Gamma-aminobutyric acid receptor subunit beta-3
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Other combination Heteromer Protein × 10 其他Polymer 10 PDB declaration: decameric（10） Consistent with protein copy count | Chain A； UniProt 26–332 | Not recorded | Megabody Mb25 × 5 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose × 5 ;alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose ; × 5 R16 HEXADECANE × 5 D10 DECANE × 20 HSM HISTAMINE × 5 CL CHLORIDE ION × 10 V8D Etomidate × 5 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4 cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 3.60 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 8PVB | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 4COF Crystal structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer Deposited 2014-01-28 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain A
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain B
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain B
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain C
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain C
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain D
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain D
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
Chain E
26–312（287 aa）
Fragment：RESIDUES 26-312,447-473
Chain E
447–473（27 aa）
Fragment：RESIDUES 26-312,447-473
|
Not recorded | BEN BENZAMIDINE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 CL CHLORIDE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；11.5% PEG4000 100 MM SODIUM CHLORIDE, 100 MM LITHIUM SULPHATE, 100 MM N-2-ACETAMIDO-IMINODIACETIC ACID, PH 6.5, 2% (W/V) BENZAMIDINE
|
Resolution 2.97 Å R-free 0.226 |
| 5O8F Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 and pregnanolone Deposited 2017-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–246（221 aa）
Chain B
26–246（221 aa）
Chain C
26–246（221 aa）
Chain D
26–246（221 aa）
Chain E
26–246（221 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 P9N Pregnanolone × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；14% PEG 6000, 0.1M N-(2-Acetamido)iminodiacetic acid (ADA), 7.6 mM 4-Cyclohexyl-1-Butyl-beta-D-Maltoside (CYMAL-4)
|
Resolution 3.20 Å R-free 0.242 |
| 5OJM Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25 Deposited 2017-07-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–242（217 aa）
Chain B
26–242（217 aa）
Chain C
26–242（217 aa）
Chain D
26–242（217 aa）
Chain E
26–242（217 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.8；277.15 K；14% poly-ethylene glycol (PEG) 5000, 0.08M magnesium acetate, 0.1M sodium citrate, 1mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)
|
Resolution 3.30 Å R-free 0.250 |
| 6A96 Cryo-EM structure of the human alpha5beta3 GABAA receptor in complex with GABA and Nb25 Deposited 2018-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain D
1–332（332 aa）
Chain D
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.51 Å |
| 6HUG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6HUJ CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 6HUK CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | H0Z bicuculline methochloride × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 6HUO CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with alprazolam (Xanax), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 08H 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 6HUP CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 6I53 Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc Deposited 2018-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6QFA CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25 Deposited 2019-01-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T Mutation：K279T | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.49 Å |
| 7A5V CryoEM structure of a human gamma-aminobutyric acid receptor, the GABA(A)R-beta3 homopentamer, in complex with histamine and megabody Mb25 in lipid nanodisc Deposited 2020-08-22 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: pentamer |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
|
Not recorded | R16 HEXADECANE × 5 D10 DECANE × 20 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 OCT N-OCTANE × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 10 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 1.70 Å |
| 7PBD a1b3 GABA-A receptor + GABA Deposited 2021-08-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
33–332（300 aa）
Chain B
447–473（27 aa）
Chain C
33–332（300 aa）
Chain C
447–473（27 aa）
Chain E
33–332（300 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 HSM HISTAMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 7PBZ a1b3 GABA-A receptor + GABA + Zn2+ Deposited 2021-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 UND UNDECANE × 2 OCT N-OCTANE × 2 HEX HEXANE × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZN ZINC ION × 1 HSM HISTAMINE × 1 D10 DECANE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.79 Å |
| 7PC0 GABA-A receptor bound by a-Cobratoxin Deposited 2021-08-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
33–332（300 aa）
Chain B
447–473（27 aa）
Chain C
33–332（300 aa）
Chain C
447–473（27 aa）
Chain E
33–332（300 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 HSM HISTAMINE × 1 ZN ZINC ION × 1 HEX HEXANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QN5 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 1 D10 DECANE × 2 OCT N-OCTANE × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 CL CHLORIDE ION × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 7QN6 Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QN7 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with GABA, histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | PX6 1,2-DIPALMITOYL-SN-GLYCERO-3-PHOSPHATE × 1 D10 DECANE × 4 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 ABU GAMMA-AMINO-BUTANOIC ACID × 1 R16 HEXADECANE × 2 HSM HISTAMINE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QN8 Cryo-EM structure of human full-length beta3delta GABA(A)R in complex with histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | HSM HISTAMINE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7QN9 Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with GABA, histamine and nanobody Nb25 in a pre-open/closed state Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 HSM HISTAMINE × 3 R16 HEXADECANE × 1 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QNA Cryo-EM structure of human full-length alpha4beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain D
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7QNB Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain D
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 7QNC Cryo-EM structure of human full-length extrasynaptic alpha4beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 D10 DECANE × 3 EI7 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one × 2 HSM HISTAMINE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 7QND Cryo-EM structure of human full-length extrasynaptic beta3delta GABA(A)R in complex with THIP (gaboxadol), histamine and nanobody Nb25 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain A
1–473（473 aa）
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain D
1–473（473 aa）
|
Not recorded | HSM HISTAMINE × 3 EI7 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 7QNE Cryo-EM structure of human full-length synaptic alpha1beta3gamma2 GABA(A)R in complex with Ro15-4513 and megabody Mb38 Deposited 2021-12-20 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 OCT N-OCTANE × 1 D10 DECANE × 4 CL CHLORIDE ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 EIE ethyl 8-[(azanylidene-$l^{4}-azanylidene)amino]-5-methyl-6-oxidanylidene-4~{H}-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8PET Cryo-EM structure of the full-length human alpha1beta3 GABA(A) receptor (babba arrangement) in complex with nanobody Nb25, expressed with a 5-fold excess of beta3 over alpha1 Deposited 2023-06-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–473（448 aa）
Chain D
26–473（448 aa）
Chain E
26–473（448 aa）
|
Not recorded | R16 HEXADECANE × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 12 ZN ZINC ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.34 Å |
| 9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
26–473（448 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.74 Å |
| 9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
Chain D
26–473（448 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
1–473（473 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain A
26–473（448 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9EQG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin Deposited 2024-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 D10 DECANE × 11 HEX HEXANE × 10 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 2 R16 HEXADECANE × 5 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 A1H6W Puerarin × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 100 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAS CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with pregnenolone sulfate Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | HEX HEXANE × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 17 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 R16 HEXADECANE × 5 A8W Pregnenolone sulfate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
32–472（441 aa）
Chain E
32–472（441 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FAU CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | HEX HEXANE × 6 D10 DECANE × 6 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 3 PLM PALMITIC ACID × 2 CLR CHOLESTEROL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FAV CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
Chain E
34–472（439 aa）
|
Not recorded | HEX HEXANE × 5 D10 DECANE × 4 R16 HEXADECANE × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FAW CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
30–472（443 aa）
Chain B
30–472（443 aa）
Chain D
30–472（443 aa）
Chain E
30–472（443 aa）
|
Not recorded | HEX HEXANE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAX CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain A
34–472（439 aa）
Chain B
34–472（439 aa）
Chain D
34–472（439 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R16 HEXADECANE × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 CLR CHOLESTEROL × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 HEX HEXANE × 13 D10 DECANE × 5 PLM PALMITIC ACID × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FEU Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 D10 DECANE × 15 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FEV Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FEW Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state (C1) Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FEX Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived symmetric bound-closed state (C5) Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FEY Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived symmetric bound-closed state (C1) Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FEZ Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric bound-closed state Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FF0 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric open state Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
29–332（304 aa）
Chain A
447–473（27 aa）
Chain B
29–332（304 aa）
Chain B
447–473（27 aa）
Chain C
29–332（304 aa）
Chain C
447–473（27 aa）
Chain D
29–332（304 aa）
Chain D
447–473（27 aa）
Chain E
29–332（304 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FF1 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the short-lived asymmetric desensitised state Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FF2 Cryo-EM structure of the beta3 homomeric GABA(A) receptor in complex with HSM in the long-lived symmetric desensitised state (C1) Deposited 2024-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain A
447–473（27 aa）
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain D
26–332（307 aa）
Chain D
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 HSM HISTAMINE × 5 D10 DECANE × 15 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFL Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFM Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with Mb25 in the resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9FFN Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 1 state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 3 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFO Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed 2 state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FFP Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FFQ Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 1 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFR Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric bound-closed state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | CLR CHOLESTEROL × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFS Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric open state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FFT Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the short-lived asymmetric desensitised state of branch 2 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFU Cryo-EM structure of the alpha1beta3 GABA(A) receptor in complex with GABA and Mb25 in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain C
26–332（307 aa）
Chain C
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 6 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FFV Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with Nb38 in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 5 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFW Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FFX Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FFY Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFZ Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FG0 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
33–332（300 aa）
Chain B
447–472（26 aa）
Chain E
33–332（300 aa）
Chain E
447–472（26 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FG1 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG2 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9FG3 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 bound twice in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain B
26–332（307 aa）
Chain B
447–473（27 aa）
Chain E
26–332（307 aa）
Chain E
447–473（27 aa）
|
Not recorded | D10 DECANE × 5 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG4 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FG5 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FG6 Cryo-EM structure of the full-length alpha1beta3 GABA(A) receptor in complex with GABA and HSM in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain C
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 HSM HISTAMINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FG7 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 3 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FG8 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FG9 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 R16 HEXADECANE × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FGA Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to two PIP2 molecules and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGB Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9FGC Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | D10 DECANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FGD Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs without PIP2 and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGF Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R16 HEXADECANE × 2 D10 DECANE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FGG Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FGH Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in large MSP2N2 nanodisc in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–473（473 aa）
Chain E
1–473（473 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9G2D Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived resting state (C5) sprayed with PBS Deposited 2024-07-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain B
26–332（307 aa）
Chain C
26–332（307 aa）
Chain D
26–332（307 aa）
Chain E
26–332（307 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9G2E Cryo-EM structure of the beta3 homomeric GABA(A) receptor in the long-lived desensitised state (C5) sprayed with PBS Deposited 2024-07-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Homooligomer；Protein × 5 PDB declaration: pentameric |
Chain A
26–332（307 aa）
Chain B
26–332（307 aa）
Chain C
26–332（307 aa）
Chain D
26–332（307 aa）
Chain E
26–332（307 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 D10 DECANE × 10 CL CHLORIDE ION × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9HAA a5b3 GABAA Receptor resting state Deposited 2024-11-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | BUA butanoic acid × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 ZN ZINC ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES, 100 mM NaCl, 2 mM Fluorinated Fos-choline 8, 0.005% LMNG, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.14 Å |
| 9HNQ a5b3 GABAA Receptor bound to GABA and Mb25 in desensitized state in detergent micelles Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM HEPES, 100 mM NaCl, 0.005% LMNG, 0.0005% CHS
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.81 Å |
| 9HNR a5b3 GABAA Receptor in 1 a5 to 4 b3 stoichiometry in desensitized state Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 8 PDB declaration: octameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain D
1–332（332 aa）
Chain D
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.17 Å |
| 9HNS a5b3 GABAAR bound to GABA and Mb25 in a desensitized state in saposin nanodiscs after long GABA treatment Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9HNT a5b3 GABAAR bound to Etomidate, GABA, and Mb25 in a desensitized state in saposin nanodiscs Deposited 2024-12-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | V8D Etomidate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.32 Å |
| 9HUM a5b3 GABAAR bound to GABA and Mb25 in a desensitized state in saposin nanodiscs after short GABA treatment Deposited 2024-12-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain C
1–332（332 aa）
Chain E
1–332（332 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.35 Å |
| 9RL5 a5b3 GABAAR bound to Topiramate, GABA, and Mb25 in a desensitized state in saposin nanodiscs Deposited 2025-06-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 TOR [(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9RPB a5b3 GABAAR bound to GABA, and Mb25 in a desensitized state in saposin nanodiscs, topiramate-free Deposited 2025-06-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain B
1–332（332 aa）
Chain B
447–473（27 aa）
Chain C
1–332（332 aa）
Chain C
447–473（27 aa）
Chain E
1–332（332 aa）
Chain E
447–473（27 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
94 other PDB entries and 94 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | GBRB3_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 56–362； UniProt 26–332 |
