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8bo2

BAM-EspP complex structure with BamA-S425C/EspP-S1299C mutations in nanodisc

Method: ELECTRON MICROSCOPY Dmax: 142.3 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Outer membrane protein assembly factor BamA

Escherichia coli K-12

UniProt B1XD46

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain A； UniProt 24–810 Mutation：S425C Outer membrane protein assembly factor BamB × 1 （P77774） Outer membrane protein assembly factor BamC × 1 （P0A903） Outer membrane protein assembly factor BamD × 1 （P0AC02） Outer membrane protein assembly factor BamE × 1 （P0A937） Serine protease EspP × 1 （Q7BSW5） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name BAMA_ECODH
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–787； UniProt 24–810

Outer membrane protein assembly factor BamB

Escherichia coli K-12

UniProt P77774

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain B； UniProt 1–392 Not recorded Outer membrane protein assembly factor BamA × 1 （B1XD46） Outer membrane protein assembly factor BamC × 1 （P0A903） Outer membrane protein assembly factor BamD × 1 （P0AC02） Outer membrane protein assembly factor BamE × 1 （P0A937） Serine protease EspP × 1 （Q7BSW5） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8BO2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YH3 The structure of BamB from E. coli Deposited 2011-04-27 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 22–392（371 aa） Fragment：RESIDUES 22-392
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；3.5M NH4CL, 0.1M NA-ACETATE PH 4.6
Resolution 2.60 Å R-free 0.270
2YMS Structure and assembly of a b-propeller with nine blades and a new conserved repetitive sequence motif Deposited 2012-10-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 62–191（130 aa） Fragment：FRAGMENTS OF BAMB FROM E. COLI, RESIDUES 62-191
Chain B 113–186（74 aa） Fragment：FRAGMENTS OF BAMB FROM E. COLI, RESIDUES 113-186
Chain C 248–322（75 aa） Fragment：FRAGMENTS OF BAMB FROM E. COLI, RESIDUES 248-322
Chain D 247–320（74 aa） Fragment：FRAGMENTS OF BAMB FROM E. COLI, RESIDUES 249-320
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.10 Å R-free 0.239
3P1L Crystal structure of Escherichia coli BamB, a lipoprotein component of the beta-barrel assembly machinery complex, native crystals. Deposited 2010-09-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa）
Not recorded NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4；295 K；0.1M Citric acid, 2M NaCl, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.60 Å R-free 0.233
3PRW Crystal structure of the lipoprotein BamB Deposited 2010-11-30 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa） Fragment：UNP Residues 21-392
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.9；292 K；100 mM Tris, pH 6.9, 20% PEG 3350, 200 mM ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K
Resolution 1.80 Å R-free 0.204
3Q7M The crystal structure of BamB from the BAM complex in spacegroup I222 Deposited 2011-01-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa） Fragment：UNP residues 21-392
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.18 M triammonium citrate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.65 Å R-free 0.193
3Q7N The crystal structure of BamB from the BAM complex in spacegroup P212121 Deposited 2011-01-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa） Fragment：UNP residues 21-392
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；0.18 M triammonium citrate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 1.77 Å R-free 0.232
3Q7O The crystal structure of BamB from the BAM complex in spacegroup P213 Deposited 2011-01-05 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa） Fragment：UNP residues 21-392
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；298 K；1.0 M lithium sulfate, 2% PEG8000, pH 7.5, temperature 298K
Resolution 2.09 Å R-free 0.263
4PK1 Structure of BamB fused to a BamA POTRA domain fragment Deposited 2014-05-13 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–392（372 aa） Fragment：UNP P0A940 residues 175-424,UNP P77774 residues 21-392
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；The BamA-BamB chimera protein was crystallized at 16 degC by sitting drop vapor diffusion method. Initial crystallizing conditions were refined in a hanging drop in the following solution: 1.4M Ammonium sulfate, 9% 2-propanol, and 0.1M Sodium acetate pH 5.5. We used 1.5 uL of the 20mg/ml protein mixed with 1.5 uL mother liquor in a reservoir filled with 500 uL precipitant solution
Resolution 3.10 Å R-free 0.281
4XGA Crystal structure of BamB and BamA P3-5 complex from E.coli Deposited 2014-12-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 20–392（373 aa）
Not recorded CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；1% w/v Tryptone, 20% w/v Polyethylene glycol 3350, 0.05M HEPES sodium pH 7.0
Resolution 2.15 Å R-free 0.231
5AYW Structure of a membrane complex Deposited 2015-09-14 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 20–392（373 aa） Fragment：UNP residues 20-392
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；289 K；Tris-HCl, NaCl, PEG 400
Resolution 3.56 Å R-free 0.314
5D0O BamABCDE complex, outer membrane beta barrel assembly machinery entire complex Deposited 2015-08-03 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4
Resolution 2.90 Å R-free 0.304
5LJO E. coli BAM complex (BamABCDE) by cryoEM Deposited 2016-07-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 22–392（371 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Double blot. 3 ul sampel applied and blotted by hand, then additional 3 ul sample applied, blotted, and plunge frozen
Resolution 4.90 Å
6LYQ Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.19 Å R-free 0.293
6LYR Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.28 Å R-free 0.280
6LYS Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.05 Å R-free 0.284
6LYU Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6V05 Cryo-EM structure of a substrate-engaged Bam complex Deposited 2019-11-18 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7BNQ Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7NBX Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-28 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
7NCS Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.10 Å
7ND0 lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
7NRI Structure of the darobactin-bound E. coli BAM complex (BamABCDE) Deposited 2021-03-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；4microL of sample applied
Resolution 3.03 Å
7R1W E. coli BAM complex (BamABCDE) bound to dynobactin A Deposited 2022-02-03 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) n-dodecyl Beta-D-maltopyranoside (DDM)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7TTC BamABCDE bound to substrate EspP Deposited 2022-02-01 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded K33 2,3-dideoxy-6-O-[2-deoxy-4-O-phosphono-2-(tetradecanoylamino)-alpha-L-gulopyranosyl]-1-O-phosphono-beta-D-threo-hexopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ADG Cryo-EM structure of Darobactin 22 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
8ADI Cryo-EM structure of Darobactin 9 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8BVQ E. coli BAM complex (BamABCDE) bound to darobactin B Deposited 2022-12-05 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8BWC E. coli BAM complex (BamABCDE) wild-type Deposited 2022-12-06 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PZ1 Wait Complex: Lateral open BAM bound Compact SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PZ2 Wait Complex: Lateral open BAM bound Extended SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8PZU Wait Complex: BAM bound Darobactin-B and Compact SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PZV Wait Complex: BAM bound Darobactin-B and Extended SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8QN4 Structure of the BAM-EspP complex in an open EspP intermediate state Deposited 2023-09-25 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å
8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Mutation：S425C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
8QPV Handover Complex: BAM bound OmpX and extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8QPW Arrival Complex: Lateral open BAM bound extended SurA plus OmpX Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8SPR The cryo-EM structure of the EcBAM/EspP(beta1-12) complex Deposited 2023-05-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 21–392（372 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8SQA The cryo-EM structure of the EcBAM/EspP(beta8-12) complex Deposited 2023-05-04 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 21–392（372 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8SQB The cryo-EM structure of the EcBAM/EspP(beta7-12) complex Deposited 2023-05-04 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 21–392（372 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9CNW CryoEM structure of the APO-BAM complex in DDM detergent Deposited 2024-07-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.60 Å
9CNX CryoEM structure of BAM in complex with the PTB1 closed-state inhibitor (in DDM detergent) Deposited 2024-07-15 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.55 Å
9CNY CryoEM structure of the APO-BAM complex in SMA nanodisc Deposited 2024-07-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
9CNZ Structure of BAM complexed with PTB2 ligand in detergent Deposited 2024-07-15 Different oligomeric state Different experimental conditions Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CO0 CryoEM structure of BAM in complex with the PTB2 open-state inhibitor (in SMA nanodisc) Deposited 2024-07-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9H84 BAM-hinge (LVPR) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 22–392（371 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
9H85 BAM-hinge (GSGS) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9H89 BAM-hinge (LVPR) suppressor (T434A) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
9HE1 Structure of the Xenorceptide A2-bound E. coli BAM complex (BamABCDE) Deposited 2024-11-13 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7；25 mM NaPi, 100 mM NaCl, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE；3.5uL sample, blot time 3sec, Blotforce 1, Blot total 1,
Resolution 3.00 Å
9HG5 BAM-SurA complex in the swing-in state with full length BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9HG6 BAM-SurA complex in the swing-in state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.73 Å
9HG7 BAM-SurA complex in the swing-out state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
9HG8 BAM-SurA complex in the swing-out state with BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9HG9 BAM-SurA-darobactin complex in the swing-in state Deposited 2024-11-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9HGA BAM-SurA-darobactin complex in the swing-out state Deposited 2024-11-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9MGE beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly Deposited 2024-12-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9MGF beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: heptameric
Chain B 20–392（373 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9MGG beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Deposited 2024-12-10 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9P1U beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly Deposited 2025-06-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–392（392 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å

63 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BAMB_ECOLI
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–392； UniProt 1–392

Outer membrane protein assembly factor BamC

Escherichia coli K-12

UniProt P0A903

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain C； UniProt 1–344 Not recorded Outer membrane protein assembly factor BamA × 1 （B1XD46） Outer membrane protein assembly factor BamB × 1 （P77774） Outer membrane protein assembly factor BamD × 1 （P0AC02） Outer membrane protein assembly factor BamE × 1 （P0A937） Serine protease EspP × 1 （Q7BSW5） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8BO2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2LAE NMR solution structure of the C-terminal domain of the E. coli lipoprotein BamC Deposited 2011-03-11 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 101–344（244 aa） Fragment：sequence database residues 229-344
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.6；303 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 100% D2O | 100% D2O
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 21 mg/ml Pf1 phage, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2LAF NMR solution structure of the N-terminal domain of the E. coli lipoprotein BamC Deposited 2011-03-11 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 101–344（244 aa） Fragment：sequence database residues 101-212
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.6；303 K；Ionic strength (raw mmCIF value) 0.2；Pressure ambient
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 100% D2O | 100% D2O
NMR sample composition 50 mM sodium phosphate, 50 mM sodium chloride, 0.1 mM EDTA, 0.02 % sodium azide, 1 X HALT, 21 mg/ml Pf1 phage, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2YH6 Structure of the N-terminal domain of BamC from E. coli Deposited 2011-04-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 101–212（112 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 101-212
Chain D 101–212（112 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 101-212
Not recorded SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；2 M (NH4)2SO4, 0.1 M CITRIC ACID PH 3.5
Resolution 1.55 Å R-free 0.234
2YH6 Structure of the N-terminal domain of BamC from E. coli Deposited 2011-04-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 101–212（112 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 101-212
Chain B 101–212（112 aa） Fragment：N-TERMINAL DOMAIN, RESIDUES 101-212
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 3.5；2 M (NH4)2SO4, 0.1 M CITRIC ACID PH 3.5
Resolution 1.55 Å R-free 0.234
3SNS Crystal structure of the C-terminal domain of Escherichia coli lipoprotein BamC Deposited 2011-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 224–343（120 aa） Fragment：C-terminal domain (UNP residues 224-343)
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；0.1 M sodium chloride, 0.1 M HEPES, pH 6.5, 1.6 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 1.50 Å R-free 0.183
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 26–344（319 aa） Fragment：UNP residues 26-344
Not recorded K POTASSIUM ION × 1 GOL GLYCEROL × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 26–344（319 aa） Fragment：UNP residues 26-344
Not recorded GOL GLYCEROL × 3 CL CHLORIDE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 26–344（319 aa） Fragment：UNP residues 26-344
Chain D 26–344（319 aa） Fragment：UNP residues 26-344
Not recorded K POTASSIUM ION × 1 GOL GLYCEROL × 4 CL CHLORIDE ION × 1 PO4 PHOSPHATE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 26–344（319 aa） Fragment：UNP residues 26-344
Chain D 26–344（319 aa） Fragment：UNP residues 26-344
Not recorded K POTASSIUM ION × 2 GOL GLYCEROL × 8 CL CHLORIDE ION × 2 PO4 PHOSPHATE ION × 2 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
5AYW Structure of a membrane complex Deposited 2015-09-14 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 33–88（56 aa） Fragment：UNP residues 33-88
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；289 K；Tris-HCl, NaCl, PEG 400
Resolution 3.56 Å R-free 0.314
5D0O BamABCDE complex, outer membrane beta barrel assembly machinery entire complex Deposited 2015-08-03 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4
Resolution 2.90 Å R-free 0.304
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 2–344（343 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain G 2–344（343 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5EKQ The structure of the BamACDE subcomplex from E. coli Deposited 2015-11-04 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain C 25–344（320 aa） Fragment：UNP residues 25-344
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；294 K；100 mM Tris-HCl, pH 8.5, 200 mM MgCl2, 10 mM MnCl2, and 8% PEG 4000
Resolution 3.39 Å R-free 0.281
5LJO E. coli BAM complex (BamABCDE) by cryoEM Deposited 2016-07-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 25–189（165 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；Double blot. 3 ul sampel applied and blotted by hand, then additional 3 ul sample applied, blotted, and plunge frozen
Resolution 4.90 Å
6LYQ Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.19 Å R-free 0.293
6LYR Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.28 Å R-free 0.280
6LYS Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.05 Å R-free 0.284
6LYU Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7BNQ Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7NBX Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-28 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
7NCS Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.10 Å
7ND0 lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
7NRI Structure of the darobactin-bound E. coli BAM complex (BamABCDE) Deposited 2021-03-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；4microL of sample applied
Resolution 3.03 Å
7R1W E. coli BAM complex (BamABCDE) bound to dynobactin A Deposited 2022-02-03 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) n-dodecyl Beta-D-maltopyranoside (DDM)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ADG Cryo-EM structure of Darobactin 22 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
8ADI Cryo-EM structure of Darobactin 9 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8BVQ E. coli BAM complex (BamABCDE) bound to darobactin B Deposited 2022-12-05 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8PZ1 Wait Complex: Lateral open BAM bound Compact SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PZ2 Wait Complex: Lateral open BAM bound Extended SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8PZU Wait Complex: BAM bound Darobactin-B and Compact SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PZV Wait Complex: BAM bound Darobactin-B and Extended SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8QN4 Structure of the BAM-EspP complex in an open EspP intermediate state Deposited 2023-09-25 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å
8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
8QPV Handover Complex: BAM bound OmpX and extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8QPW Arrival Complex: Lateral open BAM bound extended SurA plus OmpX Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
8SPR The cryo-EM structure of the EcBAM/EspP(beta1-12) complex Deposited 2023-05-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 26–344（319 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9H84 BAM-hinge (LVPR) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 25–91（67 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
9H85 BAM-hinge (GSGS) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9H89 BAM-hinge (LVPR) suppressor (T434A) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
9HE1 Structure of the Xenorceptide A2-bound E. coli BAM complex (BamABCDE) Deposited 2024-11-13 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7；25 mM NaPi, 100 mM NaCl, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE；3.5uL sample, blot time 3sec, Blotforce 1, Blot total 1,
Resolution 3.00 Å
9HG5 BAM-SurA complex in the swing-in state with full length BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9HG6 BAM-SurA complex in the swing-in state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.73 Å
9HG7 BAM-SurA complex in the swing-out state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
9HG8 BAM-SurA complex in the swing-out state with BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9HG9 BAM-SurA-darobactin complex in the swing-in state Deposited 2024-11-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9HGA BAM-SurA-darobactin complex in the swing-out state Deposited 2024-11-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9MGE beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9MGF beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: heptameric
Chain C 25–344（320 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9MGG beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Deposited 2024-12-10 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9P1U beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly Deposited 2025-06-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–344（344 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å

51 other PDB entries and 56 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BAMC_ECOLI
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–344； UniProt 1–344

Outer membrane protein assembly factor BamD

Escherichia coli K-12

UniProt P0AC02

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain D； UniProt 1–245 Not recorded Outer membrane protein assembly factor BamA × 1 （B1XD46） Outer membrane protein assembly factor BamB × 1 （P77774） Outer membrane protein assembly factor BamC × 1 （P0A903） Outer membrane protein assembly factor BamE × 1 （P0A937） Serine protease EspP × 1 （Q7BSW5） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8BO2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YHC Structure of BamD from E. coli Deposited 2011-04-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–245（217 aa） Fragment：RESIDUES 29-245
Not recorded URE UREA × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5 .
Resolution 1.80 Å R-free 0.233
3Q5M Crystal structure of Escherichia coli BamD Deposited 2010-12-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 23–245（223 aa）
Not recorded IOD IODIDE ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；0.14M KI, 26%-27% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.60 Å R-free 0.261
3Q5M Crystal structure of Escherichia coli BamD Deposited 2010-12-28 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 23–245（223 aa）
Not recorded IOD IODIDE ION × 14 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；0.14M KI, 26%-27% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.60 Å R-free 0.261
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 21–245（225 aa） Fragment：UNP residues 21-245
Not recorded K POTASSIUM ION × 1 GOL GLYCEROL × 1 PO4 PHOSPHATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 21–245（225 aa） Fragment：UNP residues 21-245
Not recorded GOL GLYCEROL × 3 CL CHLORIDE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 21–245（225 aa） Fragment：UNP residues 21-245
Chain B 21–245（225 aa） Fragment：UNP residues 21-245
Not recorded K POTASSIUM ION × 1 GOL GLYCEROL × 4 CL CHLORIDE ION × 1 PO4 PHOSPHATE ION × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
3TGO Crystal structure of the E. coli BamCD complex Deposited 2011-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 21–245（225 aa） Fragment：UNP residues 21-245
Chain B 21–245（225 aa） Fragment：UNP residues 21-245
Not recorded K POTASSIUM ION × 2 GOL GLYCEROL × 8 CL CHLORIDE ION × 2 PO4 PHOSPHATE ION × 2 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 9.2；295 K；0.2 M K2HPO4 and 20% PEG3350, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 2.90 Å R-free 0.243
5AYW Structure of a membrane complex Deposited 2015-09-14 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 20–245（226 aa） Fragment：UNP residues 20-245
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；289 K；Tris-HCl, NaCl, PEG 400
Resolution 3.56 Å R-free 0.314
5D0O BamABCDE complex, outer membrane beta barrel assembly machinery entire complex Deposited 2015-08-03 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4
Resolution 2.90 Å R-free 0.304
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain D 2–245（244 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain H 2–245（244 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5EKQ The structure of the BamACDE subcomplex from E. coli Deposited 2015-11-04 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain D 20–245（226 aa） Fragment：UNP residues 20-245
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；294 K；100 mM Tris-HCl, pH 8.5, 200 mM MgCl2, 10 mM MnCl2, and 8% PEG 4000
Resolution 3.39 Å R-free 0.281
6LYQ Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.19 Å R-free 0.293
6LYR Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.28 Å R-free 0.280
6LYS Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.05 Å R-free 0.284
6LYU Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7BNQ Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7NBX Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-28 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
7NCS Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.10 Å
7ND0 lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
7NRI Structure of the darobactin-bound E. coli BAM complex (BamABCDE) Deposited 2021-03-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；4microL of sample applied
Resolution 3.03 Å
7R1W E. coli BAM complex (BamABCDE) bound to dynobactin A Deposited 2022-02-03 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) n-dodecyl Beta-D-maltopyranoside (DDM)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ADG Cryo-EM structure of Darobactin 22 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
8ADI Cryo-EM structure of Darobactin 9 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8BVQ E. coli BAM complex (BamABCDE) bound to darobactin B Deposited 2022-12-05 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8PZ1 Wait Complex: Lateral open BAM bound Compact SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PZ2 Wait Complex: Lateral open BAM bound Extended SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8PZU Wait Complex: BAM bound Darobactin-B and Compact SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PZV Wait Complex: BAM bound Darobactin-B and Extended SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8QN4 Structure of the BAM-EspP complex in an open EspP intermediate state Deposited 2023-09-25 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å
8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
8QPV Handover Complex: BAM bound OmpX and extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8QPW Arrival Complex: Lateral open BAM bound extended SurA plus OmpX Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
9H85 BAM-hinge (GSGS) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9H89 BAM-hinge (LVPR) suppressor (T434A) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
9HE1 Structure of the Xenorceptide A2-bound E. coli BAM complex (BamABCDE) Deposited 2024-11-13 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7；25 mM NaPi, 100 mM NaCl, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE；3.5uL sample, blot time 3sec, Blotforce 1, Blot total 1,
Resolution 3.00 Å
9HG5 BAM-SurA complex in the swing-in state with full length BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9HG6 BAM-SurA complex in the swing-in state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.73 Å
9HG7 BAM-SurA complex in the swing-out state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
9HG8 BAM-SurA complex in the swing-out state with BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9HG9 BAM-SurA-darobactin complex in the swing-in state Deposited 2024-11-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9HGA BAM-SurA-darobactin complex in the swing-out state Deposited 2024-11-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9MGE beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9MGF beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: heptameric
Chain D 20–245（226 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9MGG beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Deposited 2024-12-10 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9P1U beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly Deposited 2025-06-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–245（245 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å

46 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BAMD_ECOLI
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D； PDBConstruct 1–245； UniProt 1–245

Outer membrane protein assembly factor BamE

Escherichia coli K-12

UniProt P0A937

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain E； UniProt 1–113 Not recorded Outer membrane protein assembly factor BamA × 1 （B1XD46） Outer membrane protein assembly factor BamB × 1 （P77774） Outer membrane protein assembly factor BamC × 1 （P0A903） Outer membrane protein assembly factor BamD × 1 （P0AC02） Serine protease EspP × 1 （Q7BSW5） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8BO2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2KM7 Solution Structure of BamE, a component of the outer membrane protein assembly machinery in Escherichia coli Deposited 2009-07-24 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–114（94 aa）
Mutation：C20S No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7；303 K；Pressure ambient
NMR sample composition 2.0 mM [U-100% 13C; U-100% 15N] BamE, 50 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KXX NMR Structure of Escherichia coli BamE, a Lipoprotein Component of the beta-Barrel Assembly Machinery Complex Deposited 2010-05-13 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 21–113（93 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.6；288 K；Ionic strength (raw mmCIF value) 0.02；Pressure ambient
NMR sample composition 0.5 mM [U-100% 13C; U-100% 15N] E. coli protein, 15 % D2O, 85 % H2O, 20 mM Na2HPO4/NaH2PO4, 85% H2O/15% D2O | 85% H2O/15% D2O
Resolution not provided
2YH9 Crystal structure of the dimeric BamE from E. coli Deposited 2011-04-27 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 34–113（80 aa） Fragment：RESIDUES 34-113
Chain B 34–113（80 aa） Fragment：RESIDUES 34-113
Chain C 34–113（80 aa） Fragment：RESIDUES 34-113
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5
Resolution 1.80 Å R-free 0.229
5AYW Structure of a membrane complex Deposited 2015-09-14 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 20–113（94 aa） Fragment：UNP residues 20-113
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；289 K；Tris-HCl, NaCl, PEG 400
Resolution 3.56 Å R-free 0.314
5D0O BamABCDE complex, outer membrane beta barrel assembly machinery entire complex Deposited 2015-08-03 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1 M HEPES, pH7.5, 30% PEG600, CYMAL-4
Resolution 2.90 Å R-free 0.304
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5D0Q BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex Deposited 2015-08-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain I 2–113（112 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；297 K；0.1 M Tris, pH8.0, 0.1 M MgCl2, 24% PEG500 MME.
Resolution 3.50 Å R-free 0.340
5EKQ The structure of the BamACDE subcomplex from E. coli Deposited 2015-11-04 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain E 20–113（94 aa） Fragment：UNP residues 20-113
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；294 K；100 mM Tris-HCl, pH 8.5, 200 mM MgCl2, 10 mM MnCl2, and 8% PEG 4000
Resolution 3.39 Å R-free 0.281
6LYQ Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.19 Å R-free 0.293
6LYR Structure of the BAM complex Deposited 2020-02-15 Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.28 Å R-free 0.280
6LYS Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；27-30% PEG 400, 100mM NaCl
Resolution 3.05 Å R-free 0.284
6LYU Structure of the BAM complex Deposited 2020-02-15 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7BNQ Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7NBX Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM Deposited 2021-01-28 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
7NCS Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.10 Å
7ND0 lateral-open conformation of the wild-type BAM complex (BamABCDE) bound to a bactericidal Fab fragment Deposited 2021-01-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
7NRI Structure of the darobactin-bound E. coli BAM complex (BamABCDE) Deposited 2021-03-03 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；4microL of sample applied
Resolution 3.03 Å
7R1W E. coli BAM complex (BamABCDE) bound to dynobactin A Deposited 2022-02-03 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20 mM Tris pH 8.0, 150 mM NaCl, 0.05% (w/v) n-dodecyl Beta-D-maltopyranoside (DDM)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8ADG Cryo-EM structure of Darobactin 22 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.00 Å
8ADI Cryo-EM structure of Darobactin 9 bound BAM complex Deposited 2022-07-08 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8BVQ E. coli BAM complex (BamABCDE) bound to darobactin B Deposited 2022-12-05 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8PZ1 Wait Complex: Lateral open BAM bound Compact SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8PZ2 Wait Complex: Lateral open BAM bound Extended SurA Deposited 2023-07-26 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8PZU Wait Complex: BAM bound Darobactin-B and Compact SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PZV Wait Complex: BAM bound Darobactin-B and Extended SurA Deposited 2023-07-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8QN4 Structure of the BAM-EspP complex in an open EspP intermediate state Deposited 2023-09-25 Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.36 Å
8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
8QPV Handover Complex: BAM bound OmpX and extended SurA Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8QPW Arrival Complex: Lateral open BAM bound extended SurA plus OmpX Deposited 2023-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.30 Å
9H85 BAM-hinge (GSGS) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9H89 BAM-hinge (LVPR) suppressor (T434A) Deposited 2024-10-28 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
9HE1 Structure of the Xenorceptide A2-bound E. coli BAM complex (BamABCDE) Deposited 2024-11-13 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7；25 mM NaPi, 100 mM NaCl, pH 7.0
cryo-EM vitrification conditions Cryogen ETHANE；3.5uL sample, blot time 3sec, Blotforce 1, Blot total 1,
Resolution 3.00 Å
9HG5 BAM-SurA complex in the swing-in state with full length BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9HG6 BAM-SurA complex in the swing-in state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.73 Å
9HG7 BAM-SurA complex in the swing-out state Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.83 Å
9HG8 BAM-SurA complex in the swing-out state with BamC resolved Deposited 2024-11-19 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9HG9 BAM-SurA-darobactin complex in the swing-in state Deposited 2024-11-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.88 Å
9HGA BAM-SurA-darobactin complex in the swing-out state Deposited 2024-11-19 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8；20mM Tris-HCl, 150mM NaCl, 0.05% DDM
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.62 Å
9MGE beta-barrel assembly machine from Escherichia coli in an early state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9MGF beta-barrel assembly machine from Escherichia coli in a middle state of substrate assembly Deposited 2024-12-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 6 PDB declaration: heptameric
Chain E 20–113（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9MGG beta-barrel assembly machine from Escherichia coli in a late state of substrate assembly Deposited 2024-12-10 Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
9P1U beta-barrel assembly machine from Escherichia coli in an late state of LptD assembly Deposited 2025-06-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–113（113 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å

46 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BAME_ECOLI
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E； PDBConstruct 1–113； UniProt 1–113

Serine protease EspP

Escherichia coli K-12

UniProt Q7BSW5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 6 PDB declaration: hexameric（6） Consistent with protein copy count Chain P； UniProt 948–1300 Mutation：S1299C Outer membrane protein assembly factor BamA × 1 （B1XD46） Outer membrane protein assembly factor BamB × 1 （P77774） Outer membrane protein assembly factor BamC × 1 （P0A903） Outer membrane protein assembly factor BamD × 1 （P0AC02） Outer membrane protein assembly factor BamE × 1 （P0A937） ELECTRON MICROSCOPY cryo-EM buffer：pH 7.8 cryo-EM vitrification conditions：Cryogen ETHANE Resolution 3.10 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 8BO2 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2QOM The crystal structure of the E.coli EspP autotransporter Beta-domain. Deposited 2007-07-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1024–1300（277 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；294 K；0.34M Cymal-1, 1% octylglucoside, 33% PEG 1000, 0.2M NaCl, 0.1M Na cacodylate pH 6.4, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.66 Å R-free 0.284
2QOM The crystal structure of the E.coli EspP autotransporter Beta-domain. Deposited 2007-07-20 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1024–1300（277 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.4；294 K；0.34M Cymal-1, 1% octylglucoside, 33% PEG 1000, 0.2M NaCl, 0.1M Na cacodylate pH 6.4, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.66 Å R-free 0.284
3SLJ Pre-cleavage Structure of the Autotransporter EspP - N1023A mutant Deposited 2011-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 999–1300（302 aa） Fragment：Autotransporter protein espP translocator (UNP residues 999-1300)
Mutation：N1023A C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 9 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；294 K；20% w/v PEG8000, 20% v/v glycerol, pH 7.5, HANGING DROP, temperature 294K
Resolution 2.48 Å R-free 0.237
3SLO Pre-cleavage Structure of the Autotransporter EspP - N1023D mutant Deposited 2011-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 999–1300（302 aa） Fragment：Autotransporter protein espP translocator (UNP residues 999-1300)
Mutation：N1023D C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 8 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；294 K；18% w/v PEG8000, 20% v/v glycerol, 25 mM sodium acetate, pH 7.5, HANGING DROP, temperature 294K
Resolution 2.52 Å R-free 0.258
3SLT Pre-cleavage Structure of the Autotransporter EspP - N1023S Mutant Deposited 2011-06-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 999–1300（302 aa） Fragment：Autotransporter protein espP translocator (UNP residues 999-1300)
Mutation：N1023S C8E (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE × 10 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；294 K；20% w/v PEG8000, 20% v/v glycerol, sodium acetate, pH 7.5, HANGING DROP, temperature 294K
Resolution 2.46 Å R-free 0.266
3SZE Crystal structure of the passenger domain of the E. coli autotransporter EspP Deposited 2011-07-18 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 56–1023（968 aa） Fragment：passenger domain, UNP residues 56-1023
Mutation：S263A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；18 22% PEG 4000, 0.1M HEPES, 0.05 0.15M tri-lithium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.50 Å R-free 0.231
7TSZ BamABCDE bound to substrate EspP class 1 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.50 Å
7TT0 BamABCDE bound to substrate EspP class 2 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TT1 BamABCDE bound to substrate EspP class 4 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TT2 BamABCDE bound to substrate EspP class 3 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7TT3 BamABCDE bound to substrate EspP class 5 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TT4 BamABCDE bound to substrate EspP class 6 Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
7TT5 BamABCDE bound to substrate EspP in the open-sheet EspP state Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TT6 BamABCDE bound to substrate EspP in the intermediate-open EspP state Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
7TT7 BamABCDE bound to substrate EspP in the barrelized EspP/continuous open BamA state Deposited 2022-01-31 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain P 948–1298（351 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
7TTC BamABCDE bound to substrate EspP Deposited 2022-02-01 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 1024–1298（275 aa）
Not recorded K33 2,3-dideoxy-6-O-[2-deoxy-4-O-phosphono-2-(tetradecanoylamino)-alpha-L-gulopyranosyl]-1-O-phosphono-beta-D-threo-hexopyranose × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7YE4 BAM-EspP complex structure with BamA-G431C and G781C/EspP-N1293C and A1043C mutations in nanodisc Deposited 2022-07-05 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1300（353 aa）
Mutation：N1293C, A1043C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7YE6 BAM-EspP complex structure with BamA-N427C/EspP-R1297C mutations in nanodisc Deposited 2022-07-05 Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1300（353 aa）
Mutation：R1297C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8；20 mM Tris-HCl, pH 7.8, 150 mM NaCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8BNZ BAM-EspP complex structure with BamA-G431C/EspP-N1293C mutations in nanodisc Deposited 2022-11-14 Different mutation/modification Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1300（353 aa）
Mutation：N1293C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8Q0G Release Complex: BAM bound EspP and Compact SurA Deposited 2023-07-28 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain P 948–1300（353 aa）
Mutation：S1299C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
8QP5 Release Complex: BAM bound EspP (SurA released) Deposited 2023-09-29 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain P 948–1300（353 aa）
Mutation：S1299C No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
8QPU Release Complex: BAM bound EspP and Extended SurA Deposited 2023-10-03 Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain P 948–1300（353 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.20 Å
8SPR The cryo-EM structure of the EcBAM/EspP(beta1-12) complex Deposited 2023-05-03 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain F 949–1300（352 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
8SQA The cryo-EM structure of the EcBAM/EspP(beta8-12) complex Deposited 2023-05-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain F 1195–1300（106 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å

23 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name ESPP_ECO57
Isoform —
PDB entities 6
Chains and sequence ranges Author chain P； PDBConstruct 1–353； UniProt 948–1300

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 8bo2

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 8bo2
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2. Structure Basics 2. Structure Basics

Entry ID entry_id8bo2
Deposition date deposition_date2022-11-14
Structure title titleBAM-EspP complex structure with BamA-S425C/EspP-S1299C mutations in nanodisc
Keywords keywords;BAM, BamABCDE, EspP, Gram-negative bacteria, outer membrane protein, outer membrane barrel, BamA, BamB, BamC, BamD, BamE, MEMBRANE PROTEIN, Outer membrane protein insertion and release, TRANSPORT PROTEIN ;; TRANSPORT PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier44.53 Å
Radius of gyration Rg (electron density) rg_electron44.11 Å
Forward intensity I(0) i0604791000.00
Molecular weight molecular_weight198830.0 kDa
Excluded volume excluded_volume247020 ų
Envelope volume envelope_volume348170 ų
Hydration-shell volume shell_volume66401 ų
Envelope diameter envelope_diameter141.7 Å
Shell Rg shell_rg49.03 Å
Envelope Rg envelope_rg42.51 Å
Shape Rg shape_rg44.09 Å
Total Rg total_rg44.39 Å
Total atoms total_atoms14037
Residues n_residues1803
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax142.3 Å
Rg (real space) rg_real44.33 Å
Rg uncertainty (real space) rg_real_error1.31 Å
I(0) (real space) i0_real6.0480e+08
I(0) uncertainty (real space) i0_real_error1.0820e+07
Rg (reciprocal space) rg_reciprocal44.53 Å
I(0) (reciprocal space) i0_reciprocal604900000.0000
Solution quality estimate total_estimate0.8991
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary61.0 Å
Skewness Skewness skewness0.069
Kurtosis Kurtosis kurtosis-0.602
Angular range angular_range— – 0.1750 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha42910000.0000
Real-space data points n_real_points36
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.920; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.925
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (6)

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7. Fold Classification (SCOP + CATH) 6 domains

CATH v4.4 (6 domains)

Domain ID domain_id8bo2A01
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily310 — membrane protein fhac
Domain ID domain_id8bo2A02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily310 — membrane protein fhac
Domain ID domain_id8bo2A03
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily310 — membrane protein fhac
Domain ID domain_id8bo2A04
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily310 — membrane protein fhac
Domain ID domain_id8bo2A05
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily310 — membrane protein fhac
Domain ID domain_id8bo2A06
Class class2 — Mainly Beta
Architecture architecture40 — Beta Barrel
Topology topology160 — Porin
Homologous superfamily homologous superfamily50 — membrane protein fhac: a member of the omp85/tpsb transporter family
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8. Citations (1)

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9. Files and Curves (10)