8ghu

Methyltransferase RmtC bound to the 30S ribosomal subunit

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

16S rRNA (guanine(1405)-N(7))-methyltransferase

Escherichia coli

UniProt G4WZ44

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name G4WZ44_ECOLX
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–281; UniProt 15–295

30S ribosomal protein S3

OrganismNot specified

UniProt V0Y888

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name V0Y888_ECOLX
Isoform —
PDB entities 3
Chains and sequence ranges Author chain c; PDBConstruct 1–206; UniProt 2–207

30S ribosomal protein S4

OrganismNot specified

UniProt C3SR62

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR62_ECOLX
Isoform —
PDB entities 4
Chains and sequence ranges Author chain d; PDBConstruct 1–205; UniProt 2–206

30S ribosomal protein S5

OrganismNot specified

UniProt F4TL26

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name F4TL26_ECOLX
Isoform —
PDB entities 5
Chains and sequence ranges Author chain e; PDBConstruct 1–150; UniProt 10–159

30S ribosomal protein S6, non-modified isoform

OrganismNot specified

UniProt P02358

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RS6_ECOLI
Isoform —
PDB entities 6
Chains and sequence ranges Author chain f; PDBConstruct 1–100; UniProt 1–100

30S ribosomal protein S7

OrganismNot specified

UniProt P02359

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RS7_ECOLI
Isoform —
PDB entities 7
Chains and sequence ranges Author chain g; PDBConstruct 1–151; UniProt 2–152

30S ribosomal protein S8

OrganismNot specified

UniProt A0A828U358

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A828U358_ECOLX
Isoform —
PDB entities 8
Chains and sequence ranges Author chain h; PDBConstruct 1–129; UniProt 2–130

30S ribosomal protein S12

OrganismNot specified

UniProt I2UHF1

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name I2UHF1_ECOLX
Isoform —
PDB entities 9
Chains and sequence ranges Author chain l; PDBConstruct 1–123; UniProt 2–124

30S ribosomal protein S13

OrganismNot specified

UniProt C3SR52

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SR52_ECOLX
Isoform —
PDB entities 10
Chains and sequence ranges Author chain m; PDBConstruct 1–93; UniProt 2–94

30S ribosomal protein S15

OrganismNot specified

UniProt D7XN21

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name D7XN21_ECOLX
Isoform —
PDB entities 11
Chains and sequence ranges Author chain o; PDBConstruct 1–88; UniProt 2–89

30S ribosomal protein S16

OrganismNot specified

UniProt C3SYP2

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3SYP2_ECOLX
Isoform —
PDB entities 12
Chains and sequence ranges Author chain p; PDBConstruct 1–82; UniProt 1–82

30S ribosomal protein S17

OrganismNot specified

UniProt A0A080IK26

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S18 × 1 (W1EY92) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0A080IK26_ECOLX
Isoform —
PDB entities 13
Chains and sequence ranges Author chain q; PDBConstruct 1–80; UniProt 4–83

30S ribosomal protein S18

OrganismNot specified

UniProt W1EY92

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S20 × 1 (C3TRH7) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name W1EY92_ECOLX
Isoform —
PDB entities 14
Chains and sequence ranges Author chain r; PDBConstruct 1–55; UniProt 20–74

30S ribosomal protein S20

OrganismNot specified

UniProt C3TRH7

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 14 RNA 1 16S rRNA (guanine(1405)-N(7))-methyltransferase × 1 (G4WZ44) 16S rRNA × 1 30S ribosomal protein S3 × 1 (V0Y888) 30S ribosomal protein S4 × 1 (C3SR62) 30S ribosomal protein S5 × 1 (F4TL26) 30S ribosomal protein S6, non-modified isoform × 1 (P02358) 30S ribosomal protein S7 × 1 (P02359) 30S ribosomal protein S8 × 1 (A0A828U358) 30S ribosomal protein S12 × 1 (I2UHF1) 30S ribosomal protein S13 × 1 (C3SR52) 30S ribosomal protein S15 × 1 (D7XN21) 30S ribosomal protein S16 × 1 (C3SYP2) 30S ribosomal protein S17 × 1 (A0A080IK26) 30S ribosomal protein S18 × 1 (W1EY92) MAGNESIUM ION × 83 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name C3TRH7_ECOLX
Isoform —
PDB entities 15
Chains and sequence ranges Author chain t; PDBConstruct 1–85; UniProt 3–87

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8ghu
Deposition date deposition_date2023-03-12
Structure title titleMethyltransferase RmtC bound to the 30S ribosomal subunit
Keywords keywordsribosome, 30S, RmtC, 16S rRNA methyltransferase; RIBOSOME
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8ghu__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8ghu__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 1010 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8ghu__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)67.03 Å
Rg (electron density)66.89 Å
Total Rg66.95 Å
Atom count47408
Residues3359
Excluded volume723710 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8ghu__assembly_1__model_1 pentadecameric (15) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (16)

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6. Fold Classification (SCOP + CATH) 3 domains

CATH v4.4 (3 domains)

Domain ID domain_id8ghud01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology1050 — Ribosomal Protein S4 Delta 41; Chain A, domain 1
Homologous superfamily homologous superfamily10 — Ribosomal protein S4/S9, N-terminal domain
Domain ID domain_id8ghud02
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology290 — Structural Genomics Hypothetical 15.5 Kd Protein In mrcA-pckA Intergenic Region; Chain A
Homologous superfamily homologous superfamily10 — RNA-binding S4 domain
Domain ID domain_id8ghuh01
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology1370 — Ribosomal Protein S8; Chain: A, domain 1
Homologous superfamily homologous superfamily30 —
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7. Citations (1)