
107–492（386 aa）
|
Mutation：S441A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291 K；10 %w/v PEG 3000, 0.1 M imidazole (pH 8.0), 0.2 M lithium sulfate
|
Resolution 2.30 Å R-free 0.246 |
| 8V04 High resolution TMPRSS2 structure following acylation by nafamostat Deposited 2023-11-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
148–255（108 aa）
Chain B
256–492（237 aa）
Fragment：Peptidase S1 domain residues 256-492
|
Mutation：S251D, R252D, Q253D, S254D, R255K | EDO 1,2-ETHANEDIOL × 9 UNX UNKNOWN LIGAND × 1 GBS 4-carbamimidamidobenzoic acid × 1 CIT CITRIC ACID × 1 CA CALCIUM ION × 1 PG4 TETRAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；291 K；3 uL hanging drop (2:1 protein:precipitant) grown over precipitant solution containing 25%PEG4000, 0.2M ammonium sulfate, and 0.1M sodium acetate pH 4.6. Protein (10 mg/mL) was in a buffer containing 25 mM Tris pH 8.0, 75 mM NaCl, and 2 mM CaCl2
|
Resolution 1.58 Å R-free 0.181 |
| 8V1F TMPRSS2 complexed with the noncovalent inhibitor 6-amidino-2-napthol Deposited 2023-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
148–255（108 aa）
Fragment：SRCR domain non-catalytic chain residues 148-254
Chain B
256–492（237 aa）
Fragment：Peptidase S1 domain residues 256-492
|
Mutation：S251D, R252D, Q253D, S254D, R255K | TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 3 UNX UNKNOWN LIGAND × 1 EDO 1,2-ETHANEDIOL × 12 7R8 6-oxidanylnaphthalene-2-carboximidamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；precipitant containing 20% PEG3350, 0.2 M dibasic ammonium citrate. Protein mixed 1uL:1uL protein:precipitant and set as 2 uL hanging drop on glass slides
|
Resolution 2.19 Å R-free 0.223 |
| 8V1F TMPRSS2 complexed with the noncovalent inhibitor 6-amidino-2-napthol Deposited 2023-11-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Other combination Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
148–255（108 aa）
Fragment：SRCR domain non-catalytic chain residues 148-254
Chain D
256–492（237 aa）
Fragment：Peptidase S1 domain residues 256-492
|
Mutation：S251D, R252D, Q253D, S254D, R255K | TAM TRIS(HYDROXYETHYL)AMINOMETHANE × 1 UNX UNKNOWN LIGAND × 2 EDO 1,2-ETHANEDIOL × 8 7R8 6-oxidanylnaphthalene-2-carboximidamide × 1 PEG DI(HYDROXYETHYL)ETHER × 1 CIT CITRIC ACID × 2 PG4 TETRAETHYLENE GLYCOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；precipitant containing 20% PEG3350, 0.2 M dibasic ammonium citrate. Protein mixed 1uL:1uL protein:precipitant and set as 2 uL hanging drop on glass slides
|
Resolution 2.19 Å R-free 0.223 |
| 8VGT Structure of the HKU1 RBD bound to the human TMPRSS2 receptor Deposited 2023-12-27 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8Y1D 2up-TM conformation of HKU1-B S protein after incubation of the receptor Deposited 2024-01-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
109–492（384 aa）
Chain E
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 51 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8Y1E 3up-TM conformation of HKU1-B S protein after incubation of the receptor Deposited 2024-01-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain D
109–492（384 aa）
Chain E
109–492（384 aa）
Chain F
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 51 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8Y7X Structure of HCoV-HKU1A spike in the functionally anchored-3up conformation with 3TMPRSS2 Deposited 2024-02-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain x
109–492（384 aa）
Chain y
109–492（384 aa）
Chain z
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 23 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.09 Å |
| 8Y7Y Local structure of HCoV-HKU1A spike in complex with TMPRSS2 and glycan Deposited 2024-02-05 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric |
Chain T
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 MJJ methyl 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.24 Å |
| 8Y87 Structure of HCoV-HKU1C spike in the functionally anchored-1up conformation with 1TMPRSS2 Deposited 2024-02-06 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain T
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 22 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 8Y88 Structure of HCoV-HKU1C spike in the functionally anchored-2up conformation with 2TMPRSS2 Deposited 2024-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain I
109–492（384 aa）
Chain T
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 18 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 8Y89 Structure of HCoV-HKU1C spike in the functionally anchored-3up conformation with 2TMPRSS2 Deposited 2024-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain G
109–492（384 aa）
Chain T
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 13 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.32 Å |
| 8Y8A Structure of HCoV-HKU1C spike in the functionally anchored-3up conformation with 3TMPRSS2 Deposited 2024-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain G
109–492（384 aa）
Chain H
109–492（384 aa）
Chain T
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.19 Å |
| 8Y8B Local structure of HCoV-HKU1C spike in complex with TMPRSS2 and glycan Deposited 2024-02-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
109–492（384 aa）
Chain T
109–492（384 aa）
|
Not recorded | MJJ methyl 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.01 Å |
| 8YOY Structure of HKU1A RBD with TMPRSS2 Deposited 2024-03-14 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
109–492（384 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.21 Å |
| 8YQQ Structure of HKU1B RBD with TMPRSS2 Deposited 2024-03-19 | Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
109–492（384 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.95 Å |
| 9E83 TMPRSS2 crystal structure following acylation by UCSF_157 Deposited 2024-11-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
148–255（108 aa）
Chain B
256–492（237 aa）
Fragment：Peptidase S1 domain residues 256-492
|
Mutation：S251D/R252D/Q253D/S254D/R255K | CIT CITRIC ACID × 1 EDO 1,2-ETHANEDIOL × 3 VU4 4-(2-aminoethyl)benzoic acid × 1 UNX UNKNOWN LIGAND × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；291 K；3 uL hanging drop (2:1 protein:precipitant) grown over precipitant solution containing 25%PEG4000, 0.2M ammonium sulfate, and 0.1M sodium acetate pH 4.6. Protein (10 mg/mL) was in a buffer containing 25 mM Tris pH 8.0, 75 mM NaCl, and 2 mM CaCl2
|
Resolution 2.07 Å R-free 0.260 |
| 9IZN Crystal structure of HKU1A RBD bound to TMPRSS2 Deposited 2024-08-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
109–492（384 aa）
|
Mutation：R255Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；291 K；2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole (pH 7.0) and 8% w/v Polyethylene glycol 3350
|
Resolution 2.40 Å R-free 0.261 |
| 9JCX Crystal structure of the HCoV-HKU1 RBD and TMPRSS2 Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K Mutation：S441A | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；0.2 M Tris pH 7.0, 0.2 M MgCl2, 10% (w/v) Polyethylene glycol 8,000
|
Resolution 2.75 Å R-free 0.237 |
| 9JD0 Crystal structure of TMPRSS2 in complex with nanobody Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
109–254（146 aa）
Chain C
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GOL GLYCEROL × 1 MLI MALONATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；0.2 M sodium malonate pH 6.0, 10% (w/v) Polyethylene glycol 3,350
|
Resolution 2.00 Å R-free 0.221 |
| 9JD0 Crystal structure of TMPRSS2 in complex with nanobody Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain B
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 MLI MALONATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；0.2 M sodium malonate pH 6.0, 10% (w/v) Polyethylene glycol 3,350
|
Resolution 2.00 Å R-free 0.221 |
| 9JD1 Crystal structure of TMPRSS2 in complex with Fab Deposited 2024-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K | MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 GOL GLYCEROL × 2 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；50 mM MES pH 5.6, 8.6% (w/v) Polyethylene glycol 4,000, 17.1% (v/v) Polyethylene glycol 600
|
Resolution 1.90 Å R-free 0.223 |
| 9K3T Cryo-EM structure of TMPRSS2 in complex with Fab fragments of 752 mAb and 2228 mAb Deposited 2024-10-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
109–492（384 aa）
|
Mutation：250SSRQSR255 replaced with DDDDDK | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.15 Å |
| 9OPQ TMPRSS2 (S441A) bound to the HCoV-NL63 S2'region genetically fused to the HCoV-HKU1 RBD Deposited 2025-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
110–492（383 aa）
|
Mutation：S441A | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9OPR TMPRSS2 S441A in complex with the H1H7 Fab and anti-kappa light chain nanobody Deposited 2025-05-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
110–492（383 aa）
|
Mutation：S441A | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9U8G Crystal structure of TMPRSS2 in complex with nanobody77_10 Deposited 2025-03-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
109–254（146 aa）
Chain B
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；100 mM HEPES/ Sodium hydroxide pH 7.0, 10% w/v PEG 6000
|
Resolution 2.00 Å R-free 0.230 |
| 9U8G Crystal structure of TMPRSS2 in complex with nanobody77_10 Deposited 2025-03-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
109–254（146 aa）
Chain D
256–492（237 aa）
|
Mutation：S250D,S251D,R252D,Q253D, S254K | CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 GOL GLYCEROL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
EVAPORATION；293 K；100 mM HEPES/ Sodium hydroxide pH 7.0, 10% w/v PEG 6000
|
Resolution 2.00 Å R-free 0.230 |
| 9Z3J HCoV-NL63 S2' peptide bound to TMPRSS2 S441A (complexed with the H1H7 Fab and an anti-kappa-nanobody) Deposited 2025-11-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
110–249（140 aa）
Chain B
256–492（237 aa）
|
Mutation：S441A Mutation：S441A | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
33 other PDB entries and 42 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | TMPS2_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–383； UniProt 109–492 |