9ha3

Pooled 50S subunit C_(L22)-~H61 precursor states supplemented with Api137

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Large ribosomal subunit protein bL34

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL34_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain 2； PDBConstruct 1–46； UniProt 1–46

Large ribosomal subunit protein uL13

OrganismNot specified

UniProt P0AA10

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL13_ECOLI
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain J； PDBConstruct 1–142； UniProt 1–142

Large ribosomal subunit protein bL20

OrganismNot specified

UniProt P0A7L3

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL20_ECOLI
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain Q； PDBConstruct 1–117； UniProt 2–118

Large ribosomal subunit protein bL21

OrganismNot specified

UniProt P0AG48

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL21_ECOLI
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain R； PDBConstruct 1–103； UniProt 1–103

Large ribosomal subunit protein uL23

OrganismNot specified

UniProt P0ADZ0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL23_ECOLI
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain T； PDBConstruct 1–93； UniProt 1–93

Large ribosomal subunit protein uL24

OrganismNot specified

UniProt P60624

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL24_ECOLI
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain U； PDBConstruct 1–102； UniProt 2–103

Large ribosomal subunit protein uL29

OrganismNot specified

UniProt P0A7M6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL29_ECOLI
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain Y； PDBConstruct 1–63； UniProt 1–63

Large ribosomal subunit protein uL30

OrganismNot specified

UniProt P0AG51

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL30_ECOLI
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain Z； PDBConstruct 1–58； UniProt 2–59

50S ribosomal protein L3

OrganismNot specified

UniProt P60438

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL3_ECOLI
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain D； PDBConstruct 1–209； UniProt 1–209

Large ribosomal subunit protein uL4

OrganismNot specified

UniProt P60723

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL4_ECOLI
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain E； PDBConstruct 1–201； UniProt 1–201

Large ribosomal subunit protein uL15

OrganismNot specified

UniProt P02413

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein bL17 × 1 （P0AG44） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL15_ECOLI
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain L； PDBConstruct 1–143； UniProt 2–144

Large ribosomal subunit protein bL17

OrganismNot specified

UniProt P0AG44

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Apidaecins type 22 × 1 （P35581）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RL17_ECOLI
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain N； PDBConstruct 1–120； UniProt 1–120

Apidaecins type 22

OrganismNot specified

UniProt P35581

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 1	Large ribosomal subunit protein bL34 × 1 （P0A7P5） Large ribosomal subunit protein uL13 × 1 （P0AA10） Large ribosomal subunit protein bL20 × 1 （P0A7L3） Large ribosomal subunit protein bL21 × 1 （P0AG48） Large ribosomal subunit protein uL23 × 1 （P0ADZ0） Large ribosomal subunit protein uL24 × 1 （P60624） Large ribosomal subunit protein uL29 × 1 （P0A7M6） Large ribosomal subunit protein uL30 × 1 （P0AG51） 23S ribosomal RNA × 1 50S ribosomal protein L3 × 1 （P60438） Large ribosomal subunit protein uL4 × 1 （P60723） Large ribosomal subunit protein uL15 × 1 （P02413） Large ribosomal subunit protein bL17 × 1 （P0AG44）	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	APD22_APIME
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain y； PDBConstruct 1–17； UniProt 44–60

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9ha3
Deposition date deposition_date	2024-11-01
Last revision last_revision	2025-01-22
Structure title title	Pooled 50S subunit C_(L22)-~H61 precursor states supplemented with Api137
Keywords keywords	ribosome, antimicrobial peptide, RNA, ribosomal protein, PrAMP, proline-rich peptide, antibiotics, 50S, Api137; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9ha3__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9ha3__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9ha3__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	62.33 Å
Rg (electron density)	62.36 Å
Total Rg	62.46 Å
Atom count	52595
Residues	3292
Excluded volume	780600 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9ha3__assembly_1__model_1`	14-meric （14）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (14)

Entity 1 Entity 1 polymer

Description description	Large ribosomal subunit protein bL34
Molecular weight formula_weight	5397.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	2
Atom count atom_count	52595

MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK

Entity 2 Entity 2 polymer

Description description	Large ribosomal subunit protein uL13
Molecular weight formula_weight	16050.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI

Entity 3 Entity 3 polymer

Description description	Large ribosomal subunit protein bL20
Molecular weight formula_weight	13396.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA

Entity 4 Entity 4 polymer

Description description	Large ribosomal subunit protein bL21
Molecular weight formula_weight	11586.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

Entity 5 Entity 5 polymer

Description description	Large ribosomal subunit protein uL23
Molecular weight formula_weight	10546.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNL

Entity 6 Entity 6 polymer

Description description	Large ribosomal subunit protein uL24
Molecular weight formula_weight	11078.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

AAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSETI

Entity 7 Entity 7 polymer

Description description	Large ribosomal subunit protein uL29
Molecular weight formula_weight	7286.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA

Entity 8 Entity 8 polymer

Description description	Large ribosomal subunit protein uL30
Molecular weight formula_weight	6423.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

AKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE

Entity 9 Entity 9 polymer

Description description	23S ribosomal RNA
Molecular weight formula_weight	941619.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	A

GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCGGUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGAAUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCCAGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGAUACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUGAAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAAGAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGCGUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUCUUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACUAACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUAUAAGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUCUGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUCGUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAACGGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCAUUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAAGGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACCUU

Entity 10 Entity 10 polymer

Description description	50S ribosomal protein L3
Molecular weight formula_weight	22277.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA

Entity 11 Entity 11 polymer

Description description	Large ribosomal subunit protein uL4
Molecular weight formula_weight	22121.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLAARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA

Entity 12 Entity 12 polymer

Description description	Large ribosomal subunit protein uL15
Molecular weight formula_weight	14877.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

RLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Entity 13 Entity 13 polymer

Description description	Large ribosomal subunit protein bL17
Molecular weight formula_weight	13721.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSE

Entity 14 Entity 14 polymer

Description description	Apidaecins type 22
Molecular weight formula_weight	2084.5 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	y

NNRPVYIPRPRPPHPRL

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	3.62 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	50896

Pooled 50S subunit C_(L22)-~H61 precursor states supplemented with Api137

1. Protein Identity and Related Structures Protein Identity & Related Structures

Large ribosomal subunit protein bL34

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL13

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein bL20

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein bL21

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL23

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL24

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL29

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL30

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

50S ribosomal protein L3

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Large ribosomal subunit protein uL4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer