← Back to search

9swn

Cryo-EM structure of yeast telomerase holoenzyme

Method: ELECTRON MICROSCOPY D_max: 223.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	9swn
deposition_date	2025-10-07
title	Cryo-EM structure of yeast telomerase holoenzyme
keywords	Telomerase, Telomerase RNA, RNA BINDING PROTEIN; RNA BINDING PROTEIN
method	ELECTRON MICROSCOPY

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	62.11 Å
rg_electron	61.15 Å
i0	2501090000.00
molecular_weight	373200.0 kDa
excluded_volume	447790 Å³
envelope_volume	749940 Å³
shell_volume	106030 Å³
envelope_diameter	217.7 Å
shell_rg	58.91 Å
envelope_rg	59.98 Å
shape_rg	61.06 Å
total_rg	61.37 Å
total_atoms	25945
n_residues	2804
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	223.7 Å
rg_real	62.41 Å
rg_real_error	2.64 Å
i0_real	2.5010e+09
i0_real_error	5.2360e+07
rg_reciprocal	61.83 Å
i0_reciprocal	2499000000.0000
total_estimate	0.8550
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	62.6 Å
skewness	0.418
kurtosis	-0.389
angular_range	— – 0.1250 Å⁻¹
current_alpha	0.0000
highest_alpha	201600000.0000
n_real_points	26
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.771; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.995; Smooth: 0.802

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (10)

Entity 1 polymer

description	Telomere replication protein EST3
formula_weight	20577.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	C
atom_count	25947

MPKVILESHSKPTDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKFYNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTNSRAMSHFKICLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL

Source

organism	Saccharomyces cerevisiae
common_name	;brewer's yeast ;
ncbi_taxonomy_id	4932
host_organism	Saccharomyces cerevisiae

Entity 2 polymer

description	Ribonucleases P/MRP protein subunit POP6
formula_weight	18235.0 kDa
ec_number	3.1.26.5
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	E

MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSIQYHGISKNDNIKKSVNKLDKQINMADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKGYIKNNKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ

Entity 3 polymer

description	Telomere elongation protein EST1
formula_weight	81920.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

MDNEEVNEECMRLFFKNARAHLDKHLTSRLTCDENAYITFRCFLDGIHRKSTRFLEELLLKQENMYHNNNYERINDSVIPLVLKLLWLQIHEPTLQWFEHWFHDIMRLSNRRKFRVFRIFQKKMIQFFKITHRYYYDIIEHLCAKYDMNSVISNALFAKLNLMQYTDGLSTHEKIILNTSNPLTFSIVISLQRCVINLGSTHFYKTLLNKPSNKPKSVEGFEKSIRYLNIASLYLPAVGDTYFQRAKIYLITGKFSLYFFELVRGALVRIPSKCALNNLKDFILTPDFPERRRLMKKLAILVSKDLKGEKSFFEGQIVLQFLSIVEHTLVPQSWNASRASNCWLLKEHLQMAALKYHSGNINVILENLAATMGSFDLMFTTRKSKEQKNKLKYADLSERQVFFLDLSFDFIANIIDVVIKPSWQKNMEDFRYLAIIRLLMCWIKSYRSILQYTHRHRKFCTSFALLLNDLINSPLNCSGNIYSHRPKRSYLFREDIIFREFSCINFALTDFNDDYVYDSPDMINNIIGCPTLTKVLSPKEECVLRIRSIIFSGMKFLEKNDTGVIWNASKYKFDLISPNIKIKRQIALSEISSKINVKTQQERVVSSRKVEAKRDEQQRKRAGKIAVTELEKQFANVRRTKKLSPLPEKDGVSSELVKHAASRGRKTITGPLSSDFLSYPDEAIDADEDITVQVPDTPT

Source

organism	Saccharomyces cerevisiae
common_name	;brewer's yeast ;
ncbi_taxonomy_id	4932
host_organism	Saccharomyces cerevisiae

Entity 4 polymer

description	Ribonucleases P/MRP protein subunit POP7
formula_weight	15844.3 kDa
ec_number	3.1.26.5
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	F

MALKKNTHNKSTKRVTKHPSLKTLTHKQIHTTIFVKSTTPYVSALKRINKFLDSVHKQGSSYVAVLGMGKAVEKTLALGCHFQDQKNKKIEVYTKTIEVLDEVITEGQADIDMESDVEDDDKETQLKKRAVSGVELRIYV

Entity 5 polymer

description	Ribonucleases P/MRP protein subunit POP1
formula_weight	100559.6 kDa
ec_number	3.1.26.5
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	D

MSGSLSRGNGGKKVLNKNQLLKRNRIRNARSIRAEAVAASSTKTGTPSDLSESGSKLNVDQFISSRQFEVKQLQLAMHNSKAASSTRIFQALPRKLRRRTASHNVRRIPKRMRNRALREMRKSDQQDVLKGSSASSRKAHGLNAKQLYKARMSIKLLRLASKSTSMKLSMPPEVTSSNCHVRQKIKTLKRMIKESSTANPNIKLLNNRMGSYDCTGVNELAPIPKGRVKYTKRQKHFAWLPTHIWNAKRSHMMKRWGYQMVWAPTQKCFKLTHRLGGDTCSSDGALCMDSSYIGTIIVKDKSNDSEGDFLKSIIGKLTAERANLRKYREGQVLFQGLIYSFNEENGEDSTKPLGPCDVFWVQKDTAIIRLHPSIYTQVFNILLQHKEKLTVQDCRYSLASVTLKGAKALESLASCLRSTEYSKSFEQFKMVSMITDHNALPQRCTFAFEAIDPRHLAAPKKLNDSQRKTVNSDDILSLHENYPQDEINAVFNELCDPESRTQSYNNQNTLKEISARRYKLLTATPNSINKTTVPFKESDDPSIPLVIIRRLKTRDWIVVLPWFWLLPLWHLLNRIPRMYHIGLRQFQQIQYENKQLYFPDDYPFTQLGYIENSFYKKEASKTKWDRKPMGKRINFEKIKDIHNTKLPAYSGEIGDFFSSDWRFLQILRNGIDYLQRNDKTLELMDSKKTGQFNAQGVRDINCVNDVLEFCKDYEAKTKAMSLSIEENIPVALCKNRKCQFRTPDSISVNSSSFSLTFFPRCIIAVSCTLLERGHPKDNARIYQVPEKDLEHWLQLAKGVYRPNGRKDHDLKIPLPEVHDLIGFITSGTYHLNCGNGMGIGFIDHHAAIRQPTRYVLIRNVGTNTYRLGEWSKISV

Entity 6 polymer

description	TLC1 RNA
formula_weight	370246.4 kDa
src_method	man
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B

GAGAGGAAGAUAGGUACCCUAUGAAAAUGUCAAUGGCUGUUGCGUUUGCUUAAUCGUAUUUUUUUUUUUUUCAGUCCGUGUUUUUUGUACAUUCUACGUUUGAGUUUUCCAUCAUGCAGGCCUCAGAAAUUUGGUAGGCACUCGAUGGUGAAGAGAUAGUGUCGGAUUUCGGAUUGAUCUUUCAGUUGAUAGCCUGCUGCUCUUUUCUUUUCCAAAGAAUUUCGAGUAUGCUGGUGUCAGUGUAGAUGCUUGUGUGUGCGCAAUUUGUGGUUUUUUAUUGUGUUUCUACUUAUAGAUGGCUAAAAUCUGAGUUUAGAAAAUGCAAACCGUAAAUUCUUAAACACUGCUAUUGCAUUUAGUUGCUAAAGCAGUGUUUUUGAACUUAUUCCUGUUAUUCCUUCUUCGUACCGAUCCUCUUCUCGACCUAACCUUUUAAUUACCAUGGGAAGCCUACCAUCACCACACCCACACACAAAUGUUACAGCUAAUUGUUUAUUAGCAAAGUUUGCACGAGUUCGCUGUUUAUUUUUUUCUCGUUUUCUUAUACCUAGUAUUUUUUCUGACACUGUUUAAGGUGACAGAAAAAAAGGAGUUUAAGUUAGAUUUGCAAACAGACGGUGCUAAGCGCUGUCACUUUAUGUCUAUCUUAUCGUUAACUCUGGAAAAAGAAAAAGGAAAAAGAACGUCAGGGAACAUGAGUAUAUAUAGAAAUGGUUUAUUCUAGUUUUUUCCGUUUUUUCAGUAGAUUUUUGCCUUUAAAAGAAUAAAUCCCACUACAAAAAGGUAAAAUAAAAAAUCUAUUCACUGAACUUACUGAUGAAAUUUCCAAAUGUGCCCCGUACAUCGAACGAUGUGACAGAGAAAAAUACGAGUAGGUAAAUAAGCCAAAAGGCAAGGGUGUCCUUUCUUAAGCAUCGGUUAGGUUUGCGGGCGAUCAGUAACUGAACAAUGACACAAGAUCAAGAACGUAAUUUGAGAUUUUUCAAGAUGGUUUUUUUAGGUAUCUAUUAAAACUACUUUGAUGAUCAAUACGGUAUUUUUGUCGCAUUAUUUUCCAAGCGGAAGGAACCGUGUGUUCAUUUUAUGAAUCUUGGUGUUGUAUUCACAGCUACUUCUCCUAAUGCCUUCGAUGCAUUUAGAUAAUUUUUGGAAACAUU

Source

organism	Saccharomyces cerevisiae
common_name	;brewer's yeast ;
ncbi_taxonomy_id	4932
host_organism	Saccharomyces cerevisiae

Entity 7 polymer

description	Telomerase reverse transcriptase
formula_weight	102768.1 kDa
ec_number	2.7.7.49
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MKILFEFIQDKLDIDLQTNSTYKENLKCGHFNGLDEILTTCFALPNSRKIALPCLPGDLSHKAVIDHCIIYLLTGELYNNVLTFGYKIARNEDVNNSLFCHSANVNVTLLKGAAWKMFHSLVGTYAFVDLLINYTVIQFNGQFFTQIVGNRCNEPHLPPKWAQRSSSSSATAAQIKQLTEPVTNKQFLHKLNINSSSFFPYSKILPSSSSIKKLTDLREAIFPTNLVKIPQRLKVRINLTLQKLLKRHKRLNYVSILNSICPPLEGTVLDLSHLSRQSPKERVLKFIIVILQKLLPQEMFGSKKNKGKIIKNLNLLLSLPLNGYLPFDSLLKKLRLKDFRWLFISDIWFTKHNFENLNQLAICFISWLFRQLIPKIIQTFFYCTEISSTVTIVYFRHDTWNKLITPFIVEYFKTYLVENNVCRNHNSYTLSNFNHSKMRIIPKKSNNEFRIIAIPCRGADEEEFTIYKENHKNAIQPTQKILEYLRNKRPTSFTKIYSPTQIADRIKEFKQRLLKKFNNVLPELYFMKFDVKSCYDSIPRMECMRILKDALKNENGFFVRSQYFFNTNTGVLKLFNVVNASRVPKPYELYIDNVRTVHLSNQDVINVVEMEIFKTALWVEDKCYIREDGLFQGSSLSAPIVDLVYDDLLEFYSEFKASPSQDTLILKLADDFLIISTDQQQVINIKKLAMGGFQKYNAKANRDKILAVSSQSDDDTVIQFCAMHIFVKELEVWKHSSTMNNFHIRSKSSKGIFRSLIALFNTRISYKTIDTNLNSTNTVLMQIDHVVKNISECYKSAFKDLSINVTQNMQFHSFLQRIIEMTVSGCPITKCDPLIEYEVRFTILNGFLESLSSNTSKFKDNIILLRKEIQHLQAYIYIYIHIVN

Source

organism	Saccharomyces cerevisiae
common_name	;brewer's yeast ;
ncbi_taxonomy_id	4932
host_organism	Saccharomyces cerevisiae

Entity 8 non-polymer

description	ZINC ION
formula_weight	65.4 kDa
src_method	syn
entity_count	2

Entity 9 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 10 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	2

▶

6. Citations (1)

▶

length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.000 Å
resolution_low	233.020 Å
r_factor	—
r_work	0.3294
r_free	—

resolution	3.00 Å
resolution_method	—
num_particles	125104

fit	/app/data/saxs_computation/sw/9swn/9swn.fit
pddf	/app/data/pr_computation/sw/9swn/9swn.pr.out
profile	/app/data/saxs_computation/sw/9swn/9swn.dat

Cryo-EM structure of yeast telomerase holoenzyme

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (10)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Entity 5 polymer

Polymer

Entity 6 polymer

Polymer

Source

Entity 7 polymer

Polymer

Source

Entity 8 non-polymer

Entity 9 non-polymer

Entity 10 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)