SAXSdb — 8g4u

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1. Protein Identity and Related Structures Protein Identity & Related Structures

EryAIII

Saccharopolyspora erythraea

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count
2	Protein homooligomer	Homooligomer	Protein 2	water × 2	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	Q5UNP4_SACER
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 5–881； UniProt 1484–2360 Author chain B； PDBConstruct 5–881； UniProt 1484–2360 Author chain C； PDBConstruct 5–881； UniProt 1484–2360 Author chain D； PDBConstruct 5–881； UniProt 1484–2360

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	8g4u
Deposition date deposition_date	2023-02-10
Structure title title	Final ketosynthase+acyltransferase of the erythromycin modular polyketide synthase
Keywords keywords	ketosynthase, acyltransferase, cerulenin, multimerization, biosynthetic protein; BIOSYNTHETIC PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8g4u__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8g4u__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8g4u__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	54.29 Å
Rg (electron density)	55.33 Å
Total Rg	55.07 Å
Atom count	11646
Residues	1604
Excluded volume	205760 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8g4u__assembly_1__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download
2	1	`8g4u__assembly_2__model_1`	dimeric （2）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Description description	EryAIII
Molecular weight formula_weight	90954.3 kDa
Mutation mutation	A2357Q
Source method src_method	man
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C,D
Atom count atom_count	22987

GSHMADDPIAIVGMACRFPGGVHNPGELWEFIVGGGDAVTEMPTDRGWDLDALFDPDPQRHGTSYSRHGAFLDGAADFDAAFFGISPREALAMDPQQRQVLETTWELFENAGIDPHSLRGSDTGVFLGAAYQGYGQDAVVPEDSEGYLLTGNSSAVVSGRVAYVLGLEGPAVTVDTACSSSLVALHSACGSLRDGDCGLAVAGGVSVMAGPEVFTEFSRQGGLAVDGRCKAFSAEADGFGFAEGVAVVLLQRLSDARRAGRQVLGVVAGSAINQDGASNGLAAPSGVAQQRVIRKAWARAGITGADVAVVEAHGTGTRLGDPVEASALLATYGKSRGSSGPVLLGSVKSNIGHAQAAAGVAGVIKVVLGLNRGLVPPMLCRGERSPLIEWSSGGVELAEAVSPWPPAADGVRRAGVSAFGVSGTNAHVIIAEPPEPEPLPEPGPVGVLAAANSVPVLLSARTETALAAQARLLESAVDDSVPLTALASALATGRAHLPRRAALLAGDHEQLRGQLRAVAEGVAAPGATTGTASAGGVVFVFPGQGAQWEGMARGLLSVPVFAESIAECDAVLSEVAGFSASEVLEQRPDAPSLERVDVVQPVLFSVMVSLARLWGACGVSPSAVIGHSQGEIAAAVVAGVLSLEDGVRVVALRAKALRALAGKGGMVSLAAPGERARALIAPWEDRISVAAVNSPSSVVVSGDPEALAELVARCEDEGVRAKTLPVDYASHSRHVEEIRETILADLDGISARRAAIPLYSTLHGERRDGADMGPRYWYDNLRSQVRFDEAVSAAVADGHATFVEMSPHPVLTAAVQEIAADAVAIGSLHRDTAEEHLIAELARAHVHGVAVDWRNVFPAAPPVALPNYPFEPQRYWLQPEV

Source Source

Source organism organism	Saccharopolyspora erythraea
NCBI taxonomy ID ncbi_taxonomy_id	1836
Host organism host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	8

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6. Fold Classification (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

Domain ID domain_id	8g4uA01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	47 — Peroxisomal Thiolase; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Thiolase/Chalcone synthase

Domain ID domain_id	8g4uA02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	3290 —

Domain ID domain_id	8g4uA03
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	366 — Malonyl-Coenzyme A Acyl Carrier Protein; domain 2
Homologous superfamily homologous superfamily	10 — Malonyl-Coenzyme A Acyl Carrier Protein, domain 2

Domain ID domain_id	8g4uB01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	47 — Peroxisomal Thiolase; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Thiolase/Chalcone synthase

Domain ID domain_id	8g4uB02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	3290 —

Domain ID domain_id	8g4uB03
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	366 — Malonyl-Coenzyme A Acyl Carrier Protein; domain 2
Homologous superfamily homologous superfamily	10 — Malonyl-Coenzyme A Acyl Carrier Protein, domain 2

Domain ID domain_id	8g4uC01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	47 — Peroxisomal Thiolase; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Thiolase/Chalcone synthase

Domain ID domain_id	8g4uD01
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	47 — Peroxisomal Thiolase; Chain A, domain 1
Homologous superfamily homologous superfamily	10 — Thiolase/Chalcone synthase

Domain ID domain_id	8g4uD02
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	3290 —

Domain ID domain_id	8g4uD03
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	366 — Malonyl-Coenzyme A Acyl Carrier Protein; domain 2
Homologous superfamily homologous superfamily	10 — Malonyl-Coenzyme A Acyl Carrier Protein, domain 2

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Unit-cell length a length_a	71.210 Å
Unit-cell length b length_b	167.940 Å
Unit-cell length c length_c	184.230 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	99.43 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 1 21 1
Space-group number int_tables_number	4
Crystal system crystal_system	monoclinic

Resolution resolution	2.900 Å
Low-resolution limit resolution_low	76.230 Å
R factor r_factor	0.2404
R_work r_work	0.2391
R_free r_free	0.3143

Final ketosynthase+acyltransferase of the erythromycin modular polyketide synthase

1. Protein Identity and Related Structures Protein Identity & Related Structures

EryAIII

State in the Current Structure

Other States of the Same Protein in the Database

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8g4u__assembly_1__model_1

8g4u__assembly_1__model_1 | I(q)

8g4u__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

7. Citations (1)

Final ketosynthase+acyltransferase of the erythromycin modular polyketide synthase

1. Protein Identity and Related Structures Protein Identity & Related Structures

EryAIII

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

2. Structure Basics 2. Structure Basics

3. Official assembly/model SAXS Official SAXS Profiles

8g4u__assembly_1__model_1

8g4u__assembly_1__model_1 | I(q)

8g4u__assembly_1__model_1 | P(r) · Pending

4. Crystallography and Experiment 4. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

5. Entities and Polymers Entities & Polymers (2)

Entity 1 Entity 1 polymer

Polymer Polymer

Source Source

Entity 2 Entity 2 water

6. Fold Classification (SCOP + CATH) 10 domains

CATH v4.4 (10 domains)

7. Citations (1)