8beo

Crystal structure of E. coli glyoxylate carboligase mutant I393A with MAP

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Glyoxylate carboligase

Escherichia coli K-12

UniProt P0AEP7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein homooligomer Homooligomer Protein 4 MAGNESIUM ION × 12 FLAVIN-ADENINE DINUCLEOTIDE × 4 ;3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-{(1S)-1-HYDROXY-1-[(R)-HYDROXY(METHOXY)PHOSPHORYL]ETHYL}-5-(2-{[(S)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM ; × 4 METHYL HYDROGEN (S)-ACETYLPHOSPHONATE × 4 2,3-DIMETHOXY-5-METHYL-1,4-BENZOQUINONE × 4 DI(HYDROXYETHYL)ETHER × 12 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 6 FORMIC ACID × 34 TRIETHYLENE GLYCOL × 2 SODIUM ION × 10 water × 4 Consistent with protein count
2 Protein homooligomer Homooligomer Protein 4 MAGNESIUM ION × 7 FLAVIN-ADENINE DINUCLEOTIDE × 4 ;3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-{(1S)-1-HYDROXY-1-[(R)-HYDROXY(METHOXY)PHOSPHORYL]ETHYL}-5-(2-{[(S)-HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM ; × 4 METHYL HYDROGEN (S)-ACETYLPHOSPHONATE × 2 2,3-DIMETHOXY-5-METHYL-1,4-BENZOQUINONE × 4 DI(HYDROXYETHYL)ETHER × 2 FORMIC ACID × 18 SODIUM ION × 10 (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL × 1 CHLORIDE ION × 1 water × 4 Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name GCL_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 24–616; UniProt 1–593 Author chain B; PDBConstruct 24–616; UniProt 1–593 Author chain C; PDBConstruct 24–616; UniProt 1–593 Author chain D; PDBConstruct 24–616; UniProt 1–593 Author chain E; PDBConstruct 24–616; UniProt 1–593 Author chain F; PDBConstruct 24–616; UniProt 1–593

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8beo
Deposition date deposition_date2022-10-21
Structure title titleCrystal structure of E. coli glyoxylate carboligase mutant I393A with MAP
Keywords keywordsComplex, LYASE; LYASE
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8beo__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8beo__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8beo__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)39.47 Å
Rg (electron density)38.66 Å
Total Rg39.01 Å
Atom count37547
Residues2364
Excluded volume335400 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8beo__assembly_1__model_1 tetrameric (4) Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 8beo__assembly_2__model_1 tetrameric (4) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (14)

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7. Citations (3)