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9h74

Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA

Method: ELECTRON MICROSCOPY Dmax: 243.2 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Eukaryotic translation initiation factor 2 subunit 1

OrganismNot specified

UniProt G1T2G4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain A； UniProt 1–284 Chain A； UniProt 166–271 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain P 6–271（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain A 1–284（284 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain A 6–271（266 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 6–271（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain A 1–284（284 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain A 1–284（284 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain A 1–284（284 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different construct Different mutation/modification Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain A 1–284（284 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1T2G4_RABIT
Isoform —
PDB entities 4
Chains and sequence ranges Author chain A； PDBConstruct 1–284； UniProt 1–284 Author chain A； PDBConstruct 285–390； UniProt 166–271

protein-synthesizing GTPase

OrganismNot specified

UniProt A0A5F9C3W2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain B； UniProt 1–500 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name A0A5F9C3W2_RABIT
Isoform —
PDB entities 5
Chains and sequence ranges Author chain B； PDBConstruct 1–485； UniProt 1–500

40S ribosomal protein SA

OrganismNot specified

UniProt G1TLT8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain C； UniProt 1–295 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain BB 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain C 1–209（209 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain C 2–209（208 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain C 2–209（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain B 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain B 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain B2 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain B 1–295（295 aa）
Mutation：T114A ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain B 1–295（295 aa）
Mutation：T114A ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SL 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SL 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain C 3–209（207 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain C 3–209（207 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AZ 1–295（295 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AB 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain AA 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain A 1–295（295 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain C 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AZ 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AZ 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AZ 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain C 1–295（295 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain q 1–295（295 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain B 1–295（295 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain q 1–222（222 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AZ 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AZ 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain L1 2–295（294 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain C 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain C 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain C 1–295（295 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AA 1–295（295 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

73 other PDB entries and 73 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TLT8_RABIT
Isoform —
PDB entities 6
Chains and sequence ranges Author chain C； PDBConstruct 1–295； UniProt 1–295

40S ribosomal protein S3a

OrganismNot specified

UniProt G1TN72

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain D； UniProt 1–264 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain B 1–264（264 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain B 1–264（264 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain B 1–264（264 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain i 19–233（215 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain D 1–264（264 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain D 1–264（264 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain D 19–233（215 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain D 19–233（215 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain D 19–233（215 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain x 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain D 1–264（264 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain D 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain D 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain D 1–264（264 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å

14 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TN72_RABIT
Isoform —
PDB entities 7
Chains and sequence ranges Author chain D； PDBConstruct 1–264； UniProt 1–264

Small ribosomal subunit protein uS5

OrganismNot specified

UniProt A0AAA9WW19

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain E； UniProt 1–270 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain E 1–270（270 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0AAA9WW19_RABIT
Isoform —
PDB entities 8
Chains and sequence ranges Author chain E； PDBConstruct 1–270； UniProt 1–270

Ribosomal protein S3

OrganismNot specified

UniProt G1TNM3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain F； UniProt 39–281 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–265（227 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–265（227 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–265（227 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain D 1–243（243 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain D 1–243（243 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain D 1–243（243 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain EE 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain EE 1–281（281 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain E1 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain E2 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain E1 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain E2 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain E 1–281（281 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain w 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain w 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain DD 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain w 39–266（228 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain E1 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain E 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain E 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain F 39–281（243 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain F 39–265（227 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 39–265（227 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Y3 39–265（227 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Y3 39–265（227 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain E 39–281（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain E 39–281（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain E2 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain DD 1–281（281 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain DD 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ac 1–281（281 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ac 1–281（281 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ac 1–281（281 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ac 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS03 39–266（228 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS03 39–266（228 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain DD 40–266（227 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain DD 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SO 1–281（281 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SO 1–281（281 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain E3 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ac 1–281（281 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain F 39–265（227 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain F 39–265（227 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ac 1–281（281 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain DD 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DB 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DB 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DB 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DB 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain DB 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain DB 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain DD 39–266（228 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS03 39–266（228 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS03 39–266（228 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS03 39–266（228 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain F 39–281（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ac 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ac 1–281（281 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ac 1–281（281 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain F 39–281（243 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain w 39–281（243 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain w 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain E 1–281（281 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain w 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ac 1–281（281 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ac 1–281（281 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain O1 39–281（243 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain F 1–243（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 1–243（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain F 1–243（243 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain DD 39–281（243 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

116 other PDB entries and 116 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TNM3_RABIT
Isoform —
PDB entities 9
Chains and sequence ranges Author chain F； PDBConstruct 1–243； UniProt 39–281

40S ribosomal protein S4

OrganismNot specified

UniProt G1TK17

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain G； UniProt 1–263 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain EE 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain EE 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain EE 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain E 1–263（263 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain E 1–263（263 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain E 1–263（263 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain I 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain EE 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain FF 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain F 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain x 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain x 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain x 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain x 2–263（262 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain F1 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain F 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain F 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain G 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain G 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain j3 2–263（262 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain j3 2–130（129 aa）
Chain j3 131–263（133 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain M 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain M 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain EE 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain EE 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain F 2–263（262 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain F3 2–263（262 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ad 1–263（263 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain G 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain G 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ad 1–263（263 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain EE 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain EE 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain EB 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain G 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ad 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ad 1–263（263 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ad 1–263（263 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain G 1–263（263 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain x 1–263（263 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain x 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain F 1–263（263 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ad 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ad 1–263（263 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain P1 1–263（263 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain G 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain G 1–263（263 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain EE 1–263（263 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

87 other PDB entries and 87 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TK17_RABIT
Isoform —
PDB entities 10
Chains and sequence ranges Author chain G； PDBConstruct 1–263； UniProt 1–263

Ribosomal protein S5

OrganismNot specified

UniProt G1TFM5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain H； UniProt 1–204 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain F 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain F 1–204（204 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain F 1–204（204 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain F 1–204（204 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain U 14–204（191 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain GG 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain GG 1–204（204 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BF 15–204（190 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain G1 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain G2 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain G1 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain G2 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain y 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain y 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain BB 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain y 14–204（191 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain G1 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain G 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain G 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 14–204（191 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain G 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain G2 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ae 1–204（204 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ae 1–204（204 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ae 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ae 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS05 14–204（191 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS05 14–204（191 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain FF 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain FF 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain G3 14–204（191 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ae 1–204（204 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ae 1–204（204 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8Q7Z Structure of the G. gallus 80S non-rotated ribosome Deposited 2023-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 80-meric
Chain Ae 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 494 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q87 Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1 Deposited 2023-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Ae 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 492 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain FB 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain FF 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS05 14–204（191 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS05 14–204（191 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS05 14–204（191 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain F 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ae 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ae 1–204（204 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ae 1–204（204 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain H 1–204（204 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain y 1–204（204 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain y 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain G 1–204（204 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain y 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ae 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ae 1–204（204 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Q1 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain H 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain H 1–204（204 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain FF 1–204（204 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

117 other PDB entries and 117 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TFM5_RABIT
Isoform —
PDB entities 11
Chains and sequence ranges Author chain H； PDBConstruct 1–204； UniProt 1–204

Small ribosomal subunit protein eS6

OrganismNot specified

UniProt G1TM55

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain I； UniProt 1–249 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain G 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain G 1–249（249 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain G 1–249（249 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain G 1–249（249 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain q 1–237（237 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain HH 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain HH 1–249（249 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BG 1–232（232 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain H1 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain H2 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain H1 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain H2 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain z 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain z 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain z 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain z 1–237（237 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain H1 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain H 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain H 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain I 1–237（237 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain I 1–237（237 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–237（237 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain b3 1–237（237 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain b3 1–237（237 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain H 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Af 1–249（249 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Af 1–249（249 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Af 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Af 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS06 1–237（237 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS06 1–237（237 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain H3 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Af 1–249（249 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Af 1–249（249 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain GG 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain GB 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain GG 1–237（237 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS06 1–237（237 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS06 1–237（237 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS06 1–237（237 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain G 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain I 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Af 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Af 1–249（249 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Af 1–249（249 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain I 1–249（249 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain z 1–249（249 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain z 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain H 1–249（249 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain z 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Af 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Af 1–249（249 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain R1 1–249（249 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain I 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain I 1–249（249 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain GG 1–249（249 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

117 other PDB entries and 117 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS6_RABIT
Isoform —
PDB entities 12
Chains and sequence ranges Author chain I； PDBConstruct 1–249； UniProt 1–249

40S ribosomal protein S7

OrganismNot specified

UniProt G1SVB0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain J； UniProt 1–194 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 5–193（189 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 5–193（189 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 5–193（189 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain H 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain H 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain H 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain H 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain X 5–194（190 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain II 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain II 1–194（194 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BH 12–194（183 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain I1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain I2 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain I1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain I2 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain BB 6–194（189 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain BB 6–194（189 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain y 6–194（189 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain BB 6–194（189 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain I1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain I 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain I 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain J 241–432（192 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain J 5–194（190 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 5–194（190 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain I 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain I2 5–194（190 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain HH 5–194（190 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ag 1–432（432 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ag 1–432（432 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ag 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ag 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain HH 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SS 1–432（432 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SS 1–432（432 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ag 1–432（432 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain J 5–194（190 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain J 5–194（190 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ag 1–432（432 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain HB 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain HB 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain HB 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain HB 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain HB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain HB 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain HH 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS07 6–194（189 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS07 6–194（189 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS07 6–194（189 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain H 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ag 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ag 1–432（432 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ag 1–432（432 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain J 1–194（194 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain BB 1–194（194 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain BB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain I 1–432（432 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain BB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ag 1–432（432 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ag 1–432（432 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain S1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain HH 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

116 other PDB entries and 116 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SVB0_RABIT
Isoform —
PDB entities 13
Chains and sequence ranges Author chain J； PDBConstruct 1–194； UniProt 1–194

Small ribosomal subunit protein eS8

OrganismNot specified

UniProt G1TJW1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain K； UniProt 1–208 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain I 1–208（208 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain I 1–208（208 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain I 1–208（208 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain o 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain JJ 1–208（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain JJ 2–207（206 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–207（207 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain CC 2–207（206 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain J1 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain J 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain J 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain K 2–207（206 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain K 2–207（206 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain c3 2–207（206 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain c3 2–207（206 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain J 1–208（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain J 1–208（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain J2 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ah 1–208（208 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ah 1–208（208 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ah 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ah 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS08 2–207（206 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS08 2–207（206 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain J3 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ah 1–208（208 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain K 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain K 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ah 1–208（208 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain II 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain IB 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain II 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS08 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS08 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS08 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain I 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain K 1–208（208 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ah 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ah 1–208（208 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ah 1–208（208 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain K 1–208（208 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain CC 1–208（208 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain J 1–208（208 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ah 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ah 1–208（208 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain T1 1–208（208 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 2–207（206 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain II 1–208（208 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

101 other PDB entries and 101 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS8_RABIT
Isoform —
PDB entities 14
Chains and sequence ranges Author chain K； PDBConstruct 1–208； UniProt 1–208

40S ribosomal protein S9

OrganismNot specified

UniProt B7NZS8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain L； UniProt 1–194 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain J 1–194（194 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain K 7–188（182 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain KK 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain KK 1–194（194 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BJ 2–180（179 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain K1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain K2 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain K1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain K2 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain DD 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain DD 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain XX 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain DD 2–186（185 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain K1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain K 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain K 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain L 7–188（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain L 7–188（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain L 7–188（182 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain d3 2–186（185 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain d3 2–186（185 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain K 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ai 1–194（194 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ai 1–194（194 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ai 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ai 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS09 2–186（185 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS09 2–186（185 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SU 1–194（194 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SU 1–194（194 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain K3 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ai 1–194（194 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain L 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain L 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ai 1–194（194 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8Q7Z Structure of the G. gallus 80S non-rotated ribosome Deposited 2023-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 80-meric
Chain Ai 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 494 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q87 Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1 Deposited 2023-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Ai 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 492 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain JJ 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain JB 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain JJ 2–186（185 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS09 2–186（185 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS09 2–186（185 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS09 2–186（185 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain J 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain L 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ai 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ai 1–194（194 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ai 1–194（194 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain L 1–194（194 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain DD 1–194（194 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain DD 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain K 1–194（194 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain DD 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ai 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ai 1–194（194 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain U1 1–194（194 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain L 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain L 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain L 1–194（194 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain JJ 1–194（194 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

123 other PDB entries and 123 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name B7NZS8_RABIT
Isoform —
PDB entities 15
Chains and sequence ranges Author chain L； PDBConstruct 1–194； UniProt 1–194

Small ribosomal subunit protein eS10

OrganismNot specified

UniProt G1U0Y5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain M； UniProt 1–149 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain LL 1–149（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain LL 1–149（149 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain L 1–149（149 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain L 1–149（149 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1U0Y5_RABIT
Isoform —
PDB entities 16
Chains and sequence ranges Author chain M； PDBConstruct 1–149； UniProt 1–149

40S ribosomal protein S11

OrganismNot specified

UniProt G1TRM4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain N； UniProt 1–158 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 2–153（152 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain L 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain L 1–158（158 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain L 1–158（158 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain L 1–158（158 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain G 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain MM 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain MM 1–158（158 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BL 6–158（153 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain M1 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain M2 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain M1 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain M2 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain M 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain EE 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain EE 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain EE 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain EE 3–153（151 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain M1 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain M 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain M 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain N 1–158（158 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain N 1–158（158 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain G3 2–154（153 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain G3 2–154（153 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain O 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain O 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain M2 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ak 1–158（158 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ak 1–158（158 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ak 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ak 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS11 3–153（151 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS11 3–153（151 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain LL 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain LL 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SW 1–158（158 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SW 1–158（158 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain M3 3–153（151 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ak 1–158（158 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain N 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain N 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ak 1–158（158 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain LB 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain LL 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS11 3–153（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS11 3–153（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS11 3–153（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain L 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain N 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ak 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ak 1–158（158 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ak 1–158（158 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain N 1–158（158 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain EE 1–158（158 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain EE 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain M 1–158（158 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain EE 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ak 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ak 1–158（158 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain W1 1–158（158 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain N 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain N 1–158（158 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain LL 1–158（158 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

123 other PDB entries and 123 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TRM4_RABIT
Isoform —
PDB entities 17
Chains and sequence ranges Author chain N； PDBConstruct 1–158； UniProt 1–158

40S ribosomal protein S12

OrganismNot specified

UniProt G1SFR8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain O； UniProt 1–132 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 9–132（124 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 9–132（124 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 9–132（124 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain M 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain M 1–132（132 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain M 1–132（132 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain M 1–132（132 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain r 9–132（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain NN 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain NN 1–132（132 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BM 11–130（120 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain N1 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain N2 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain N1 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain N2 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain MM 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain MM 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain MM 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain MM 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain RR 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain RR 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain RR 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain RR 14–130（117 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain N1 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain N 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain N 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain O 1–132（132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain O 1–132（132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 9–132（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain e3 9–132（124 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain e3 9–132（124 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain N 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain N2 10–132（123 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain MM 10–132（123 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain MM 9–132（124 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Al 1–132（132 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Al 1–132（132 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Al 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Al 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS12 14–130（117 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS12 14–130（117 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SX 1–132（132 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SX 1–132（132 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain N3 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Al 1–132（132 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain O 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain O 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Al 1–132（132 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain MM 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain MB 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain MM 14–130（117 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS12 14–130（117 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS12 14–130（117 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS12 14–130（117 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain M 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain O 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Al 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Al 1–132（132 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Al 1–132（132 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain O 1–132（132 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain RR 1–132（132 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain RR 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain N 1–132（132 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain RR 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Al 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Al 1–132（132 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain X1 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain O 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain O 1–132（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain MM 1–132（132 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SFR8_RABIT
Isoform —
PDB entities 18
Chains and sequence ranges Author chain O； PDBConstruct 1–132； UniProt 1–132

40S ribosomal protein S13

OrganismNot specified

UniProt G1SP51

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain P； UniProt 1–151 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain N 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain N 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain N 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain N 1–151（151 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain Z 2–151（150 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain OO 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain OO 1–151（151 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain O1 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain O2 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain O1 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain O2 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain O 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain QQ 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain QQ 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain QQ 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain QQ 2–150（149 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain O1 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain O 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain O 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain P 2–151（150 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain P 2–151（150 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain P 2–151（150 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain F3 2–151（150 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain F3 2–151（150 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain O2 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain NN 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Am 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Am 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Am 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Am 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS13 2–150（149 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS13 2–150（149 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain O3 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Am 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain P 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain P 2–151（150 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Am 1–151（151 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain NN 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain NB 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain NN 2–150（149 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS13 2–150（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS13 2–150（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS13 2–150（149 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain N 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Am 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Am 1–151（151 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Am 1–151（151 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain P 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain QQ 1–151（151 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain QQ 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain O 1–151（151 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain QQ 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Am 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Am 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Y1 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain P 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain NN 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SP51_RABIT
Isoform —
PDB entities 19
Chains and sequence ranges Author chain P； PDBConstruct 1–151； UniProt 1–151

40S ribosomal protein uS11

OrganismNot specified

UniProt G1T1F0

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain Q； UniProt 1–151 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 21–156（136 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 21–156（136 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 21–156（136 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain OO 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain PP 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain PP 1–151（151 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain MM 21–155（135 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain Q 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain Q 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 16–151（136 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain a5 16–151（136 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain a5 16–151（136 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain P2 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain OO 1–156（156 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain An 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain An 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain An 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain An 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain P3 16–151（136 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Q 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Q 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS14 16–150（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS14 16–150（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS14 16–150（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain O 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain 0 1–151（151 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain Q 1–151（151 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain MM 1–151（151 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain MM 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain MM 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain An 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain An 1–151（151 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Z1 1–151（151 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain OO 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain OO 1–151（151 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

42 other PDB entries and 42 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1T1F0_RABIT
Isoform —
PDB entities 20
Chains and sequence ranges Author chain Q； PDBConstruct 1–151； UniProt 1–151

40S ribosomal protein uS19

OrganismNot specified

UniProt G1U0Q2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain R； UniProt 1–145 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 4–130（127 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 4–130（127 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 4–130（127 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain P 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain P 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain P 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain n 4–130（127 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain QQ 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain QQ 1–145（145 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain Q1 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain Q2 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain Q1 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain Q2 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain PP 12–136（125 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain WW 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain WW 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 9 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain WW 12–131（120 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Q1 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain Q 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain Q 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain R 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain R 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 4–138（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain A3 4–130（127 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain A3 4–130（127 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain Q 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Q2 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ao 1–145（145 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ao 1–145（145 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ao 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ao 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS15 12–131（120 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS15 12–131（120 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sa 1–145（145 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sa 1–145（145 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain Q3 12–140（129 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ao 1–145（145 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain R 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain R 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ao 1–145（145 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain PB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain PP 12–131（120 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS15 12–131（120 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS15 12–131（120 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS15 12–131（120 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain R 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ao 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ao 1–145（145 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ao 1–145（145 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain R 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain WW 1–145（145 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain WW 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Q 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain WW 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ao 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ao 1–145（145 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain a1 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain R 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain R 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain PP 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1U0Q2_RABIT
Isoform —
PDB entities 21
Chains and sequence ranges Author chain R； PDBConstruct 1–145； UniProt 1–145

Ribosomal protein S16

OrganismNot specified

UniProt G1SGX4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain S； UniProt 27–172 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 32–172（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 32–172（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 32–172（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain Q 27–172（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain Q 27–172（146 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain Q 27–172（146 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Q 27–172（146 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain H 32–172（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain RR 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain RR 1–172（172 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain R1 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain R2 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain R1 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain R2 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain R 1–172（172 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain UU 31–172（142 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain UU 31–172（142 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain UU 31–172（142 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain UU 31–172（142 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain R1 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain R 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain R 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain S 32–172（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain S 32–172（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 32–172（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain B3 32–172（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain B3 32–172（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain R 27–172（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain R 27–172（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain R2 16–172（157 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ap 1–172（172 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ap 1–172（172 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ap 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ap 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sb 1–172（172 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sb 1–172（172 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain R3 31–172（142 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ap 1–172（172 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain S 32–172（141 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain S 32–172（141 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ap 1–172（172 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain QQ 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain QB 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain QB 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain QB 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain QB 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain QB 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain QB 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain QQ 31–172（142 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS16 31–172（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS16 31–172（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS16 31–172（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain Q 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain S 27–172（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ap 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ap 1–172（172 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ap 1–172（172 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain S 27–172（146 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain UU 27–172（146 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain UU 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain R 1–172（172 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain UU 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ap 1–172（172 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ap 1–172（172 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain b1 27–172（146 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain S 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain S 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain QQ 27–172（146 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

113 other PDB entries and 113 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SGX4_RABIT
Isoform —
PDB entities 22
Chains and sequence ranges Author chain S； PDBConstruct 1–146； UniProt 27–172

40S ribosomal protein eS17

OrganismNot specified

UniProt G1TU13

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain T； UniProt 1–135 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain R 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain R 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain R 1–135（135 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain e 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain SS 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain SS 1–135（135 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BR 2–126（125 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain S1 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain S2 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain S1 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain S2 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain KK 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain KK 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain YY 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain KK 2–133（132 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain S1 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain S 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain S 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain T 1–135（135 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain T 1–135（135 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain C3 1–129（129 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain C3 1–129（129 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain S 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain RR 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Aq 1–135（135 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Aq 1–135（135 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Aq 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Aq 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS17 2–133（132 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS17 2–133（132 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sc 1–135（135 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sc 1–135（135 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain S3 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Aq 1–135（135 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain T 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain T 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Aq 1–135（135 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain RR 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain RB 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain RR 2–133（132 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS17 2–133（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS17 2–133（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS17 2–133（132 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain R 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain T 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Aq 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Aq 1–135（135 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Aq 1–135（135 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain T 1–135（135 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain KK 1–135（135 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain KK 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain S 1–135（135 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain KK 2–135（134 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Aq 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Aq 1–135（135 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain c1 1–135（135 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain T 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain T 1–135（135 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain RR 1–135（135 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TU13_RABIT
Isoform —
PDB entities 23
Chains and sequence ranges Author chain T； PDBConstruct 1–135； UniProt 1–135

40S ribosomal protein uS13

OrganismNot specified

UniProt G1TPG3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain U； UniProt 1–152 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain S 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain S 1–152（152 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain S 1–152（152 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain S 1–152（152 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain L 6–142（137 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain TT 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain TT 1–152（152 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BS 4–142（139 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain T1 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain T2 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain T1 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain T2 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain T1 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain T 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain T 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain U 1–152（152 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain U 1–152（152 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 6–147（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain G5 6–142（137 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain G5 6–142（137 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain T 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain T2 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain SS 6–142（137 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS18 2–145（144 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS18 2–145（144 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain U 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain U 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ar 1–152（152 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8Q7Z Structure of the G. gallus 80S non-rotated ribosome Deposited 2023-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 80-meric
Chain Ar 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 494 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q87 Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1 Deposited 2023-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Ar 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 492 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain SS 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain SB 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain SS 2–145（144 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS18 2–145（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS18 2–145（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS18 2–145（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain S 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain U 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ar 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ar 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ar 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain U 1–152（152 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain II 1–152（152 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain II 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain T 1–152（152 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain II 1–152（152 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ar 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ar 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain d1 2–152（151 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain U 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain U 1–152（152 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain SS 1–152（152 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

116 other PDB entries and 116 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TPG3_RABIT
Isoform —
PDB entities 24
Chains and sequence ranges Author chain U； PDBConstruct 1–152； UniProt 1–152

40S ribosomal protein eS19

OrganismNot specified

UniProt G1TN62

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain V； UniProt 1–145 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 4–144（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 4–144（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 4–144（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain T 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain T 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain T 1–145（145 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain R 4–144（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain UU 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain UU 1–145（145 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain PP 3–143（141 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain PP 3–143（141 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain PP 3–143（141 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain PP 3–143（141 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain U1 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain U 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain U 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain V 4–144（141 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain V 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 4–144（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain H5 4–144（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain H5 4–144（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain U 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain U2 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain U3 3–143（141 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain V 4–144（141 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain V 4–144（141 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain TT 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain TB 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain TT 3–143（141 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS19 3–143（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS19 3–143（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS19 3–143（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain V 1–145（145 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain V 1–145（145 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain PP 1–145（145 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain U 1–145（145 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain PP 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain As 1–145（145 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain V 4–144（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 4–144（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain V 4–144（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain TT 1–145（145 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

100 other PDB entries and 100 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TN62_RABIT
Isoform —
PDB entities 25
Chains and sequence ranges Author chain V； PDBConstruct 1–145； UniProt 1–145

40S ribosomal protein uS10

OrganismNot specified

UniProt G1SIZ2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain W； UniProt 1–119 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 16–119（104 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 16–119（104 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 16–119（104 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain U 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain U 1–119（119 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain U 1–119（119 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain U 1–119（119 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain VV 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain VV 1–119（119 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain V1 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain V2 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain V1 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain V2 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain V1 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain V 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain V 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain W 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain W 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain V 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain At 1–119（119 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain At 1–119（119 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain At 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain At 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS20 18–117（100 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS20 18–117（100 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain UU 18–117（100 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain UU 18–117（100 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sf 1–119（119 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sf 1–119（119 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain At 1–119（119 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain W 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain W 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain At 1–119（119 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain UU 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain UB 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain UU 18–117（100 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS20 18–117（100 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS20 18–117（100 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS20 18–117（100 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain U 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain W 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain At 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain At 1–119（119 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain At 1–119（119 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain W 1–119（119 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain GG 1–119（119 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain GG 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain V 1–119（119 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain GG 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain At 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain At 1–119（119 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain f1 1–119（119 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain W 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain W 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain W 1–119（119 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain UU 1–119（119 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

107 other PDB entries and 107 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SIZ2_RABIT
Isoform —
PDB entities 26
Chains and sequence ranges Author chain W； PDBConstruct 1–119； UniProt 1–119

40S ribosomal protein S21

OrganismNot specified

UniProt Q32PB8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain X； UniProt 1–83 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain VV 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain VV 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain W2 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain VV 1–83（83 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain X 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain X 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain X 1–83（83 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS21_BOVIN
Isoform —
PDB entities 27
Chains and sequence ranges Author chain X； PDBConstruct 1–83； UniProt 1–83

Ribosomal protein S15a

OrganismNot specified

UniProt G1TG89

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain Y； UniProt 1–130 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain W 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain W 1–130（130 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain W 1–130（130 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain W 1–130（130 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain a 2–130（129 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain XX 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain XX 1–130（130 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain X1 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain X2 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain X1 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain X2 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain X 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain TT 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain TT 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain TT 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain TT 2–130（129 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain X1 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain X 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain X 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain Y 1–130（130 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain Y 1–130（130 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 2–130（129 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain J5 2–130（129 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain J5 2–130（129 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Av 1–130（130 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Av 1–130（130 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Av 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Av 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS22 2–130（129 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS22 2–130（129 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sh 1–130（130 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sh 1–130（130 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain X3 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Av 1–130（130 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Y 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Y 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Av 1–130（130 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain WW 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain WB 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain WW 2–130（129 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS22 2–130（129 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS22 2–130（129 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS22 2–130（129 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain W 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Av 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Av 1–130（130 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Av 1–130（130 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain Y 1–130（130 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain TT 1–130（130 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain TT 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain X 1–130（130 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain TT 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Av 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Av 1–130（130 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain h1 1–130（130 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain Y 1–130（130 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain WW 1–130（130 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TG89_RABIT
Isoform —
PDB entities 28
Chains and sequence ranges Author chain Y； PDBConstruct 1–130； UniProt 1–130

40S ribosomal protein S23

OrganismNot specified

UniProt G1SZ47

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain Z； UniProt 1–143 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain X 2–143（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain X 1–143（143 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain X 1–143（143 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain X 1–143（143 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain YY 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain YY 1–143（143 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain Y 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain VV 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain VV 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain VV 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain VV 2–142（141 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain Y 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain Y 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain Z 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain Z 1–142（142 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Z 1–142（142 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain T3 2–142（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain T3 2–142（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain c 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain c 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain XX 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Y2 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain XX 1–142（142 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain XX 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS23 2–142（141 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS23 2–142（141 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Si 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Si 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain Y3 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Z 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain Z 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain XX 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain XX 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain XB 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain XX 2–142（141 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS23 2–142（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS23 2–142（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS23 2–142（141 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain X 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain Z 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain Z 1–143（143 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain VV 1–143（143 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain VV 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain Y 1–143（143 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain VV 1–143（143 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Aw 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain i1 1–143（143 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Z 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Z 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain Z 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain XX 1–143（143 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

106 other PDB entries and 106 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SZ47_RABIT
Isoform —
PDB entities 29
Chains and sequence ranges Author chain Z； PDBConstruct 1–143； UniProt 1–143

40S ribosomal protein S24

OrganismNot specified

UniProt G1TS40

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain a； UniProt 1–133 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain Y 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain Y 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Y 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain s 3–133（131 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain a 1–133（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain a 1–133（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 3–128（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain a 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain a 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain a 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain a 1–133（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain a 3–128（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 3–128（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain a 3–128（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å

14 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TS40_RABIT
Isoform —
PDB entities 30
Chains and sequence ranges Author chain a； PDBConstruct 1–133； UniProt 1–133

40S ribosomal protein S26

OrganismNot specified

UniProt G1TFE8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain b； UniProt 1–115 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain a 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain a 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain a 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain k 2–100（99 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain bb 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain b 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain b 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain b 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain b 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 2–100（99 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain aa 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS26 2–102（101 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS26 2–102（101 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Aa 2–102（101 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Aa 2–102（101 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain b 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain b 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain aa 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain aa 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AC 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain aa 2–102（101 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS26 2–102（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS26 2–102（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS26 2–102（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain a 1–115（115 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain b 1–115（115 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain LL 1–115（115 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain b 2–102（101 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain b 1–115（115 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain aa 1–115（115 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

65 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TFE8_RABIT
Isoform —
PDB entities 31
Chains and sequence ranges Author chain b； PDBConstruct 1–115； UniProt 1–115

40S ribosomal protein S27

OrganismNot specified

UniProt G1TZ76

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain c； UniProt 1–84 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain b 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain b 1–84（84 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain b 1–84（84 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain b 1–84（84 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain Y 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain cc 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain cc 1–84（84 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain c1 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain c2 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain c1 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain c2 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain JJ 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain JJ 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain JJ 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain JJ 2–84（83 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain c1 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain c 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain c 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain c 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain c 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain L3 2–84（83 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain L3 2–84（83 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain e 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain e 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain c2 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AA 1–84（84 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AA 1–84（84 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AA 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AA 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain Bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS27 2–84（83 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS27 2–84（83 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain c3 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AA 1–84（84 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain c 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain c 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AA 1–84（84 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain bb 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain BC 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain bb 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS27 2–84（83 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS27 2–84（83 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS27 2–84（83 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain b 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AA 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AA 1–84（84 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AA 1–84（84 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain c 1–84（84 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain JJ 1–84（84 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain JJ 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain c 1–84（84 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain JJ 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AA 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AA 1–84（84 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain B1 1–84（84 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain c 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain bb 1–84（84 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

116 other PDB entries and 116 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TZ76_RABIT
Isoform —
PDB entities 32
Chains and sequence ranges Author chain c； PDBConstruct 1–84； UniProt 1–84

40S ribosomal protein S28

OrganismNot specified

UniProt G1TIB4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain d； UniProt 1–69 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 8–68（61 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 8–68（61 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 8–68（61 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain c 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain c 1–69（69 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain c 1–69（69 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain c 1–69（69 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain l 5–68（64 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain dd 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain dd 1–69（69 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Bc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain d1 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain d2 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain d1 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain d2 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain FF 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain FF 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain FF 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain FF 7–68（62 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain d1 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain d 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain d 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain d 1–69（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain d 1–69（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 5–68（64 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain f3 5–68（64 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain f3 5–68（64 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain d2 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AB 1–69（69 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AB 1–69（69 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AB 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AB 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain Cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS28 7–68（62 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS28 7–68（62 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain d3 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AB 1–69（69 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain d 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain d 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AB 1–69（69 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain cc 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain CC 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain cc 7–68（62 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS28 7–68（62 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS28 7–68（62 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS28 7–68（62 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain c 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain d 1–69（69 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AB 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AB 1–69（69 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AB 1–69（69 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain d 1–69（69 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain FF 1–69（69 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain FF 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain d 1–69（69 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain FF 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AB 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AB 1–69（69 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain C1 1–69（69 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain d 5–68（64 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 5–68（64 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain d 5–68（64 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain cc 1–69（69 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

122 other PDB entries and 122 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TIB4_RABIT
Isoform —
PDB entities 33
Chains and sequence ranges Author chain d； PDBConstruct 1–69； UniProt 1–69

40S ribosomal protein S29

OrganismNot specified

UniProt G1U7M4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain e； UniProt 1–56 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 4–56（53 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 4–56（53 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 4–56（53 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain d 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain d 1–56（56 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain d 1–56（56 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain d 1–56（56 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain J 4–56（53 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain ee 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Bd 5–55（51 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain e1 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain e2 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain e1 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain e2 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 9 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain 9 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain w 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain 9 2–56（55 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain e1 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain e 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain e 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain e 1–56（56 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain e 4–56（53 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 4–56（53 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain H3 4–56（53 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain H3 4–56（53 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain e2 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain dd 4–56（53 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AG 1–56（56 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AG 1–56（56 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AG 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AG 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain Dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS29 2–56（55 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS29 2–56（55 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SH 1–56（56 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SH 1–56（56 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain e3 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AG 1–56（56 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain e 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain e 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AG 1–56（56 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain dd 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain DC 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain dd 2–56（55 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS29 2–56（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS29 2–56（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS29 2–56（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain d 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AG 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AG 1–56（56 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AG 1–56（56 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain e 1–56（56 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain 9 1–56（56 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain 9 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain e 1–56（56 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain 9 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AG 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AG 1–56（56 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain H1 1–56（56 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain e 1–56（56 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain dd 1–56（56 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

120 other PDB entries and 120 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1U7M4_RABIT
Isoform —
PDB entities 34
Chains and sequence ranges Author chain e； PDBConstruct 1–56； UniProt 1–56

Ribosomal protein S27a

OrganismNot specified

UniProt G1SK22

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain f； UniProt 1–156 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 83–151（69 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 83–151（69 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 83–151（69 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain p 82–152（71 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain gg 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain gg 1–156（156 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Bf 79–151（73 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain g1 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain g2 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain g1 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain g2 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain ff 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain ff 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain ff 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ff 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 0 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain 0 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 0 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain 0 83–150（68 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain g1 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain f 114–184（71 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain f 82–152（71 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 82–152（71 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Q3 1–188（188 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain g 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain g2 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AC 1–156（156 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AC 1–156（156 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AC 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS31 83–150（68 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS31 83–150（68 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain g3 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AC 1–156（156 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain f 82–152（71 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain f 82–152（71 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AC 1–156（156 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ff 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FC 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FC 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FC 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain FC 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain FC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain FC 1–188（188 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ff 83–150（68 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain f 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AC 1–156（156 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AC 1–156（156 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain f 1–156（156 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain 0 1–156（156 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain 0 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain g 1–188（188 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain 0 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AC 1–156（156 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain D1 77–156（80 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain f 1–156（156 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ff 1–156（156 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

113 other PDB entries and 113 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SK22_RABIT
Isoform —
PDB entities 35
Chains and sequence ranges Author chain f； PDBConstruct 1–156； UniProt 1–156

Small ribosomal subunit protein RACK1

OrganismNot specified

UniProt G1SJB4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain g； UniProt 1–317 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain m 2–314（313 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain hh 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain hh 1–317（317 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Bg 2–315（314 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain h1 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain h2 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain h1 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain h2 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 6 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain 6 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain 6 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain 6 2–314（313 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain h1 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain h 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain h 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain g 2–314（313 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain g 2–314（313 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 2–314（313 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain a3 2–314（313 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain a3 2–314（313 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain h 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain h2 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AF 1–317（317 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AF 1–317（317 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AF 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AF 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain Gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain ARAC 2–314（313 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain ARAC 2–314（313 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain h3 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AF 1–317（317 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain g 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain g 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AF 1–317（317 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain gg 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain GC 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain gg 2–314（313 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ARAC 2–314（313 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain ARAC 2–314（313 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain ARAC 2–314（313 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain g 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AF 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AF 1–317（317 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AF 1–317（317 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain g 1–317（317 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain 6 1–317（317 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain 6 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain h 1–317（317 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain 6 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AF 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AF 1–317（317 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain G1 1–317（317 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain g 1–317（317 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain gg 1–317（317 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

121 other PDB entries and 121 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SJB4_RABIT
Isoform —
PDB entities 36
Chains and sequence ranges Author chain g； PDBConstruct 1–317； UniProt 1–317

40S ribosomal protein S30

OrganismNot specified

UniProt G1T8A2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain i； UniProt 1–133 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 77–133（57 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 77–133（57 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 77–133（57 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain e 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain e 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain e 1–133（133 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain V 75–133（59 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain ff 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain ff 1–133（133 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain f1 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain f2 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain f1 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain f2 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain AA 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain AA 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain AA 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AA 79–133（55 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain f1 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain f 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain f 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain i 1–133（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain i 75–133（59 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain i 75–133（59 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain f 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain f2 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AD 1–133（133 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AD 1–133（133 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain AD 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain AD 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain Ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS30 79–133（55 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS30 79–133（55 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain Ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain SE 1–133（133 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain SE 1–133（133 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain AD 1–133（133 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain i 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain i 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain AD 1–133（133 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ee 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain EC 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ee 79–133（55 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS30 79–133（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS30 79–133（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AS30 79–133（55 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain i 1–133（133 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain AD 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain AD 1–133（133 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain AD 1–133（133 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain i 1–133（133 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AA 1–133（133 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain AA 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain f 1–133（133 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain AA 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain AD 1–133（133 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain AD 1–133（133 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain E1 75–133（59 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain i 75–133（59 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain i 75–133（59 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain i 75–133（59 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ee 1–133（133 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

118 other PDB entries and 118 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1T8A2_RABIT
Isoform —
PDB entities 37
Chains and sequence ranges Author chain i； PDBConstruct 1–133； UniProt 1–133

Eukaryotic translation initiation factor 1A, X-chromosomal

OrganismNot specified

UniProt P47813

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain j； UniProt 1–144 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1D7Q HUMAN TRANSLATION INITIATION FACTOR EIF1A Deposited 1999-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–143（143 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 500mM；Pressure 1
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 500mM；Pressure 1
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 500mM；Pressure 1
NMR measurement conditions pH 7.5；298 K；Ionic strength (raw mmCIF value) 500mM；Pressure 1
NMR sample composition 0.8MM U-13C,15N EIF1A, 10MM PHOSPHATE BUFFER, PH 7.5, 500MM NACL, 1MM DTT, 0.1MM EDTA
NMR sample composition 0.5MM U-15N EIF1A, 10MM PHOSPHATE BUFFER, PH 7.5, 500MM NACL, 1MM DTT, 0.1MM EDTA
NMR sample composition 1.2MM EIF1A, 10MM PHOSPHATE BUFFER, PH 7.5, 500MM NACL, 1MM DTT, 0.1MM EDTA
NMR sample composition 0.4MM 15N-LYSINE EIF1A, 10MM PHOSPHATE BUFFER, PH 7.5, 500MM NACL, 1MM DTT, 0.1MM EDTA
Resolution not provided
28ZU human 48S PIC with Kozak mRNA and eIF1A W70A mutant Deposited 2026-03-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain H 1–144（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 3.20 Å
28ZV human 48S PIC with mRNA (non-Kozak) Deposited 2026-03-03 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain H 1–144（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 2.90 Å
28ZX human 48S PIC with mRNA (Kozak_1) Deposited 2026-03-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain H 1–144（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 2.80 Å
28ZY human 48S PIC with mRNA (Kozak_2) Deposited 2026-03-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain H 1–144（144 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY mmCIF provides none of the parsed conditions Resolution 3.00 Å
3ZJY Crystal Structure of Importin 13 - RanGTP - eIF1A complex Deposited 2013-01-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 1–112（112 aa） Fragment：RESIDUES 1-112
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.60 Å R-free 0.288
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain n 1–144（144 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain n 1–144（144 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
6YBW Structure of a human 48S translational initiation complex - 40S body Deposited 2020-03-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 24 PDB declaration: 26-meric
Chain q 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 60 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6ZMW Structure of a human 48S translational initiation complex Deposited 2020-07-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 57-meric
Chain q 1–144（144 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 89 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6ZP4 SARS-CoV-2 Nsp1 bound to a human 43S preinitiation ribosome complex - state 2 Deposited 2020-07-08 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric
Chain G 1–144（144 aa）
Not recorded ZN ZINC ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7A09 Structure of a human ABCE1-bound 43S pre-initiation complex - State III Deposited 2020-08-07 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 54-meric
Chain G 1–112（112 aa）
Not recorded ZN ZINC ION × 4 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 3 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MET METHIONINE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
7QP6 Structure of the human 48S initiation complex in open state (h48S AUG open) Deposited 2022-01-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain q 1–144（144 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Hepes, pH 7.5, 95 mM KOAc, 3.75 mM Mg(OAc)2, 1 mM ATP, 0.5 mM GTP, 0.25 mM spermidine, 2 mM DTT, 0.4 U/uL RiboLock RNase inhibitor
cryo-EM vitrification conditions Cryogen ETHANE；Cryo-EM grids were prepared by floating home-made continuous carbon on 40 ul sample in the wells of teflon block (custom-made). The sample-covered carbon was then adsorbed to an EM grid.
Resolution 4.70 Å
7QP7 Structure of the human 48S initiation complex in closed state (h48S AUG closed) Deposited 2022-01-03 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain q 1–144（144 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Hepes, pH 7.5, 95 mM KOAc, 3.75 mM Mg(OAc)2, 1 mM ATP, 0.5 mM GTP, 0.25 mM spermidine, 2 mM DTT, 0.4 U/uL RiboLock RNase inhibitor
cryo-EM vitrification conditions Cryogen ETHANE；Cryo-EM grids were prepared by floating home-made continuous carbon on 40 ul sample in the wells of teflon block (custom-made). The sample-covered carbon was then adsorbed to an EM grid.
Resolution 3.70 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
8OZ0 Structure of a human 48S translation initiation complex with eIF4F and eIF4A Deposited 2023-05-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 57-meric
Chain G 1–144（144 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 89 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
8PJ1 Structure of human 48S translation initiation complex in open codon scanning state (48S-1) Deposited 2023-06-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain q 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 90 ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 3.40 Å
8PJ2 Structure of human 48S translation initiation complex in AUG recognition state after eIF5-induced GTP hydrolysis by eIF2 (48S-2) Deposited 2023-06-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 49-meric
Chain q 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 87 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 3.40 Å
8PJ3 Structure of human 48S translation initiation complex upon transfer of initiator tRNA to eIF5B (48S-3) Deposited 2023-06-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain q 1–144（144 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 MG MAGNESIUM ION × 89 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 3.70 Å
8PJ4 Structure of human 48S translation initiation complex after eIF5 release (48S-4) Deposited 2023-06-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain q 1–144（144 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 NA SODIUM ION × 1 MG MAGNESIUM ION × 89 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 3.20 Å
8PJ5 Structure of human 48S translation initiation complex after eIF2 release prior 60S subunit joining (48S-5) Deposited 2023-06-22 Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain q 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 91 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 2.90 Å
8PJ6 Structure of human 48S translation initiation complex with initiator tRNA, eIF1A and eIF3 (off-pathway) Deposited 2023-06-22 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain q 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 87 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Manual blotting & plunge-freezing
Resolution 2.90 Å
8PPL MERS-CoV Nsp1 bound to the human 43S pre-initiation complex Deposited 2023-07-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 52-meric
Chain Iq 1–144（144 aa）
Not recorded ZN ZINC ION × 4 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 116 MET METHIONINE × 1 UNX UNKNOWN LIGAND × 129 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.65 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain A 1–144（144 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9KN5 Structure of the human 40S ribosome complexed with HCV IRES, eIF1A and eIF3 Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 46-meric
Chain 1A 1–144（144 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9KN6 Structure of the HCV IRES-dependent pre-48S translation initiation complex with eIF1A, eIF5B, and eIF3 Deposited 2024-11-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric
Chain 1A 1–144（144 aa）
Not recorded MG MAGNESIUM ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å

31 other PDB entries and 31 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name IF1AX_HUMAN
Isoform —
PDB entities 38
Chains and sequence ranges Author chain j； PDBConstruct 1–144； UniProt 1–144

ATP binding cassette subfamily E member 1

OrganismNot specified

UniProt A0A5K1V8K4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain k； UniProt 1–599 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name A0A5K1V8K4_PIG
Isoform —
PDB entities 39
Chains and sequence ranges Author chain k； PDBConstruct 1–599； UniProt 1–599

60S ribosomal protein L41

OrganismNot specified

UniProt A0A087WNH4

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain l； UniProt 1–25 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
6FTG Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes Deposited 2018-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 9UB [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；Blot 3 seconds before plunging.
Resolution 9.10 Å
6FTI Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome Deposited 2018-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 60-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 9UB [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
6FTJ Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome Deposited 2018-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 9UB [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.70 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain An 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain An 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain An 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain An 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain n2 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6T59 Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 and nascent chain-associated complex Deposited 2019-10-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain n3 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.11 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain l 1–25（25 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain l 1–25（25 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain l 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain X3 2–24（23 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain X3 2–24（23 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NFX Mammalian ribosome nascent chain complex with SRP and SRP receptor in early state A Deposited 2021-02-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 55-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 5 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.20 Å
7NWG Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain n3 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 299 BLS BLASTICIDIN S × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.80 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain n 2–24（23 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Az 1–25（25 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Az 1–25（25 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Az 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Az 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OBR RNC-SRP early complex Deposited 2021-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 97 ZN ZINC ION × 5 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7QWQ Ternary complex of ribosome nascent chain with SRP and NAC Deposited 2022-01-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 52-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 97 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.83 Å
7QWR Structure of the ribosome-nascent chain containing an ER signal sequence in complex with NAC Deposited 2022-01-25 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 48-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 5 MG MAGNESIUM ION × 97 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7QWS Structure of ribosome translating beta-tubulin in complex with TTC5 and NAC Deposited 2022-01-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 46 PDB declaration: 49-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 5 MG MAGNESIUM ION × 97 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.40 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYI Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYJ Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYK Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.20 Å
7SYL Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.50 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TM3 Structure of the rabbit 80S ribosome stalled on a 2-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-01-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 54-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50 mM HEPES-KOH, pH 7.5, 200 mM KOAc, 5 mM Mg(OAc)2, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE；blot for 4 sec with Whatman filter papers at a blot force of -15 before plunging
Resolution 3.25 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AL41 1–25（25 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7TUT Structure of the rabbit 80S ribosome stalled on a 4-TMD Rhodopsin intermediate in complex with the multipass translocon Deposited 2022-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 220 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；50 mM HEPES-KOH, pH 7.5, 200 mM potassium acetate, 5 mM magnesium acetate, 0.25% digitonin, 50 mM biotin
cryo-EM vitrification conditions Cryogen ETHANE；Blot for 4 seconds with Whatman filter papers at a blot force of -15 before plunging.
Resolution 3.88 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJW Rabbit 80S ribosome as it decodes the Sec-UGA codon Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Sl 1–25（25 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 2 SER SERINE × 1 MG MAGNESIUM ION × 225 K POTASSIUM ION × 18 ZN ZINC ION × 9 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7ZJX Rabbit 80S ribosome programmed with SECIS and SBP2 Deposited 2022-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 83-meric
Chain Sl 1–25（25 aa）
Not recorded ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8B5L Cryo-EM structure of ribosome-Sec61-TRAP (TRanslocon Associated Protein) translocon complex Deposited 2022-09-23 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
8B6C Cryo-EM structure of ribosome-Sec61 in complex with cyclotriazadisulfonamide derivative CK147 Deposited 2022-09-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 178 ZN ZINC ION × 5 OWI 4-[[9-(cyclohexylmethyl)-3-methylidene-5-(4-methylphenyl)sulfonyl-1,5,9-triazacyclododec-1-yl]sulfonyl]-~{N},~{N}-dimethyl-aniline × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain a1 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BPO Structure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER) Deposited 2022-11-17 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain m2 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 214 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Az 1–25（25 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain l 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain l 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Az 1–25（25 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8Q7Z Structure of the G. gallus 80S non-rotated ribosome Deposited 2023-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 80-meric
Chain Az 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 494 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8Q87 Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1 Deposited 2023-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain Az 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 492 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.40 Å
8RJB Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-RAMP4 Deposited 2023-12-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 51-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.69 Å
8RJC Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 1 Deposited 2023-12-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8RJD Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-TRAP, open conformation 2 Deposited 2023-12-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.79 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain MA 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AL41 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AL41 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDP 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla Deposited 2024-04-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain AL41 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain l 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Az 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Az 1–25（25 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Az 1–25（25 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain l 1–25（25 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain n 1–25（25 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain n 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Az 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Az 1–25（25 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain l1 1–25（25 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9UZK EMCV IRES captured on mammalian 40S with initiator tRNA Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain l 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.60 Å
9UZL EMCV IRES captured on mammalian 40S ribosome with initiator tRNA and eIF2 Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain l 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 5.01 Å
9UZM 40S ribosome without initiation factors Deposited 2025-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain l 1–25（25 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；20mM HEPES, 150mM KOAc, 2mM MgCl2, 1mM DTT, 0.5mM Spermidine, 6mM GMP-PnP
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 4.50 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain n 1–25（25 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

114 other PDB entries and 114 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name A0A087WNH4_RABIT
Isoform —
PDB entities 40
Chains and sequence ranges Author chain l； PDBConstruct 1–25； UniProt 1–25

Eukaryotic translation initiation factor 3 subunit D

OrganismNot specified

UniProt G1SQV5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain m； UniProt 1–557 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain D 1–557（557 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SQV5_RABIT
Isoform —
PDB entities 41
Chains and sequence ranges Author chain m； PDBConstruct 1–557； UniProt 1–557

40S ribosomal protein S25

OrganismNot specified

UniProt G1TDB3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain n； UniProt 1–124 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JAG Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
3JAH Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UAG stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.45 Å
3JAI Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon Deposited 2015-06-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 195 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer 50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT；pH 7.4；50 mM HEPES, 100 mM potassium acetate, 5 mM magnesium acetate, 1 mM DTT
cryo-EM vitrification conditions After 30 second wait time, blot for 3 seconds before plunging；Cryogen ETHANE；After 30 second wait time, blot for 3 seconds before plunging into liquid ethane (FEI VITROBOT MARK III).
Resolution 3.65 Å
4D5L Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state Deposited 2014-11-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain Z 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP；pH 7.5；20 MM TRIS PH 7.5, 100 MM KCL, 1 MM DTT, 2.5 MM MGCL2, 0.5 MM GTP
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 9.00 Å
4KZX Rabbit 40S ribosomal subunit in complex with eIF1. Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 35-meric
Chain Z 1–125（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.81 Å R-free 0.347
4KZY Rabbit 40S ribosomal subunit in complex with eIF1 and eIF1A. Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 36-meric
Chain Z 1–125（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.01 Å R-free 0.348
4KZZ Rabbit 40S ribosomal subunit in complex with mRNA, initiator tRNA and eIF1A Deposited 2013-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain Z 1–125（125 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 8.5；292 K；0.1M Ammonium acetate, 0.05M Tris-HCl pH 8.5, 2.5% PEG-20K, 4-6% MPD, 5.0mM Mg acetate, 2.0mM TCEP, vapor diffusion, temperature 292K
Resolution 7.03 Å R-free 0.359
5K0Y m48S late-stage initiation complex, purified from rabbit reticulocytes lysates, displaying eIF2 ternary complex and eIF3 i and g subunits relocated to the intersubunit face Deposited 2016-05-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain W 41–115（75 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5LZS Structure of the mammalian ribosomal elongation complex with aminoacyl-tRNA, eEF1A, and didemnin B Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 7C4 (2~{S})-~{N}-[(2~{R})-1-[[(3~{S},6~{S},8~{S},12~{S},13~{R},16~{S},17~{R},20~{S},23~{S})-13-[(2~{S})-butan-2-yl]-20-[(4-methoxyphenyl)methyl]-6,17,21-trimethyl-3-(2-methylpropyl)-12-oxidanyl-2,5,7,10,15,19,22-heptakis(oxidanylidene)-8-propan-2-yl-9,18-dioxa-1,4,14,21-tetrazabicyclo[21.3.0]hexacosan-16-yl]amino]-4-methyl-1-oxidanylidene-pentan-2-yl]-~{N}-methyl-1-[(2~{S})-2-oxidanylpropanoyl]pyrrolidine-2-carboxamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.31 Å
5LZT Structure of the mammalian ribosomal termination complex with eRF1 and eRF3. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 282 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.65 Å
5LZU Structure of the mammalian ribosomal termination complex with accommodated eRF1 Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 258 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.75 Å
5LZV Structure of the mammalian ribosomal termination complex with accommodated eRF1(AAQ) and ABCE1. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.35 Å
5LZW Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a truncated mRNA. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 281 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.53 Å
5LZX Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a UGA stop codon. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.67 Å
5LZY Structure of the mammalian rescue complex with Pelota and Hbs1l assembled on a polyadenylated mRNA. Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.99 Å
5LZZ Structure of the mammalian rescue complex with Pelota and Hbs1l (combined) Deposited 2016-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 264 ZN ZINC ION × 8 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 ul aliquots were applied to the grid and incubated for 30 s, before blotting for 3s to remove excess solution.
Resolution 3.47 Å
6D90 Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1. Deposited 2018-04-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain aa 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6D9J Mammalian 80S ribosome with a double translocated CrPV-IRES, P-sitetRNA and eRF1. Deposited 2018-04-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 85-meric
Chain aa 1–125（125 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6GZ3 tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1) Deposited 2018-07-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain BZ 28–113（86 aa）
Not recorded MG MAGNESIUM ION × 327 ZN ZINC ION × 5 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6HCF Structure of the rabbit 80S ribosome stalled on globin mRNA at the stop codon Deposited 2018-08-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain a1 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6HCJ Structure of the rabbit 80S ribosome on globin mRNA in the rotated state with A/P and P/E tRNAs Deposited 2018-08-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain a2 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6HCM Structure of the rabbit collided di-ribosome (stalled monosome) Deposited 2018-08-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 87-meric
Chain a1 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.80 Å
6HCQ Structure of the rabbit collided di-ribosome (collided monosome) Deposited 2018-08-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain a2 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 300 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.50 Å
6MTB Rabbit 80S ribosome with P- and Z-site tRNAs (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 83-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6MTC Rabbit 80S ribosome with Z-site tRNA and IFRD2 (unrotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6MTD Rabbit 80S ribosome with eEF2 and SERBP1 (unrotated state with 40S head swivel) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 297 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6MTE Rabbit 80S ribosome with eEF2 and SERBP1 (rotated state) Deposited 2018-10-19 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 298 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
6P4G Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P4H Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6P5I Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5J Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5K Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 3) Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 81-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5 to 3 s
Resolution 3.10 Å
6P5N Structure of a mammalian 80S ribosome in complex with a single translocated Israeli Acute Paralysis Virus IRES and eRF1 Deposited 2019-05-30 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 82-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Blot force = 3s Wait time = 15s Drain time = 0s Blot time = 2.5s
Resolution 3.20 Å
6R5Q Structure of XBP1u-paused ribosome nascent chain complex (post-state) Deposited 2019-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain OO 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6R6G Structure of XBP1u-paused ribosome nascent chain complex with SRP. Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 92-meric
Chain OO 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 299 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6R6P Structure of XBP1u-paused ribosome nascent chain complex (rotated state) Deposited 2019-03-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain OO 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 159 ZN ZINC ION × 5 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
6R7Q Structure of XBP1u-paused ribosome nascent chain complex with Sec61. Deposited 2019-03-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain OO 41–115（75 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 299 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6SGC Rabbit 80S ribosome stalled on a poly(A) tail Deposited 2019-08-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain a1 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 8 SPD SPERMIDINE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain a 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain i 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAL Mammalian 48S late-stage initiation complex with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 41-meric
Chain n 1–124（124 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain n 41–115（75 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6YAN Mammalian 48S late-stage translation initiation complex with histone 4 mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 41–115（75 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.48 Å
6ZVK The Halastavi arva virus (HalV) intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-07-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain M3 41–115（75 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.49 Å
7A01 The Halastavi arva virus intergenic region IRES promotes translation by the simplest possible initiation mechanism Deposited 2020-08-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 85-meric
Chain M3 41–115（75 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7JQB SARS-CoV-2 Nsp1 and rabbit 40S ribosome complex Deposited 2020-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 34-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
7JQC SARS-CoV-2 Nsp1, CrPV IRES and rabbit 40S ribosome complex Deposited 2020-08-10 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain a 1–125（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
7MDZ 80S rabbit ribosome stalled with benzamide-CHX Deposited 2021-04-06 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 81-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 277 ZN ZINC ION × 8 Z2V N-[(1R,3S,4R,5S)-3-{(1R)-2-[(2R,4r,6S)-2,6-dihydroxypiperidin-4-yl]-1-hydroxyethyl}-4-hydroxy-1,5-dimethylcyclohexyl]benzamide × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；3 sec blot
Resolution 3.20 Å
7NWH Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S. Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 84-meric
Chain ZZ 3–124（122 aa）
Not recorded ZN ZINC ION × 8 MG MAGNESIUM ION × 281 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 4.10 Å
7NWI Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S Deposited 2021-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain ZZ 3–124（122 aa）
Not recorded MG MAGNESIUM ION × 210 ZN ZINC ION × 5 BLS BLASTICIDIN S × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
cryo-EM vitrification conditions Cryogen ETHANE；30s sample incubation on grid followed by 1.1s blotting time.
Resolution 3.13 Å
7O7Y Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (high resolution) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ay 1–124（124 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 330 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.20 Å
7O7Z Rabbit 80S ribosome stalled close to the mutated SARS-CoV-2 slippery site by a pseudoknot (classified for pseudoknot) Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain Ay 1–124（124 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 329 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.40 Å
7O80 Rabbit 80S ribosome in complex with eRF1 and ABCE1 stalled at the STOP codon in the mutated SARS-CoV-2 slippery site Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain Ay 1–124（124 aa）
Not recorded SPD SPERMIDINE × 1 MG MAGNESIUM ION × 355 UNX UNKNOWN LIGAND × 374 ZN ZINC ION × 8 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
7O81 Rabbit 80S ribosome colliding in another ribosome stalled by the SARS-CoV-2 pseudoknot Deposited 2021-04-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain Ay 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 349 UNX UNKNOWN LIGAND × 266 ZN ZINC ION × 8 SPD SPERMIDINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.10 Å
7OYD Cryo-EM structure of a rabbit 80S ribosome with zebrafish Dap1b Deposited 2021-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ZZ 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 201 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.30 Å
7SYG Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 1(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.30 Å
7SYH Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 2(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYM Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 7(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.80 Å
7SYN Structure of the HCV IRES bound to the 40S ribosomal subunit, head opening. Structure 8(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYO Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYP Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.00 Å
7SYQ Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 37-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.80 Å
7SYR Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.60 Å
7SYS Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.50 Å
7SYT Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.40 Å
7SYU Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 37-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 4.60 Å
7SYV Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.90 Å
7SYW Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 NA SODIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7SYX Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) Deposited 2021-11-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 MG MAGNESIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；4 second blot time, force 3
Resolution 3.70 Å
7TOQ Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein poly-PR Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain AS25 41–115（75 aa）
Not recorded ZN ZINC ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7TOR Mammalian 80S ribosome bound with the ALS/FTD-associated dipeptide repeat protein GR20 Deposited 2022-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 86-meric
Chain AS25 41–115（75 aa）
Not recorded ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7UCJ Mammalian 80S translation initiation complex with mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 296 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
7UCK 80S translation initiation complex with ac4c(-1) mRNA and Harringtonine Deposited 2022-03-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 73 PDB declaration: 79-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 297 MQ6 Harringtonine × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
8BHF Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4 Deposited 2022-10-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain a3 40–114（75 aa）
Not recorded MG MAGNESIUM ION × 275 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8BTK Structure of the TRAP complex with the Sec translocon and a translating ribosome Deposited 2022-11-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 86 PDB declaration: 92-meric
Chain Ay 1–124（124 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 750 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE；3.5 uL of sample was incubated on the grid for 60 seconds at 4*C and 100% humidity before being blotted and plunged into liquid ethane/propane mix cooled to liquid nitrogen temperature
Resolution 3.50 Å
8P03 48S late-stage initiation complex with m6A mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain n 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 178 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.04 Å
8P09 48S late-stage initiation complex with non methylated mRNA Deposited 2023-05-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 40-meric
Chain n 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 230 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8P2K Ternary complex of translating ribosome, NAC and METAP1 Deposited 2023-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 82 PDB declaration: 88-meric
Chain Ay 1–124（124 aa）
Not recorded N ANY 5'-MONOPHOSPHATE NUCLEOTIDE × 73 SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 420 UNX UNKNOWN LIGAND × 312 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 AAC ACETYLAMINO-ACETIC ACID × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.90 Å
8SCB Terminating ribosome with SRI-41315 Deposited 2023-04-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 87-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 312 ZN ZINC ION × 8 ZVM (2S,4aS)-2-cyclobutyl-10-methyl-3-phenyl-2,10-dihydropyrimido[4,5-b]quinoline-4,5(3H,4aH)-dione × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VFT Translating 80S rabbit ribosome stalled by emetine with eEF2 Deposited 2023-12-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 85-meric
Chain ZZ 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 276 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 34G emetine × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
8VVP Codon sampling state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain ZB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 260 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 6 SPM SPERMINE × 2 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVQ Codon sampling state of elongation inhibitor-treated mammalian ribosomes obtained from merged datasets Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain ZB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 290 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 K POTASSIUM ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.70 Å
8VVR Post-decoding/Post-hydrolysis state obtained from Anisomycin-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain ZB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 204 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 3 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVS Post-decoding post-hydrolysis state obtained from merged datasets of elongation inhibitor-treated mammalian ribosomes Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 88-meric
Chain ZB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 236 ZN ZINC ION × 8 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 4 K POTASSIUM ION × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
8VVT Mammalian ribosomes bound to Anisomycin in the rotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 84-meric
Chain ZB 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 144 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
8VVU Anisomycin-bound mammalian ribosome with partially accommodated A-site tRNA Deposited 2024-01-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 86-meric
Chain ZB 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 102 ZN ZINC ION × 8 ANM ANISOMYCIN × 1 SPD SPERMIDINE × 1 SER SERINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
8VVV Mammalian ribosomes bound to Anisomycin in the nonrotated conformation Deposited 2024-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain ZZ 41–115（75 aa）
Not recorded MG MAGNESIUM ION × 294 ZN ZINC ION × 8 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM HEPES-KOH, pH 7.5, 100 mM KCl, 1.5 mM MgCl2, 0.5 mM Spermidine, 0.04 mM Spermine, 1 mM DTT
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9BDL 80S ribosome with angiogenin Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain AS25 41–115（75 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
9BDN 80S ribosome with angiogenin and tRNAAla Deposited 2024-04-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 77 PDB declaration: 85-meric
Chain AS25 41–115（75 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9C8K Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome Deposited 2024-06-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 35-meric
Chain Z 1–125（125 aa）
Not recorded MG MAGNESIUM ION × 74 ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain n 1–124（124 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å
9F1B Mammalian ternary complex of a translating 80S ribosome, NAC and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 83 PDB declaration: 89-meric
Chain Ay 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 420 ZN ZINC ION × 8 UNX UNKNOWN LIGAND × 306 IHP INOSITOL HEXAKISPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.01 Å
9F1C Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 84 PDB declaration: 90-meric
Chain Ay 1–124（124 aa）
Not recorded UNX UNKNOWN LIGAND × 277 MG MAGNESIUM ION × 420 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.78 Å
9F1D Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E-HYPK Deposited 2024-04-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain Ay 1–124（124 aa）
Not recorded UNX UNKNOWN LIGAND × 295 SPD SPERMIDINE × 30 MG MAGNESIUM ION × 420 SPM SPERMINE × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.26 Å
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain n 1–124（124 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å
9MR4 NAC: Ribosome nascent chain complex(Oxa1L) Deposited 2025-01-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 78 PDB declaration: 84-meric
Chain OO 1–124（124 aa）
Not recorded ZN ZINC ION × 6 MG MAGNESIUM ION × 285 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9NDP Structure of stalled ribosome and nascent chain in complex with NMT2 and NAC Deposited 2025-02-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 81 PDB declaration: 87-meric
Chain OO 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 296 ZN ZINC ION × 6 COA COENZYME A × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.82 Å
9OGY Megrivirus E 3' internal ribosome entry site (IRES) RNA core region bound to rabbit ribosome Deposited 2025-05-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 34 PDB declaration: 36-meric
Chain a 1–124（124 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；20 mM Tris-HCl pH 7.5, 2 mM MgOAc2, 150 mM KCl
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
9Q7Q ABCE1-eRF1-RNC-AMD1C Deposited 2025-08-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 80 PDB declaration: 86-meric
Chain OO 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 291 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.86 Å
9QQA Ternary complex of translating ribosome, NAC and NMT1 Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 86-meric
Chain Ay 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 407 UNX UNKNOWN LIGAND × 187 SPD SPERMIDINE × 30 SPM SPERMINE × 3 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 2.80 Å
9QQB Quaternary complex of a translating ribosome, NAC, NMT1, and NatA Deposited 2025-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 84 PDB declaration: 89-meric
Chain Ay 1–124（124 aa）
Not recorded SPD SPERMIDINE × 30 SPM SPERMINE × 3 MG MAGNESIUM ION × 412 UNX UNKNOWN LIGAND × 168 ZN ZINC ION × 8 IHP INOSITOL HEXAKISPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 3.43 Å
9RHU Rabbit 80S ribosome in complex with eRF1-AAQ, stalled at the Stop codon in mutated F2A sequence Deposited 2025-06-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 81 PDB declaration: 88-meric
Chain k1 1–124（124 aa）
Not recorded MG MAGNESIUM ION × 367 ZN ZINC ION × 8 K POTASSIUM ION × 37 SPD SPERMIDINE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9YPG GTPBP1*GCP*Phe-tRNA*ribosome in the GTPase activation-like state, Structure III Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPO GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIa Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPS GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIb Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPT GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IIc Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9YPV GTPBP1*GCP*Phe-tRNA*ribosome in the open state, Structure IId Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPW GTPBP1*GDP*Phe-tRNA*ribosome in the post-GTP hydrolysis state, Structure IV Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 84-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 K POTASSIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YPY Ribosome with accommodated A-site tRNA, Structure V Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 83-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 PHE PHENYLALANINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9YPZ Vacant ribosome with P-site tRNA, substate 1, Structure Ia Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ0 Vacant ribosome with P-site tRNA, substate 2, Structure Ib Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
9YQ1 Vacant ribosome with P-site tRNA, substate 3, Structure Ic Deposited 2025-10-14 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Assembly 1 Protein–RNA Heteromer；Protein × 75 PDB declaration: 82-meric
Chain ZZ 1–125（125 aa）
Not recorded SPD SPERMIDINE × 1 ZN ZINC ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MET METHIONINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å

113 other PDB entries and 113 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TDB3_RABIT
Isoform —
PDB entities 42
Chains and sequence ranges Author chain n； PDBConstruct 1–124； UniProt 1–124

Eukaryotic translation initiation factor 5

OrganismNot specified

UniProt G1TGW1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain o； UniProt 1–430 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
9H6Y Late-stage 48S Initiation Complex (LS48S IC) guided by the trans-RNA Deposited 2024-10-25 Different oligomeric state Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 42-meric
Chain o 1–430（430 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
cryo-EM vitrification conditions Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5
Resolution 2.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1TGW1_RABIT
Isoform —
PDB entities 43
Chains and sequence ranges Author chain o； PDBConstruct 1–430； UniProt 1–430

Eukaryotic translation initiation factor 3 subunit F

OrganismNot specified

UniProt G1SLC2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain q； UniProt 1–364 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 4 1–364（364 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 4 1–364（364 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain q 93–364（272 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain r 1–364（364 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain q 1–364（364 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SLC2_RABIT
Isoform —
PDB entities 44
Chains and sequence ranges Author chain q； PDBConstruct 1–364； UniProt 1–364

Eukaryotic translation initiation factor 3 subunit H

OrganismNot specified

UniProt A0A1U7U441

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain r； UniProt 1–352 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

No other PDB entry for the same UniProt protein was found.

View Construct and Data Evidence
UniProt name A0A1U7U441_CARSF
Isoform —
PDB entities 45
Chains and sequence ranges Author chain r； PDBConstruct 1–352； UniProt 1–352

Eukaryotic translation initiation factor 3 subunit K

OrganismNot specified

UniProt G1T3L2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain s； UniProt 1–218 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain K 1–218（218 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 6 1–218（218 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 6 1–218（218 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain s 1–218（218 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain t 1–218（218 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain s 1–218（218 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1T3L2_RABIT
Isoform —
PDB entities 46
Chains and sequence ranges Author chain s； PDBConstruct 1–218； UniProt 1–218

Eukaryotic translation initiation factor 3 subunit L

OrganismNot specified

UniProt G1SED9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain t； UniProt 1–607 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain L 43–606（564 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 7 1–607（607 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 7 1–607（607 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain u 44–607（564 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain t 44–607（564 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SED9_RABIT
Isoform —
PDB entities 47
Chains and sequence ranges Author chain t； PDBConstruct 1–607； UniProt 1–607

Eukaryotic translation initiation factor 3 subunit M

OrganismNot specified

UniProt G1SLW8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain u； UniProt 1–374 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–374（374 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 8 1–374（374 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 8 1–374（374 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain u 1–374（374 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain v 1–374（374 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain u 1–374（374 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SLW8_RABIT
Isoform —
PDB entities 48
Chains and sequence ranges Author chain u； PDBConstruct 1–374； UniProt 1–374

Eukaryotic translation initiation factor 3 subunit C

OrganismNot specified

UniProt G1U971

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain v； UniProt 1–913 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 71–913（843 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 2 1–913（913 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 2 1–913（913 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain v 1–913（913 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain k 1–913（913 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1U971_RABIT
Isoform —
PDB entities 49
Chains and sequence ranges Author chain v； PDBConstruct 1–913； UniProt 1–913

Eukaryotic translation initiation factor 3 subunit E

OrganismNot specified

UniProt G1SUC8

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain w； UniProt 1–462 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit A × 1 （G1SMZ5） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 18–462（445 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 3 1–462（462 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 3 1–462（462 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
6YAM Mammalian 48S late-stage translation initiation complex (LS48S+eIF3 IC) with beta-globin mRNA Deposited 2020-03-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 50-meric
Chain w 18–462（445 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 2 MG MAGNESIUM ION × 1 GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain q 18–462（445 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9CPA Structure of a Mammalian DHX29-bound 43S Pre-initiation Complex Deposited 2024-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 51-meric
Chain Q 1–462（462 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE-PROPANE
Resolution 6.00 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SUC8_RABIT
Isoform —
PDB entities 50
Chains and sequence ranges Author chain w； PDBConstruct 1–462； UniProt 1–462

Eukaryotic translation initiation factor 3 subunit A

OrganismNot specified

UniProt G1SMZ5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 3 PDB declaration: 51-meric（51） Consistent with all polymer counts Chain y； UniProt 1–1362 Not recorded Initiator methionylated tRNA × 1 18S ribosomal RNA × 1 Linear mRNA × 1 Eukaryotic translation initiation factor 2 subunit 1 × 1 （G1T2G4） protein-synthesizing GTPase × 1 （A0A5F9C3W2） 40S ribosomal protein SA × 1 （G1TLT8） 40S ribosomal protein S3a × 1 （G1TN72） Small ribosomal subunit protein uS5 × 1 （A0AAA9WW19） Ribosomal protein S3 × 1 （G1TNM3） 40S ribosomal protein S4 × 1 （G1TK17） Ribosomal protein S5 × 1 （G1TFM5） Small ribosomal subunit protein eS6 × 1 （G1TM55） 40S ribosomal protein S7 × 1 （G1SVB0） Small ribosomal subunit protein eS8 × 1 （G1TJW1） 40S ribosomal protein S9 × 1 （B7NZS8） Small ribosomal subunit protein eS10 × 1 （G1U0Y5） 40S ribosomal protein S11 × 1 （G1TRM4） 40S ribosomal protein S12 × 1 （G1SFR8） 40S ribosomal protein S13 × 1 （G1SP51） 40S ribosomal protein uS11 × 1 （G1T1F0） 40S ribosomal protein uS19 × 1 （G1U0Q2） Ribosomal protein S16 × 1 （G1SGX4） 40S ribosomal protein eS17 × 1 （G1TU13） 40S ribosomal protein uS13 × 1 （G1TPG3） 40S ribosomal protein eS19 × 1 （G1TN62） 40S ribosomal protein uS10 × 1 （G1SIZ2） 40S ribosomal protein S21 × 1 （Q32PB8） Ribosomal protein S15a × 1 （G1TG89） 40S ribosomal protein S23 × 1 （G1SZ47） 40S ribosomal protein S24 × 1 （G1TS40） 40S ribosomal protein S26 × 1 （G1TFE8） 40S ribosomal protein S27 × 1 （G1TZ76） 40S ribosomal protein S28 × 1 （G1TIB4） 40S ribosomal protein S29 × 1 （G1U7M4） Ribosomal protein S27a × 1 （G1SK22） Small ribosomal subunit protein RACK1 × 1 （G1SJB4） 40S ribosomal protein S30 × 1 （G1T8A2） Eukaryotic translation initiation factor 1A, X-chromosomal × 1 （P47813） ATP binding cassette subfamily E member 1 × 1 （A0A5K1V8K4） 60S ribosomal protein L41 × 1 （A0A087WNH4） Eukaryotic translation initiation factor 3 subunit D × 1 （G1SQV5） 40S ribosomal protein S25 × 1 （G1TDB3） Eukaryotic translation initiation factor 5 × 1 （G1TGW1） Eukaryotic translation initiation factor 3 subunit F × 1 （G1SLC2） Eukaryotic translation initiation factor 3 subunit H × 1 （A0A1U7U441） Eukaryotic translation initiation factor 3 subunit K × 1 （G1T3L2） Eukaryotic translation initiation factor 3 subunit L × 1 （G1SED9） Eukaryotic translation initiation factor 3 subunit M × 1 （G1SLW8） Eukaryotic translation initiation factor 3 subunit C × 1 （G1U971） Eukaryotic translation initiation factor 3 subunit E × 1 （G1SUC8） SF4 IRON/SULFUR CLUSTER × 2 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail cryo-EM vitrification conditions：Cryogen ETHANE；The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5 Resolution 2.90 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 9H74 Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5A5T Structure of mammalian eIF3 in the context of the 43S preinitiation complex Deposited 2015-06-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–1362（1362 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,
Resolution 6.00 Å
6W2S Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 43-meric
Chain 1 1–1362（1362 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.47 Å
6W2T Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the closed state (Class 2) Deposited 2020-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 44-meric
Chain 1 1–1362（1362 aa）
Not recorded MG MAGNESIUM ION × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2.5s and flash cooled in liquid ethane
Resolution 3.36 Å
8SUP Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES Deposited 2023-05-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 44 PDB declaration: 47-meric
Chain o 1–1362（1362 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å

4 other PDB entries and 4 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name G1SMZ5_RABIT
Isoform —
PDB entities 51
Chains and sequence ranges Author chain y； PDBConstruct 1–1362； UniProt 1–1362

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 9h74

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 9h74
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2. Structure Basics 2. Structure Basics

Entry ID entry_id9h74
Deposition date deposition_date2024-10-25
最后修订 last_revision2025-12-10
Structure title titleLate-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA
Keywords keywords48S late-stage initiation complex, Programmable mRNA translation initiation, trans-RNA, activation of mRNA translation, TRANSLATION; TRANSLATION
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier91.80 Å
Radius of gyration Rg (electron density) rg_electron95.35 Å
Forward intensity I(0) i064718700000.00
Molecular weight molecular_weight1729000.0 kDa
Excluded volume excluded_volume1983100 ų
Envelope volume envelope_volume3712800 ų
Hydration-shell volume shell_volume319080 ų
Envelope diameter envelope_diameter326.8 Å
Shell Rg shell_rg93.46 Å
Envelope Rg envelope_rg93.73 Å
Shape Rg shape_rg95.50 Å
Total Rg total_rg94.96 Å
Total atoms total_atoms118867
Residues n_residues11785
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax243.2 Å
Rg (real space) rg_real88.02 Å
Rg uncertainty (real space) rg_real_error0.66 Å
I(0) (real space) i0_real6.1900e+10
I(0) uncertainty (real space) i0_real_error1.1850e+09
Rg (reciprocal space) rg_reciprocal90.77 Å
I(0) (reciprocal space) i0_reciprocal64500000000.0000
Solution quality estimate total_estimate0.9146
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary100.1 Å
Skewness Skewness skewness0.248
Kurtosis Kurtosis kurtosis-0.631
Angular range angular_range— – 0.0850 Å−1
Current regularization parameter α current_alpha0.7235
Highest regularization parameter α highest_alpha3590000000.0000
Real-space data points n_real_points18
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.999; Stabil: 0.966; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (52)

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8. Citations (1)

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9. Files and Curves (10)