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1gq4

STRUCTURAL DETERMINANTS OF THE NHERF INTERACTION WITH BETA2AR AND PDGFR

Method: X-RAY DIFFRACTION Dmax: 42.7 Å Quality: REASONABLE
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1. Protein Identity and Related Structures Protein Identity & Related Structures

EZRIN-RADIXIN-MOESIN BINDING PHOSPHOPROTEIN-50

HOMO SAPIENS

UniProt O14745

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 11–94 Fragment：PDZ1 DOMAIN, RESIDUES 11-94 CL CHLORIDE ION × 4 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.24；CITRIC ACID, LITHIUM CHLORIDE, pH 4.24 Resolution 1.90 Å R-free 0.186

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1GQ4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1G9O FIRST PDZ DOMAIN OF THE HUMAN NA+/H+ EXCHANGER REGULATORY FACTOR Deposited 2000-11-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–99（89 aa） Fragment：PDZ1 DOMAIN
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5.9；291 K；potassium sodium tartrate, sodium citrate, ammonium sulfate, manganese chloride, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.50 Å R-free 0.201
1GQ5 Structural Determinants of the NHERF Interaction with beta2-AR and PDGFR Deposited 2001-11-20 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：PDZ1 DOMAIN, RESIDUES 11-94
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 4.6；SODIUM ACETATE, SODIUM CHLORIDE, pH 4.60
Resolution 2.20 Å R-free 0.263
1I92 STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION Deposited 2001-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：PDZ1 DOMAIN (RESIDUES 11-94)
Not recorded CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 4.6；291 K；Sodium Acetate, Sodium Chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K
Resolution 1.70 Å R-free 0.217
1SGH Moesin FERM domain bound to EBP50 C-terminal peptide Deposited 2004-02-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 321–358（38 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；Tris, PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.50 Å R-free 0.401
2D10 Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide Deposited 2005-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 330–357（28 aa） Fragment：residues 331-358
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；10% PEG4000, 5% Isopropanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.268
2D10 Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide Deposited 2005-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 330–357（28 aa） Fragment：residues 331-358
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；10% PEG4000, 5% Isopropanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.268
2D10 Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide Deposited 2005-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 330–357（28 aa） Fragment：residues 331-358
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；10% PEG4000, 5% Isopropanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.268
2D10 Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide Deposited 2005-08-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 330–357（28 aa） Fragment：residues 331-358
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；10% PEG4000, 5% Isopropanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.50 Å R-free 0.268
2JXO Structure of the second PDZ domain of NHERF-1 Deposited 2007-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–240（91 aa） Fragment：PDZ 2 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；288.1 K；Ionic strength (raw mmCIF value) 0.15；Pressure ambient
NMR sample composition 1 mM PDZ2, HEPES, DTT, H2O, DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 1 mM [U-100% 15N] PDZ2, HEPES, DTT, H2O, DSS, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition 1 mM [U-100% 13C; U-100% 15N] PDZ2, HEPES, DTT, H2O, DSS, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KJD Solution structure of extended PDZ2 domain from NHERF1 (150-270) Deposited 2009-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–270（121 aa） Fragment：Extended PDZ2 domain: UNP residues 150-270
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；303 K；Ionic strength (raw mmCIF value) 0.150；Pressure ambient
NMR sample composition 550 uM [U-100% 13C; U-100% 15N] PDZ2-270-1, 20 mM HEPES-2, 0.5 mM DTT-3, 0.1 mM PMSF-4, 150 mM sodium chloride-5, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2KRG Solution Structure of human sodium/ hydrogen exchange regulatory factor 1(150-358) Deposited 2009-12-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–358（209 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；303 K；Pressure ambient
NMR sample composition 557 uM [U-100% 13C; U-100% 15N] NHERF1 (150-358), 20 mM HEPES, 150 mM sodium chloride, 0.5 mM DTT, 0.1 mM PMSF, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 389 uM [U-100% 13C; U-100% 15N; U-80% 2H] NHERF1 (150-358), 20 mM HEPES, 150 mM sodium chloride, 0.5 mM DTT, 0.1 mM PMSF, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 413 uM [U-100% 13C; U-100% 15N]-Leu,Val,Phe NHERF1 (150-358), 20 mM HEPES, 150 mM sodium chloride, 0.5 mM DTT, 0.1 mM PMSF, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2M0T Structural characterization of the extended PDZ1 domain from NHERF1 Deposited 2012-11-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–120（110 aa） Fragment：PDZ1 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；288 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition 400 uM [U-99% 13C; U-99% 15N] PDZ1, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2M0U Complex structure of C-terminal CFTR peptide and extended PDZ1 domain from NHERF1 Deposited 2012-11-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 11–120（110 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；288 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition 286 uM [U-99% 13C; U-99% 15N] PDZ1, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 275 uM CFTR, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2M0V Complex structure of C-terminal CFTR peptide and extended PDZ2 domain from NHERF1 Deposited 2012-11-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 150–270（121 aa） Fragment：PDZ2 domain
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；288 K；Ionic strength (raw mmCIF value) 150；Pressure ambient
NMR sample composition 400 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 688 uM CFTR, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition 200 uM [U-99% 13C; U-99% 15N] PDZ2, 20 mM TRIS, 150 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, 275 uM CFTR, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
2OZF The crystal structure of the 2nd PDZ domain of the human NHERF-1 (SLC9A3R1) Deposited 2007-02-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–235（86 aa） Fragment：Second PDZ domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.2M NaNO3, 0.1M BTProp, 20% PEG 3350, 10% Ethylene glycol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 1.50 Å R-free 0.211
4JL7 Crystal Structure of the Chemokine Receptor CXCR2 in Complex with the First PDZ Domain of NHERF1 Deposited 2013-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–95（85 aa） Fragment：unp residues 11-95
Not recorded CL CHLORIDE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；100 mM sodium acetate, 2.7 M sodium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.16 Å R-free 0.208
4LMM Crystal structure of NHERF1 PDZ1 domain complexed with the CXCR2 C-terminal tail in P21 space group Deposited 2013-07-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：UNP residues 11-95
Not recorded ACY ACETIC ACID × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；100 mM sodium acetate, 0.2 M ammonium acetate, 24% PEG4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.10 Å R-free 0.217
4MPA Crystal structure of NHERF1-CXCR2 signaling complex in P21 space group Deposited 2013-09-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：SEE REMARK 999
Not recorded ACY ACETIC ACID × 1 CL CHLORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；100 mM sodium acetate, pH 4.8, 0.2 M ammonium acetate, 24% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.10 Å R-free 0.157
4N6X Crystal Structure of the Chemokine Receptor CXCR2 in Complex with the First PDZ Domain of NHERF1 Deposited 2013-10-14 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：First PDZ Domain (UNP residues 11-94)
Not recorded CL CHLORIDE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；100 mM sodium acetate, pH 4.6, 2.7 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.05 Å R-free 0.163
4PQW Crystal Structure of Phospholipase C beta 3 in Complex with PDZ1 of NHERF1 Deposited 2014-03-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa） Fragment：unp residues 11-94
Not recorded CL CHLORIDE ION × 4 NI NICKEL (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；100 mM sodium acetate, pH 4.6, 2.5 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.47 Å R-free 0.196
4Q3H The crystal structure of NHERF1 PDZ2 CXCR2 complex revealed by the NHERF1 CXCR2 chimeric protein Deposited 2014-04-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 150–234（85 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；100 mM Tris HCl, pH 8.5, 8% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.44 Å R-free 0.195
4Q3H The crystal structure of NHERF1 PDZ2 CXCR2 complex revealed by the NHERF1 CXCR2 chimeric protein Deposited 2014-04-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 150–234（85 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；100 mM Tris HCl, pH 8.5, 8% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.44 Å R-free 0.195
6RQR Extended NHERF1 PDZ2 domain in complex with the PDZ-binding motif of CFTR Deposited 2019-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 150–269（120 aa）
Chain B 150–269（120 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；288 K；1.65 M ammonium sulphate, 100 mM tri-sodium citrate pH 5.6
Resolution 2.20 Å R-free 0.233
9RXQ Structure of the PDZ1 domain from human NHERF1 with the C-terminal residues (KSTQF) of human URAT1 transporter (SLC22A12) Deposited 2025-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；100 mM sodium acetate pH 4.8 200 mM ammonium acetate 24% PEG 4000
Resolution 1.38 Å R-free 0.183
9RXQ Structure of the PDZ1 domain from human NHERF1 with the C-terminal residues (KSTQF) of human URAT1 transporter (SLC22A12) Deposited 2025-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 11–94（84 aa）
Not recorded ACT ACETATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.4；293 K；100 mM sodium acetate pH 4.8 200 mM ammonium acetate 24% PEG 4000
Resolution 1.38 Å R-free 0.183
9RXT Structure of the PDZ1 domain from human NHERF1 with the C-terminal residues (NATRL) of the human sodium-dependent phosphate transporter 2A (NaPi-IIa, SLC34A1) Deposited 2025-07-11 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 11–94（84 aa）
Not recorded TOE 2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；100mM Bicine pH 9, 100mM NaCl, 30% PEG 550 MME
Resolution 1.36 Å R-free 0.198

21 other PDB entries and 26 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name O14745
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 2–85； UniProt 11–94

EZRIN-RADIXIN-MOESIN BINDING PHOSPHOPROTEIN-50

HOMO SAPIENS

UniProt P07550

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Insufficient information Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count Chain A； UniProt 409–413 Fragment：PDZ1 DOMAIN, RESIDUES 11-94 CL CHLORIDE ION × 4 X-RAY DIFFRACTION X-ray crystallization conditions：pH 4.24；CITRIC ACID, LITHIUM CHLORIDE, pH 4.24 Resolution 1.90 Å R-free 0.186

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1GQ4 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2R4R Crystal structure of the human beta2 adrenoceptor Deposited 2007-08-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–365（365 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 3.40 Å R-free 0.270
2R4S Crystal structure of the human beta2 adrenoceptor Deposited 2007-08-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 24–365（342 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；295 K；Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
Resolution 3.40 Å R-free 0.280
2RH1 High resolution crystal structure of human B2-adrenergic G protein-coupled receptor. Deposited 2007-10-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–365（103 aa）
Mutation：N187E, C54T, C97A Mutation：N187E, C54T, C97A SO4 SULFATE ION × 6 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC MESOPHASE；pH 6.75；293 K；30-35% v/v PEG 400, 0.1-0.2 M Na2SO4, 0.1 M Bis-tris propane pH 6.5-7.0, 5-7% 1,4-Butanediol, 8-10% Cholesterol, 52-50% Monoolein, pH 6.75, LIPIDIC MESOPHASE, temperature 293K
Resolution 2.40 Å R-free 0.232
3D4S Cholesterol bound form of human beta2 adrenergic receptor. Deposited 2008-05-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 CLR CHOLESTEROL × 2 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 X-RAY DIFFRACTION
X-ray crystallization conditions MESOPHASE；pH 7；293 K；28% v/v PEG 400, 300mM K formate, 100mM Bis-tris propane pH 7.0, 2mM Timolol, MESOPHASE, temperature 293K
Resolution 2.80 Å R-free 0.273
3KJ6 Crystal structure of a Methylated beta2 Adrenergic Receptor-Fab complex Deposited 2009-11-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 2–365（364 aa）
Mutation：N187E SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions bicelles, vapor diffusion；pH 7；298 K；AmSO4, pH 7, bicelles, vapor diffusion, temperature 298K
Resolution 3.40 Å R-free 0.280
3NY8 Crystal structure of the human beta2 adrenergic receptor in complex with the inverse agonist ICI 118,551 Deposited 2010-07-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A 263–348（86 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A CLR CHOLESTEROL × 2 JRZ (2S,3S)-1-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]butan-2-ol × 1 OLA OLEIC ACID × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 PGE TRIETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 293 K；100 mM Tris/HCl pH 7.5-8.0, 200-500 mM Na-formate, 5% 1,4 butanediol, 27-32% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
Resolution 2.84 Å R-free 0.291
3NY9 Crystal structure of the human beta2 adrenergic receptor in complex with a novel inverse agonist Deposited 2010-07-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A 263–348（86 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A CLR CHOLESTEROL × 2 JSZ ethyl 4-({(2S)-2-hydroxy-3-[(1-methylethyl)amino]propyl}oxy)-3-methyl-1-benzofuran-2-carboxylate × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 293 K；100 mM Tris/HCl pH 7.5-8, 5% 1,4 butanediol, 220 mM Na-formate, 27% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
Resolution 2.84 Å R-free 0.278
3NYA Crystal structure of the human beta2 adrenergic receptor in complex with the neutral antagonist alprenolol Deposited 2010-07-14 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Chain A 263–348（86 aa） Fragment：Chimeric protein of Beta-2 Adrenoreceptor 1-230, Lysozyme 2-161, Beta-2 adrenergic receptor 263-348
Mutation：E122W, N187E, C1054T, C1097A Mutation：E122W, N187E, C1054T, C1097A CLR CHOLESTEROL × 2 JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 293 K；100 mM Bis-Tris Propane pH 6.6-6.8, 120 mM Na-tartrate, 3% 1,3 butanediol, 25-30% PEG 400, Lipidic Cubic Phase (LCP) Crystallization, temperature 293-295K
Resolution 3.16 Å R-free 0.290
3P0G Structure of a nanobody-stabilized active state of the beta2 adrenoceptor Deposited 2010-09-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–230（230 aa） Fragment：UNP P07550 residues 1-230, 263-365, UNP P00720 residues 2-161
Chain A 263–365（103 aa） Fragment：UNP P07550 residues 1-230, 263-365, UNP P00720 residues 2-161
Mutation：N187E Mutation：N187E P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8；293 K；36-44% PEG 400, 100 mM Tris pH 8.0, 4% DMSO, 1% 1,2,3-heptanetriol, twin-syringe mixing method, temperature 293K
Resolution 3.50 Å R-free 0.308
3PDS Irreversible Agonist-Beta2 Adrenoceptor Complex Deposited 2010-10-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 25–230（206 aa）
Chain A 264–348（85 aa）
Mutation：H93C,N187E,C265A Mutation：H93C,N187E,C265A ERC 8-hydroxy-5-[(1R)-1-hydroxy-2-({2-[3-methoxy-4-(3-sulfanylpropoxy)phenyl]ethyl}amino)ethyl]quinolin-2(1H)-one × 1 CLR CHOLESTEROL × 1 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.7；293 K；26 %(v/v) PEG 400, 200 mM Li2SO4, 4 %(v/v) DMSO, 3.5 %(v/v) 1,4-butandediol, 100 mM MES pH 6.7, Lipidic Cubic Phase, temperature 293K
Resolution 3.50 Å R-free 0.283
3SN6 Crystal structure of the beta2 adrenergic receptor-Gs protein complex Deposited 2011-06-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 29–365（337 aa）
Mutation：C54T,C97A,M96T,M98T,N187E P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；293 K；350-450 mM potassium nitrate, 100 mM MES, 1 mM TCEP, 10 mM phosphonoformate, 0.01 mM BI167107, 18-22% PEG400. Crystals were grown in a 10:1 (w:w) MAG 7.7:cholesterol lipid mix. , pH 6.5, Lipidic cubic phase, temperature 293K
Resolution 3.20 Å R-free 0.277
4GBR N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor Deposited 2012-07-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–365（337 aa） Fragment：UNP RESIDUES 29-365
Mutation：M96T, M98T, N187E CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；293 K；PEG300 37% Bis-Tris propane 0.1M Ammonium phosphate, dibasic, 0.1M, pH 6.5, lipidic cubic phase, temperature 293K
Resolution 3.99 Å R-free 0.282
4LDE Structure of beta2 adrenoceptor bound to BI167107 and an engineered nanobody Deposited 2013-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–235（207 aa） Fragment：UNP residues 29-348 with a deletion of residues 235-263
Chain A 264–348（85 aa） Fragment：UNP residues 29-348 with a deletion of residues 235-263
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 NA SODIUM ION × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
Resolution 2.79 Å R-free 0.256
4LDL Structure of beta2 adrenoceptor bound to hydroxybenzylisoproterenol and an engineered nanobody Deposited 2013-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–235（207 aa）
Chain A 264–348（85 aa）
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A XQC 4-[(1R)-1-hydroxy-2-{[1-(4-hydroxyphenyl)-2-methylpropan-2-yl]amino}ethyl]benzene-1,2-diol × 1 NA SODIUM ION × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
Resolution 3.10 Å R-free 0.254
4LDO Structure of beta2 adrenoceptor bound to adrenaline and an engineered nanobody Deposited 2013-06-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–235（207 aa）
Chain A 264–348（85 aa）
Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A Mutation：C918T, C962A, M1096T, M1098T, N1157E, C1265A 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 ALE L-EPINEPHRINE × 1 NA SODIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 6.5；293 K；100 mM MES pH 6.2-6.7, 40-100 mM ammonium phosphate dibasic, 18-24% PEG400, LIPIDIC CUBIC PHASE, temperature 293K
Resolution 3.20 Å R-free 0.254
4QKX Structure of beta2 adrenoceptor bound to a covalent agonist and an engineered nanobody Deposited 2014-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–348（320 aa）
Mutation：C919T, C962A, H1093C, M1096T, M1098T, N1187E, C1265A NA SODIUM ION × 2 35V 4-[(1R)-1-hydroxy-2-({2-[3-methoxy-4-(2-sulfanylethoxy)phenyl]ethyl}amino)ethyl]benzene-1,2-diol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7.8；293 K；24-27 % PEG400, 100 mM Tris pH 7.8 to pH 8.4, 5-10 mM Sodium formate, lipidic cubic phase, temperature 293K
Resolution 3.30 Å R-free 0.280
5D5A In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–365（103 aa）
Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol
Resolution 2.48 Å R-free 0.262
5D5B In meso X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K Deposited 2015-08-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–365（103 aa）
Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; Mutation：;N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A ; CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 1 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 SO4 SULFATE ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol
Resolution 3.80 Å R-free 0.274
5D6L beta2AR-T4L - CIM Deposited 2015-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–223（223 aa）
Chain A 264–365（102 aa）
Mutation：N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A Mutation：N187E, C54T, C97A,N187E, C54T, C97A,N187E, C54T, C97A SO4 SULFATE ION × 6 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 2 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；30 - 35 % Peg 400, 5 -10 % butanediol, 0.1 - 0.35 mM Na sulphate, 0.1 M Bis-Tris pH 7.0.
Resolution 3.20 Å R-free 0.260
5JQH Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60 Deposited 2016-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 30–348（319 aa） Fragment：UNP RESIDUES 2-161, UNP RESIDEUS 30-348
Mutation：C919T, C962A, M1096T, M1098T, N1187E, C1265A,C919T, C962A, M1096T, M1098T, N1187E, C1265A CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 CLR CHOLESTEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7.5；293 K；100 mM HEPES pH 7.5, 20 mM EDTA, and 19-23% PEG300
Resolution 3.20 Å R-free 0.290
5JQH Structure of beta2 adrenoceptor bound to carazolol and inactive-state stabilizing nanobody, Nb60 Deposited 2016-05-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 30–348（319 aa） Fragment：UNP RESIDUES 2-161, UNP RESIDEUS 30-348
Mutation：C919T, C962A, M1096T, M1098T, N1187E, C1265A,C919T, C962A, M1096T, M1098T, N1187E, C1265A CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 CLR CHOLESTEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7.5；293 K；100 mM HEPES pH 7.5, 20 mM EDTA, and 19-23% PEG300
Resolution 3.20 Å R-free 0.290
5X7D Structure of beta2 adrenoceptor bound to carazolol and an intracellular allosteric antagonist Deposited 2017-02-25 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa） Fragment：UNP P07550 residues 1-230, UNP D9IEF7 residues 2-162, UNP P07550 residues 264-365
Chain A 264–365（102 aa） Fragment：UNP P07550 residues 1-230, UNP D9IEF7 residues 2-162, UNP P07550 residues 264-365
Mutation：N187E, C1054T, C1097A Mutation：N187E, C1054T, C1097A EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 CLR CHOLESTEROL × 2 8VS 4-carbamoyl-N-[(2R)-2-cyclohexyl-2-phenylacetyl]-L-phenylalanyl-3-bromo-N-methyl-L-phenylalaninamide × 1 CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 BU1 1,4-BUTANEDIOL × 1 ACM ACETAMIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7.5；293 K；0.1 M Tris-HCl, pH 7.5, 30% -37.5% PEG400, 400 mM NH4F, 6% 1,4-butanediol, 1 mM Cmpd-15PA and 1% DMSO.
Resolution 2.70 Å R-free 0.268
6E67 Structure of beta2 adrenergic receptor fused to a Gs peptide Deposited 2018-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–232（232 aa） Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Chain A 268–365（98 aa） Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7；293 K；100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol
Resolution 3.70 Å R-free 0.307
6E67 Structure of beta2 adrenergic receptor fused to a Gs peptide Deposited 2018-07-24 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–232（232 aa） Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Chain B 268–365（98 aa） Fragment：beta-2 (UNP residues 1-232), lysozyme (UNP residues 2-161), GS (UNP residues 1024-1036)
Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; Mutation：;M96T, M98T, N187E, C22A, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A,M96T, M98T, N187E, C1054T, C1097A ; P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7；293 K；100 mM Bis-Tris, pH 7.0, 75-125 mM ammonium acetate, 50-75 mM potassium fluoride, 38-42% PEG400, 6% ethylene glycol, 1.5% 1,2-propaneiol
Resolution 3.70 Å R-free 0.307
6MXT Crystal structure of human beta2 adrenergic receptor bound to salmeterol and Nb71 Deposited 2018-10-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–234（206 aa） Fragment：chimera of T4 lysozyme fused to human beta2 adrenergic receptor (UNP residues 29-234,263-365)
Chain A 263–365（103 aa） Fragment：chimera of T4 lysozyme fused to human beta2 adrenergic receptor (UNP residues 29-234,263-365)
Mutation：M96T, M98T, N187E Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：M96T, M98T, N187E Non-standard monomer：Yes (specific site not provided by mmCIF) K5Y salmeterol × 1 MG MAGNESIUM ION × 1 NI NICKEL (II) ION × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 HTO HEPTANE-1,2,3-TRIOL × 1 OLA OLEIC ACID × 1 P33 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 7.5；293.15 K；31-34% PEG400, 100 mM HEPES, pH 7.5, 1% 1,2,3-heptanetriol
Resolution 2.96 Å R-free 0.245
6N48 Structure of beta2 adrenergic receptor bound to BI167107, Nanobody 6B9, and a positive allosteric modulator Deposited 2018-11-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 29–234（206 aa） Fragment：chimera of lysozyme and B2AR (UNP residues 29-234,263-348)
Chain A 263–348（86 aa） Fragment：chimera of lysozyme and B2AR (UNP residues 29-234,263-348)
Mutation：M1096T, M1098T, N1187E Mutation：M1096T, M1098T, N1187E P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 1WV (2S)-2,3-dihydroxypropyl (7Z)-tetradec-7-enoate × 1 KBY N-[(3R)-4-(4-tert-butylphenyl)-3-({2-[(4-methoxyphenyl)sulfanyl]-5-[methyl(propan-2-yl)sulfamoyl]benzene-1-carbonyl}amino)butanoyl]glycine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 8；293 K；100 mM Tris-HCl, pH 8.0, 150-200 mM lithium acetate, 43-45% PEG400
Resolution 3.20 Å R-free 0.274
6NI3 B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex Deposited 2018-12-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 29–341（313 aa）
Not recorded P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6OBA The beta2 adrenergic receptor bound to a negative allosteric modulator Deposited 2019-03-20 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa） Fragment：beta-2 (UNP residues 1-230), lysozyme, beta-2 (UNP residues 264-365)
Chain A 264–365（102 aa） Fragment：beta-2 (UNP residues 1-230), lysozyme, beta-2 (UNP residues 264-365)
Mutation：N187E, C1054T, C1097A Mutation：N187E, C1054T, C1097A CLR CHOLESTEROL × 2 JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 M3J 6-bromo-N~2~-phenylquinazoline-2,4-diamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；pH 8；293 K；0.1 M Tris-HCl, pH 8.0, 30-40% PEG400, 300-400 mM sodium formate, 6% 1,4-butanediol, 1 mM alprenolol, 1 mM AS408, 1% DMSO
Resolution 3.10 Å R-free 0.277
6PRZ XFEL beta2 AR structure by ligand exchange from Alprenolol to Alprenolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 OLA OLEIC ACID × 2 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% (v/v) PEG 400, 2 mM Alprenolol
Resolution 2.80 Å R-free 0.228
6PS0 XFEL beta2 AR structure by ligand exchange from Alprenolol to Carazolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 OLA OLEIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Carazolol
Resolution 3.40 Å R-free 0.260
6PS1 XFEL beta2 AR structure by ligand exchange from Alprenolol to Timolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 2 OLA OLEIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Timolol
Resolution 3.20 Å R-free 0.264
6PS2 XFEL beta2 AR structure by ligand exchange from Timolol to Alprenolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 CLR CHOLESTEROL × 1 OLB (2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 3 SO4 SULFATE ION × 4 OLA OLEIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Alprenolol
Resolution 2.40 Å R-free 0.224
6PS3 XFEL beta2 AR structure by ligand exchange from Timolol to Carvedilol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded CVD (2S)-1-(8H-CARBAZOL-4-YLOXY)-3-[2-(2-METHOXYPHENOXY)ETHYLAMINO]PROPAN-2-OL × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 6 OLA OLEIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Carvedilol
Resolution 2.50 Å R-free 0.242
6PS4 XFEL beta2 AR structure by ligand exchange from Timolol to ICI-118551. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded JRZ (2S,3S)-1-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]-3-[(1-methylethyl)amino]butan-2-ol × 1 SO4 SULFATE ION × 3 CLR CHOLESTEROL × 1 OLA OLEIC ACID × 4 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 10 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand ICI-118551
Resolution 2.60 Å R-free 0.243
6PS5 XFEL beta2 AR structure by ligand exchange from Timolol to Propranolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded SNP 1-(ISOPROPYLAMINO)-3-(1-NAPHTHYLOXY)-2-PROPANOL × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLA OLEIC ACID × 2 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 4 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Propranolol
Resolution 2.90 Å R-free 0.258
6PS6 XFEL beta2 AR structure by ligand exchange from Timolol to Timolol. Deposited 2019-07-12 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–230（230 aa）
Chain A 263–348（86 aa）
Not recorded TIM (2S)-1-(tert-butylamino)-3-[(4-morpholin-4-yl-1,2,5-thiadiazol-3-yl)oxy]propan-2-ol × 1 SO4 SULFATE ION × 4 CLR CHOLESTEROL × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 OLA OLEIC ACID × 4 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；0.1 M HEPES pH 7.0, 0.1 M Ammonium Sulfate, 30% PEG 400, 2 mM of target ligand Timolol
Resolution 2.70 Å R-free 0.232
7DHI Cryo-EM structure of the partial agonist salbutamol-bound beta2 adrenergic receptor-Gs protein complex. Deposited 2020-11-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 1–348（348 aa）
Mutation：G16R,E27Q,C77V,M96T,M98T,E122W,C125V,N187E,C265A,C285A,C327S 68H SALBUTAMOL × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.26 Å
7DHR Cryo-EM structure of the full agonist isoprenaline-bound beta2 adrenergic receptor-Gs protein complex. Deposited 2020-11-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 1–348（348 aa）
Mutation：G16R,E27Q,C77V,M96T,M98T,E122W,C125V,N187E,C265A,C285A,C327S 5FW ISOPRENALINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7XK9 Structure of human beta2 adrenergic receptor bound to constrained isoproterenol Deposited 2022-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–235（207 aa）
Chain A 264–348（85 aa）
Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A GJ6 (5R,6R)-6-(propan-2-ylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；100mM Tris-HCl, pH 8.0, 150-200mM lithium acetate, 43-45% PEG 400
Resolution 3.40 Å R-free 0.261
7XKA Structure of human beta2 adrenergic receptor bound to constrained epinephrine Deposited 2022-04-19 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–235（207 aa）
Chain A 264–348（85 aa）
Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A Mutation：C918T,C962A,M1096T,M1098T,N1157E,C1265A G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 NA SODIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；100mM Tris-HCl, pH 8.0, 150-200mM lithium acetate, 43-45% PEG400
Resolution 3.10 Å R-free 0.272
8GDZ CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#1 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8GE1 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#2 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8GE2 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#3 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8GE3 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#4 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8GE4 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#5 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8GE5 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#6 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
8GE6 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#7 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8GE7 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#8 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8GE8 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#9 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
8GE9 CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#10 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8GEA CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#11 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8GEB CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#12 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8GEC CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#13 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8GED CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#14 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8GEE CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#15 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
8GEF CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#16 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8GEG CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#17 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8GEH CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#18 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
8GEI CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#19 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
8GEJ CryoEM structure of beta-2-adrenergic receptor in complex with nucleotide-free Gs heterotrimer (#20 of 20) Deposited 2023-03-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
8GFV CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.10 Å
8GFW CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GFX CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GFY CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GFZ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GG0 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 2.90 Å
8GG1 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GG2 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.00 Å
8GG3 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.10 Å
8GG4 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GG5 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GG6 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8GG7 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8GG8 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8GG9 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8GGA CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGB CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGC CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8GGE CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGF CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8GGI Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #1 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGJ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGK Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #3 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8GGL Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #4 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GGM Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #5 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GGN Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #6 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8GGO Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #7 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GGP Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #8 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.20 Å
8GGQ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #9 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8GGR Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #10 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8GGS Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #11 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGT Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #12 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGU Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #13 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8GGV Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #14 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8GGW Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #15 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8GGX Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #16 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8GGY Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #17 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.90 Å
8GGZ Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #18 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.90 Å
8GH0 Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #19 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 4.00 Å
8GH1 Locally refined cryoEM structure of receptor from beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #20 of 20) Deposited 2023-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain R 1–413（413 aa）
Not recorded G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 4.10 Å
8JJ8 Cryo-EM structure of the beta2AR-mBRIL/1b3 Fab/Glue complex with a partial agonist Deposited 2023-05-29 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain F 29–230（202 aa）
Chain F 263–340（78 aa）
Not recorded H98 ~{N}-[5-[(1~{R})-2-[[(2~{R})-1-(4-methoxyphenyl)propan-2-yl]amino]-1-oxidanyl-ethyl]-2-oxidanyl-phenyl]methanamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.20 Å
8JJL cryo-EM structure of the beta2-AR-mBRIL/1b3 Fab/Glue complex with a full agonist Deposited 2023-05-30 Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–230（202 aa）
Chain A 263–340（78 aa）
Not recorded DZQ Olodaterol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.20 Å
8JJO Cryo-EM structure of the beta2AR-mBRIL/1b3 Fab/Glue complex with an antagonist Deposited 2023-05-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain F 29–230（202 aa）
Chain F 263–340（78 aa）
Not recorded JTZ (2S)-1-[(1-methylethyl)amino]-3-(2-prop-2-en-1-ylphenoxy)propan-2-ol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen NITROGEN
Resolution 3.40 Å
8UHB Cryo-EM Structure of the Ro5256390-bound hTA1-Gs heterotrimer signaling complex Deposited 2023-10-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–30（30 aa）
Mutation：M(-129)W, R(-14)G, Q(-3)E, F112W WV8 (2R,4S)-4-[(2S)-2-phenylbutyl]-1,3-oxazolidin-2-amine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.35 Å
8UNL CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class A) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8UNM CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class B) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8UNN CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class C) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Combined datasets of complex plunge frozen at 5 sec, 10 sec, or 17 sec after GTP addition to the sample.
Resolution 3.40 Å
8UNO CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class D) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8UNP CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class E) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8UNQ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class F) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8UNR CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class G) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8UNS CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class H) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8UNT CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class I) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8UNU CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class J) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8UNV CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class K) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.30 Å
8UNW CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class L) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.40 Å
8UNX CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class M) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8UNY CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class N) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8UNZ CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class O) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.80 Å
8UO0 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class P) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8UO1 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class Q) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8UO2 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class R) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.60 Å
8UO3 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class S) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 3.50 Å
8UO4 CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (Class T) Deposited 2023-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–413（413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 G1I (5R,6R)-6-(methylamino)-5,6,7,8-tetrahydronaphthalene-1,2,5-triol × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
cryo-EM vitrification conditions Cryogen ETHANE；GTP was added just prior to freezing at 5 sec, 10 sec, or 17 sec before plunging.
Resolution 4.00 Å
8W1V The beta2 adrenergic receptor bound to a bitopic ligand Deposited 2024-02-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric
Chain A 1–365（365 aa）
Chain B 1–365（365 aa）
Mutation：N187E,C1054T,C1097A Mutation：N187E,C1054T,C1097A A1AE2 (2S)-1-[(3-{1-[4-(4-{(2S)-2-hydroxy-3-[(propan-2-yl)amino]propoxy}phenyl)butyl]-1H-1,2,3-triazol-4-yl}propyl)amino]-3-(2-propylphenoxy)propan-2-ol × 2 AV0 Lauryl Maltose Neopentyl Glycol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；293 K；100 mM Tris buffer (pH 8.0), 100 to 175 mM lithium sulfate, 38% to 42% PEG400, and 10 mM EDTA
Resolution 3.00 Å R-free 0.292
8ZBE cryo-EM structure of the octreotide-bound SSTR5-Gi complex Deposited 2024-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain A 1–24（24 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.24 Å
8ZCJ Cryo-EM structure of the pasireotide-bound SSTR5-Gi complex Deposited 2024-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain G 1–24（24 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
8ZWF cryoEM structure of JR14a bound C3aR-BRIL-BAG2 complex Deposited 2024-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–30（30 aa）
Mutation：E27Q,M29W,H124I A1D9A (2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-methyl-thiophen-2-yl]carbonylamino]pentanoic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
8ZWG cryoEM structure of JR14a bound C3aR-Gi complex Deposited 2024-06-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 1–30（30 aa）
Mutation：E27Q A1D9A (2~{S})-5-[bis(azanyl)methylideneamino]-2-[[5-[bis(4-chlorophenyl)methyl]-3-methyl-thiophen-2-yl]carbonylamino]pentanoic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9BUY Structure of the LM189-Bound beta2AR-Gi Complex Deposited 2024-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 1–413（413 aa）
Not recorded CLR CHOLESTEROL × 3 A1ASM 4-[(1R)-1-hydroxy-2-{[6-(4-phenylbutoxy)hexyl]amino}ethyl]benzene-1,2-diol × 1 KBY N-[(3R)-4-(4-tert-butylphenyl)-3-({2-[(4-methoxyphenyl)sulfanyl]-5-[methyl(propan-2-yl)sulfamoyl]benzene-1-carbonyl}amino)butanoyl]glycine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9CHU Cryo-EM structure of calcineurin fused beta2 adrenergic receptor in norepinephrine bound inactive state Deposited 2024-07-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 29–230（202 aa）
Chain A 263–336（74 aa）
Not recorded E5E Noradrenaline × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
9CHV cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in apo state Deposited 2024-07-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 29–230（202 aa）
Chain A 263–354（92 aa）
Not recorded FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.95 Å
9CHX cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in carazolol bound inactive state Deposited 2024-07-02 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 29–230（202 aa）
Chain A 263–354（92 aa）
Not recorded CAU (2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol × 1 FK5 8-DEETHYL-8-[BUT-3-ENYL]-ASCOMYCIN × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
9I52 Dopamine 1 receptor:GaS complex bound to 19B Deposited 2025-01-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–30（30 aa）
Not recorded A1IZU 6-(4-imidazo[1,2-a]pyridin-8-yl-2-methyl-phenyl)-1,5-dimethyl-pyrimidine-2,4-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9I54 Dopamine 1 receptor:GaS complex bound to 24 Deposited 2025-01-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 4 PDB declaration: tetrameric
Chain R 1–30（30 aa）
Not recorded A1IZV 6-(3-fluoranyl-5-furo[3,2-c]pyridin-4-yloxy-pyridin-2-yl)-1,5-dimethyl-pyrimidine-2,4-dione × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.72 Å
9L8L Structure of GPCR megacomplex bound to agonists Deposited 2024-12-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 1–413（413 aa）
Mutation：K237R,G276S,I277V DR7 (3S,8S,9S,12S)-3,12-BIS(1,1-DIMETHYLETHYL)-8-HYDROXY-4,11-DIOXO-9-(PHENYLMETHYL)-6-[[4-(2-PYRIDINYL)PHENYL]METHYL]-2,5, 6,10,13-PENTAAZATETRADECANEDIOIC ACID DIMETHYL ESTER × 1 P0G 8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2H-1,4-benzoxazin-3(4H)-one × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.22 Å
9LW5 Cryo-EM structure of dopamine bound mut-beta2-Adrenergic Receptor beta2R-M16-miniGs-Gbeta1gamma2-Nb35-scFv16 complex Deposited 2025-02-13 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 6 PDB declaration: hexameric
Chain R 1–348（348 aa）
Not recorded LDP L-DOPAMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.87 Å
9RKF Dark structure of beta-2 Adrenergic receptor with photoazolol in Dark state recorded at SwissFEL Deposited 2025-06-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 28–230（203 aa）
Chain A 264–342（79 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 5 CLR CHOLESTEROL × 3 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 5 1PE PENTAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 PLM PALMITIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；289 K；100mM tri-sodium citrate pH= 6.2, 245mM Li2SO4, 32% PEG 350 MME, 10uM photoazolol-1
Resolution 2.45 Å R-free 0.219
9RKG Mixed model refinement of beta-2 Adrenergic receptor with photoazolol in dark state and Light state, 10 seconds after light activation, recorded at SwissFEL Deposited 2025-06-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–230（202 aa）
Chain A 263–342（80 aa）
Not recorded SO4 SULFATE ION × 6 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 5 1PE PENTAETHYLENE GLYCOL × 3 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
Resolution 2.45 Å R-free 0.217
9RKH Dark structure of beta-2 Adrenergic receptor with photoazolol in Dark state recorded at LCLS Deposited 2025-06-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–230（202 aa）
Chain A 264–342（79 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 5 CLR CHOLESTEROL × 2 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 EDO 1,2-ETHANEDIOL × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
Resolution 2.50 Å R-free 0.218
9RKI Mixed model refinement of beta-2 Adrenergic receptor with photoazolol in dark state and Light state, 17 nanoseconds after light activation, recorded at LCLS Deposited 2025-06-13 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric
Chain A 29–230（202 aa）
Chain A 263–342（80 aa）
Not recorded SO4 SULFATE ION × 7 ACM ACETAMIDE × 1 CLR CHOLESTEROL × 3 PLM PALMITIC ACID × 1 12P DODECAETHYLENE GLYCOL × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 7 EDO 1,2-ETHANEDIOL × 2 GOL GLYCEROL × 1 1PE PENTAETHYLENE GLYCOL × 1 A1JHU ~{N}-[4-[(~{E})-[2-[(2~{S})-2-oxidanyl-3-(propan-2-ylamino)propoxy]phenyl]diazenyl]phenyl]ethanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIPIDIC CUBIC PHASE；289 K；100 mM tri-sodium citrate (pH= 6.2), 245 mM Li2SO4, 32% PEG 350 MME, 10 uM photoazolol-1
Resolution 2.60 Å R-free 0.234
9U4Y cryo-EM structure of Xenopus laevis GnRHR bound with mammal GnRH Deposited 2025-03-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric
Chain R 1–30（30 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.67 Å
9U9V beta2-adrenergic receptor in complex with methamphetamine Deposited 2025-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric
Chain R 1–348（348 aa）
Not recorded B40 (2S)-N-methyl-1-phenylpropan-2-amine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
9UAM CysLTR2-LTC4-miniGq complex Deposited 2025-04-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric
Chain A 2–30（29 aa）
Not recorded Y01 CHOLESTEROL HEMISUCCINATE × 1 LTX (5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-( 2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic acid × 1 CLR CHOLESTEROL × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9W3F Cryo-EM structure of the human beta2-adrenergic receptor in complex with a novel antagonist Deposited 2025-07-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–230（230 aa）
Chain A 264–365（102 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 3.04 Å

143 other PDB entries and 145 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name P07550
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 86–90； UniProt 409–413

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1gq4

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1gq4
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1gq4
Deposition date deposition_date2001-11-19
Structure title titleSTRUCTURAL DETERMINANTS OF THE NHERF INTERACTION WITH BETA2AR AND PDGFR
Keywords keywordsSIGNALING PROTEIN, PDZ, BETA2-ADRENERGIC RECEPTOR, PDGFR, NHERF, COMPLEX; SIGNALING PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier13.82 Å
Radius of gyration Rg (electron density) rg_electron12.36 Å
Forward intensity I(0) i02291670.00
Molecular weight molecular_weight9851.0 kDa
Excluded volume excluded_volume12177 ų
Envelope volume envelope_volume14345 ų
Hydration-shell volume shell_volume9993 ų
Envelope diameter envelope_diameter43.2 Å
Shell Rg shell_rg17.84 Å
Envelope Rg envelope_rg12.75 Å
Shape Rg shape_rg12.31 Å
Total Rg total_rg13.81 Å
Total atoms total_atoms686
Residues n_residues90
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax42.7 Å
Rg (real space) rg_real13.71 Å
Rg uncertainty (real space) rg_real_error0.23 Å
I(0) (real space) i0_real2.2920e+06
I(0) uncertainty (real space) i0_real_error2.2700e+04
Rg (reciprocal space) rg_reciprocal13.72 Å
I(0) (reciprocal space) i0_reciprocal2292000.0000
Solution quality estimate total_estimate0.6672
Solution quality rating solution_quality REASONABLE a REASONABLE solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary18.4 Å
Skewness Skewness skewness0.102
Kurtosis Kurtosis kurtosis-0.303
Angular range angular_range— – 0.5000 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha479300.0000
Real-space data points n_real_points80
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.861; Stabil: 1.000; Sysdev: 0.368; Positv: 1.000; Valcen: 0.985; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

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7. Fold Classification (SCOP + CATH) 3 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1gq4a1
Class classb — All beta proteins
Fold Fold foldb.36 — PDZ domain-like
Superfamily Superfamily superfamilyb.36.1 — PDZ domain-like
Family Family familyb.36.1.1 — PDZ domain
Domain ID domain_idd1gq4a2
Class classl — Artifacts
Fold Fold foldl.1 — Tags
Superfamily Superfamily superfamilyl.1.1 — Tags
Family Family familyl.1.1.1 — Tags

CATH v4.4 (1 domains)

Domain ID domain_id1gq4A00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology42 — Pdz3 Domain
Homologous superfamily homologous superfamily10 — PDZ domain
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8. Citations (3)

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9. Files and Curves (10)