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9shx

Type I-F_HNH variant Cascade bound to dsDNA, HNH domain in middle position

Method: ELECTRON MICROSCOPY D_max: 161.9 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9shx
deposition_date	2025-08-28
title	Type I-F_HNH variant Cascade bound to dsDNA, HNH domain in middle position
keywords	CRISPR-Cas Type I-F HNH nuclease, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	50.93 Å
rg_electron	50.66 Å
i0	1874490000.00
molecular_weight	343370.0 kDa
excluded_volume	421100 Å³
envelope_volume	580720 Å³
shell_volume	91224 Å³
envelope_diameter	158.9 Å
shell_rg	59.35 Å
envelope_rg	48.94 Å
shape_rg	50.68 Å
total_rg	50.84 Å
total_atoms	46740
n_residues	2790
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	161.9 Å
rg_real	50.74 Å
rg_real_error	1.29 Å
i0_real	1.8740e+09
i0_real_error	3.9640e+07
rg_reciprocal	51.07 Å
i0_reciprocal	1875000000.0000
total_estimate	0.8231
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	78.3 Å
skewness	0.015
kurtosis	-0.734
angular_range	— – 0.1550 Å⁻¹
current_alpha	0.0000
highest_alpha	242900000.0000
n_real_points	32
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.899; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.000

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4. Crystallography & Experiment

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5. Entities & Polymer Info (9)

Entity 1 polymer

description	Cas7f
formula_weight	38700.2 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	D,E,F,C,G,H
atom_count	46741

MAANKKATNVTLKSRPENLSFARCLNTTEAKFWQTDFLKRHTFKLPLLITDKAVLASKGHEMPPDKLEKEIMDPNPQKSQSCTLSTECDTLRIDFGIKVLPVKESMYSCSDYNYRTAIYQKIDEYIAEDGFLTLAKRYVNNIANARFLWRNRKGAEIIETIVTIEDKEYPSFNSKSFNLDTFVEDNATINEIAQQIADTFAGKREYLNIYVTCFVKIGCAMEVYPSQEMTFDDDDKGKKLFKFEGSAGMHSQKINNALRTIDTWYPDYTTYEFPIPVENYGAARSIGIPFRPDTKSFYKLIDRMILKNEDLPIEDKHYVMAILIRGGMFSKKQEK

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Cas5f
formula_weight	28703.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MMKGYILLEKVNIENANAFNNIIVGIPAITSFLGFARALERKLNAKEIAIRINGVGLEFHEYELKGYKNKRGQYVTSCPLPGSIPGQNEKKLDAHIMNQAYIDLNMSFLLEVEGPHVDMSTCKSIKSTMETLRIAGGIIRNYKKIRLIDTLADIPYGYFLTLRQDNLNDAAGDDMLDKMIHALQQEDTLVPIAVGFKALSEVGHVEGQRDPEKDHCFVESIFSLGGFECSKILEDINSCLWRYKTEEGLYLCTII

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	Cas8f fusion with HNH
formula_weight	43706.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MLRSKHHHHSGHHHTGHHHHSGSHHHTGGSGENLYFQGSGGLRNKILAAISQKIPEEQKINKYIEGLFQSIDKNHLATHVAKFTETNSPGNIGAYDILSSDMNCGYLDTANAGWKEPDIVTNDAKYKRPQGFVAMEMSDGRTVMEHLQEDSAELRHEMEELTDKYDEIRDGILNMPSMQPYRTNQFIKQVFFPVGGSYHLLSILPSTVLNYEVSDRLYRSKIPKIRLRLLSSNAASTTGSRLVSKNKWPLVFQALPPKFLEKNLAKALDKEYLLPDINIDELEGVDNGCLIDEALLPLIIDEGKRKGEGNYRPRHLRDERKEETVQAFLDKYGYCNIPVGYEVHHIVPLSQGGADSIKNMIMLSIEHHERVTEAHASYFKWRNT

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

description	Cas6f
formula_weight	20735.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MFSQILIIKPGTGISPNIIISEDIFPVLHSLFVEHDKKFGITFPAYSFDKKGHLGNIIEVLSEDKEALASLCLEEHLAEVTDYVKVKKEITFTDDYVLFKRIREENQYETTARRMRKRGHTELGRPLEMHIKKKNQQIFCHAYIKVKSASTGQSYNIFLAPTDIKHGSFSAYGLLRGDTHA

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 5 polymer

description	Non-target strand
formula_weight	14233.2 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	L

AAAAAGCGGAGAAGTCATTTAATAAGGCCACTGTTAAAAACCGTAC

Entity 6 polymer

description	Target strand
formula_weight	14062.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	K

GTACAATTTTTAACAGTGGCCTTATTAAATGACTTCTCCGCTTTTT

Entity 7 polymer

description	crRNA
formula_weight	19502.6 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	J

UUUAGAAGGAGAAGUCAUUUAAUAAGGCCACUGUUAAAAAGUGUACCGCCGGAUAGGCGX

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 8 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 9 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	1

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6. Citations (2)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	2.40 Å
resolution_method	—
num_particles	174646

fit	/app/data/saxs_computation/sh/9shx/9shx.fit
pddf	/app/data/pr_computation/sh/9shx/9shx.pr.out
profile	/app/data/saxs_computation/sh/9shx/9shx.dat

Type I-F_HNH variant Cascade bound to dsDNA, HNH domain in middle position

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (9)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Entity 7 polymer

Polymer

Source

Entity 8 non-polymer

Entity 9 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)