9rqi

Cryo-EM structure of Shigella flexneri LptDE bound by phage RBP reveals N-terminal strand insertion into lateral gate

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

LPS-assembly protein LptD

Shigella flexneri

UniProt Q83SQ0

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 LPS-assembly lipoprotein LptE × 1 (Q83LX4) Tail fiber protein × 1 (A0AAE8B389) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name LPTD_SHIFL
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 3–784; UniProt 3–784

LPS-assembly lipoprotein LptE

Shigella flexneri

UniProt Q83LX4

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 LPS-assembly protein LptD × 1 (Q83SQ0) Tail fiber protein × 1 (A0AAE8B389) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name LPTE_SHIFL
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B; PDBConstruct 1–193; UniProt 1–193

Tail fiber protein

Escherichia phage Oekolampad

UniProt A0AAE8B389

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 3 LPS-assembly protein LptD × 1 (Q83SQ0) LPS-assembly lipoprotein LptE × 1 (Q83LX4) Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name A0AAE8B389_9CAUD
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C; PDBConstruct 1–321; UniProt 1–321

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id9rqi
Deposition date deposition_date2025-06-25
Last revision last_revision2025-12-10
Structure title titleCryo-EM structure of Shigella flexneri LptDE bound by phage RBP reveals N-terminal strand insertion into lateral gate
Keywords keywordslipopolysaccharide transport, receptor-binding protein, outer membrane complex, beta-barrel, bacteriophage, MEMBRANE PROTEIN; MEMBRANE PROTEIN
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9rqi__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9rqi__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

9rqi__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)33.48 Å
Rg (electron density)33.30 Å
Total Rg33.97 Å
Atom count7899
Residues987
Excluded volume139000 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 9rqi__assembly_1__model_1 trimeric (3) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (3)

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7. Citations (1)