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9rce

;Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the baseplate portion in extended state. ;

Method: ELECTRON MICROSCOPY D_max: 267.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9rce
deposition_date	2025-05-28
title	;Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the baseplate portion in extended state. ;
keywords	contractile injection system Salmonella, CONTRACTILE PROTEIN; CONTRACTILE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	72.48 Å
rg_electron	72.70 Å
i0	6872750000.00
molecular_weight	713220.0 kDa
excluded_volume	896640 Å³
envelope_volume	1533400 Å³
shell_volume	178320 Å³
envelope_diameter	277.9 Å
shell_rg	71.92 Å
envelope_rg	70.86 Å
shape_rg	72.68 Å
total_rg	72.75 Å
total_atoms	100103
n_residues	6322
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	267.2 Å
rg_real	76.55 Å
rg_real_error	1.69 Å
i0_real	6.9240e+09
i0_real_error	1.3240e+08
rg_reciprocal	72.01 Å
i0_reciprocal	6865000000.0000
total_estimate	0.8609
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	85.0 Å
skewness	0.645
kurtosis	0.218
angular_range	— – 0.1100 Å⁻¹
current_alpha	0.9782
highest_alpha	632000000.0000
n_real_points	23
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.717; Stabil: 0.854; Sysdev: 1.000; Positv: 1.000; Valcen: 0.976; Smooth: 0.539

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Peptidoglycan-binding protein
formula_weight	23868.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	H,M
atom_count	100103

MEKITIKSITSKGTVAQTFSATLNPNSIKHNFGISYTDKGPQQQGDISPTTVFKGYESEKLDFELLFDGTGVTGSTSTNTVQKQLATLKKVTYAYNGEQHEPNPVVIAWGSSVNFQGRLTAISINYSLFDSSGNPLRATVSLSFAKYLTQQEKSALKKQSSPDLTHIIEFKAGDTLPMLCHKIYNDSSYYIDVARANNLVSARQITPGTKIYFPPLI

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 2 polymer

description	Conserved hypothetical phage tail region protein
formula_weight	17506.8 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	I,N

MVTAKGSGAAQGDGDWPLPAFYFSVSISGCADDTAFQEVSGLEFQIETEEYREGGNNLVYHLPKTIKSSNLTLKRAVTDKASRLLSWCLDILNDELSKPIQTKSVSINLLNEKGTTCKSWTASNAYPVKLKMAPFNSTQNDIAIEEIEICFHHLKRNL

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 3 polymer

description	SalCis12
formula_weight	93034.2 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,J

MPAHSDWGVNSTMSKTISKKDHNDNPNSFGHLYQLGLTYIQQLSGHLWTDYNTHDPGMTILEQVCYALTDLIYRCEFEVTDYLSEPSGNIDYRAHGLALAEDIIPSYPQQPKEYEAWLLARLPELDKVWLRNSENDSHLGIYTLNAQLNHFYALSQQRAVTGKHSVYTQQYAALHRIRHEYYRVRAVGEDLAAIELTGQHPLSLSAVIHISDDVADVTWLAACIYHRIHLWLESNQQNTPVNVIKESLLAEDGILQIDRLEFMQHSDNVSHDEQKAIDNIAPFSYLMLPEASAHSGIEIVQFQHPVNIDYADLAIQIEQIQYQQRNAQLKTPTPPALPVGQYVDFTRYESIQTLFPRNYHLAPGTPIQYHAQQQAQRHQLRSYLLLFDQLMANFCDDIAGLNALFSLSLTPEVTYHAHSLQDDEFYNIEKHYPRDANAGLERLRAQLDNYPERKNRIFNYLLALYSERYPDWLHRQFNPYFSTQTLEKEILKYKQAFILNIVTMTNGRGIGDNLLQPEHQGGYCQRLALLLGLFPSVDAAKGNVRTFARYSLNLVSDQDYFHSDTGRKALWLTTLEAQTSLQPIEENALTGYPKESDIHDTLLTAPLFREKILPDALLQFGIDNHRYRLLSRQNHDVHQLFFHVDIDGHKRWFHIASHSDKQALILLCHQLQRWLVQLNRDSEELYVVEPILLRTEATSASLSDYANRVILVLPGYTARFSNLRFREQVEQLIVENSPAHLLTQCLWLDFAMFNQFETLYTQWRQAKSNALQHKERQPECDATAQRLYLFLQRASIGADGVESQ

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 4 polymer

description	GPW/gp25 family protein
formula_weight	16921.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,K

MANDNTFLGTGWAFPPRFTGPNHQVVMSSDMQDIEQSLTLLLSTTPGERLMTPDFGCRINQFVFEELTQTVLTQLSSEIRHAILFFESRIDVEDVHFLPEPLSGKLLIQIDYSVITTNTRSNMVYPFYLNEGTLISPQLLPLANEWDIA

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 5 polymer

description	Phage tail sheath family protein
formula_weight	57351.9 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	D,L

MGVMKTPGVYIVEQNAFPNSVVEVATAVPAFIGYTEKADNGGKSLSNKGWRITSMSEYRQYFGGEPQHLFEISEISTTSNANIREAFKQSGKTYQITQSNTRHHLYYSMLFFFQNGGGPCYIVSVGNYSDDIDAAVLKGGILPLIKEAEPTMLLIPEAIQLAEDDCINVEQAMLGHCGGKMKNRVAILDVWNGYKDRQHPDGDCVESFRSKLGTHYLDYAAAYYPWLNTSIVQDSDVSFLNISNIDKLAELLSGEVALMFSDLEGLSEEELSTGGNKLRATRKQAMLDEIAKLSAEISRPDAVLLHKILSNMSPLYQTIMADIKFQQNILPPSSAMAGIYTMVDNSRGVWKAPANVSVNAVVSPTVNISDDEQEDLNVTTQGKSINAIRPFIGEGTLVWGARTLDGNSVDWRYINVRRTMIMLEESIKLASKAYVFEPNVANTWVSMESMLSNFLYGIWKRGGLAGSTPGEAYNVSVGLGKTMTSNDILEGILRITVLVAMVRPAEFIEITFQQKMQES

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 6 polymer

description	SalCis6
formula_weight	6294.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

VPLEIKQLVIKSQTINDDNKSPSFGSDWPGIESEESVSAKVIPYPFMPSPDEVRER

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 7 polymer

description	Type VI secretion system tip protein VgrG
formula_weight	63170.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	F

MAISPLSNNSTGVTTYTIKVGGKAIDSAIGVIAIHIHYQVNHIATAEITISDGDMPTQRFDISDADTFKPGSTISISAGYASDEQTIFDGIIISHGIEIAANNSSSLSVICKDQAVAMTIAKHSQCFLGKSDSKIISTLLANYPNITASVGRITDPHSELVQFNSTDWDFILTRAEANGFVVTNQSNKVTVDKPAISNAADLVVTYGTDLISFSAKVDARNQLKSVTATAWDPAKQSMVTGTGPAQSISGQGNLTSSELAKVLGISDYTLQTASTLSTDSLTRWADGQQVKAMLSKVRGSVTFQGNASATINSLIELAGVGERYNGSHYISGVHHSIEKGQWITTAELGMSPMWSADHRDIGAPPASGYLPPVDGLQIGVVTKLDGDPESNYRIQIKIPTLNSEANVIWARLASYYASSGFGNFFIPEVGDEVIVGCINQDPSNPIILGSLYSSKNKMPEEMTSDNYIKTLVTKSKMKIIFDDENSVMTLKTPNGNTVVISDKNKSITLADQNSNTICMDKNGIAITSSKDVMISAKGGVNISSTRDTIVKATGDAKISGLNISAQANTGLTLKGTATAELSCSGITTVKGALVKIN

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 8 polymer

description	SalCis10
formula_weight	9573.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	c

MPFAARITDLQMCPMSTPGLPPIPHVGGPIIGPGVPTVLIGSLPAAVVGDSCVCVGPPSTIVKGSATVLIGGKPAARMGDTTSHGGSITIGCPTVIIGG

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

Entity 9 polymer

description	Baseplate protein J-like domain-containing protein
formula_weight	150611.3 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,G

MTSRQQRQDEAINSSLFHPHELSFEQLVGWAHQFAQQLPFRNLQDQADGHWGNLFQKSEIVVCAAISTSDTKHIQRQFKQALAHDENVTVEFLLILLKRLQAWYQHLPGAPETAYQFKYYLLHEYQRHLSLPLSLVICRLPEQFRHSVQELDPLWNLDQHTLDTASPVLQHSSFTEQMRHCFAKIVFVIEQIKKQSQQVLQAALMHQENNPQQALYFAFLKLFERAQQSLNQFTEKHLQFYYRQVLQQEKQSARENAVYLKLSLNHPTSSSIQFEQGAKFSPGDDPDFKPIAYRSRYPIEVTDAEVSHVFNLTLQRDPLVSPEKESGLVCGVSGQRVQITAGATGDDFPKAQQFNIFNNKYKTEDSTQPMGLIISDPLFSMQQGKRVIEIIVHLKEVRSSIVAQELLVVDDNNKTSAALTHIFAQLLSLHVHLFEDWAARITASGLTKQISQEQLSQFRQLRPSQRVWVAYKLFYLQTLQYICSTPEKGMQYGLSKIDLLFRIVGQMVSRRCLYTSAWLTKTDISTALSGLKSILVADPTAYTTIEELLSHSTTAAFYQIFQGGFDIEATTENGWELIDNVEIYPCPPQECQIGFKVKCHIDTGFAPIIPRLAHLPHSASLKITLKRQSNCFPYAIFRDFELSKLAMSTQVCGVTQLQLFNPEGQVDSSQPFFLFGSQPYMDAYVVLANEEIARKSISQLSLHLDWGNLPRGSDGFKQHYAEYHYPYTNASFQMRAEVLNNGRWVEFGPTGFSLFTPASGALRHDSHLHFLNMRNGYTPVTRPWPKTPYSNQSGLRNGLFKLLLTGPEPAFGHKDYAPLLSDTLTYNVTKKHKKTLPNQPYTPLVTHISIDYSAESTIDLLSVDRRSQSEIIHLYPFGENIIYPPKQTSPVHRPRFFPNYKEDSHCFIGITARELSGYLNIFFVFDGSARLVMPYPSTSYRWYYLVDNEWQALNPHQIIHDTTLNFLTTGIVTLDLPSEINTDHSVMPSGLFWLRVSTNKGIDRYPDCLHVATHVVKVTGKGVPLADDGITPLSFSSWRSTPRKANLAAIAQLNAMIRIPDIESEQHFQMRVSENLRHKGKALTPWDYEHLILENFPEVGSVHCFPTRSYYSLNQEPGRVLIIVTPLNTDCDHSLCSPKQLDSSYLLAIRRFLLSVSRSHVQIEVRNPGYEKIQIRCKVTLKEGVSHGPALRRLEYAIKAQLCPWEADTLNTGPGFCLSLEKLSAFILKQKNVVKVSALSALKISLDENTEYVLQDSAATSQPIRAAYPWFLLIPEEHQYIQISPDNLSHKPVTVGIGELVIGEQFIVSTSPTSPPKGAQNNG

Source

organism	Salmonella enterica subsp. salamae
ncbi_taxonomy_id	59202
host_organism	Escherichia coli

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.86 Å
resolution_method	—
num_particles	20065

fit	/app/data/saxs_computation/rc/9rce/9rce.fit
pddf	/app/data/pr_computation/rc/9rce/9rce.pr.out
profile	/app/data/saxs_computation/rc/9rce/9rce.dat

;Cryo-EM structure of a contractile injection system in Salmonella enterica subspecies Salamae, the baseplate portion in extended state. ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 polymer

Polymer

Source

Entity 7 polymer

Polymer

Source

Entity 8 polymer

Polymer

Source

Entity 9 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)