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9qu1

Crystal structure of the human RalGAPA2 N-terminal domain with human kappaB-Ras1

Method: X-RAY DIFFRACTION D_max: 124.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9qu1
deposition_date	2025-04-09
title	Crystal structure of the human RalGAPA2 N-terminal domain with human kappaB-Ras1
keywords	RAL, GTPase, RalGAP, kB-Ras, SIGNALING PROTEIN; SIGNALING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	36.43 Å
rg_electron	36.24 Å
i0	118405000.00
molecular_weight	91820.0 kDa
excluded_volume	116800 Å³
envelope_volume	149020 Å³
shell_volume	36611 Å³
envelope_diameter	131.5 Å
shell_rg	39.55 Å
envelope_rg	36.15 Å
shape_rg	36.21 Å
total_rg	36.60 Å
total_atoms	12989
n_residues	784
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	124.8 Å
rg_real	36.81 Å
rg_real_error	1.51 Å
i0_real	1.1840e+08
i0_real_error	2.1750e+06
rg_reciprocal	36.58 Å
i0_reciprocal	118400000.0000
total_estimate	0.7876
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	31.9 Å
skewness	0.519
kurtosis	-0.470
angular_range	— – 0.2150 Å⁻¹
current_alpha	0.0000
highest_alpha	26230000.0000
n_real_points	44
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.662; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.590; Smooth: 0.658

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Ral GTPase-activating protein subunit alpha-2
formula_weight	29617.4 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	13021

FSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLKQFFETNYSQIYFIFYENFIALENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFP

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 2 polymer

description	NF-kappa-B inhibitor-interacting Ras-like protein 1
formula_weight	20121.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,F

GPLGSGKGCKVVVCGLLSVGKTAILEQLLYGNHTIGMEDCETMEDVYMASVETDRGVKEQLHLYDTRGLQEGVELPKHYFSFADGFVLVYSVNNLESFQRVELLKKEIDKFKDKKEVAIVVLGNKIDLSEQRQVDAEVAQQWAKSEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	;Escherichia coli 'BL21-Gold(DE3)pLysS AG' ;

Entity 3 non-polymer

description	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
formula_weight	522.2 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	32

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6. Citations (1)

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length_a	163.564 Å
length_b	63.430 Å
length_c	104.163 Å
angle_alpha	90.00 °
angle_beta	91.26 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	monoclinic

resolution	2.720 Å
resolution_low	63.630 Å
r_factor	0.1976
r_work	0.1951
r_free	0.2530

fit	/app/data/saxs_computation/qu/9qu1/9qu1.fit
pddf	/app/data/pr_computation/qu/9qu1/9qu1.pr.out
profile	/app/data/saxs_computation/qu/9qu1/9qu1.dat

Crystal structure of the human RalGAPA2 N-terminal domain with human kappaB-Ras1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)