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9qm2

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis

Method: X-RAY DIFFRACTION D_max: 81.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qm2
deposition_date	2025-03-21
title	Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis
keywords	Monooxygenase, FAD-binding, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	25.08 Å
rg_electron	24.10 Å
i0	58243100.00
molecular_weight	57482.0 kDa
excluded_volume	71220 Å³
envelope_volume	83873 Å³
shell_volume	29000 Å³
envelope_diameter	84.3 Å
shell_rg	31.75 Å
envelope_rg	24.37 Å
shape_rg	24.10 Å
total_rg	24.94 Å
total_atoms	4053
n_residues	536
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	81.5 Å
rg_real	25.03 Å
rg_real_error	0.40 Å
i0_real	5.8240e+07
i0_real_error	8.6590e+05
rg_reciprocal	25.05 Å
i0_reciprocal	58240000.0000
total_estimate	0.8939
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.8 Å
skewness	0.310
kurtosis	-0.333
angular_range	— – 0.3150 Å⁻¹
current_alpha	0.0000
highest_alpha	11590000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.876; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.992

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	FAD dependent monooxygenase
formula_weight	59508.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4147

MGSSHHHHHHSSGLVPRGSHMPAPDETVPVLVAGAGPGGLSTAVFLGLHGVPALVVERHPGTSTAVKATGQYPHTMEALAIAGAAGTVRERGRAYRSDFHMVVAKTLAGPVLRTLMSGDQLSMRHVSPEDWGTASQSAVESVLADRAAELGSRLRFSTRLTSLTQDADGVTAVTQHTGTGERRVIRARYLVVADGWRSGIRQSLGIEMRGRGTVGKVLRVLFEADLSEPLSHTDGAADGRRFTALHVGRAVLFNTEIPGLYGYFRNLTPELPDGWWTNEDTVAAQIRSDLGIPDIPLKIEEISETEISCGVAERFREGRALLVGDAAHVMPPTGGMGGNTAYLDGLYLGWKLAAVLRGTAGEALLDSHDAERRPYAEELVEQQFANLVDRISPELADESLAVALPPPVVAFGYRFPKGAVLLEPDDDGELFEDPSRPTGRPGSHAPYVPLTRRDGSATSTTALFGHAFVLLTGPDGAAWAEGALASADALGVAVQVHRVGPGSELLDAEGAFSTAYGIGADGAALVRPDRFVAWRCTGTHPDPAAEIERALRHVLHRPAH

Source

organism	Streptomyces bottropensis
ncbi_taxonomy_id	42235
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	CHLORIDE ION
formula_weight	35.5 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	66

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6. Citations (1)

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length_a	111.670 Å
length_b	118.230 Å
length_c	104.460 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	orthorhombic

resolution	2.400 Å
resolution_low	49.240 Å
r_factor	0.2133
r_work	0.2110
r_free	0.2578

fit	/app/data/saxs_computation/qm/9qm2/9qm2.fit
pddf	/app/data/pr_computation/qm/9qm2/9qm2.pr.out
profile	/app/data/saxs_computation/qm/9qm2/9qm2.dat

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction