8p8b

Mycoplasma pneumoniae large ribosomal subunit in chloramphenicol-treated cells

Method: ELECTRON MICROSCOPY D_max: 268.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	8p8b
沉积日期 deposition_date	2023-05-31
结构标题 title	Mycoplasma pneumoniae large ribosomal subunit in chloramphenicol-treated cells
关键词 keywords	In situ, Ribosome, Chloramphenicol, cryo-ET, TRANSLATION; TRANSLATION
实验方法 method	ELECTRON MICROSCOPY

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	71.42 Å
回转半径 Rg (电子) rg_electron	72.59 Å
零角强度 I(0) i0	68951700000.00
分子量 molecular_weight	1448700.0 kDa
排除体积 excluded_volume	1486600 Å³
包络体积 envelope_volume	2553500 Å³
水化壳体积 shell_volume	268380 Å³
包络直径 envelope_diameter	245.1 Å
壳层 Rg shell_rg	85.61 Å
包络 Rg envelope_rg	72.40 Å
形状 Rg shape_rg	72.66 Å
总 Rg total_rg	72.53 Å
总原子数 total_atoms	97182
残基数 n_residues	6768
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	268.8 Å
Rg (实空间) rg_real	74.63 Å
Rg 误差 (实空间) rg_real_error	1.57 Å
I(0) (实空间) i0_real	6.9020e+10
I(0) 误差 (实空间) i0_real_error	1.4370e+09
Rg (倒空间) rg_reciprocal	72.13 Å
I(0) (倒空间) i0_reciprocal	69070000000.0000
解质量估计 total_estimate	0.8707
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	85.5 Å
偏度 Skewness skewness	0.581
峰度 Kurtosis kurtosis	0.528
角度范围 angular_range	— – 0.1100 Å⁻¹
当前正则化参数 α current_alpha	0.9415
最高正则化参数 α highest_alpha	10070000000.0000
实空间数据点数 n_real_points	23
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.602; Stabil: 0.891; Sysdev: 1.000; Positv: 1.000; Valcen: 0.936; Smooth: 0.919

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (47)

Entity 1 polymer

描述 description	50S ribosomal protein L34
分子量 formula_weight	5616.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	0
原子数 atom_count	97182

MKRTYQPSKLKRAKTHGFLARMATASGRKVLKLRRKKQRAQLTVSSER

Entity 2 polymer

描述 description	50S ribosomal protein L35
分子量 formula_weight	6793.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	1

MKVKSAAKKRFKLTKSGQIKRKHAYTSHLAPHKTTKQKRHLRKQGTVSASDFKRIGNLI

Entity 3 polymer

描述 description	50S ribosomal protein L36
分子量 formula_weight	4400.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	2

MKVRASVKPICKDCKIIKRHQIVRVICKTQKHKQRQG

Entity 4 polymer

描述 description	23S ribosomal RNA
分子量 formula_weight	940953.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	3

UACAAUAAGUUACUAAGGGCUUAUGGUGGAUGCCUUGGCACUAAUAGGCGAUGAAGGACGUGUUAACCUGCGAUAAGCUUCGGGUAGGUGGUAAGAACCUCAGAUCCGGAGAUUUCCGAAUGGAGCAAUCCGGUAGUUGGAAACAGCUAUCAUUAAUUGAUGAAUAAAUAGUCAAUUAAAGCAAUACGUGGUGAAGUGAAACAUCUCAGUAGCCACAGGAAAAGAAAACGAAUGUGAUUCCGUGUGUAGUGGCGAGCGAAAGCGGAACAGGCCAAACUUAUCAUUAGAUAGGGGUUGUAGGGCUUGCAAUGUGGACUUGAAAACGAUAGAAGAAGCUGUUGGAAAGCAGCGCGCAAAAGGGUGAUAGCCCCGUAUUUGAAAUUGUUUUCAUACCUAGCGAGAUCCCUGAGUAGCUCGGAAAACGUUAUUUUGAGUGAAUCUGCCCAGACCAUUGGGUAAGCCUAAAUACUAAUUAGUGACCGAUAGCGAAACAGUACCGUGAGGGAAAGGUGAAAAGAACCCAGAGAUGGGAGUGAAAUAGAUUCUGAAACCAUAUGCCUACAACGUGUCAGAGCACAUUAAUGUGUGAUGGCGUGCGUUUUGAAGUAUGAGCCGGCGAGUUAUGAUAGCAAGCGUUAGUUAACCAGGAGAUGGGGAGCUGUAGCGAAAGCGAGUUUUAAAAGAGCGUUUGUUUGUUAUUAUAGACCCGAAACGGGUUGAGCUAGUCAUGAGCAGGUUGAAGGUUGAGUAACAUCAACUGGAGGACCGAACCGACUCUCGUUGAAACGAUAGCGGAUGACUUGUGAUUAGGGGUGAAAUUCCAAUCGAAAUCCGUGAUAGCUGGUUCUCGUCGAAAUAGCUUUAAGGCUAGCGUGAGAUCACAAAUAAGUGGAGGUAAAGCUACUGAAUGUAUGAUGGCGCCACCUAGGCGUACUGAAUACAAUUAAACUCUGAAUGCCAUUUAUUUUAUUCUCGCAGUCAGACAGUGGGGGAUAAGCUUCAUUGUCAAGAGGGGAAGAGCCCAGAUCAUUAAAUAAGGUCCCCAAAAUAUACUAAGUGGAAAAGGAUGUGAAAGUGCUAAAACAGCAAGGAUGUUGGCUUAGAAGCAGCCAUCGUUUAAAGAGUGCGUAACAGCUCACUUGUCGAGUGUUUUUGCGCCGAAGAUGUAACGGGGCUAAGUAUAUUACCGAAUUUAUGGAUAAGAUUUAUAUCUUGUGGUAGACGAGCGUUGUAUUGGAGUUGAAGUCAAAGCGUGAGCAUUGGUGGAUCCAAUACAAGUGAGAAUGCCGGCAUGAGUAACGCUUGGGAGUGAGAAUCUCCCAAACCGAUUGACUAAGGUUUCCUGGACCAGGGUCGUCCUUCCAGGGUUAGUCUGGACCUAAGCUGAGGCUGAAAAGCGUAGGCGAUGGACAACAGGUUAAUAUUCCUGUACUUACAGUUAGACUGAUGGAGUGACAAAGAAGGUUUUCCACCCCCAUAAUUGGAUUUGGGGAUAAAUCAUAAGGUGGUACAAUAGGCAAAUCCGUUGUGCAUAACAUUGAGUGAUGAUGUCGAGUGAAUGAGUGAUCAAGUAGCGAAGGUGGUAUUAAUCAUGCUUUCAAGAAAAGCUUCUAGGGUUAAUCUAGCUGUAACCAGUACCGAGAACGAACACACGUAGUCAAGGAGAGGAUCCUAAGGUUAGCGAGUGAACUAUAGCCAAGGAACUCUGCAAAUUAACCCCGUAAGUUAGCGAGAAGGGGUGCUUAUGUAAAAGUAAGCCGCAGUGAAGAACGAGGGGGGACUGUUUAACUAAAACACAACUCUAUGCCAAACCGUAAGGUGAUGUAUAUGGGGUGACACCUGCCCAGUGCUGGAAGGUUAAAGAAGGAGGUUAGCGCAAGCGAAGCUUUUAACUGAAGCCCCAGUGAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUAGUCGGGUAAAUUCCGUCCCGCUUGAAUGGUGUAACCAUCUCUUGACUGUCUCGGCUAUAGACUCGGUGAAAUCCAGGUACGGGUGAAGACACCCGUUAGGCGCAACGGGACGGAAAGACCCCGUGAAGCUUUACUGUAGCUUAAUAUUGAUCAGGACAUUAUCAUGUAGAGAAUAGGUAGGAGCAAUCGAUGCAAGUUCGCUAGGACUUGUUGAUGCGAAAGGUGGAAUACUACCCUUGGUUGUGUGCUGUUCUAAUUGGUAACUGUUAUCCAGUUUCAAGACAGUGUUAGGUGGGCAGUUUGACUGGGGCGGUCGCCUCCUAAAAGGUAACGGAGGCGUACAAAGGUACCUUCAGUACGGUUGGAAAUCGUAUGUAGAGUGUAAUGGUGUAAGGGUGCUUGACUGUGAGACAUACAGGUCGAACAGGUGAGAAAUCAGGUCAUAGUGAUCCGGUGGUCCAGUAUGGAAUGGCCAUCGCUCAACGGAUAAAAGCUACUCCGGGGAUAACAGGCUGAUACUGCCCAAGAGUUCAUAUCGACGGCAGUGUUUGGCACCUCGAUGUCGACUCAUCUCAUCCUCGAGCUGAAGCAGGUUCGAAGGGUUCGGCUGUUCGCCGAUUAAAGAGAUACGUGAGUUGGGUUCAAACCGUCGUGAGACAGGUUGGUCCCUAUCUAUUGUGCCCGUAGGAAGAUUGAAGAGUGUUGCUUCUAGUACGAGAGGACCGAAGCGAGGACACCUCUUAUGCUCCAGUUGUAGCGCCAGCUGCACCGCUGGGUAGUAACGUGUCUAUUAGAUAAACGCUGAAAGCAUCUAAGUGUGAAACUAUCUCAAAGAUUAAUCUUCCCAUUUCGCAAGAAAGUAAGAGCCGUCAAAGACGAUGACGUUGAUAGGUUACAGGUGUAAGCAUAGUGAUAUGUUGAGCUGAGUAAUACUAAUUGCUCGAGGACUUAUUGGA

Entity 5 polymer

描述 description	5S ribosomal RNA
分子量 formula_weight	34796.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	4

UUUGGUGCCCAUGUCGCUGUGGAAACACCUGGUUCCAUUUCGAACCCAGCAGUUAAGCACAGUGGAGCCGAAUGUAGCUGUUUCAGUGAGAAUAGGAAAGCACCAAGC

Entity 6 polymer

描述 description	16S ribosomal RNA
分子量 formula_weight	491327.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	5

UUUUUCUGAGAGUUUGAUCCUGGCUCAGGAUUAACGCUGGCGGCAUGCCUAAUACAUGCAAGUCGAUCGAAAGUAGUAAUACUUUAGAGGCGAACGGGUGAGUAACACGUAUCCAAUCUACCUUAUAAUGGGGGAUAACUAGUUGAAAGACUAGCUAAUACCGCAUAAGAACUUUGGUUCGCAUGAAUCAAAGUUGAAAGGACCUGCAAGGGUUCGUUAUUUGAUGAGGGUGCGCCAUAUCAGCUAGUUGGUGGGGUAACGGCCUACCAAGGCAAUGACGUGUAGCUAUGCUGAGAAGUAGAAUAGCCACAAUGGGACUGAGACACGGCCCAUACUCCUACGGGAGGCAGCAGUAGGGAAUUUUUCACAAUGAGCGAAAGCUUGAUGGAGCAAUGCCGCGUGAACGAUGAAGGUCUUUAAGAUUGUAAAGUUCUUUUAUUUGGGAAGAAUGACUUUAGCAGGUAAUGGCUAGAGUUUGACUGUACCAUUUUGAAUAAGUGACGACUAACUAUGUGCCAGCAGUCGCGGUAAUACAUAGGUCGCAAGCGUUAUCCGGAUUUAUUGGGCGUAAAGCAAGCGCAGGCGGAUUGAAAAGUCUGGUGUUAAAGGCAGCUGCUUAACAGUUGUAUGCAUUGGAAACUAUUAAUCUAGAGUGUGGUAGGGAGUUUUGGAAUUUCAUGUGGAGCGGUGAAAUGCGUAGAUAUAUGAAGGAACACCAGUGGCGAAGGCGAAAACUUAGGCCAUUACUGACGCUUAGGCUUGAAAGUGUGGGGAGCAAAUAGGAUUAGAUACCCUAGUAGUCCACACCGUAAACGAUAGAUACUAGCUGUCGGGGCGAUCCCCUCGGUAGUGAAGUUAACACAUUAAGUAUCUCGCCUGGGUAGUACAUUCGCAAGAAUGAAACUCAAACGGAAUUGACGGGGACCCGCACAAGUGGUGGAGCAUGUUGCUUAAUUCGACGGUACACGAAAAACCUUACCUAGACUUGACAUCCUUGGCAAAAUUAUGGAAACAUAAUGGAGGUUAACCGAGUGACAGGUGGUGCAUGGUUGUCGUCAGCUCGUGUCGUGAGAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCGUUAGUUACAUUGUCUAGCGAGACUGCUAAUGCAAAUUGGAGGAAGGAAGGGAUGACGUCAAAUCAUCAUGCCCCUUAUGUCUAGGGCUGCAAACGUGCUACAAUGGCCAAUACAAACAGUCGCCAGCUUGUAAAAGUGAGCAAAUCUGUAAAGUUGGUCUCAGUUCGGAUUGAGGGCUGCAAUUCGUCCUCAUGAAGUCGGAAUCACUAGUAAUCGCGAAUCAGCUAUGUCGCGGUGAAUACGUUCUCGGGUCUUGUACACACCGCCCGUCAAACUAUGAAAGCUGGUAAUAUUUAAAAACGUGUUGCUAACCAUUAGGAAGCGCAUGUCAAGGAUAGCACCGGUGAUUGGAGUUAAGUCGUAACAAGGUACCCCUACGAGAACGUGGGGGUGGAUCACCUCCUUUCUA

Entity 7 polymer

描述 description	tRNA-Ala (E-site)
分子量 formula_weight	24440.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	6

GGGGAUGUAGCUCAACUGAUAGAGCACCUGAUUUGCACUCAGGAGGUUGAGGGUUUGAGACCCUUCAUCUCCACCA

Entity 8 polymer

描述 description	tRNA-Asp (P-site)
分子量 formula_weight	24136.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	7

GGUUCCAUGGUGUAGUGGAUAACAUAUCUCCCUGUCACGGAGGGGUUGCGGGUUCGAUUCCCGUUGGAACCGCCA

Entity 9 polymer

描述 description	tRNA-Lys (A-site)
分子量 formula_weight	24401.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	8

GCAUCUUUAGCUCAGUUGGUAGAGCAAAUGACUCUUAAUCAUUGGGUCGGGGGUUCGAGCCCCUCAAGAUGCACCA

Entity 10 polymer

描述 description	Trigger factor
分子量 formula_weight	51427.0 kDa
EC 编号 ec_number	5.2.1.8
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	X

MKQYKLVNTTQKEKTLCLEIAIDTKLWKETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVEFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH

Entity 11 polymer

描述 description	mRNA
分子量 formula_weight	2894.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polyribonucleotide
链编号 strand_id	Y

CCGAUAAGG

Entity 12 polymer

描述 description	nascent peptide
分子量 formula_weight	3127.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	Z

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKAAD

Entity 13 polymer

描述 description	50S ribosomal protein L2
分子量 formula_weight	31966.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	a

MPIKKIISRSNSGIHHSTVIDYKKLLTTNKNKPEKSLLVTLKKHGGRNNQGKITVRHQGGRNKRKYRIIDFKRTHYDNIEATVKSIEYDPNRSCFVSLITYANGAKSYIISPDGIKVGDKILASEHPIDIKPGFSMPLAFIPEGTQVHNIELHPKGGGQIARSAGSYARILGQDETGKYVILQLLSGETRKFLKECRATVGVVSNLDHNLVVIGKAGRNRHRGIRPTVRGSAMNPNDHPHGGGEGRSPVGRDAPRTPWGKRHMGVKTRNMKKASTNLIIRNRKGEQY

Entity 14 polymer

描述 description	50S ribosomal protein L3
分子量 formula_weight	31298.2 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	b

MEIRGIFGVKVGMSQVFTTNNERLPITVIYCEPNQVAGVKTEAKDKYSATLLSFDTVENKKLNKPQQGFFEKNNLKPTKHLQEIRNMTGFEMGQQITPQNLFQVGEYVDVSAISKGRGFTGAIKRWNFKIGPLGHGAGYPHRFQGSVQAGRGGASAQRVFKGKKMSGHYGHEKVTVQNLRIVGFDEANMLVLVSGAIAGPEGGVVLIRTAKKKPGVVKPIELAVQTEKAPEAKPAKLSKKKQAKELAKAQAANQQTVEAKVDTPVVEPKPTEVKKAAPVVEKKGEDK

Entity 15 polymer

描述 description	50S ribosomal protein L4
分子量 formula_weight	23632.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	c

MAKLKLIKIDGSFETEPVKLSPGLIAKELKQQPVFDAVLVEQASWRQGTHSILTKGEVRGGGKKPYKQKHTGKARQGSTRNPHFVGGGIVFGPKPNRNYSLKLNKKAHTAALHTVWSEKLASDNTHLVDQNLFNKTEGKTKVMMQFLKSAKLLDKNVLFVVNTLNTNLEQSTSNIKNVQVKHLDKVSVRDLMLANALLVEKEVLKALEGKFK

Entity 16 polymer

描述 description	50S ribosomal protein L5
分子量 formula_weight	20270.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	d

MNNLKAHYQKTIAKELQKSFAFSSIMQVPRLEKIVINMGVGDAIRDSKFLESALNELHLISGQKPVATKAKNAISTYKLRAGQLIGCKVTLRGERMWAFLEKLIYVALPRVRDFRGLSLKSFDGRGNYTIGIKEQIIFPEIVYDDIKRIRGFDVTLVTSTNKDSEALALLRALNLPLVKG

Entity 17 polymer

描述 description	50S ribosomal protein L6
分子量 formula_weight	20624.1 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	e

MSKIGNRTITLDPAKVNLNFQKDHIAVKGPLGQIELKLPPNLPLKFELKDNNLQITRNNELKQSKIFHGTYNALITNAIIGVTQGFEKKLRLVGVGYRANVEGETLNLQLGYSHPIKEKIPKGLTVKVEKNTEITISGISKELVGQFATEVRKWRKPEPYKGKGVLYFDEVIVRKAGKTAEGKK

Entity 18 polymer

描述 description	50S ribosomal protein L9
分子量 formula_weight	17185.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	f

MKVILKQDVSNLGKRFDVVDVKDGYAIHFLFPKKLAAPLTKKSLQDRDLFLKKQQEHYEINKALSHKLKEVIEQTELHFSLKEHNGRPYGSIITKQIINQAHTKGMALQKFMFKDNVRLGFGDHEITLHIFEDTTAVLKVKVTPDNGVK

Entity 19 polymer

描述 description	50S ribosomal protein L10
分子量 formula_weight	17645.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	g

MEAKKDKAQQVADVSHLLSTSAGFVIFDYTSMSAIEATSIRKKLFKNGSKIKVIKNNILRRALKAGKFEGIDETAIKGKLAVAVGVNEIVETLKAVDGVVKAKEAMNFVCGYFDNRAFNSADLEKIAKLPGRNELYGMFLSVLQAPLRKFLYALEAVKAAK

Entity 20 polymer

描述 description	50S ribosomal protein L11
分子量 formula_weight	14803.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	h

MAKKTITRIAKINLLGGQAKPGPALASVGINMGEFTKQFNEKTKDKQGEMIPCVITAYNDKSFDFILKTTPVSILLKQAAKLEKGAKNAKTIVGKITMAKAKEIAQYKLVDLNANTVEAALKMVLGTAKQMGIEVIE

Entity 21 polymer

描述 description	50S ribosomal protein L13
分子量 formula_weight	16821.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	i

MQKTSMLTKEQANKRRQWYIVDAAGLVLGKLAVKAADLIRGKNKVDFTPNQDCGDYLIIINSDQVVLTGNKKENEFWYHHSQYIGGIKKVSGRDMLKKQSDKLVYNAVKGMLPDNRLSRRWITKVHVFKGDKHNMEAQKPTTLNWS

Entity 22 polymer

描述 description	50S ribosomal protein L14
分子量 formula_weight	13478.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	j

MVSFMTRLNVADNTGAKQVGIIKVLGSTRKRYAFLGDVVVVSVKDAIPSGMVKKGQVLRAVIVRTKKGQQRKDGTHLKFDDNACVLIKEDKSPRGTRIFGPVARELRERGYNKILSLAVEVV

Entity 23 polymer

描述 description	50S ribosomal protein L15
分子量 formula_weight	16768.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	k

MELNQLKSVPKARNHKTKTLGRGHGSGLGKTSGRGQKGQKARKSGLTRPGFEGGQTPLYRRLPKFGNARKGFLKQEWVVLNLNKIAKLKLDKINRASLIEKQVISAKSQLPIKLIGHTKLEKPLHFEVHKVSKQALKAVENANGSVKLLEK

Entity 24 polymer

描述 description	50S ribosomal protein L16
分子量 formula_weight	15646.5 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	l

MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVTWKILKREEVSA

Entity 25 polymer

描述 description	50S ribosomal protein L17
分子量 formula_weight	14270.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	m

MSYINKPGKTSAWRVMTVRQQVSAVLAYGKIETTLKKAKNTQKRLDKLITLAKVDNFNNRRQVKKWLLNTNLFDVDQLMDHLFSKVAPKYEKTPGGYSRVLKLGPRRGDATEMAILQLTDAKYK

Entity 26 polymer

描述 description	50S ribosomal protein L18
分子量 formula_weight	13073.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	n

MKTRTEQRRLRHKRIVKKIRATNHDNRVVLMVIKSLNHISVQAWDFSQNIVLASSSSLALKLKNGNKDNAKLVGQDIADKLVKLKLTNVVFDTGGSKYHGRIAALAEAARERGLNF

Entity 27 polymer

描述 description	50S ribosomal protein L19
分子量 formula_weight	13870.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	o

MKKINKQALIDLVEQKQLKAYVPEFSAGDEVNVAIKLKEKEKVRIQNFTGTVLRRRGKGISETFIVRKTTDGIPIEKNFQIHNPNISIELKRRGKVRRAYISYMRERSGKAAKIKERKQ

Entity 28 polymer

描述 description	50S ribosomal protein L20
分子量 formula_weight	14742.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	p

MRIKGGKQTRVRRKKWLKQASGSFGTRHASYKVAKQTVIQAAKYAYRDRRNKKRDFRSLWILRLNAALREQGMTYSVFINLLKKHNIEINRKVLSELAIKEPSKFNLIVQKVKSEQPKAAKPAALGN

Entity 29 polymer

描述 description	50S ribosomal protein L21
分子量 formula_weight	11664.8 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	q

MHAIVVCGSKQYLVHENDTFFVEKLEAPVGKEIQLDKVLMLDEKIGAPYLEKARVVCVVEKHGLQRKVNVIKHISQKHHLKKYGHRQPYTKLKVVRFVHD

Entity 30 polymer

描述 description	50S ribosomal protein L22
分子量 formula_weight	17355.4 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	r

MIAFAKQFRVRISPQKARLVCQLIVGKKTADAQNILSNTPKKAATLIAKLLNSAIANATNNHGMNGDALYVFECVANQGPSMKRTIPRAKGSSNMITKRSSNLVVKLSDNPNERQELIKQQKALVKKRVEGQQKAKMARQKAVTSVVKAPSKTQGGVQK

Entity 31 polymer

描述 description	50S ribosomal protein L23
分子量 formula_weight	25892.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	s

MDVTNVLLKPVLTEKVYFNQMGETKKYVFVVNPKASKTRVKLAFELVYGIKPLKVNTLIRKPTTIRGGSRFPGLSKLEKLAVITLPKGIAISVTGEAPEKTDKPADKTTLKESTVKEIKDTKNSPEAVVKTAVEALQIKPTAAPVTTAPLQTVAVKVAKEVKEVKVEKPVKVEKPTKPAKVAKEAKTTKVAKETKAEKSVQTTKVAKETKTEKSAKTTKTTATKTTKTKTTKKEVKK

Entity 32 polymer

描述 description	50S ribosomal protein L24
分子量 formula_weight	12461.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	t

MQRIKKGDKVVVITGKNKGGSGIVLKIMPARQQAIVEGLNKVTRHKKKDQTTKRAAKQSTGKVQQEAPIFLSKLALFDQKAKQQTIGKIKYVMDPKTNKKTRVFKKSNNTL

Entity 33 polymer

描述 description	50S ribosomal protein L27
分子量 formula_weight	11527.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	u

MNNKYFLTKIDLQFFASKKGVGSTKNGRDSHAKRLGAKKADGQMIRTGQIIYRQRGTRVYPGVNVGLGSDDTLFALSDGLVKYQKFGPKQGKTRVSVVKHKLDA

Entity 34 polymer

描述 description	50S ribosomal protein L28
分子量 formula_weight	7549.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	v

MAKKDQLTLRGPLYGNNRSHSKTITRRKWNVNLQPCKVKTADGKTTRILVSTRTLRTLKKHNRLS

Entity 35 polymer

描述 description	50S ribosomal protein L29
分子量 formula_weight	13003.3 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	w

MTVAKELRQKSSEELVKLVIKLKGELLEYRFKLAHGELDKPHLINQTRRLLATILTILTERKLNWQEEQAKYKLLTKKTNEAAVNAWKQHLEANKAKLLKSRAKREDASKK

Entity 36 polymer

描述 description	50S ribosomal protein L31
分子量 formula_weight	10980.7 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	x

MKKDFHFPSQSVSFKCASCSNSFTIESTLKQKEITIDICGKCHPFYIGELTKQTVHGRAEKLSGKFNAGKAFLENKTPKKAKGKTEEYTKHRSLNEL

Entity 37 polymer

描述 description	50S ribosomal protein L32
分子量 formula_weight	6641.9 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	y

MAVQQRRSSKHRRDKRRSHDALTAQALSVCQKCGKKKLFHRVCSCGMYGDLRVKKAY

Entity 38 polymer

描述 description	50S ribosomal protein L33 1
分子量 formula_weight	6298.6 kDa
来源方法 src_method	nat
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	z

MAVKRSTRLGCNDCREINYLTFKNVKKNPEKLALNKFCSRCRKVVVHKEVKRK

Entity 39 non-polymer

描述 description	ZINC ION
分子量 formula_weight	65.4 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 40 non-polymer

描述 description	CHLORAMPHENICOL
分子量 formula_weight	323.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 41 non-polymer

描述 description	POTASSIUM ION
分子量 formula_weight	39.1 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

Entity 42 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	229

Entity 43 non-polymer

描述 description	1,4-DIAMINOBUTANE
分子量 formula_weight	88.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	7

Entity 44 non-polymer

描述 description	SPERMINE
分子量 formula_weight	202.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	4

Entity 45 non-polymer

描述 description	SPERMIDINE
分子量 formula_weight	145.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	16

Entity 46 non-polymer

描述 description	PENTANE-1,5-DIAMINE
分子量 formula_weight	102.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	3

Entity 47 non-polymer

描述 description	LYSINE
分子量 formula_weight	147.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	1

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.90 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	30774

fit	/app/data/saxs_computation/p8/8p8b/8p8b.fit
pddf	/app/data/pr_computation/p8/8p8b/8p8b.pr.out
profile	/app/data/saxs_computation/p8/8p8b/8p8b.dat

Mycoplasma pneumoniae large ribosomal subunit in chloramphenicol-treated cells

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

冷冻电镜 EM 3D

5. 实体与聚合体信息 (47)

Entity 1 polymer

聚合体 Polymer

Entity 2 polymer

聚合体 Polymer

Entity 3 polymer

聚合体 Polymer

Entity 4 polymer

聚合体 Polymer

Entity 5 polymer

聚合体 Polymer

Entity 6 polymer

聚合体 Polymer

Entity 7 polymer

聚合体 Polymer

Entity 8 polymer

聚合体 Polymer

Entity 9 polymer

聚合体 Polymer

Entity 10 polymer

聚合体 Polymer

Entity 11 polymer

聚合体 Polymer

Entity 12 polymer

聚合体 Polymer

Entity 13 polymer

聚合体 Polymer

Entity 14 polymer

聚合体 Polymer

Entity 15 polymer

聚合体 Polymer

Entity 16 polymer

聚合体 Polymer

Entity 17 polymer

聚合体 Polymer

Entity 18 polymer

聚合体 Polymer

Entity 19 polymer

聚合体 Polymer

Entity 20 polymer

聚合体 Polymer

Entity 21 polymer

聚合体 Polymer

Entity 22 polymer

聚合体 Polymer

Entity 23 polymer

聚合体 Polymer

Entity 24 polymer

聚合体 Polymer

Entity 25 polymer

聚合体 Polymer

Entity 26 polymer

聚合体 Polymer

Entity 27 polymer

聚合体 Polymer

Entity 28 polymer

聚合体 Polymer

Entity 29 polymer

聚合体 Polymer

Entity 30 polymer

聚合体 Polymer

Entity 31 polymer

聚合体 Polymer

Entity 32 polymer

聚合体 Polymer

Entity 33 polymer

聚合体 Polymer

Entity 34 polymer

聚合体 Polymer

Entity 35 polymer