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8rt7

Conformation-B of the full-length outer membrane core complex (TrwH/VirB7, TrwF/VirB9, TrwE/VirB10CTD) from the fully-assembled R388 type IV secretion system determined by cryo-EM.

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

TrwE protein

Escherichia coli

UniProt A8R758

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 46 TrwF protein × 16 （A8R757） TrwH × 14 （A7KZV0） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
8rt7 8rtd

2 assemblies

View Construct and Data Evidence
UniProt name A8R758_SALDU
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–395； UniProt 1–395 Author chain D； PDBConstruct 1–395； UniProt 1–395 Author chain G； PDBConstruct 1–395； UniProt 1–395 Author chain J； PDBConstruct 1–395； UniProt 1–395 Author chain M； PDBConstruct 1–395； UniProt 1–395 Author chain P； PDBConstruct 1–395； UniProt 1–395 Author chain S； PDBConstruct 1–395； UniProt 1–395 Author chain V； PDBConstruct 1–395； UniProt 1–395 Author chain Y； PDBConstruct 1–395； UniProt 1–395 Author chain b； PDBConstruct 1–395； UniProt 1–395 Author chain e； PDBConstruct 1–395； UniProt 1–395 Author chain h； PDBConstruct 1–395； UniProt 1–395 Author chain k； PDBConstruct 1–395； UniProt 1–395 Author chain n； PDBConstruct 1–395； UniProt 1–395 Author chain u； PDBConstruct 1–395； UniProt 1–395 Author chain y； PDBConstruct 1–395； UniProt 1–395

TrwF protein

Escherichia coli

UniProt A8R757

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 46 TrwE protein × 16 （A8R758） TrwH × 14 （A7KZV0） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
8rt7

1 assemblies

View Construct and Data Evidence
UniProt name A8R757_SALDU
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B； PDBConstruct 1–266； UniProt 1–266 Author chain E； PDBConstruct 1–266； UniProt 1–266 Author chain H； PDBConstruct 1–266； UniProt 1–266 Author chain K； PDBConstruct 1–266； UniProt 1–266 Author chain N； PDBConstruct 1–266； UniProt 1–266 Author chain Q； PDBConstruct 1–266； UniProt 1–266 Author chain T； PDBConstruct 1–266； UniProt 1–266 Author chain W； PDBConstruct 1–266； UniProt 1–266 Author chain Z； PDBConstruct 1–266； UniProt 1–266 Author chain c； PDBConstruct 1–266； UniProt 1–266 Author chain f； PDBConstruct 1–266； UniProt 1–266 Author chain i； PDBConstruct 1–266； UniProt 1–266 Author chain l； PDBConstruct 1–266； UniProt 1–266 Author chain o； PDBConstruct 1–266； UniProt 1–266 Author chain v； PDBConstruct 1–266； UniProt 1–266 Author chain z； PDBConstruct 1–266； UniProt 1–266

TrwH

Escherichia coli

UniProt A7KZV0

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 46 TrwE protein × 16 （A8R758） TrwF protein × 16 （A8R757） Consistent with protein count

Other States of the Same Protein in the Database

Monomer

None

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
8rt7

1 assemblies

View Construct and Data Evidence
UniProt name A7KZV0_SALDU
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C； PDBConstruct 1–47； UniProt 1–47 Author chain F； PDBConstruct 1–47； UniProt 1–47 Author chain I； PDBConstruct 1–47； UniProt 1–47 Author chain L； PDBConstruct 1–47； UniProt 1–47 Author chain O； PDBConstruct 1–47； UniProt 1–47 Author chain R； PDBConstruct 1–47； UniProt 1–47 Author chain U； PDBConstruct 1–47； UniProt 1–47 Author chain X； PDBConstruct 1–47； UniProt 1–47 Author chain a； PDBConstruct 1–47； UniProt 1–47 Author chain d； PDBConstruct 1–47； UniProt 1–47 Author chain g； PDBConstruct 1–47； UniProt 1–47 Author chain j； PDBConstruct 1–47； UniProt 1–47 Author chain m； PDBConstruct 1–47； UniProt 1–47 Author chain p； PDBConstruct 1–47； UniProt 1–47

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8rt7
Deposition date deposition_date2024-01-25
Structure title titleConformation-B of the full-length outer membrane core complex (TrwH/VirB7, TrwF/VirB9, TrwE/VirB10CTD) from the fully-assembled R388 type IV secretion system determined by cryo-EM.
Keywords keywords;type IV secretion system type 4 secretion system T4SS OMCC Conformation-B core complex outer membrane complex R388 plasmid conjugation bacterial secretion secretion secretion system protein complex VirB10 VirB9 VirB7 TrwE TrwF TrwH, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8rt7__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8rt7__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8rt7__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）69.66 Å
Rg (electron density)69.08 Å
Total Rg69.19 Å
Atom count55802
Residues7184
Excluded volume982500 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8rt7__assembly_1__model_1 46-meric （46） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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7. Citations (1)