9u4m

Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT6 at top of spiral staircase

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

26S proteasome non-ATPase regulatory subunit 6

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD6_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain Y； PDBConstruct 1–389； UniProt 1–389

26S proteasome non-ATPase regulatory subunit 13

OrganismNot specified

UniProt Q9UNM6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSD13_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain a； PDBConstruct 1–376； UniProt 1–376

26S proteasome regulatory subunit 4

OrganismNot specified

UniProt P62191

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRS4_HUMAN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain B； PDBConstruct 1–440； UniProt 1–440

26S proteasome non-ATPase regulatory subunit 14

OrganismNot specified

UniProt O00487

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSDE_HUMAN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain c； PDBConstruct 1–309； UniProt 2–310

26S proteasome non-ATPase regulatory subunit 7

OrganismNot specified

UniProt P51665

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD7_HUMAN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain Z； PDBConstruct 1–324； UniProt 1–324

26S proteasome complex subunit SEM1

OrganismNot specified

UniProt P60896

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SEM1_HUMAN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain e； PDBConstruct 1–70； UniProt 1–70

26S proteasome non-ATPase regulatory subunit 1

OrganismNot specified

UniProt Q99460

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD1_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain U； PDBConstruct 1–953； UniProt 1–953

26S proteasome non-ATPase regulatory subunit 12

OrganismNot specified

UniProt O00232

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSD12_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain W； PDBConstruct 1–456； UniProt 1–456

26S proteasome regulatory subunit 7

OrganismNot specified

UniProt P35998

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRS7_HUMAN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain A； PDBConstruct 1–433； UniProt 1–433

26S proteasome regulatory subunit 8

OrganismNot specified

UniProt P62195

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRS8_HUMAN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain C； PDBConstruct 1–398； UniProt 9–406

26S proteasome regulatory subunit 6B

OrganismNot specified

UniProt P43686

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRS6B_HUMAN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain D； PDBConstruct 1–418； UniProt 1–418

Proteasome 26S subunit, ATPase 6

OrganismNot specified

UniProt A0A087X2I1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	A0A087X2I1_HUMAN
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain E； PDBConstruct 1–403； UniProt 1–403

26S proteasome regulatory subunit 6A

OrganismNot specified

UniProt P17980

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRS6A_HUMAN
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain F； PDBConstruct 1–439； UniProt 1–439

26S proteasome non-ATPase regulatory subunit 11

OrganismNot specified

UniProt O00231

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSD11_HUMAN
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain X； PDBConstruct 1–422； UniProt 1–422

26S proteasome non-ATPase regulatory subunit 3

OrganismNot specified

UniProt O43242

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD3_HUMAN
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain V； PDBConstruct 1–533； UniProt 2–534

26S proteasome non-ATPase regulatory subunit 8

OrganismNot specified

UniProt P48556

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 4 × 1 （P55036） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD8_HUMAN
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain d； PDBConstruct 1–349； UniProt 2–350

26S proteasome non-ATPase regulatory subunit 4

OrganismNot specified

UniProt P55036

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein heterocomplex	Heteromer	Protein 18	26S proteasome non-ATPase regulatory subunit 6 × 1 （Q15008） 26S proteasome non-ATPase regulatory subunit 13 × 1 （Q9UNM6） substrate peptide × 1 26S proteasome regulatory subunit 4 × 1 （P62191） 26S proteasome non-ATPase regulatory subunit 14 × 1 （O00487） 26S proteasome non-ATPase regulatory subunit 7 × 1 （P51665） 26S proteasome complex subunit SEM1 × 1 （P60896） 26S proteasome non-ATPase regulatory subunit 1 × 1 （Q99460） 26S proteasome non-ATPase regulatory subunit 12 × 1 （O00232） 26S proteasome regulatory subunit 7 × 1 （P35998） 26S proteasome regulatory subunit 8 × 1 （P62195） 26S proteasome regulatory subunit 6B × 1 （P43686） Proteasome 26S subunit, ATPase 6 × 1 （A0A087X2I1） 26S proteasome regulatory subunit 6A × 1 （P17980） 26S proteasome non-ATPase regulatory subunit 11 × 1 （O00231） 26S proteasome non-ATPase regulatory subunit 3 × 1 （O43242） 26S proteasome non-ATPase regulatory subunit 8 × 1 （P48556） ADENOSINE-5'-DIPHOSPHATE × 3 ZINC ION × 1 ADENOSINE-5'-TRIPHOSPHATE × 3 MAGNESIUM ION × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PSMD4_HUMAN
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain b； PDBConstruct 1–377； UniProt 1–377

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	9u4m
Deposition date deposition_date	2025-03-19
Structure title title	Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT6 at top of spiral staircase
Keywords keywords	Midnolin, 26S proteasome, Complex, HYDROLASE; HYDROLASE
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

9u4m__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

9u4m__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

9u4m__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	66.16 Å
Rg (electron density)	65.65 Å
Total Rg	65.74 Å
Atom count	46918
Residues	5894
Excluded volume	840610 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`9u4m__assembly_1__model_1`	18-meric （18）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (22)

Entity 1 Entity 1 polymer

Description description	26S proteasome non-ATPase regulatory subunit 6
Molecular weight formula_weight	45592.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y
Atom count atom_count	46918

MPLENLEEEGLPKNPDLRIAQLRFLLSLPEHRGDAAVRDELMAAVRDNNMAPYYEALCKSLDWQIDVDLLNKMKKANEDELKRLDEELEDAEKNLGESEIRDAMMAKAEYLCRIGDKEGALTAFRKTYDKTVALGHRLDIVFYLLRIGLFYMDNDLITRNTEKAKSLIEEGGDWDRRNRLKVYQGLYCVAIRDFKQAAELFLDTVSTFTSYELMDYKTFVTYTVYVSMIALERPDLREKVIKGAEILEVLHSLPAVRQYLFSLYECRYSVFFQSLAVVEQEMKKDWLFAPHYRYYVREMRIHAYSQLLESYRSLTLGYMAEAFGVGVEFIDQELSRFIAAGRLHCKIDKVNEIVETNRPDSKNWQYQETIKKGDLLLNRVQKLSRVINM

Entity 2 Entity 2 polymer

Description description	26S proteasome non-ATPase regulatory subunit 13
Molecular weight formula_weight	42995.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a

MKDVPGFLQQSQNSGPGQPAVWHRLEELYTKKLWHQLTLQVLDFVQDPCFAQGDGLIKLYENFISEFEHRVNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILCKTAIGALKLNIGDLQVTKETIEDVEEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNTDRQWLIDTLYAFNSGNVERFQTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFEEIAKSAKITVNEVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLEFWCTDVKSMEMLVEHQAHDILT

Entity 3 Entity 3 polymer

Description description	substrate peptide
Molecular weight formula_weight	586.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	v

AAAAAAAA

Entity 4 Entity 4 polymer

Description description	26S proteasome regulatory subunit 4
Molecular weight formula_weight	49260.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MGQSQSGGHGPGGGKKDDKDKKKKYEPPVPTRVGKKKKKTKGPDAASKLPLVTPHTQCRLKLLKLERIKDYLLMEEEFIRNQEQMKPLEEKQEEERSKVDDLRGTPMSVGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVVGSELIQKYLGDGPKLVRELFRVAEEHAPSIVFIDEIDAIGTKRYDSNSGGEREIQRTMLELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRMTLADDVTLDDLIMAKDDLSGADIKAICTEAGLMALRERRMKVTNEDFKKSKENVLYKKQEGTPEGLYL

Entity 5 Entity 5 polymer

Description description	26S proteasome non-ATPase regulatory subunit 14
Molecular weight formula_weight	34488.8 kDa
EC 编号 ec_number	3.4.19.-
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

DRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERAVAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKNELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDPKRHLEEHVDVLMTSNIVQCLAAMLDTVVFK

Entity 6 Entity 6 polymer

Description description	26S proteasome non-ATPase regulatory subunit 7
Molecular weight formula_weight	37086.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFLDHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYISVEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRSYLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKIANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEEKKEKK

Entity 7 Entity 7 polymer

Description description	26S proteasome complex subunit SEM1
Molecular weight formula_weight	8284.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MSEKKQPVDLGLLEEDDEFEEFPAEDWAGLDEDEDAHVWEDNWDDDNVEDDFSNQLRAELEKHGYKMETS

Entity 8 Entity 8 polymer

Description description	26S proteasome non-ATPase regulatory subunit 1
Molecular weight formula_weight	105958.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD

Entity 9 Entity 9 polymer

Description description	26S proteasome non-ATPase regulatory subunit 12
Molecular weight formula_weight	52979.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W

MADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAVVKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPIKLRLIDTLRMVTEGKIYVEIERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKRWKDLKNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGIINFQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ

Entity 10 Entity 10 polymer

Description description	26S proteasome regulatory subunit 7
Molecular weight formula_weight	48700.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MPDYLGADQRKTKEDEKDDKPIRALDEGDIALLKTYGQSTYSRQIKQVEDDIQQLLKKINELTGIKESDTGLAPPALWDLAADKQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

Entity 11 Entity 11 polymer

Description description	26S proteasome regulatory subunit 8
Molecular weight formula_weight	44852.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK

Entity 12 Entity 12 polymer

Description description	26S proteasome regulatory subunit 6B
Molecular weight formula_weight	47426.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

Entity 13 Entity 13 polymer

Description description	Proteasome 26S subunit, ATPase 6
Molecular weight formula_weight	45867.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MAIPGIPYERRLLIMADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFIVKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSEQIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGESARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATNRPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEAGMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV

Entity 14 Entity 14 polymer

Description description	26S proteasome regulatory subunit 6A
Molecular weight formula_weight	49266.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MNLLPNIESPVTRQEKMATVWDEAEQDGIGEEVLKMSTEEIIQRTRLLDSEIKIMKSEVLRVTHELQAMKDKIKENSEKIKVNKTLPYLVSNVIELLDVDPNDQEEDGANIDLDSQRKGKCAVIKTSTRQTYFLPVIGLVDAEKLKPGDLVGVNKDSYLILETLPTEYDSRVKAMEVDERPTEQYSDIGGLDKQIQELVEAIVLPMNHKEKFENLGIQPPKGVLMYGPPGTGKTLLARACAAQTKATFLKLAGPQLVQMFIGDGAKLVRDAFALAKEKAPSIIFIDELDAIGTKRFDSEKAGDREVQRTMLELLNQLDGFQPNTQVKVIAATNRVDILDPALLRSGRLDRKIEFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYYA

Entity 15 Entity 15 polymer

Description description	26S proteasome non-ATPase regulatory subunit 11
Molecular weight formula_weight	47526.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

Entity 16 Entity 16 polymer

Description description	26S proteasome non-ATPase regulatory subunit 3
Molecular weight formula_weight	60935.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

KQEGSARRRGADKAKPPPGGGEQEPPPPPAPQDVEMKEEAATGGGSTGEADGKTAAAAAEHSQRELDTVTLEDIKEHVKQLEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVQGFFTSNNATRDFLLPFLEEPMDTEADLQFRPRTGKAASTPLLPEVEAYLQLLVVIFMMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKCYYYHARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSEARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP

Entity 17 Entity 17 polymer

Description description	26S proteasome non-ATPase regulatory subunit 8
Molecular weight formula_weight	39536.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

FIKGRAPRAPPRERRRATRGGLRQVVAPPRALGSTSRPHFRRASVCRRRCRKSGGLLAASRKMAAAAVNGAAGFSSSGPAATSGAVLQAATGMYEQLKGEWNRKSPNLSKCGEELGRLKLVLLELNFLPTTGTKLTKQQLILARDILEIGAQWSILRKDIPSFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQTNVYIKHPVSLEQYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFTEATRILFFNTPKKMTDYAKKRGWVLGPNNYYSFASQQQKPEDTTIPSTELAKQVIEYARQLEMIV

Entity 18 Entity 18 polymer

Description description	26S proteasome non-ATPase regulatory subunit 4
Molecular weight formula_weight	40781.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b

MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

Entity 19 Entity 19 non-polymer

Description description	ADENOSINE-5'-DIPHOSPHATE
Molecular weight formula_weight	427.2 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 20 Entity 20 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 21 Entity 21 non-polymer

Description description	ADENOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	507.2 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 22 Entity 22 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	1

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	4.14 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	9022

Focused refinement of 19S in the substrate-engaged human 26S proteasome bound to midnolin with RPT6 at top of spiral staircase

1. Protein Identity and Related Structures Protein Identity & Related Structures

26S proteasome non-ATPase regulatory subunit 6

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome non-ATPase regulatory subunit 13

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome regulatory subunit 4

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome non-ATPase regulatory subunit 14

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome non-ATPase regulatory subunit 7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome complex subunit SEM1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome non-ATPase regulatory subunit 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome non-ATPase regulatory subunit 12

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome regulatory subunit 7

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

26S proteasome regulatory subunit 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer