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9syt

;Asymmetric hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis with "long" coiled-coil domain ;

Method: ELECTRON MICROSCOPY D_max: 172.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9syt
deposition_date	2025-10-14
title	;Asymmetric hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis with "long" coiled-coil domain ;
keywords	RND superfamily, MmpL family, siderophore export, drug resistance, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	47.13 Å
rg_electron	46.76 Å
i0	970717000.00
molecular_weight	271550.0 kDa
excluded_volume	346000 Å³
envelope_volume	477150 Å³
shell_volume	85910 Å³
envelope_diameter	184.8 Å
shell_rg	51.46 Å
envelope_rg	45.82 Å
shape_rg	46.67 Å
total_rg	47.29 Å
total_atoms	38803
n_residues	2507
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	172.0 Å
rg_real	47.09 Å
rg_real_error	2.40 Å
i0_real	9.7070e+08
i0_real_error	2.0280e+07
rg_reciprocal	47.13 Å
i0_reciprocal	970800000.0000
total_estimate	0.8178
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	62.5 Å
skewness	0.396
kurtosis	0.229
angular_range	— – 0.1650 Å⁻¹
current_alpha	0.0000
highest_alpha	139200000.0000
n_real_points	34
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.574; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.908

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	Siderophore exporter MmpL4
formula_weight	105317.1 kDa
mutation	C39S, C319S, C335S, C393S, S434C, C780S
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	A,B,C
atom_count	38803

VSTKFANDSNTNARPEKPFIARMIHAFAVPIILGWLAVSVVVTVFVPSLEAVGQERSVSLSPKDAPSFEAMGRIGMVFKEGDSDSFAMVIIEGNQPLGDAAHKYYDGLVAQLRADKKHVQSVQDLWGDPLTAAGVQSNDGKAAYVQLSLAGNQGTPLANESVEAVRSIVESTPAPPGIKAYVTGPSALAADMHHSGDRSMARITMVTVAVIFIMLLLVYRSIITVVLLLITVGVELTAARGVVAVLGHSGAIGLTTFAVSLLTSLAIAAGTDYGIFIIGRYQEARQAGEDKEAAYYTMYRGTAHVILGSGLTIAGATFSLSFARMPYFQTLGIPSAVGMLVAVAVALTLGPAVLHVGSRFGLFDPKRLLKVRGWRRVGTVVVRWPLPVLVATSAIALVGLLALPGYKTSYNDRDYLPDFIPANQGYAAADRHFCQARMKPEILMIESDHDMRNPADFLVLDKLAKGIFRVPGISRVQAITRPEGTTMDHTSIPFQISMQNAGQLQTIKYQRDRANDMLKQADEMATTIAVLTRMHSLMAEMASTTHRMVGDTEEMKEITEELRDHVADFDDFWRPIRSYFYWEKHCYGIPICWSFRSIFDALDGIDKLSEQIGVLLGDLREMDRLMPQMVAQIPPQIEAMENMRTMILTMHSTMTGIFDQMLEMSDNATAMGKAFDAAKNDDSFYLPPEVFKNKDFQRAMKSFLSSDGHAARFIILHRGDPQSPEGIKSIDAIRTAAEESLKGTPLEDAKIYLAGTAAVFHDISEGAQWDLLIAAISSLSLIFIIMLIITRAFIAAAVIVGTVALSLGASFGLSVLLWQHILAIHLHWLVLAMSVIVLLAVGSDYNLLLVSRFKQEIGAGLKTGIIRSMGGTGKVVTNAGLVFAVTMASMAVSDLRVIGQVGTTIGLGLLFDTLIVRSFMTPSIAALLGRWFWWPLRVRSRPARTPTVPSETQPAGRPLAMSSDRLGA

Source

organism	Mycobacterium tuberculosis
ncbi_taxonomy_id	1773
host_organism	Escherichia coli MC1061

Entity 2 polymer

description	Siderophore export accessory protein MmpS4
formula_weight	15493.6 kDa
mutation	D39C
src_method	man
entity_count	3

Polymer

type	polypeptide(L)
strand_id	D,E,F

MSLMRTWIPLVILVVVIVGGFTVHRIRGFFGSENRPSYSCTNLENSKPFNPKHLTYEIFGPPGTVADISYFDVNSEPQRVDGAVLPWSLHITTNDAAVMGNIVAQGNSDSIGCRITVDGKVRAERVSNEVNAYTYCLVKSA

Source

organism	Mycobacterium tuberculosis
ncbi_taxonomy_id	1773
host_organism	Escherichia coli MC1061

Entity 3 non-polymer

description	DODECYL-BETA-D-MALTOSIDE
formula_weight	510.6 kDa
src_method	syn
entity_count	3

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.220 Å
resolution_low	— Å
r_factor	—
r_work	—
r_free	—

resolution	3.22 Å
resolution_method	—
num_particles	76903

fit	/app/data/saxs_computation/sy/9syt/9syt.fit
pddf	/app/data/pr_computation/sy/9syt/9syt.pr.out
profile	/app/data/saxs_computation/sy/9syt/9syt.dat

;Asymmetric hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis with "long" coiled-coil domain ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

EM 3D

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)