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9uap

cryo-EM structure of ligand-free active-state M1 muscarinic acetylcholine receptor with alpha5 helix of G11 protein complex

Method: ELECTRON MICROSCOPY D_max: 87.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	9uap
deposition_date	2025-04-01
title	cryo-EM structure of ligand-free active-state M1 muscarinic acetylcholine receptor with alpha5 helix of G11 protein complex
keywords	GPCR, active-state, ligand-free, de novo protein, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	25.60 Å
rg_electron	25.17 Å
i0	24479600.00
molecular_weight	38395.0 kDa
excluded_volume	48345 Å³
envelope_volume	63395 Å³
shell_volume	22595 Å³
envelope_diameter	88.5 Å
shell_rg	30.66 Å
envelope_rg	25.53 Å
shape_rg	25.17 Å
total_rg	25.90 Å
total_atoms	2718
n_residues	399
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	87.0 Å
rg_real	25.82 Å
rg_real_error	0.74 Å
i0_real	2.4480e+07
i0_real_error	3.1560e+05
rg_reciprocal	25.75 Å
i0_reciprocal	24480000.0000
total_estimate	0.8342
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	23.9 Å
skewness	0.591
kurtosis	-0.166
angular_range	— – 0.3100 Å⁻¹
current_alpha	0.0000
highest_alpha	8385000.0000
n_real_points	63
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.721; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.851; Smooth: 0.827

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

Entity 1 polymer

description	M1 muscarinic acetylcholine receptor, de novo design protein
formula_weight	42070.7 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2718

VAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKRTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPVTVMCTLYWRIYRETKRAGERLAKLLEKFEALPLEDIVAALKALLATNRPEIQLAVKTIVENFPEIKKEAEKLTPEQKAKLAALEAQLADLPEELRKVLLSMYLTGLLYGGSEENRKRAIEKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCR

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	Guanine nucleotide-binding protein subunit alpha-11
formula_weight	3152.7 kDa
ec_number	3.6.5.-
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

TPENIRFVFAAVKDTILQLNLKEYNLV

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Spodoptera frugiperda

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6. Citations (1)

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length_a	1.000 Å
length_b	1.000 Å
length_c	1.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	—

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	3.62 Å
resolution_method	—
num_particles	155176

fit	/app/data/saxs_computation/ua/9uap/9uap.fit
pddf	/app/data/pr_computation/ua/9uap/9uap.pr.out
profile	/app/data/saxs_computation/ua/9uap/9uap.dat

cryo-EM structure of ligand-free active-state M1 muscarinic acetylcholine receptor with alpha5 helix of G11 protein complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

EM 3D

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)