SAXSdb — 9sjo

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1. Structure Basics

entry_id	9sjo
deposition_date	2025-08-31
title	Type I-F_HNH variant Cascade target-free RNP, HNH domain in middle position
keywords	CRISPR-Cas Type I-F HNH nuclease, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	50.21 Å
rg_electron	49.80 Å
i0	1348510000.00
molecular_weight	299990.0 kDa
excluded_volume	371940 Å³
envelope_volume	521420 Å³
shell_volume	83665 Å³
envelope_diameter	149.0 Å
shell_rg	58.46 Å
envelope_rg	47.69 Å
shape_rg	49.82 Å
total_rg	49.99 Å
total_atoms	21047
n_residues	2500
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	153.3 Å
rg_real	49.99 Å
rg_real_error	1.17 Å
i0_real	1.3490e+09
i0_real_error	2.3530e+07
rg_reciprocal	50.39 Å
i0_reciprocal	1349000000.0000
total_estimate	0.8888
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	79.1 Å
skewness	-0.077
kurtosis	-0.786
angular_range	— – 0.1550 Å⁻¹
current_alpha	0.0000
highest_alpha	143600000.0000
n_real_points	32
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.896; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.997; Smooth: 0.866

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	Cas8f fusion with HNH
formula_weight	39339.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	21047

MLRNKILAAISQKIPEEQKINKYIEGLFQSIDKNHLATHVAKFTETNSPGNIGAYDILSSDMNCGYLDTANAGWKEPDIVTNDAKYKRPQGFVAMEMSDGRTVMEHLQEDSAELRHEMEELTDKYDEIRDGILNMPSMQPYRTNQFIKQVFFPVGGSYHLLSILPSTVLNYEVSDRLYRSKIPKIRLRLLSSNAASTTGSRLVSKNKWPLVFQALPPKFLEKNLAKALDKEYLLPDINIDELEGVDNGCLIDEALLPLIIDEGKRKGEGNYRPRHLRDERKEETVQAFLDKYGYCNIPVGYEVHHIVPLSQGGADSIKNMIMLSIEHHERVTEAHASYFKWRNT

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Cas5f
formula_weight	28703.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MMKGYILLEKVNIENANAFNNIIVGIPAITSFLGFARALERKLNAKEIAIRINGVGLEFHEYELKGYKNKRGQYVTSCPLPGSIPGQNEKKLDAHIMNQAYIDLNMSFLLEVEGPHVDMSTCKSIKSTMETLRIAGGIIRNYKKIRLIDTLADIPYGYFLTLRQDNLNDAAGDDMLDKMIHALQQEDTLVPIAVGFKALSEVGHVEGQRDPEKDHCFVESIFSLGGFECSKILEDINSCLWRYKTEEGLYLCTII

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	Cas6f
formula_weight	20735.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	I

MFSQILIIKPGTGISPNIIISEDIFPVLHSLFVEHDKKFGITFPAYSFDKKGHLGNIIEVLSEDKEALASLCLEEHLAEVTDYVKVKKEITFTDDYVLFKRIREENQYETTARRMRKRGHTELGRPLEMHIKKKNQQIFCHAYIKVKSASTGQSYNIFLAPTDIKHGSFSAYGLLRGDTHA

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

description	Cas7f
formula_weight	38700.2 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	D,E,F,C,G,H

MAANKKATNVTLKSRPENLSFARCLNTTEAKFWQTDFLKRHTFKLPLLITDKAVLASKGHEMPPDKLEKEIMDPNPQKSQSCTLSTECDTLRIDFGIKVLPVKESMYSCSDYNYRTAIYQKIDEYIAEDGFLTLAKRYVNNIANARFLWRNRKGAEIIETIVTIEDKEYPSFNSKSFNLDTFVEDNATINEIAQQIADTFAGKREYLNIYVTCFVKIGCAMEVYPSQEMTFDDDDKGKKLFKFEGSAGMHSQKINNALRTIDTWYPDYTTYEFPIPVENYGAARSIGIPFRPDTKSFYKLIDRMILKNEDLPIEDKHYVMAILIRGGMFSKKQEK

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

Entity 5 polymer

description	crRNA
formula_weight	19440.6 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	J

UUUAGAAGGAGAAGUCAUUUAAUAAGGCCACUGUUAAAAAGUGUACCGCCGGAUAGGCGG

Source

organism	Selenomonas sp.
ncbi_taxonomy_id	2053611
host_organism	Escherichia coli BL21(DE3)

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	triclinic

resolution	3.07 Å
resolution_method	—
num_particles	25866

fit	/app/data/saxs_computation/sj/9sjo/9sjo.fit
pddf	/app/data/pr_computation/sj/9sjo/9sjo.pr.out
profile	/app/data/saxs_computation/sj/9sjo/9sjo.dat

Type I-F_HNH variant Cascade target-free RNP, HNH domain in middle position

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)