9cf8

Cryo-EM structure of human kidney V-ATPase state 1

Method: ELECTRON MICROSCOPY

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

V-type proton ATPase 21 kDa proteolipid subunit

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATO_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 0； PDBConstruct 1–205； UniProt 1–205

V-type proton ATPase 16 kDa proteolipid subunit

OrganismNot specified

UniProt P27449

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATL_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain 1； PDBConstruct 1–155； UniProt 1–155 Author chain 2； PDBConstruct 1–155； UniProt 1–155 Author chain 3； PDBConstruct 1–155； UniProt 1–155 Author chain 4； PDBConstruct 1–155； UniProt 1–155 Author chain 5； PDBConstruct 1–155； UniProt 1–155 Author chain 6； PDBConstruct 1–155； UniProt 1–155 Author chain 7； PDBConstruct 1–155； UniProt 1–155 Author chain 8； PDBConstruct 1–155； UniProt 1–155 Author chain 9； PDBConstruct 1–155； UniProt 1–155

V-type proton ATPase catalytic subunit A

OrganismNot specified

UniProt P38606

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATA_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain A； PDBConstruct 1–617； UniProt 1–617 Author chain B； PDBConstruct 1–617； UniProt 1–617 Author chain C； PDBConstruct 1–617； UniProt 1–617

V-type proton ATPase subunit B, brain isoform

OrganismNot specified

UniProt P21281

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATB2_HUMAN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–511； UniProt 1–511 Author chain E； PDBConstruct 1–511； UniProt 1–511 Author chain F； PDBConstruct 1–511； UniProt 1–511

V-type proton ATPase subunit D

OrganismNot specified

UniProt Q9Y5K8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATD_HUMAN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain G； PDBConstruct 1–247； UniProt 1–247

V-type proton ATPase subunit E 1

OrganismNot specified

UniProt P36543

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATE1_HUMAN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain H； PDBConstruct 1–226； UniProt 1–226 Author chain I； PDBConstruct 1–226； UniProt 1–226 Author chain J； PDBConstruct 1–226； UniProt 1–226

V-type proton ATPase subunit G 1

OrganismNot specified

UniProt O75348

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATG1_HUMAN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain K； PDBConstruct 1–118； UniProt 1–118 Author chain L； PDBConstruct 1–118； UniProt 1–118 Author chain M； PDBConstruct 1–118； UniProt 1–118

V-type proton ATPase subunit F

OrganismNot specified

UniProt Q16864

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VATF_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain N； PDBConstruct 1–119； UniProt 1–119

V-type proton ATPase subunit d 1

OrganismNot specified

UniProt P61421

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VA0D1_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain Q； PDBConstruct 1–351； UniProt 1–351

V-type proton ATPase 116 kDa subunit a isoform 1

OrganismNot specified

UniProt Q93050

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VPP1_HUMAN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain R； PDBConstruct 1–837； UniProt 1–837

V-type proton ATPase subunit e 1

OrganismNot specified

UniProt O15342

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VA0E1_HUMAN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain S； PDBConstruct 1–81； UniProt 1–81

Ribonuclease kappa

OrganismNot specified

UniProt Q6P5S7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） V-type proton ATPase subunit S1 × 1 （Q15904） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RNK_HUMAN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain T； PDBConstruct 1–137； UniProt 1–137

V-type proton ATPase subunit S1

OrganismNot specified

UniProt Q15904

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） Renin receptor × 1 （O75787） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	VAS1_HUMAN
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain U； PDBConstruct 1–470； UniProt 1–470

Renin receptor

OrganismNot specified

UniProt O75787

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 30	V-type proton ATPase 21 kDa proteolipid subunit × 1 （Q99437） V-type proton ATPase 16 kDa proteolipid subunit × 9 （P27449） V-type proton ATPase catalytic subunit A × 3 （P38606） V-type proton ATPase subunit B, brain isoform × 3 （P21281） V-type proton ATPase subunit D × 1 （Q9Y5K8） V-type proton ATPase subunit E 1 × 3 （P36543） V-type proton ATPase subunit G 1 × 3 （O75348） V-type proton ATPase subunit F × 1 （Q16864） V-type proton ATPase subunit d 1 × 1 （P61421） V-type proton ATPase 116 kDa subunit a isoform 1 × 1 （Q93050） V-type proton ATPase subunit e 1 × 1 （O15342） Ribonuclease kappa × 1 （Q6P5S7） V-type proton ATPase subunit S1 × 1 （Q15904） ADENOSINE-5'-DIPHOSPHATE × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RENR_HUMAN
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain V； PDBConstruct 1–350； UniProt 1–350

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	9cf8
Deposition date deposition_date	2024-06-27
Structure title title	Cryo-EM structure of human kidney V-ATPase state 1
Keywords keywords	human, kidney, V-ATPase, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method method	ELECTRON MICROSCOPY

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9cf8__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9cf8__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9cf8__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	82.01 Å
Rg (electron density)	83.26 Å
Total Rg	83.03 Å
Atom count	56307
Residues	7287
Excluded volume	1013700 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9cf8__assembly_1__model_1`	30-meric （30）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (15)

Entity 1 Entity 1 polymer

Description description	V-type proton ATPase 21 kDa proteolipid subunit
Molecular weight formula_weight	21418.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	0
Atom count atom_count	56307

MTGLALLYSGVFVAFWACALAVGVCYTIFDLGFRFDVAWFLTETSPFMWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAIYGIIMAIVISNMAEPFSATDPKAIGHRNYHAGYSMFGAGLTVGLSNLFCGVCVGIVGSGAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD

Entity 2 Entity 2 polymer

Description description	V-type proton ATPase 16 kDa proteolipid subunit
Molecular weight formula_weight	15743.7 kDa
Source method src_method	nat
Copy count entity_count	9

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	1,2,3,4,5,6,7,8,9

MSESKSGPEYASFFAVMGASAAMVFSALGAAYGTAKSGTGIAAMSVMRPEQIMKSIIPVVMAGIIAIYGLVVAVLIANSLNDDISLYKSFLQLGAGLSVGLSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYGLIVALILSTK

Entity 3 Entity 3 polymer

Description description	V-type proton ATPase catalytic subunit A
Molecular weight formula_weight	68379.9 kDa
EC 编号 ec_number	7.1.2.2
Source method src_method	nat
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B,C

MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

Entity 4 Entity 4 polymer

Description description	V-type proton ATPase subunit B, brain isoform
Molecular weight formula_weight	56561.5 kDa
Source method src_method	nat
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D,E,F

MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH

Entity 5 Entity 5 polymer

Description description	V-type proton ATPase subunit D
Molecular weight formula_weight	28311.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MSGKDRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDALTLRFRQILKKIIETKMLMGEVMREAAFSLAEAKFTAGDFSTTVIQNVNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDSYELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKITNRRVNAIEHVIIPRIERTLAYIITELDEREREEFYRLKKIQEKKKILKEKSEKDLEQRRAAGEVLEPANLLAEEKDEDLLFE

Entity 6 Entity 6 polymer

Description description	V-type proton ATPase subunit E 1
Molecular weight formula_weight	26183.3 kDa
Source method src_method	nat
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	H,I,J

MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Entity 7 Entity 7 polymer

Description description	V-type proton ATPase subunit G 1
Molecular weight formula_weight	13781.5 kDa
Source method src_method	nat
Copy count entity_count	3

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K,L,M

MASQSQGIQQLLQAEKRAAEKVSEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKAKEAAALGSRGSCSTEVEKETQEKMTILQTYFRQNRDEVLDNLLAFVCDIRPEIHENYRING

Entity 8 Entity 8 polymer

Description description	V-type proton ATPase subunit F
Molecular weight formula_weight	13388.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR

Entity 9 Entity 9 polymer

Description description	V-type proton ATPase subunit d 1
Molecular weight formula_weight	40369.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MSFFPELYFNVDNGYLEGLVRGLKAGVLSQADYLNLVQCETLEDLKLHLQSTDYGNFLANEASPLTVSVIDDRLKEKMVVEFRHMRNHAYEPLASFLDFITYSYMIDNVILLITGTLHQRSIAELVPKCHPLGSFEQMEAVNIAQTPAELYNAILVDTPLAAFFQDCISEQDLDEMNIEIIRNTLYKAYLESFYKFCTLLGGTTADAMCPILEFEADRRAFIITINSFGTELSKEDRAKLFPHCGRLYPEGLAQLARADDYEQVKNVADYYPEYKLLFEGAGSNPGDKTLEDRFFEHEVKLNKLAFLNQFHFGVFYAFVKLKEQECRNIVWIAECIAQRHRAKIDNYIPIF

Entity 10 Entity 10 polymer

Description description	V-type proton ATPase 116 kDa subunit a isoform 1
Molecular weight formula_weight	96512.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWTEETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYSGQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDADEPSEDEVFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLFFFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEE

Entity 11 Entity 11 polymer

Description description	V-type proton ATPase subunit e 1
Molecular weight formula_weight	9380.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MAYHGLTVPLIVMSVFWGFVGFLVPWFIPKGPNRGVIITMLVTCSVCCYLFWLIAILAQLNPLFGPQLKNETIWYLKYHWP

Entity 12 Entity 12 polymer

Description description	Ribonuclease kappa
Molecular weight formula_weight	15435.2 kDa
EC 编号 ec_number	3.1.-.-
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MGWLRPGPRPLCPPARASWAFSHRFPSPLAPRRSPTPFFMASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYNLYEQVSYNCFIAAGLYLLLGGFSFCQVRLNKRKEYMVR

Entity 13 Entity 13 polymer

Description description	V-type proton ATPase subunit S1
Molecular weight formula_weight	52067.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MMAAMATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQLLQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIWMGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV

Entity 14 Entity 14 polymer

Description description	Renin receptor
Molecular weight formula_weight	39045.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

MAVFVVLLALVAGVLGNEFSILKSPGSVVFRNGNWPIPGERIPDVAALSMGFSVKEDLSWPGLAVGNLFHRPRATVMVMVKGVNKLALPPGSVISYPLENAVPFSLDSVANSIHSLFSEETPVVLQLAPSEERVYMVGKANSVFEDLSVTLRQLRNRLFQENSVLSSLPLNSLSRNNEVDLLFLSELQVLHDISSLLSRHKHLAKDHSPDLYSLELAGLDEIGKRYGEDSEQFRDASKILVDALQKFADDMYSLYGGNAVVELVTVKSFDTSLIRKTRTILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD

Entity 15 Entity 15 non-polymer

Description description	ADENOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	427.2 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.46 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	35623

Cryo-EM structure of human kidney V-ATPase state 1

1. Protein Identity and Related Structures Protein Identity & Related Structures

V-type proton ATPase 21 kDa proteolipid subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase 16 kDa proteolipid subunit

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase catalytic subunit A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit B, brain isoform

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit D

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit E 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit G 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit F

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase subunit d 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

V-type proton ATPase 116 kDa subunit a isoform 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer