8uaw

Cryo-EM Structure of Brucella Abortus Lumazine Synthase (BLS) Engineered with Shiga Toxin II subunit B (Stx2B)

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Shiga toxin II subunit B,6,7-dimethyl-8-ribityllumazine synthase 2

Brucella abortus bv. 1 str. 9-941

UniProt A7UQX3

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Homooligomer Protein 10 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A7UQX3_ECO57
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 3–72; UniProt 20–89 Author chain B; PDBConstruct 3–72; UniProt 20–89 Author chain C; PDBConstruct 3–72; UniProt 20–89 Author chain D; PDBConstruct 3–72; UniProt 20–89 Author chain E; PDBConstruct 3–72; UniProt 20–89 Author chain F; PDBConstruct 3–72; UniProt 20–89 Author chain G; PDBConstruct 3–72; UniProt 20–89 Author chain H; PDBConstruct 3–72; UniProt 20–89 Author chain I; PDBConstruct 3–72; UniProt 20–89 Author chain J; PDBConstruct 3–72; UniProt 20–89

Shiga toxin II subunit B,6,7-dimethyl-8-ribityllumazine synthase 2

Brucella abortus bv. 1 str. 9-941

UniProt P61711

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Homooligomer Protein 10 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RISB2_BRUAB
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 84–234; UniProt 8–158 Author chain B; PDBConstruct 84–234; UniProt 8–158 Author chain C; PDBConstruct 84–234; UniProt 8–158 Author chain D; PDBConstruct 84–234; UniProt 8–158 Author chain E; PDBConstruct 84–234; UniProt 8–158 Author chain F; PDBConstruct 84–234; UniProt 8–158 Author chain G; PDBConstruct 84–234; UniProt 8–158 Author chain H; PDBConstruct 84–234; UniProt 8–158 Author chain I; PDBConstruct 84–234; UniProt 8–158 Author chain J; PDBConstruct 84–234; UniProt 8–158

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8uaw
Deposition date deposition_date2023-09-22
Structure title titleCryo-EM Structure of Brucella Abortus Lumazine Synthase (BLS) Engineered with Shiga Toxin II subunit B (Stx2B)
Keywords keywordsCHIMERA, SHIGA, TOXIN, IMMUNOGEN; TOXIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8uaw__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8uaw__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8uaw__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)47.87 Å
Rg (electron density)47.66 Å
Total Rg47.97 Å
Atom count17830
Residues2320
Excluded volume315170 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8uaw__assembly_1__model_1 decameric (10) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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7. Citations (1)