SAXSdb — 9oz1

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9oz1
沉积日期 deposition_date	2025-06-04
结构标题 title	;Cryo-EM structure of chicken ROS1 in apo-state. Cryo-EM refinement is focused on the "leg" region of chicken ROS1. ;
关键词 keywords	ROS1, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	58.28 Å
回转半径 Rg (电子) rg_electron	59.72 Å
零角强度 I(0) i0	119092000.00
分子量 molecular_weight	92509.0 kDa
排除体积 excluded_volume	116640 Å³
包络体积 envelope_volume	188820 Å³
水化壳体积 shell_volume	30739 Å³
包络直径 envelope_diameter	202.0 Å
壳层 Rg shell_rg	49.63 Å
包络 Rg envelope_rg	59.79 Å
形状 Rg shape_rg	59.70 Å
总 Rg total_rg	59.43 Å
总原子数 total_atoms	12962
残基数 n_residues	780
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	218.3 Å
Rg (实空间) rg_real	59.90 Å
Rg 误差 (实空间) rg_real_error	4.36 Å
I(0) (实空间) i0_real	1.1910e+08
I(0) 误差 (实空间) i0_real_error	2.9830e+06
Rg (倒空间) rg_reciprocal	56.87 Å
I(0) (倒空间) i0_reciprocal	118500000.0000
解质量估计 total_estimate	0.5742
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	32.3 Å
偏度 Skewness skewness	0.618
峰度 Kurtosis kurtosis	-0.728
角度范围 angular_range	— – 0.1350 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	4327000.0000
实空间数据点数 n_real_points	28
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.037; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.004; Smooth: 0.349

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (4)

Entity 1 polymer

描述 description	Tyrosine-protein kinase receptor
分子量 formula_weight	261174.8 kDa
EC 编号 ec_number	2.7.10.1
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	12962

MRNACLLLNRLGAFYFIWISAAYCSFSKNCQDLCTSNLEGELGIANLCNVSDINVACTQGCQFWNATEQVNCPLKCNKTYTRECETVSCKFGCSRAEDAYGVEAQNCLNKPGAPFASSIGSHNITLGWKPANISEVKYIIQWKFHQLPGDWRYTEVVSETSYTVKDLQAFTEYEFRVVWIITSQLQLHSPPSPSYRTHASGVPTTAPIIKDIQSSSPNTVEVSWFPPLFPNGLIVGYNLVLTSENHELLRASRGHSFQFYSTFPNSTYRFSIVAVNEAGAGPPAEANITTPESKVKEKAKWLFLSRNQSLRKRYMEHFLEAAHCLQNGIIHHNITGISVNVYQQVVYFSEGNSIWVKGVVDMSDVSDLTLFYTGWGNITSISVDWLYQRMYFVMNEKIHVCQLENCTAAEDITPPYVTSPRKIVADPYNGYIFCLLEDGIYRANLPLFPDTASAASLVVKSHTLRDFMINFQSKRLIFFNKTEQAFVSGFLDGSEFHTLRAHVPLDDMESFVYEDNIFTVTDGRAVFHEEISQVGSSSFNEYVVDCSLEYPEYFGFGNLLFYAASTQPYPLPTLPRLVTVLFGSDQAVISWSPPEYTIGTSRSAWQNWTYDVKVSSQSTFEEEWVVSNITDTRFAVKNLVSFTEYEMSVRAVSPAGEGPWSEPFRGMTFEEAEEEPYILAVGAEGLWKQRLDSYGPGEFLYPHIRNISDLDWYNDTLYWSNSMGKVQTWSMNKKEGTTENSYVPDIKNARMLAFDWLGQCLYWAGKANTIYRKSLLGDHMDVVAHVVYVVKDLAVDSVNGYLYWATTYTVESARLNGEEYLILQEHLQFSGKQVVGLALDLTSGFLYWLVQDGLCLNLYRISICKESCGNIMVTEISAWSVSEVSQNALQYYSGRLFWINRLKFITTQELNQSISIPFSEPAEFAAFTLVHTSLKPLPGNFSFTPKVIPSSVPESSFKIKGNSSSFHIIWNASTDVQWGTVFYCVGSNALQMRTLESERCLHPHDLTVPSYKVDWLEPFTLFDFSVTPYTYWGKAPTTSVYLRAPEGVPSAPANPRIYVLHSNTHEGEEKVLVELRWDKPERDNGVLTQFRVYYQLLYESGAADTLMEWNVSDVKPTALLFSIRDVHPRLTVRFQVQAFTSVGPGPMSDVAQRNSSDIFPVPTLITFSSNKLFLTDIDSNHTIWEVLTNRNIKDICYTADDDKVYYILEDSLFLLNVQSTSESQLFEDVFLRNVTAITVDWIARHLFVAMKTSWNETQVFFIDLELKTKSLKALNIQLGKRNSTISSLLSYPFLSRLYWIEELDYGSRMFYYDILNNTMYHILGYESVEEKMRNYCNCNVAEAELGRPISIDVTDIKKPQLLFIRGRDEIWASDVDGCHCWRITKIPSFQGTKIGSLTVDKQFIYWTIEKKEYTEICLADKESTRHSLQRKANHELKILAYSSAMQSYPDKKCLTPLLDTEKPTILDTTNTSFTLSLPSVTTQQLCPSISQPTPTYLVFFREITSNHENSTYHFSTLLQKTLEIQEPIAVINNLKPFSSYAIQVAVKNYYSNQNQLAVGRETISTTLYGVPEGVDSIKTVVLSDTTINISWSEPLEPNGPLESIRYQISVNLLSLFPEAPLRKSEFPNGTLSWSVSDLQSGTNNLFKVLAFHPNENWFSESVPVIAKTFETPLSPSNIIPRNTSFQLEWRAPLHINGTSFWFELRKWQTRSDWFSPASTTCTVGPVYTCNLTGTLPSANYLVRATVVYVTGMKSTSSPTSFKTTAGVPSKPGTPKRAEDSKNSVQWEKAEDNGSNLTYYILESRKQSGNTNKVKSLWVVVYNGSCDSICTWKAENLEGTFQFRAAAANMLGLGEYSDTSKDIVLAKDTVTSPDITAIVAVIGAVVLGLTIIILFGFVWHQRWKSRKPASTGQIVLVKEDKELAQLRGMAETVGLANACYAVSTLPSQAEIESLPAFPRDKLNLHKLLGSGAFGEVYEGTALDILADGSGESRVAVKTLKRGATDQEKSEFLKEAHLMSKFDHPHILKLLGVCLLNEPQYLILELMEGGDLLSYLRGARKQKFQSPLLTLTDLLDICLDICKGCVYLEKMRFIHRDLAARNCLVSEKQYGSCSRVVKIGDFGLARDIYKNDYYRKRGEGLLPVRWMAPESLIDGVFTNHSDVWAFGVLVWETLTLGQQPYPGLSNIEVLHHVRSGGRLESPNNCPDDIRDLMTRCWAQDPHNRPTFFYIQHKLQEIRHSPLCFSYFLGDKESVAGFINQAFEDIDVPPADSDSILSTTLMEARDQEGLNYLVVVKESNQDQGSISSAELTSV

来源 Source

来源生物 organism	Gallus gallus
通用名 common_name	chicken
NCBI 分类编号 ncbi_taxonomy_id	9031
宿主生物 host_organism	Homo sapiens

Entity 2 branched

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	424.4 kDa
来源方法 src_method	man
拷贝数 entity_count	3

Entity 3 branched

描述 description	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	586.5 kDa
来源方法 src_method	man
拷贝数 entity_count	1

Entity 4 non-polymer

描述 description	2-acetamido-2-deoxy-beta-D-glucopyranose
分子量 formula_weight	221.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	10

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	3.600 Å
最低分辨率 resolution_low	— Å
R 因子 r_factor	—
R_work r_work	—
R_free r_free	—

分辨率 resolution	3.60 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	255602

fit	/app/data/saxs_computation/oz/9oz1/9oz1.fit
pddf	/app/data/pr_computation/oz/9oz1/9oz1.pr.out
profile	/app/data/saxs_computation/oz/9oz1/9oz1.dat

;Cryo-EM structure of chicken ROS1 in apo-state. Cryo-EM refinement is focused on the "leg" region of chicken ROS1. ;

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D