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9r2n

Cryo-EM structure of the complex CCNY:14-3-3

Method: ELECTRON MICROSCOPY D_max: 102.5 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9r2n
deposition_date	2025-04-30
title	Cryo-EM structure of the complex CCNY:14-3-3
keywords	Kinase, Phosphorylation, Cell signalling, Cryo-EM, CYTOSOLIC PROTEIN; CYTOSOLIC PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	32.08 Å
rg_electron	31.49 Å
i0	110780000.00
molecular_weight	83059.0 kDa
excluded_volume	103900 Å³
envelope_volume	138020 Å³
shell_volume	36867 Å³
envelope_diameter	102.1 Å
shell_rg	38.13 Å
envelope_rg	31.08 Å
shape_rg	31.50 Å
total_rg	32.03 Å
total_atoms	11639
n_residues	717
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	102.5 Å
rg_real	32.02 Å
rg_real_error	0.70 Å
i0_real	1.1080e+08
i0_real_error	1.7480e+06
rg_reciprocal	32.05 Å
i0_reciprocal	110800000.0000
total_estimate	0.9063
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	1
r_peak_primary	35.6 Å
skewness	0.202
kurtosis	-0.648
angular_range	— – 0.2450 Å⁻¹
current_alpha	0.0000
highest_alpha	24050000.0000
n_real_points	50
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.944; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.993; Smooth: 0.954

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	14-3-3 protein eta
formula_weight	28446.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	11639

GHMGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 polymer

description	Cyclin-Y
formula_weight	39924.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

GPLGSMGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKRASCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRAASADNLTLPRWSPAIIS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.830 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.83 Å
resolution_method	—
num_particles	91774

fit	/app/data/saxs_computation/r2/9r2n/9r2n.fit
pddf	/app/data/pr_computation/r2/9r2n/9r2n.pr.out
profile	/app/data/saxs_computation/r2/9r2n/9r2n.dat

Cryo-EM structure of the complex CCNY:14-3-3

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)