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9s1s

Crystal structure of C278S mutant of mouse CDC14A in complex with a model phosphopeptide

Method: X-RAY DIFFRACTION D_max: 103.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9s1s
deposition_date	2025-07-21
title	Crystal structure of C278S mutant of mouse CDC14A in complex with a model phosphopeptide
keywords	CDC14A, DUAL SPECIFICITY PHOSPHATASE, HEARING LOSS, STRUCTURAL-FUNCTIONAL RELATIONSHIP, CELL CYCLE; CELL CYCLE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.96 Å
rg_electron	30.57 Å
i0	96281700.00
molecular_weight	79616.0 kDa
excluded_volume	100370 Å³
envelope_volume	123470 Å³
shell_volume	34425 Å³
envelope_diameter	111.5 Å
shell_rg	36.56 Å
envelope_rg	30.69 Å
shape_rg	30.55 Å
total_rg	31.19 Å
total_atoms	11127
n_residues	681
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	103.8 Å
rg_real	31.02 Å
rg_real_error	0.76 Å
i0_real	9.6280e+07
i0_real_error	1.3660e+06
rg_reciprocal	31.00 Å
i0_reciprocal	96280000.0000
total_estimate	0.8809
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	32.5 Å
skewness	0.369
kurtosis	-0.403
angular_range	— – 0.2550 Å⁻¹
current_alpha	0.0000
highest_alpha	29330000.0000
n_real_points	52
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.874; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.911; Smooth: 0.914

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Dual specificity protein phosphatase CDC14A
formula_weight	39795.5 kDa
ec_number	3.1.3.16,3.1.3.48
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	11875

AAESGELIGACEFMKDRLYFATLRNRPKSTINIHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKKLKSYSLSRKKIVHYTSFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYNLTVLDCLQGIRKGLQHGFFDFETFDAEEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPEAYFPYFKKNNVTTIVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHSKAGLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLKEKQASLWVQGDIFRSKLKNR

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Baculovirus expression vector pFastBac1-HM

Entity 2 polymer

description	Model phosphohexapeptide
formula_weight	824.8 kDa
src_method	syn
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

ASPRRR

Entity 3 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	5

Entity 4 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	3

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	617

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6. Citations (1)

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length_a	102.342 Å
length_b	158.093 Å
length_c	69.274 Å
angle_alpha	90.00 °
angle_beta	124.59 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	monoclinic

resolution	1.620 Å
resolution_low	28.820 Å
r_factor	0.1698
r_work	0.1678
r_free	0.2067

fit	/app/data/saxs_computation/s1/9s1s/9s1s.fit
pddf	/app/data/pr_computation/s1/9s1s/9s1s.pr.out
profile	/app/data/saxs_computation/s1/9s1s/9s1s.dat

Crystal structure of C278S mutant of mouse CDC14A in complex with a model phosphopeptide

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 non-polymer

Entity 4 non-polymer

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)