← Back to search

9qm4

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative N-terminus)

Method: X-RAY DIFFRACTION D_max: 81.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9qm4
deposition_date	2025-03-21
title	Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative N-terminus)
keywords	Monooxygenase, FAD-binding, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	25.17 Å
rg_electron	24.19 Å
i0	59161500.00
molecular_weight	58031.0 kDa
excluded_volume	71943 Å³
envelope_volume	85468 Å³
shell_volume	29377 Å³
envelope_diameter	84.2 Å
shell_rg	31.90 Å
envelope_rg	24.46 Å
shape_rg	24.19 Å
total_rg	25.03 Å
total_atoms	4094
n_residues	542
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	81.2 Å
rg_real	25.12 Å
rg_real_error	0.42 Å
i0_real	5.9160e+07
i0_real_error	7.1170e+05
rg_reciprocal	25.14 Å
i0_reciprocal	59160000.0000
total_estimate	0.8956
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	29.6 Å
skewness	0.296
kurtosis	-0.345
angular_range	— – 0.3150 Å⁻¹
current_alpha	0.0000
highest_alpha	11250000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.886; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.983

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	FAD dependent monooxygenase
formula_weight	59849.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4110

STQRARRPDPAAPPTPVPAPAPAPAPAPAPDETVPVLVAGAGPGGLSTAVFLGLHGVPALVVERHPGTSTAVKATGQYPHTMEALAIAGAAGTVRERGRAYRSDFHMVVAKTLAGPVLRTLMSGDQLSMRHVSPEDWGTASQSAVESVLADRAAELGSRLRFSTRLTSLTQDADGVTAVTQHTGTGERRVIRARYLVVADGWRSGIRQSLGIEMRGRGTVGKVLRVLFEADLSEPLSHTDGAADGRRFTALHVGRAVLFNTEIPGLYGYFRNLTPELPDGWWTNEDTVAAQIRSDLGIPDIPLKIEEISETEISCGVAERFREGRALLVGDAAHVMPPTGGMGGNTAYLDGLYLGWKLAAVLRGTAGEALLDSHDAERRPYAEELVEQQFANLVDRISPELADESLAVALPPPVVAFGYRFPKGAVLLEPDDDGELFEDPSRPTGRPGSHAPYVPLTRRDGSATSTTALFGHAFVLLTGPDGAAWAEGALASADALGVAVQVHRVGPGSELLDAEGAFSTAYGIGADGAALVRPDRFVAWRCTGTHPDPAAEIERALRHVLHRPAH

Source

organism	Streptomyces bottropensis
ncbi_taxonomy_id	42235
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	10

▶

6. Citations (1)

▶

length_a	112.578 Å
length_b	115.998 Å
length_c	104.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	orthorhombic

resolution	2.700 Å
resolution_low	49.590 Å
r_factor	0.2228
r_work	0.2209
r_free	0.2581

fit	/app/data/saxs_computation/qm/9qm4/9qm4.fit
pddf	/app/data/pr_computation/qm/9qm4/9qm4.pr.out
profile	/app/data/saxs_computation/qm/9qm4/9qm4.dat

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative N-terminus)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction