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7nk4

1918 H1N1 Viral influenza polymerase heterotrimer with Nb8203 core

Method: ELECTRON MICROSCOPY Dmax: 146.1 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Polymerase acidic protein

Influenza A virus (A/Brevig Mission/1/1918(H1N1))

UniProt Q3HM39

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 4 RNA 2 PDB declaration: hexameric（6） Consistent with all polymer counts Chain A； UniProt 1–716 Not recorded RNA-directed RNA polymerase catalytic subunit × 1 （Q3HM40） Polymerase basic protein 2,Polymerase basic protein 2 × 1 （Q3HM41） ;RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') ; × 1 ;RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') ; × 1 Nanobody 8203 × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.32 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7NK4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7NHA 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease and priming loop ordered (Class2a) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.91 Å
7NHC 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease ordered (Class2b) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.87 Å
7NHX 1918 H1N1 Viral influenza polymerase heterotrimer - full transcriptase (Class1) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.23 Å
7NI0 1918 H1N1 Viral influenza polymerase heterotrimer - Replicase (class 3) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.32 Å
7NIK 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8189 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.20 Å
7NIL 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8190 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.01 Å
7NIR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8191 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.70 Å
7NIS 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8192 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.96 Å
7NJ3 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8196 core Deposited 2021-02-15 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.48 Å
7NJ4 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8198 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.84 Å
7NJ5 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8199 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.63 Å
7NJ7 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8200 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.82 Å
7NK1 1918 Influenza virus polymerase heterotirmer in complex with vRNA promoters and Nb8201 Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.22 Å
7NK2 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8202 core Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.84 Å
7NK6 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8204 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.72 Å
7NK8 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8205 core Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.34 Å
7NKA 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8206 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.07 Å
7NKC 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8207 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.46 Å
7NKI 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8209 core Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.67 Å
7NKR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8210 Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
8R60 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide Deposited 2023-11-19 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
8R65 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide with ordered PB2 C-terminal domains Deposited 2023-11-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.23 Å
9RVE Structure of human 1918 influenza A polymerase heterotrimer in complex with 1918 NEP. Deposited 2025-07-08 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
9RYC Structure of human 1918 influenza A polymerase heterotrimer in complex with WSN NEP. Deposited 2025-07-15 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–716（716 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å

24 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PA_I18A0
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–716； UniProt 1–716

RNA-directed RNA polymerase catalytic subunit

Influenza A virus (A/Brevig Mission/1/1918(H1N1))

UniProt Q3HM40

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 4 RNA 2 PDB declaration: hexameric（6） Consistent with all polymer counts Chain B； UniProt 1–757 Not recorded Polymerase acidic protein × 1 （Q3HM39） Polymerase basic protein 2,Polymerase basic protein 2 × 1 （Q3HM41） ;RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') ; × 1 ;RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') ; × 1 Nanobody 8203 × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.32 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7NK4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7NHA 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease and priming loop ordered (Class2a) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.91 Å
7NHC 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease ordered (Class2b) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.87 Å
7NHX 1918 H1N1 Viral influenza polymerase heterotrimer - full transcriptase (Class1) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.23 Å
7NI0 1918 H1N1 Viral influenza polymerase heterotrimer - Replicase (class 3) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.32 Å
7NIK 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8189 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.20 Å
7NIL 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8190 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.01 Å
7NIR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8191 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.70 Å
7NIS 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8192 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.96 Å
7NJ3 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8196 core Deposited 2021-02-15 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.48 Å
7NJ4 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8198 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.84 Å
7NJ5 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8199 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.63 Å
7NJ7 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8200 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.82 Å
7NK1 1918 Influenza virus polymerase heterotirmer in complex with vRNA promoters and Nb8201 Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.22 Å
7NK2 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8202 core Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.84 Å
7NK6 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8204 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.72 Å
7NK8 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8205 core Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.34 Å
7NKA 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8206 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.07 Å
7NKC 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8207 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.46 Å
7NKI 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8209 core Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.67 Å
7NKR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8210 Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
8R60 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide Deposited 2023-11-19 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
8R65 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide with ordered PB2 C-terminal domains Deposited 2023-11-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.23 Å
9RVE Structure of human 1918 influenza A polymerase heterotrimer in complex with 1918 NEP. Deposited 2025-07-08 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
9RYC Structure of human 1918 influenza A polymerase heterotrimer in complex with WSN NEP. Deposited 2025-07-15 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain B 1–757（757 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å

24 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RDRP_I18A0
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–757； UniProt 1–757

Polymerase basic protein 2,Polymerase basic protein 2

Influenza A virus (A/Brevig Mission/1/1918(H1N1))

UniProt Q3HM41

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 4 RNA 2 PDB declaration: hexameric（6） Consistent with all polymer counts Chain C； UniProt 1–759 Not recorded Polymerase acidic protein × 1 （Q3HM39） RNA-directed RNA polymerase catalytic subunit × 1 （Q3HM40） ;RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3') ; × 1 ;RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3') ; × 1 Nanobody 8203 × 1 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 5.32 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 7NK4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
7NHA 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease and priming loop ordered (Class2a) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.91 Å
7NHC 1918 H1N1 Viral influenza polymerase heterotrimer - Endonuclease ordered (Class2b) Deposited 2021-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 2.87 Å
7NHX 1918 H1N1 Viral influenza polymerase heterotrimer - full transcriptase (Class1) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.23 Å
7NI0 1918 H1N1 Viral influenza polymerase heterotrimer - Replicase (class 3) Deposited 2021-02-11 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: pentameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；3.5 second blot and 3 microlitres sample.
Resolution 3.32 Å
7NIK 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8189 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.20 Å
7NIL 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8190 core Deposited 2021-02-12 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.01 Å
7NIR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8191 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.70 Å
7NIS 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8192 core Deposited 2021-02-13 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.96 Å
7NJ3 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8196 core Deposited 2021-02-15 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.48 Å
7NJ4 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8198 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.84 Å
7NJ5 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8199 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.63 Å
7NJ7 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8200 core Deposited 2021-02-16 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.82 Å
7NK1 1918 Influenza virus polymerase heterotirmer in complex with vRNA promoters and Nb8201 Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.22 Å
7NK2 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8202 core Deposited 2021-02-17 Different oligomeric state Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.84 Å
7NK6 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8204 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.72 Å
7NK8 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8205 core Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.34 Å
7NKA 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8206 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.07 Å
7NKC 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8207 Deposited 2021-02-17 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.46 Å
7NKI 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8209 core Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.67 Å
7NKR 1918 H1N1 Viral influenza polymerase heterotrimer with Nb8210 Deposited 2021-02-18 Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.60 Å
8R60 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide Deposited 2023-11-19 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.23 Å
8R65 1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide with ordered PB2 C-terminal domains Deposited 2023-11-20 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 4 PDB declaration: hexameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.23 Å
9RVE Structure of human 1918 influenza A polymerase heterotrimer in complex with 1918 NEP. Deposited 2025-07-08 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.53 Å
9RYC Structure of human 1918 influenza A polymerase heterotrimer in complex with WSN NEP. Deposited 2025-07-15 Different oligomeric state Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 1–759（759 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.93 Å

24 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PB2_I18A0
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–759； UniProt 1–759

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 7nk4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 7nk4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id7nk4
Deposition date deposition_date2021-02-17
Structure title title1918 H1N1 Viral influenza polymerase heterotrimer with Nb8203 core
Keywords keywordsInfluenza, RNA polymerase, H1N1, 1918, VIRAL PROTEIN, nanobody; VIRAL PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier40.57 Å
Radius of gyration Rg (electron density) rg_electron40.55 Å
Forward intensity I(0) i0723007000.00
Molecular weight molecular_weight212540.0 kDa
Excluded volume excluded_volume262930 ų
Envelope volume envelope_volume349520 ų
Hydration-shell volume shell_volume71309 ų
Envelope diameter envelope_diameter159.1 Å
Shell Rg shell_rg46.30 Å
Envelope Rg envelope_rg40.98 Å
Shape Rg shape_rg40.58 Å
Total Rg total_rg40.73 Å
Total atoms total_atoms28741
Residues n_residues1815
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax146.1 Å
Rg (real space) rg_real40.68 Å
Rg uncertainty (real space) rg_real_error1.45 Å
I(0) (real space) i0_real7.2300e+08
I(0) uncertainty (real space) i0_real_error1.2720e+07
Rg (reciprocal space) rg_reciprocal40.58 Å
I(0) (reciprocal space) i0_reciprocal722900000.0000
Solution quality estimate total_estimate0.8270
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary48.7 Å
Skewness Skewness skewness0.554
Kurtosis Kurtosis kurtosis0.153
Angular range angular_range— – 0.1950 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha130100000.0000
Real-space data points n_real_points40
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.630; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.956; Smooth: 0.900
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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8. Citations (1)

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9. Files and Curves (10)