Beta-2 adrenergic receptor
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain R； UniProt 1–413 | Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging. cryo-EM vitrification conditions：Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging. | Resolution 3.90 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8GGY | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1GQ4 STRUCTURAL DETERMINANTS OF THE NHERF INTERACTION WITH BETA2AR AND PDGFR Deposited 2001-11-19 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
409–413（5 aa）
Fragment：PDZ1 DOMAIN, RESIDUES 11-94
|
Not recorded | CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.24；CITRIC ACID, LITHIUM CHLORIDE, pH 4.24
|
Resolution 1.90 Å R-free 0.186 |
| 2R4R Crystal structure of the human beta2 adrenoceptor Deposited 2007-08-31 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–365（365 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.40 Å R-free 0.270 |
| 2R4S Crystal structure of the human beta2 adrenoceptor Deposited 2007-08-31 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
24–365（342 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 3.40 Å R-free 0.280 |
| 2RH1 High resolution crystal structure of human B2-adrenergic G protein-coupled receptor. Deposited 2007-10-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–365（103 aa）
|
Mutation：N187E, C54T, C97A Mutation：N187E, C54T, C97A | SO4 SULFATE ION × 6 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC MESOPHASE；pH 6.75；293 K；30-35% v/v PEG 400, 0.1-0.2 M Na2SO4, 0.1 M Bis-tris propane pH 6.5-7.0, 5-7% 1,4-Butanediol, 8-10% Cholesterol, 52-50% Monoolein, pH 6.75, LIPIDIC MESOPHASE, temperature 293K
|
Resolution 2.40 Å R-free 0.232 |
| 3D4S Cholesterol bound form of human beta2 adrenergic receptor. Deposited 2008-05-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A | TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 CLR CHOLESTEROL × 2 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
MESOPHASE；pH 7；293 K；28% v/v PEG 400, 300mM K formate, 100mM Bis-tris propane pH 7.0, 2mM Timolol, MESOPHASE, temperature 293K
|
Resolution 2.80 Å R-free 0.273 |
| 3KJ6 Crystal structure of a Methylated beta2 Adrenergic Receptor-Fab complex Deposited 2009-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
2–365（364 aa）
|
Mutation：N187E | SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
bicelles, vapor diffusion；pH 7；298 K；AmSO4, pH 7, bicelles, vapor diffusion, temperature 298K
|
Resolution 3.40 Å R-free 0.280 |
| 3NY8 Crystal structure of the human beta2 adrenergic receptor in complex with the inverse agonist ICI 118,551 Deposited 2010-07-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A
263–348（86 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
|
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A | CLR CHOLESTEROL × 2 JRZ (2S,3S)-1-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]butan-2-ol × 1 OLA OLEIC ACID × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 PGE TRIETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
293 K；100 mM Tris/HCl pH 7.5-8.0, 200-500 mM Na-formate, 5% 1,4 butanediol, 27-32% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
|
Resolution 2.84 Å R-free 0.291 |
| 3NY9 Crystal structure of the human beta2 adrenergic receptor in complex with a novel inverse agonist Deposited 2010-07-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A
263–348（86 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
|
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A | CLR CHOLESTEROL × 2 JSZ ethyl 4-({(2S)-2-hydroxy-3-[(1-methylethyl)amino]propyl}oxy)-3-methyl-1-benzofuran-2-carboxylate × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
293 K；100 mM Tris/HCl pH 7.5-8, 5% 1,4 butanediol, 220 mM Na-formate, 27% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
|
Resolution 2.84 Å R-free 0.278 |
| 3NYA Crystal structure of the human beta2 adrenergic receptor in complex with the neutral antagonist alprenolol Deposited 2010-07-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A
263–348（86 aa）
Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
|
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A | CLR CHOLESTEROL × 2 JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
293 K；100 mM Bis-Tris Propane pH 6.6-6.8, 120 mM Na-tartrate, 3% 1,3 butanediol, 25-30% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
|
Resolution 3.16 Å R-free 0.290 |
| 3P0G Structure of a nanobody-stabilized active state of the beta2 adrenoceptor Deposited 2010-09-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–230（230 aa）
Fragment：UNP P07550 residues 1-230, 263-365, UNP P00720 residues 2-161
Chain A
263–365（103 aa）
Fragment：UNP P07550 residues 1-230, 263-365, UNP P00720 residues 2-161
|
Mutation：N187E Mutation：N187E | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；293 K；36-44% PEG 400, 100 mM Tris pH 8.0, 4% DMSO, 1% 1,2,3-heptanetriol, twin-syringe mixing method, temperature 293K
|
Resolution 3.50 Å R-free 0.308 |
| 3PDS Irreversible Agonist-Beta2 Adrenoceptor Complex Deposited 2010-10-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
25–230（206 aa）
Chain A
264–348（85 aa）
|
Mutation：H93C,N187E,C265A Mutation：H93C,N187E,C265A | ERC 8-hydroxy-5-[(1R)-1-hydroxy-2-({2-[3-methoxy-4-(3-sulfanylpropoxy)phenyl]ethyl}amino)ethyl]quinolin-2(1H)-one × 1 CLR CHOLESTEROL × 1 SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.7；293 K；26 %(v/v) PEG 400, 200 mM Li2SO4, 4 %(v/v) DMSO, 3.5 %(v/v) 1,4-butandediol, 100 mM MES pH 6.7, Lipidic Cubic Phase, temperature 293K
|
Resolution 3.50 Å R-free 0.283 |
| 3SN6 Crystal structure of the beta2 adrenergic receptor-Gs protein complex Deposited 2011-06-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
29–365（337 aa）
|
Mutation：C54T,C97A,M96T,M98T,N187E | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；293 K；350-450 mM potassium nitrate, 100 mM MES, 1 mM TCEP, 10 mM phosphonoformate, 0.01 mM BI167107, 18-22% PEG400. Crystals were grown in a 10:1 (w:w) MAG 7.7:cholesterol lipid mix. , pH 6.5, Lipidic cubic phase, temperature 293K
|
Resolution 3.20 Å R-free 0.277 |
| 4GBR N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor Deposited 2012-07-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–365（337 aa）
Fragment：UNP RESIDUES 29-365
|
Mutation：M96T, M98T, N187E | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；293 K；PEG300 37%
Bis-Tris propane 0.1M
Ammonium phosphate, dibasic, 0.1M, pH 6.5, lipidic cubic phase, temperature 293K
|
Resolution 3.99 Å R-free 0.282 |
| 4LDE Structure of beta2 adrenoceptor bound to BI167107 and an engineered nanobody Deposited 2013-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–235（207 aa）
Fragment：UNP residues 29-348 with a deletion of residues 235-263
Chain A
264–348（85 aa）
Fragment：UNP residues 29-348 with a deletion of residues 235-263
|
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 NA SODIUM ION × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
|
Resolution 2.79 Å R-free 0.256 |
| 4LDL Structure of beta2 adrenoceptor bound to hydroxybenzylisoproterenol and an engineered nanobody Deposited 2013-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–235（207 aa）
Chain A
264–348（85 aa）
|
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A | XQC 4-[(1R)-1-hydroxy-2-{[1-(4-hydroxyphenyl)-2-methylpropan-2-yl]amino}ethyl]benzene-1,2-diol × 1 NA SODIUM ION × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
|
Resolution 3.10 Å R-free 0.254 |
| 4LDO Structure of beta2 adrenoceptor bound to adrenaline and an engineered nanobody Deposited 2013-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–235（207 aa）
Chain A
264–348（85 aa）
|
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A | 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 ALE L-EPINEPHRINE × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
|
Resolution 3.20 Å R-free 0.254 |
| 4QKX Structure of beta2 adrenoceptor bound to a covalent agonist and an engineered nanobody Deposited 2014-06-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–348（320 aa）
|
Mutation：C919T, C962A, H1093C, M1096T, M1098T, N1187E, C1265A | NA SODIUM ION × 2 35V 4-[(1R)-1-hydroxy-2-({2-[3-methoxy-4-(2-sulfanylethoxy)phenyl]ethyl}amino)ethyl]benzene-1,2-diol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.8；293 K；24-27 % PEG400, 100 mM Tris pH 7.8 to pH 8.4, 5-10 mM Sodium formate, lipidic cubic phase, temperature 293K
|
Resolution 3.30 Å R-free 0.280 |
| 5D5A In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K Deposited 2015-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–365（103 aa）
|
Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol
|
Resolution 2.48 Å R-free 0.262 |
| 5D5B In meso X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K Deposited 2015-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–365（103 aa）
|
Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 1 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol
|
Resolution 3.80 Å R-free 0.274 |
| 5D6L beta2AR-T4L - CIM Deposited 2015-08-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–223（223 aa）
Chain A
264–365（102 aa）
|
Mutation：N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A Mutation：N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A | SO4 SULFATE ION × 6 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；30 - 35 % Peg 400, 5 -10 % butanediol, 0.1 - 0.35 mM Na sulphate, 0.1 M Bis-Tris pH 7.0.
|
Resolution 3.20 Å R-free 0.260 |
| 5JQH Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60 Deposited 2016-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
30–348（319 aa）
Fragment：UNP RESIDUES 2-161, UNP RESIDEUS 30-348
|
Mutation：C919T, C962A, M1096T, M1098T, N1187E, C1265A,C919T, C962A, M1096T, M1098T, N1187E, C1265A | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 CLR CHOLESTEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.5；293 K；100 mM HEPES pH 7.5, 20 mM EDTA, and 19-23% PEG300
|
Resolution 3.20 Å R-free 0.290 |
| 5JQH Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60 Deposited 2016-05-05 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
30–348（319 aa）
Fragment：UNP RESIDUES 2-161, UNP RESIDEUS 30-348
|
Mutation：C919T, C962A, M1096T, M1098T, N1187E, C1265A,C919T, C962A, M1096T, M1098T, N1187E, C1265A | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 CLR CHOLESTEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.5；293 K；100 mM HEPES pH 7.5, 20 mM EDTA, and 19-23% PEG300
|
Resolution 3.20 Å R-free 0.290 |
| 5X7D Structure of beta2 adrenoceptor bound to carazolol and an intracellular allosteric antagonist Deposited 2017-02-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Fragment：UNP P07550 residues 1-230, UNP D9IEF7 residues 2-162, UNP P07550 residues 264-365
Chain A
264–365（102 aa）
Fragment：UNP P07550 residues 1-230, UNP D9IEF7 residues 2-162, UNP P07550 residues 264-365
|
Mutation：N187E, C1054T, C1097A Mutation：N187E, C1054T, C1097A | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 CLR CHOLESTEROL × 2 8VS 4-carbamoyl-N-[(2R)-2-cyclohexyl-2-phenylacetyl]-L-phenylalanyl-3-bromo-N-methyl-L-phenylalaninamide × 1 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 1 ACM ACETAMIDE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.5；293 K；0.1 M Tris-HCl, pH 7.5, 30% -37.5% PEG400, 400 mM NH4F, 6% 1,4-butanediol, 1 mM Cmpd-15PA and 1% DMSO.
|
Resolution 2.70 Å R-free 0.268 |
| 6E67 Structure of beta2 adrenergic receptor fused to a Gs peptide Deposited 2018-07-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–232（232 aa）
Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Chain A
268–365（98 aa）
Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
|
Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7；293 K；100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol
|
Resolution 3.70 Å R-free 0.307 |
| 6E67 Structure of beta2 adrenergic receptor fused to a Gs peptide Deposited 2018-07-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–232（232 aa）
Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Chain B
268–365（98 aa）
Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
|
Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7；293 K；100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol
|
Resolution 3.70 Å R-free 0.307 |
| 6MXT Crystal structure of human beta2 adrenergic receptor bound to salmeterol and Nb71 Deposited 2018-10-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–234（206 aa）
Fragment：chimera of T4 lysozyme fused to human beta2 adrenergic receptor (UNP residues 29-234,263-365)
Chain A
263–365（103 aa）
Fragment：chimera of T4 lysozyme fused to human beta2 adrenergic receptor (UNP residues 29-234,263-365)
|
Mutation：M96T, M98T, N187E Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：M96T, M98T, N187E Non-standard monomer：Yes (specific site not provided by mmCIF) | K5Y salmeterol × 1 MG MAGNESIUM ION × 1 NI NICKEL (II) ION × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 HTO HEPTANE-1,2,3-TRIOL × 1 OLA OLEIC ACID × 1 P33 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 7.5；293.15 K；31-34% PEG400, 100 mM HEPES, pH 7.5, 1% 1,2,3-heptanetriol
|
Resolution 2.96 Å R-free 0.245 |
| 6N48 Structure of beta2 adrenergic receptor bound to BI167107, Nanobody 6B9, and a positive allosteric modulator Deposited 2018-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
29–234（206 aa）
Fragment：chimera of lysozyme and B2AR (UNP residues 29-234,263-348)
Chain A
263–348（86 aa）
Fragment：chimera of lysozyme and B2AR (UNP residues 29-234,263-348)
|
Mutation：M1096T, M1098T, N1187E Mutation：M1096T, M1098T, N1187E | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 KBY N-[(3R)-4-(4-tert-butylphenyl)-3-({2-[(4-methoxyphenyl)sulfanyl]-5-[methyl(propan-2-yl)sulfamoyl]benzene-1-carbonyl}amino)butanoyl]glycine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 8；293 K；100 mM Tris-HCl, pH 8.0, 150-200 mM lithium acetate, 43-45% PEG400
|
Resolution 3.20 Å R-free 0.274 |
| 6NI3 B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex Deposited 2018-12-26 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
29–341（313 aa）
|
Not recorded | P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 6OBA The beta2 adrenergic receptor bound to a negative allosteric modulator Deposited 2019-03-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Fragment：beta-2 (UNP residues 1-230), lysozyme, beta-2 (UNP residues 264-365)
Chain A
264–365（102 aa）
Fragment：beta-2 (UNP residues 1-230), lysozyme, beta-2 (UNP residues 264-365)
|
Mutation：N187E, C1054T, C1097A Mutation：N187E, C1054T, C1097A | CLR CHOLESTEROL × 2 JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 M3J 6-bromo-N~2~-phenylquinazoline-2,4-diamine × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；pH 8；293 K；0.1 M Tris-HCl, pH 8.0, 30-40% PEG400, 300-400 mM sodium formate, 6% 1,4-butanediol, 1 mM alprenolol, 1 mM AS408, 1% DMSO
|
Resolution 3.10 Å R-free 0.277 |
| 6PRZ XFEL beta2 AR structure by ligand exchange from Alprenolol to Alprenolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 OLA OLEIC ACID × 2 SO4 SULFATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% (v/v) PEG 400, 2 mM Alprenolol
|
Resolution 2.80 Å R-free 0.228 |
| 6PS0 XFEL beta2 AR structure by ligand exchange from Alprenolol to Carazolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 OLA OLEIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Carazolol
|
Resolution 3.40 Å R-free 0.260 |
| 6PS1 XFEL beta2 AR structure by ligand exchange from Alprenolol to Timolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 OLA OLEIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Timolol
|
Resolution 3.20 Å R-free 0.264 |
| 6PS2 XFEL beta2 AR structure by ligand exchange from Timolol to Alprenolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 CLR CHOLESTEROL × 1 OLB (2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 SO4 SULFATE ION × 4 OLA OLEIC ACID × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Alprenolol
|
Resolution 2.40 Å R-free 0.224 |
| 6PS3 XFEL beta2 AR structure by ligand exchange from Timolol to Carvedilol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | CVD (2S)-1-(8H-CARBAZOL-4-YLOXY)-3-[2-(2-METHOXYPHENOXY)ETHYLAMINO]PROPAN-2-OL × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 6 OLA OLEIC ACID × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Carvedilol
|
Resolution 2.50 Å R-free 0.242 |
| 6PS4 XFEL beta2 AR structure by ligand exchange from Timolol to ICI-118551. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | JRZ (2S,3S)-1-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]butan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLA OLEIC ACID × 4 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 10 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand ICI-118551
|
Resolution 2.60 Å R-free 0.243 |
| 6PS5 XFEL beta2 AR structure by ligand exchange from Timolol to Propranolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | SNP 1-(ISOPROPYLAMINO)-3-(1-NAPHTHYLOXY)-2-PROPANOL × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLA OLEIC ACID × 2 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Propranolol
|
Resolution 2.90 Å R-free 0.258 |
| 6PS6 XFEL beta2 AR structure by ligand exchange from Timolol to Timolol. Deposited 2019-07-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–230（230 aa）
Chain A
263–348（86 aa）
|
Not recorded | TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 OLA OLEIC ACID × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Timolol
|
Resolution 2.70 Å R-free 0.232 |
| 7DHI Cryo-EM structure of the partial agonist salbutamol-bound beta2 adrenergic receptor-Gs protein complex. Deposited 2020-11-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
1–348（348 aa）
|
Mutation：G16R,E27Q,C77V,M96T,M98T,E122W,C125V,N187E,C265A,C285A,C327S | 68H SALBUTAMOL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 7DHR Cryo-EM structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-Gs protein complex. Deposited 2020-11-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
1–348（348 aa）
|
Mutation：G16R,E27Q,C77V,M96T,M98T,E122W,C125V,N187E,C265A,C285A,C327S | 5FW ISOPRENALINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7XK9 Structure of human beta2 adrenergic receptor bound to constrained isoproterenol Deposited 2022-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–235（207 aa）
Chain A
264–348（85 aa）
|
Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A | GJ6 (5R,6R)-6-(propan-2-ylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；100mM Tris-HCl, pH 8.0, 150-200mM lithium acetate, 43-45% PEG 400
|
Resolution 3.40 Å R-free 0.261 |
| 7XKA Structure of human beta2 adrenergic receptor bound to constrained epinephrine Deposited 2022-04-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–235（207 aa）
Chain A
264–348（85 aa）
|
Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；100mM Tris-HCl, pH 8.0, 150-200mM lithium acetate, 43-45% PEG400
|
Resolution 3.10 Å R-free 0.272 |
| 8GDZ CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#1 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GE1 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#2 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8GE2 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#3 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8GE3 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#4 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8GE4 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#5 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8GE5 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#6 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8GE6 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#7 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8GE7 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#8 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8GE8 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#9 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8GE9 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#10 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GEA CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#11 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GEB CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#12 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 8GEC CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#13 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GED CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#14 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GEE CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#15 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 8GEF CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#16 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8GEG CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#17 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 8GEH CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#18 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 8GEI CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#19 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å |
| 8GEJ CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#20 of 20) Deposited 2023-03-06 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8GFV CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.10 Å |
| 8GFW CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GFX CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GFY CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GFZ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GG0 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 2.90 Å |
| 8GG1 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GG2 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.00 Å |
| 8GG3 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.10 Å |
| 8GG4 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GG5 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GG6 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8GG7 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8GG8 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8GG9 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8GGA CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGB CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGC CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8GGE CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGF CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20) Deposited 2023-03-08 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8GGI Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGJ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGK Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8GGL Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GGM Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GGN Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8GGO Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GGP Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.20 Å |
| 8GGQ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8GGR Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8GGS Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGT Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGU Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8GGV Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8GGW Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8GGX Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8GGZ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20) Deposited 2023-03-08 | Parsed fields agree | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.90 Å |
| 8GH0 Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 4.00 Å |
| 8GH1 Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20) Deposited 2023-03-08 | Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain R
1–413（413 aa）
|
Not recorded | G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 4.10 Å |
| 8JJ8 Cryo-EM structure of the beta2AR-mBRIL/1b3 Fab/Glue complex with a partial agonist Deposited 2023-05-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
29–230（202 aa）
Chain F
263–340（78 aa）
|
Not recorded | H98 ~{N}-[5-[(1~{R})-2-[[(2~{R})-1-(4-methoxyphenyl)propan-2-yl]amino]-1-oxidanyl-ethyl]-2-oxidanyl-phenyl]methanamide × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.20 Å |
| 8JJL cryo-EM structure of the beta2-AR-mBRIL/1b3 Fab/Glue complex with a full agonist Deposited 2023-05-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–230（202 aa）
Chain A
263–340（78 aa）
|
Not recorded | DZQ Olodaterol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.20 Å |
| 8JJO Cryo-EM structure of the beta2AR-mBRIL/1b3 Fab/Glue complex with an antagonist Deposited 2023-05-31 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
29–230（202 aa）
Chain F
263–340（78 aa）
|
Not recorded | JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.40 Å |
| 8UHB Cryo-EM Structure of the Ro5256390-bound hTA1-Gs heterotrimer signaling complex Deposited 2023-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–30（30 aa）
|
Mutation：M(-129)W, R(-14)G, Q(-3)E, F112W | WV8 (2R,4S)-4-[(2S)-2-phenylbutyl]-1,3-oxazolidin-2-amine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 8UNL CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class A) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8UNM CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class B) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8UNN CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class C) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Combined datasets of complex plunge frozen at 5 sec, 10 sec, or 17 sec after GTP addition to the sample.
|
Resolution 3.40 Å |
| 8UNO CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class D) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8UNP CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class E) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8UNQ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class F) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8UNR CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class G) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8UNS CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class H) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8UNT CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class I) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8UNU CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class J) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8UNV CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class K) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.30 Å |
| 8UNW CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class L) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.40 Å |
| 8UNX CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class M) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8UNY CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class N) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8UNZ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class O) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.80 Å |
| 8UO0 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class P) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8UO1 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class Q) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8UO2 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class R) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.60 Å |
| 8UO3 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class S) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 3.50 Å |
| 8UO4 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class T) Deposited 2023-10-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–413（413 aa）
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions
Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
|
Resolution 4.00 Å |
| 8W1V The beta2 adrenergic receptor bound to a bitopic ligand Deposited 2024-02-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain A
1–365（365 aa）
Chain B
1–365（365 aa）
|
Mutation：N187E,C1054T,C1097A Mutation：N187E,C1054T,C1097A | A1AE2 (2S)-1-[(3-{1-[4-(4-{(2S)-2-hydroxy-3-[(propan-2-yl)amino]propoxy}phenyl)butyl]-1H-1,2,3-triazol-4-yl}propyl)amino]-3-(2-propylphenoxy)propan-2-ol × 2 AV0 Lauryl Maltose Neopentyl Glycol × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；100 mM Tris buffer (pH 8.0), 100 to 175 mM lithium sulfate, 38% to 42% PEG400, and 10 mM EDTA
|
Resolution 3.00 Å R-free 0.292 |
| 8ZBE cryo-EM structure of the octreotide-bound SSTR5-Gi complex Deposited 2024-04-26 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain A
1–24（24 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.24 Å |
| 8ZCJ Cryo-EM structure of the pasireotide-bound SSTR5-Gi complex Deposited 2024-04-29 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain G
1–24（24 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.09 Å |
| 8ZWF cryoEM structure of JR14a bound C3aR-BRIL-BAG2 complex Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
1–30（30 aa）
|
Mutation：E27Q,M29W,H124I | A1D9A (2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-methyl-thiophen-2-yl]carbonylamino]pentanoic acid × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8ZWG cryoEM structure of JR14a bound C3aR-Gi complex Deposited 2024-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
1–30（30 aa）
|
Mutation：E27Q | A1D9A (2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-methyl-thiophen-2-yl]carbonylamino]pentanoic acid × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9BUY Structure of the LM189-Bound beta2AR-Gi Complex Deposited 2024-05-17 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
1–413（413 aa）
|
Not recorded | CLR CHOLESTEROL × 3 A1ASM 4-[(1R)-1-hydroxy-2-{[6-(4-phenylbutoxy)hexyl]amino}ethyl]benzene-1,2-diol × 1 KBY N-[(3R)-4-(4-tert-butylphenyl)-3-({2-[(4-methoxyphenyl)sulfanyl]-5-[methyl(propan-2-yl)sulfamoyl]benzene-1-carbonyl}amino)butanoyl]glycine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9CHU Cryo-EM structure of calcineurin fused beta2 adrenergic receptor in norepinephrine bound inactive state Deposited 2024-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
29–230（202 aa）
Chain A
263–336（74 aa）
|
Not recorded | E5E Noradrenaline × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.49 Å |
| 9CHV cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in apo state Deposited 2024-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
29–230（202 aa）
Chain A
263–354（92 aa）
|
Not recorded | FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.95 Å |
| 9CHX cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in carazolol bound inactive state Deposited 2024-07-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric |
Chain A
29–230（202 aa）
Chain A
263–354（92 aa）
|
Not recorded | CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9I52 Dopamine 1 receptor:GaS complex bound to 19B Deposited 2025-01-27 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–30（30 aa）
|
Not recorded | A1IZU 6-(4-imidazo[1,2-a]pyridin-8-yl-2-methyl-phenyl)-1,5-dimethyl-pyrimidine-2,4-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9I54 Dopamine 1 receptor:GaS complex bound to 24 Deposited 2025-01-27 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain R
1–30（30 aa）
|
Not recorded | A1IZV 6-(3-fluoranyl-5-furo[3,2-c]pyridin-4-yloxy-pyridin-2-yl)-1,5-dimethyl-pyrimidine-2,4-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.72 Å |
| 9L8L Structure of GPCR megacomplex bound to agonists Deposited 2024-12-27 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
1–413（413 aa）
|
Mutation：K237R,G276S,I277V | DR7 (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER × 1 P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.22 Å |
| 9LW5 Cryo-EM structure of dopamine bound mut-beta2-Adrenergic Receptor beta2R-M16-miniGs-Gbeta1gamma2-Nb35-scFv16 complex Deposited 2025-02-13 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric |
Chain R
1–348（348 aa）
|
Not recorded | LDP L-DOPAMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.87 Å |
| 9RKF Dark structure of beta-2 Adrenergic receptor with photoazolol in Dark state recorded at SwissFEL Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
28–230（203 aa）
Chain A
264–342（79 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 5 CLR CHOLESTEROL × 3 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 5 1PE PENTAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 PLM PALMITIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；289 K；100mM tri-sodium citrate pH= 6.2, 245mM Li2SO4, 32% PEG 350 MME, 10uM photoazolol-1
|
Resolution 2.45 Å R-free 0.219 |
| 9RKG Mixed model refinement of beta-2 Adrenergic receptor with photoazolol in dark state and Light state, 10 seconds after light activation, recorded at SwissFEL Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–230（202 aa）
Chain A
263–342（80 aa）
|
Not recorded | SO4 SULFATE ION × 6 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 5 1PE PENTAETHYLENE GLYCOL × 3 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
|
Resolution 2.45 Å R-free 0.217 |
| 9RKH Dark structure of beta-2 Adrenergic receptor with photoazolol in Dark state recorded at LCLS Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–230（202 aa）
Chain A
264–342（79 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 5 CLR CHOLESTEROL × 2 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
|
Resolution 2.50 Å R-free 0.218 |
| 9RKI Mixed model refinement of beta-2 Adrenergic receptor with photoazolol in dark state and Light state, 17 nanoseconds after light activation, recorded at LCLS Deposited 2025-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
29–230（202 aa）
Chain A
263–342（80 aa）
|
Not recorded | SO4 SULFATE ION × 7 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 1PE PENTAETHYLENE GLYCOL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
|
Resolution 2.60 Å R-free 0.234 |
| 9U4Y cryo-EM structure of Xenopus laevis GnRHR bound with mammal GnRH Deposited 2025-03-20 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain R
1–30（30 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.67 Å |
| 9U9V beta2-adrenergic receptor in complex with methamphetamine Deposited 2025-03-29 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain R
1–348（348 aa）
|
Not recorded | B40 (2S)-N-methyl-1-phenylpropan-2-amine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.85 Å |
| 9UAM CysLTR2-LTC4-miniGq complex Deposited 2025-04-01 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain A
2–30（29 aa）
|
Not recorded | Y01 CHOLESTEROL HEMISUCCINATE × 1 LTX (5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-( 2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic acid × 1 CLR CHOLESTEROL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9W3F Cryo-EM structure of the human beta2-adrenergic receptor in complex with a novel antagonist Deposited 2025-07-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
1–230（230 aa）
Chain A
264–365（102 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions | Resolution 3.04 Å |
143 other PDB entries and 145 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | ADRB2_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain R； PDBConstruct 25–452； UniProt 1–413 |
