← Back to search

9ssl

RAD51-ssDNA filament in complex with magnesium and ATP bound by the RAD54B N-terminus (peptide)

Method: ELECTRON MICROSCOPY D_max: 151.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9ssl
deposition_date	2025-09-26
title	RAD51-ssDNA filament in complex with magnesium and ATP bound by the RAD54B N-terminus (peptide)
keywords	RAD51 recombinase, RAD54B, filament modulation, homologous recombination, DNA BINDING PROTEIN; DNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	45.71 Å
rg_electron	45.72 Å
i0	819729000.00
molecular_weight	226870.0 kDa
excluded_volume	280070 Å³
envelope_volume	384830 Å³
shell_volume	69034 Å³
envelope_diameter	162.1 Å
shell_rg	51.01 Å
envelope_rg	45.38 Å
shape_rg	45.74 Å
total_rg	45.87 Å
total_atoms	15848
n_residues	2006
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	151.7 Å
rg_real	45.79 Å
rg_real_error	1.39 Å
i0_real	8.1970e+08
i0_real_error	1.7050e+07
rg_reciprocal	45.72 Å
i0_reciprocal	819600000.0000
total_estimate	0.8703
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	43.3 Å
skewness	0.314
kurtosis	-0.586
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	332700000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.865; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.940; Smooth: 0.775

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (6)

Entity 1 polymer

description	DNA repair protein RAD51 homolog 1
formula_weight	37009.1 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	15848

MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	DNA repair and recombination protein RAD54B
formula_weight	32036.6 kDa
ec_number	3.6.4.-
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	G,H,I,J,K,L

XMRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDLRICSLNLPSEESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSDSLVKYFSVVWCKPSKKKHKKWEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEEGQTLMICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSSKENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNCFPLVD

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Trichoplusia ni

Entity 3 polymer

description	DNA
formula_weight	6051.0 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	Z

CAACAACAACAACAACAACA

Entity 4 non-polymer

description	POTASSIUM ION
formula_weight	39.1 kDa
src_method	syn
entity_count	5

Entity 5 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	7

Entity 6 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	5

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.90 Å
resolution_method	—
num_particles	1258150

fit	/app/data/saxs_computation/ss/9ssl/9ssl.fit
pddf	/app/data/pr_computation/ss/9ssl/9ssl.pr.out
profile	/app/data/saxs_computation/ss/9ssl/9ssl.dat

RAD51-ssDNA filament in complex with magnesium and ATP bound by the RAD54B N-terminus (peptide)

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Entity 4 non-polymer

Entity 5 non-polymer

Entity 6 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)