T-cell surface glycoprotein CD8 alpha chain
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 4 PDB declaration: tetrameric（4） Consistent with protein copy count | Chain W； UniProt 22–141 | Not recorded | Anti-CD8 alpha VHH × 2 | X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION；pH 7；291 K；15% PEG 3350 and 0.1M succinic acid pH 7.0 | Resolution 1.90 Å R-free 0.245 |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8EW6 | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AKJ COMPLEX OF THE HUMAN MHC CLASS I GLYCOPROTEIN HLA-A2 AND THE T CELL CORECEPTOR CD8 Deposited 1997-05-21 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain D
22–141（120 aa）
Fragment：EXTRACELLULAR IGSF DOMAIN, RESIDUES 1 - 120, ALPHA CHAIN
Chain E
22–141（120 aa）
Fragment：EXTRACELLULAR IGSF DOMAIN, RESIDUES 1 - 120, ALPHA CHAIN
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；PROTEIN WAS CRYSTALLIZED FROM 12% PEG 20000, 100 MM MES, PH 6.5
|
Resolution 2.65 Å R-free 0.276 |
| 1CD8 CRYSTAL STRUCTURE OF A SOLUBLE FORM OF THE HUMAN T CELL CO-RECEPTOR CD8 AT 2.6 ANGSTROMS RESOLUTION Deposited 1992-01-16 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–135（114 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1Q69 Solution structure of T-cell surface glycoprotein CD8 alpha chain and Proto-oncogene tyrosine-protein kinase LCK fragments Deposited 2003-08-12 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
209–227（19 aa）
Fragment：Human CD8 alpha
|
Not recorded | ZN ZINC ION × 1 |
SOLUTION NMR
NMR measurement conditions
pH 5.1；298 K；Pressure ambient
NMR sample composition
0.5 mM CD8a-Lck-Zn2+, U-15N,13C; 20 mM acetate, 20 mM NaCl, 0.15% beta-mercaptoethanol | 95% H2O/5% D2O
NMR sample composition
0.5 mM CD8a-Lck-Zn2+, U-15N; 20 mM acetate, 20 mM NaCl, 0.15% beta-mercaptoethanol | 95% H2O/5% D2O
NMR sample composition
0.5 mM CD8a-Lck-Zn2+; 20 mM acetate, 20 mM NaCl, 0.15% beta-mercaptoethanol | 100% D2O
NMR sample composition
1.0 mM CD8a-Lck-Cd2+; 20 mM acetate, 20 mM NaCl, 0.15% beta-mercaptoethanol | 100% D2O
|
Resolution not provided |
| 2HP4 Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor Deposited 2006-07-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–135（114 aa）
Fragment：HLA Co-receptor, Ig-like V-type
Chain B
22–135（114 aa）
Fragment：HLA Co-receptor, Ig-like V-type
|
Mutation：C33A/S53N Mutation：C33A/S53N | SO4 SULFATE ION × 5 GOL GLYCEROL × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；291 K；25.5% PEG 8000, 0.085M Sodium Cacodylate, 0.17M Ammonium Sulphate, 15% Glycerol, 3% Ethylene Glycol, pH 6.50, VAPOR DIFFUSION, temperature 291K
|
Resolution 2.10 Å R-free 0.233 |
| 2HP4 Computational design and crystal structure of an enhanced affinity mutant human CD8-alpha-alpha co-receptor Deposited 2006-07-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
22–135（114 aa）
Fragment：HLA Co-receptor, Ig-like V-type
Chain B
22–135（114 aa）
Fragment：HLA Co-receptor, Ig-like V-type
|
Mutation：C33A/S53N Mutation：C33A/S53N | SO4 SULFATE ION × 10 GOL GLYCEROL × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；291 K；25.5% PEG 8000, 0.085M Sodium Cacodylate, 0.17M Ammonium Sulphate, 15% Glycerol, 3% Ethylene Glycol, pH 6.50, VAPOR DIFFUSION, temperature 291K
|
Resolution 2.10 Å R-free 0.233 |
| 3QZW Plasticity of human CD8 binding to peptide-HLA-A*2402 Deposited 2011-03-07 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain G
22–135（114 aa）
Fragment：ectodomain (UNP residues 22-135)
Chain H
22–135（114 aa）
Fragment：ectodomain (UNP residues 22-135)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；288 K；0.1M MES monohydrate, pH 6.5, 12% w/v PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.80 Å R-free 0.260 |
| 3QZW Plasticity of human CD8 binding to peptide-HLA-A*2402 Deposited 2011-03-07 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain I
22–135（114 aa）
Fragment：ectodomain (UNP residues 22-135)
Chain J
22–135（114 aa）
Fragment：ectodomain (UNP residues 22-135)
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；288 K；0.1M MES monohydrate, pH 6.5, 12% w/v PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
Resolution 2.80 Å R-free 0.260 |
| 7UMG Crystal structure of human CD8aa-MR1-Ac-6-FP complex Deposited 2022-04-06 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
22–141（120 aa）
Chain D
22–141（120 aa）
|
Not recorded | 30W N-(6-formyl-4-oxo-3,4-dihydropteridin-2-yl)acetamide × 1 PO4 PHOSPHATE ION × 1 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；298 K；Na K Phosphate, PEG 1K and NaCl
|
Resolution 2.40 Å R-free 0.218 |
| 7UVF Crystal structure of ZED8 Fab complex with CD8 alpha Deposited 2022-05-01 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
22–141（120 aa）
Chain B
22–141（120 aa）
|
Not recorded | GOL GLYCEROL × 1 CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6；292 K；0.1M ammonium acetate
0.1M Bis-Tris pH6.0
17% PEG 10K
|
Resolution 2.60 Å R-free 0.259 |
7 other PDB entries and 9 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | CD8A_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain W； PDBConstruct 1–120； UniProt 22–141 |
