8irh

XFEL structure of cyanobacterial photosystem II following two flashes (2F) with a 200-microsecond delay

Method: X-RAY DIFFRACTION

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Photosystem II protein D1

Thermostichus vulcanus

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBA_THEVL
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–344； UniProt 1–344 Author chain a； PDBConstruct 1–344； UniProt 1–344

Photosystem II CP47 reaction center protein

Thermostichus vulcanus

UniProt D0VWR1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBB_THEVL
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–505； UniProt 1–505 Author chain b； PDBConstruct 1–505； UniProt 1–505

Photosystem II CP43 reaction center protein

Thermostichus vulcanus

UniProt D0VWR7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBC_THEVL
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 5–455； UniProt 1–451 Author chain c； PDBConstruct 5–455； UniProt 1–451

Photosystem II D2 protein

Thermostichus vulcanus

UniProt D0VWR8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBD_THEVL
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–342； UniProt 1–342 Author chain d； PDBConstruct 1–342； UniProt 1–342

Cytochrome b559 subunit alpha

Thermostichus vulcanus

UniProt P12238

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBE_THEVL
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–84； UniProt 1–84 Author chain e； PDBConstruct 1–84； UniProt 1–84

Cytochrome b559 subunit beta

Thermostichus vulcanus

UniProt P12239

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBF_THEVL
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–44； UniProt 2–45 Author chain f； PDBConstruct 1–44； UniProt 2–45

Photosystem II reaction center protein H

Thermostichus vulcanus

UniProt P19052

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBH_THEVL
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain H； PDBConstruct 1–65； UniProt 1–65 Author chain h； PDBConstruct 1–65； UniProt 1–65

Photosystem II reaction center protein I

Thermostichus vulcanus

UniProt P12240

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBI_THEVL
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain I； PDBConstruct 1–38； UniProt 1–38 Author chain i； PDBConstruct 1–38； UniProt 1–38

Photosystem II reaction center protein J

Thermostichus vulcanus

UniProt Q7DGD4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBJ_THEVL
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain J； PDBConstruct 1–39； UniProt 2–40 Author chain j； PDBConstruct 1–39； UniProt 2–40

Photosystem II reaction center protein K

Thermostichus vulcanus

UniProt P19054

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBK_THEVL
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain K； PDBConstruct 1–37； UniProt 1–37 Author chain k； PDBConstruct 1–37； UniProt 1–37

Photosystem II reaction center protein L

Thermostichus vulcanus

UniProt P12241

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBL_THEVL
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain L； PDBConstruct 1–37； UniProt 1–37 Author chain l； PDBConstruct 1–37； UniProt 1–37

Photosystem II reaction center protein M

Thermostichus vulcanus

UniProt P12312

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBM_THEVL
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain M； PDBConstruct 1–36； UniProt 1–36 Author chain m； PDBConstruct 1–36； UniProt 1–36

Photosystem II manganese-stabilizing polypeptide

Thermostichus vulcanus

UniProt D0VWR2

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBO_THEVL
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain O； PDBConstruct 1–244； UniProt 1–244 Author chain o； PDBConstruct 1–244； UniProt 1–244

Photosystem II reaction center protein T

Thermostichus vulcanus

UniProt P12313

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBT_THEVL
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain T； PDBConstruct 1–32； UniProt 1–32 Author chain t； PDBConstruct 1–32； UniProt 1–32

Photosystem II 12 kDa extrinsic protein

Thermostichus vulcanus

UniProt P56152

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBU_THEVL
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain U； PDBConstruct 1–104； UniProt 1–104 Author chain u； PDBConstruct 1–104； UniProt 1–104

Cytochrome c-550

Thermostichus vulcanus

UniProt P0A387

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CY550_THEVL
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain V； PDBConstruct 1–137； UniProt 27–163 Author chain v； PDBConstruct 1–137； UniProt 27–163

Photosystem II reaction center protein X

Thermostichus vulcanus

UniProt D0VWR4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBX_THEVL
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain X； PDBConstruct 1–40； UniProt 1–40 Author chain x； PDBConstruct 1–40； UniProt 1–40

Photosystem II reaction center protein Ycf12

Thermostichus vulcanus

UniProt D0VWR3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Z × 2 （D0VWR5） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	YCF12_THEVL
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain Y； PDBConstruct 1–30； UniProt 1–30 Author chain y； PDBConstruct 1–30； UniProt 1–30

Photosystem II reaction center protein Z

Thermostichus vulcanus

UniProt D0VWR5

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II protein Y × 1 （P0DM37） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBZ_THEVL
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain Z； PDBConstruct 1–62； UniProt 1–62 Author chain z； PDBConstruct 1–62； UniProt 1–62

Photosystem II protein Y

Thermostichus vulcanus

UniProt P0DM37

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 39	Photosystem II protein D1 × 2 （P51765） Photosystem II CP47 reaction center protein × 2 （D0VWR1） Photosystem II CP43 reaction center protein × 2 （D0VWR7） Photosystem II D2 protein × 2 （D0VWR8） Cytochrome b559 subunit alpha × 2 （P12238） Cytochrome b559 subunit beta × 2 （P12239） Photosystem II reaction center protein H × 2 （P19052） Photosystem II reaction center protein I × 2 （P12240） Photosystem II reaction center protein J × 2 （Q7DGD4） Photosystem II reaction center protein K × 2 （P19054） Photosystem II reaction center protein L × 2 （P12241） Photosystem II reaction center protein M × 2 （P12312） Photosystem II manganese-stabilizing polypeptide × 2 （D0VWR2） Photosystem II reaction center protein T × 2 （P12313） Photosystem II 12 kDa extrinsic protein × 2 （P56152） Cytochrome c-550 × 2 （P0A387） Photosystem II reaction center protein X × 2 （D0VWR4） Photosystem II reaction center protein Ycf12 × 2 （D0VWR3） Photosystem II reaction center protein Z × 2 （D0VWR5） CA-MN4-O6 CLUSTER × 2 FE (II) ION × 2 CHLORIDE ION × 4 CHLOROPHYLL A × 70 PHEOPHYTIN A × 4 BETA-CAROTENE × 22 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL × 8 GLYCEROL × 21 CA-MN4-O5 CLUSTER × 2 ;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ; × 4 UNKNOWN LIGAND × 18 DODECYL-BETA-D-MALTOSIDE × 14 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE × 10 heptyl 1-thio-beta-D-glucopyranoside × 11 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE × 12 DIGALACTOSYL DIACYL GLYCEROL (DGDG) × 8 CALCIUM ION × 7 BICARBONATE ION × 2 PROTOPORPHYRIN IX CONTAINING FE × 2 MAGNESIUM ION × 2 HEME C × 2 water × 35	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSBY_THEVL
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain R； PDBConstruct 1–34； UniProt 1–34

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	8irh
Deposition date deposition_date	2023-03-17
Structure title title	XFEL structure of cyanobacterial photosystem II following two flashes (2F) with a 200-microsecond delay
Keywords keywords	PSII, photosystem II, XFEL, PHOTOSYNTHESIS; PHOTOSYNTHESIS
Experimental Method method	X-RAY DIFFRACTION

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8irh__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8irh__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

8irh__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	57.80 Å
Rg (electron density)	57.22 Å
Total Rg	57.53 Å
Atom count	50810
Residues	5283
Excluded volume	943760 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`8irh__assembly_1__model_1`	39-meric （39）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (42)

Entity 1 Entity 1 polymer

Description description	Photosystem II protein D1
Molecular weight formula_weight	38235.6 kDa
EC 编号 ec_number	1.10.3.9
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,a
Atom count atom_count	62605

MTTTLQRRESANLWERFCNWVTSTDNRLYVGWFGVIMIPTLLAATICFVIAFIAAPPVDIDGIREPVSGSLLYGNNIITGAVVPSSNAIGLHFYPIWEAASLDEWLYNGGPYQLIIFHFLLGASCYMGRQWELSYRLGMRPWICVAYSAPLASAFAVFLIYPIGQGSFSDGMPLGISGTFNFMIVFQAEHNILMHPFHQLGVAGVFGGALFCAMHGSLVTSSLIRETTETESANYGYKFGQEEETYNIVAAHGYFGRLIFQYASFNNSRSLHFFLAAWPVVGVWFAALGISTMAFNLNGFNFNHSVIDAKGNVINTWADIINRANLGMEVMHERNAHNFPLDLA

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 2 Entity 2 polymer

Description description	Photosystem II CP47 reaction center protein
Molecular weight formula_weight	56068.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B,b

GLPWYRVHTVLINDPGRLIAAHLMHTALVAGWAGSMALYELATFDPSDPVLNPMWRQGMFVLPFMARLGVTGSWSGWSITGETGIDPGFWSFEGVALAHIVLSGLLFLAACWHWVYWDLELFRDPRTGEPALDLPKMFGIHLFLAGLLCFGFGAFHLTGLFGPGMWVSDPYGLTGSVQPVAPEWGPDGFNPYNPGGVVAHHIAAGIVGIIAGLFHILVRPPQRLYKALRMGNIETVLSSSIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDSSYFQQEINRRVQASLASGATLEEAWSAIPEKLAFYDYIGNNPAKGGLFRTGPMNKGDGIAQAWKGHAVFRNKEGEELFVRRMPAFFESFPVILTDKNGVVKADIPFRRAESKYSFEQQGVTVSFYGGELNGQTFTDPPTVKSYARKAIFGEIFEFDTETLNSDGIFRTSPRGWFTFAHAVFALLFFFGHIWHGARTLFRDVFSGIDPELSPEQVEWGFYQKVGDVTTRK

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 3 Entity 3 polymer

Description description	Photosystem II CP43 reaction center protein
Molecular weight formula_weight	49668.8 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C,c

NSIFATNRDQESSGFAWWAGNARLINLSGKLLGAHVAHAGLIVFWAGAMTLFELAHFIPEKPMYEQGLILIPHIATLGWGVGPGGEVVDTFPFFVVGVVHLISSAVLGFGGVYHAIRGPETLEEYSSFFGYDWKDKNKMTTILGFHLIVLGIGALLLVAKAMFFGGLYDTWAPGGGDVRVITNPTLDPRVIFGYLLKSPFGGEGWIVSVNNLEDVVGGHIWIGLICIAGGIWHILTTPFGWARRAFIWSGEAYLSYSLGALSMMGFIATCFVWFNNTVYPSEFYGPTGPEASQAQAMTFLIRDQKLGANVGSAQGPTGLGKYLMRSPTGEIIFGGETMRFWDFRGPWLEPLRGPNGLDLNKIKNDIQPWQERRAAEYMTHAPLGSLNSVGGVATEINSVNFVSPRSWLATSHFVLAFFFLVGHLWHAGRARAAAAGFEKGIDRESEPVLSMPSLD

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 4 Entity 4 polymer

Description description	Photosystem II D2 protein
Molecular weight formula_weight	38404.9 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,d

ERGWFDILDDWLKRDRFVFVGWSGILLFPCAYLALGGWLTGTTFVTSWYTHGLASSYLEGCNFLTVAVSTPANSMGHSLLLLWGPEAQGDFTRWCQLGGLWTFIALHGAFGLIGFMLRQFEIARLVGVRPYNAIAFSAPIAVFVSVFLIYPLGQSSWFFAPSFGVAAIFRFLLFFQGFHNWTLNPFHMMGVAGVLGGALLCAIHGATVENTLFQDGEGASTFRAFNPTQAEETYSMVTANRFWSQIFGIAFSNKRWLHFFMLFVPVTGLWMSAIGVVGLALNLRSYDFISQEIRAAEDPEFETFYTKNLLLNEGIRAWMAPQDQPHENFVFPEEVLPRGNAL

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 5 Entity 5 polymer

Description description	Cytochrome b559 subunit alpha
Molecular weight formula_weight	9580.8 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E,e

MAGTTGERPFSDIITSVRYWVIHSITIPALFIAGWLFVSTGLAYDVFGTPRPDSYYAQEQRSIPLVTDRFEAKQQVETFLEQLK

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 6 Entity 6 polymer

Description description	Cytochrome b559 subunit beta
Molecular weight formula_weight	4936.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F,f

TSNTPNQEPVSYPIFTVRWVAVHTLAVPTIFFLGAIAAMQFIQR

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 7 Entity 7 polymer

Description description	Photosystem II reaction center protein H
Molecular weight formula_weight	7227.6 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,h

ARRTWLGDILRPLNSEYGKVAPGWGTTPLMAVFMGLFLVFLLIILEIYNSTLILDGVNVSWKALG

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 8 Entity 8 polymer

Description description	Photosystem II reaction center protein I
Molecular weight formula_weight	4438.3 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I,i

METLKITVYIVVTFFVLLFVFGFLSGDPARNPKRKDLE

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 9 Entity 9 polymer

Description description	Photosystem II reaction center protein J
Molecular weight formula_weight	3974.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J,j

MSEGGRIPLWIVATVAGMGVIVIVGLFFYGAYAGLGSSL

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 10 Entity 10 polymer

Description description	Photosystem II reaction center protein K
Molecular weight formula_weight	4101.9 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K,k

KLPEAYAIFDPLVDVLPVIPVLFLALAFVWQAAVGFR

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 11 Entity 11 polymer

Description description	Photosystem II reaction center protein L
Molecular weight formula_weight	4299.0 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L,l

MEPNPNRQPVELNRTSLYLGLLLILVLALLFSSYFFN

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 12 Entity 12 polymer

Description description	Photosystem II reaction center protein M
Molecular weight formula_weight	4009.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M,m

MEVNQLGLIATALFVLVPSVFLIILYVQTESQQKSS

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 13 Entity 13 polymer

Description description	Photosystem II manganese-stabilizing polypeptide
Molecular weight formula_weight	26651.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O,o

QTLTYDDIVGTGLANKCPTLDDTARGAYPIDSSQTYRIARLCLQPTTFLVKEEPKNKRQEAEFVPTKLVTRETTSLDQIQGELKVNSDGSLTFVEEDGIDFQPVTVQMAGGERIPLLFTVKNLVASTQPNVTSITTSTDFKGEFNVPSYRTANFLDPKGRGLASGYDSAIALPQAKEEELARANVKRFSLTKGQISLNVAKVDGRTGEIAGTFESEQLSDDDMGAHEPHEVKIQGVFYASIEPA

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 14 Entity 14 polymer

Description description	Photosystem II reaction center protein T
Molecular weight formula_weight	3906.7 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T,t

METITYVFIFACIIALFFFAIFFREPPRITKK

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 15 Entity 15 polymer

Description description	Photosystem II 12 kDa extrinsic protein
Molecular weight formula_weight	11656.0 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U,u

ATASTEEELVNVVDEKLGTAYGEKIDLNNTNIAAFIQYRGLYPTLAKLIVKNAPYESVEDVLNIPGLTERQKQILRENLEHFTVTEVETALVEGGDRYNNGLYK

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 16 Entity 16 polymer

Description description	Cytochrome c-550
Molecular weight formula_weight	15148.3 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V,v

AELTPEVLTVPLNSEGKTITLTEKQYLEGKRLFQYACASCHVGGITKTNPSLDLRTETLALATPPRDNIEGLVDYMKNPTTYDGEQEIAEVHPSLRSADIFPKMRNLTEKDLVAIAGHILVEPKILGDKWGGGKVYY

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 17 Entity 17 polymer

Description description	Photosystem II reaction center protein X
Molecular weight formula_weight	4191.0 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X,x

TITPSLKGFFIGLLSGAVVLGLTFAVLIAISQIDKVQRSL

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 18 Entity 18 polymer

Description description	Photosystem II reaction center protein Ycf12
Molecular weight formula_weight	3228.0 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y,y

EVIAQLTMIAMIGIAGPMIIFLLAVRRGNL

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 19 Entity 19 polymer

Description description	Photosystem II reaction center protein Z
Molecular weight formula_weight	6766.2 kDa
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z,z

MTILFQLALAALVILSFVMVIGVPVAYASPQDWDRSKQLIFLGSGLWIALVLVVGVLNFFVV

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 20 Entity 20 polymer

Description description	Photosystem II protein Y
Molecular weight formula_weight	3859.7 kDa
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

DWRVLVVLLPVLLAAGWAVRNILPYAVKQVQKLL

Source Source

Source organism organism	Thermostichus vulcanus
NCBI taxonomy ID ncbi_taxonomy_id	32053
Host organism host_organism	Thermostichus vulcanus

Entity 21 Entity 21 non-polymer

Description description	CA-MN4-O6 CLUSTER
Molecular weight formula_weight	355.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 22 Entity 22 non-polymer

Description description	FE (II) ION
Molecular weight formula_weight	55.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 23 Entity 23 non-polymer

Description description	CHLORIDE ION
Molecular weight formula_weight	35.5 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 24 Entity 24 non-polymer

Description description	CHLOROPHYLL A
Molecular weight formula_weight	893.5 kDa
Source method src_method	syn
Copy count entity_count	70

Entity 25 Entity 25 non-polymer

Description description	PHEOPHYTIN A
Molecular weight formula_weight	871.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 26 Entity 26 non-polymer

Description description	BETA-CAROTENE
Molecular weight formula_weight	536.9 kDa
Source method src_method	syn
Copy count entity_count	22

Entity 27 Entity 27 non-polymer

Description description	1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
Molecular weight formula_weight	795.1 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 28 Entity 28 non-polymer

Description description	GLYCEROL
Molecular weight formula_weight	92.1 kDa
Source method src_method	syn
Copy count entity_count	21

Entity 29 Entity 29 non-polymer

Description description	CA-MN4-O5 CLUSTER
Molecular weight formula_weight	339.8 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 30 Entity 30 non-polymer

Description description	;2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE ;
Molecular weight formula_weight	749.2 kDa
Source method src_method	syn
Copy count entity_count	4

Entity 31 Entity 31 non-polymer

Description description	UNKNOWN LIGAND
Molecular weight formula_weight	510.6 kDa
Source method src_method	syn
Copy count entity_count	18

Entity 32 Entity 32 non-polymer

Description description	DODECYL-BETA-D-MALTOSIDE
Molecular weight formula_weight	510.6 kDa
Source method src_method	syn
Copy count entity_count	14

Entity 33 Entity 33 non-polymer

Description description	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
Molecular weight formula_weight	723.0 kDa
Source method src_method	syn
Copy count entity_count	10

Entity 34 Entity 34 non-polymer

Description description	heptyl 1-thio-beta-D-glucopyranoside
Molecular weight formula_weight	294.4 kDa
Source method src_method	syn
Copy count entity_count	11

Entity 35 Entity 35 non-polymer

Description description	1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
Molecular weight formula_weight	787.2 kDa
Source method src_method	syn
Copy count entity_count	12

Entity 36 Entity 36 non-polymer

Description description	DIGALACTOSYL DIACYL GLYCEROL (DGDG)
Molecular weight formula_weight	949.3 kDa
Source method src_method	syn
Copy count entity_count	8

Entity 37 Entity 37 non-polymer

Description description	CALCIUM ION
Molecular weight formula_weight	40.1 kDa
Source method src_method	syn
Copy count entity_count	7

Entity 38 Entity 38 non-polymer

Description description	BICARBONATE ION
Molecular weight formula_weight	61.0 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 39 Entity 39 non-polymer

Description description	PROTOPORPHYRIN IX CONTAINING FE
Molecular weight formula_weight	616.5 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 40 Entity 40 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 41 Entity 41 non-polymer

Description description	HEME C
Molecular weight formula_weight	618.5 kDa
Source method src_method	nat
Copy count entity_count	2

Entity 42 Entity 42 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	1826

▶

Unit-cell length a length_a	125.770 Å
Unit-cell length b length_b	231.760 Å
Unit-cell length c length_c	288.580 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.250 Å
Low-resolution limit resolution_low	19.990 Å
R factor r_factor	0.1449
R_work r_work	0.1428
R_free r_free	0.1832

XFEL structure of cyanobacterial photosystem II following two flashes (2F) with a 200-microsecond delay

1. Protein Identity and Related Structures Protein Identity & Related Structures

Photosystem II protein D1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II CP47 reaction center protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II CP43 reaction center protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II D2 protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cytochrome b559 subunit alpha

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cytochrome b559 subunit beta

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein H

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein I

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein J

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Photosystem II reaction center protein K

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer