Fem-1 homolog B (FEM1B) in complex with VU0417412

Method: X-RAY DIFFRACTION D_max: 115.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9pw8
deposition_date	2025-08-04
last_revision	2025-11-26
title	Fem-1 homolog B (FEM1B) in complex with VU0417412
keywords	FEM1B, E3 ligase, ligand, LIGASE; LIGASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.50 Å
rg_electron	32.57 Å
i0	156044000.00
molecular_weight	66466.0 kDa
excluded_volume	64078 Å³
envelope_volume	116510 Å³
shell_volume	31542 Å³
envelope_diameter	123.7 Å
shell_rg	37.03 Å
envelope_rg	32.37 Å
shape_rg	32.59 Å
total_rg	32.79 Å
total_atoms	5034
n_residues	669
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	115.8 Å
rg_real	32.78 Å
rg_real_error	1.52 Å
i0_real	1.5600e+08
i0_real_error	2.7540e+06
rg_reciprocal	32.66 Å
i0_reciprocal	156000000.0000
total_estimate	0.8439
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	35.5 Å
skewness	0.483
kurtosis	-0.268
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	16470000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.757; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.734; Smooth: 0.964

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	123.708 Å
length_b	123.708 Å
length_c	137.786 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 4 21 2
int_tables_number	90
crystal_system	tetragonal

resolution	2.800 Å
resolution_low	46.030 Å
r_factor	0.3014
r_work	0.2994
r_free	0.3378

description	Protein fem-1 homolog B
formula_weight	39295.8 kDa
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	A,B
atom_count	5034

Fem-1 homolog B (FEM1B) in complex with VU0417412

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

description	N-(4-fluorophenyl)thiophene-2-carboxamide
formula_weight	221.3 kDa
src_method	syn
entity_count	2

fit	/app/data/saxs_computation/pw/9pw8/9pw8.fit
pddf	/app/data/pr_computation/pw/9pw8/9pw8.pr.out
profile	/app/data/saxs_computation/pw/9pw8/9pw8.dat