SAXSdb — 9ust

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1. Structure Basics

entry_id	9ust
deposition_date	2025-05-02
title	MRGPRE-Gq-scFv16-complex
keywords	Complex, MEMBRANE PROTEIN, GPCR; MEMBRANE PROTEIN
method	ELECTRON MICROSCOPY

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	35.60 Å
rg_electron	35.21 Å
i0	363179000.00
molecular_weight	102940.0 kDa
excluded_volume	99554 Å³
envelope_volume	181220 Å³
shell_volume	43668 Å³
envelope_diameter	115.7 Å
shell_rg	40.64 Å
envelope_rg	35.45 Å
shape_rg	35.22 Å
total_rg	35.48 Å
total_atoms	7794
n_residues	1087
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	115.7 Å
rg_real	35.51 Å
rg_real_error	0.97 Å
i0_real	3.6320e+08
i0_real_error	6.1910e+06
rg_reciprocal	35.57 Å
i0_reciprocal	363200000.0000
total_estimate	0.8993
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	47.3 Å
skewness	0.164
kurtosis	-0.618
angular_range	— – 0.2200 Å⁻¹
current_alpha	0.0001
highest_alpha	32770000.0000
n_real_points	45
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.920; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.939

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4. Crystallography & Experiment

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5. Entities & Polymer Info (6)

Entity 1 polymer

description	Gq protein alpha subunit
formula_weight	41536.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	7794

CTLSAEDKAAVERSKMIEKQLQKDKQVYRRTLRLLLLGADNSGKSTIVKQMRIYHVNGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCSVDTENARRIFNDCKDIILQMNLREYNLV

Source

organism	Rattus norvegicus
ncbi_taxonomy_id	10116
host_organism	Spodoptera frugiperda

Entity 2 polymer

description	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
formula_weight	37784.3 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

GSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Spodoptera frugiperda

Entity 3 polymer

description	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
formula_weight	7729.9 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	G

ASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL

Source

organism	Bos taurus
common_name	domestic cattle
ncbi_taxonomy_id	9913
host_organism	Spodoptera frugiperda

Entity 4 polymer

description	MRGPRE
formula_weight	36350.8 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	R

AHEPRNDSMGVSPRPQPWPSHPNNGSELPTAANATAHATSVDGAHAFSAYENTLFLATVLVSLCGLVGNGTVIWLLGFRIKRNPFSVYILNLAGADFAFLFCKSVRFLLLVLNRSVAVLNVLIRGVTFSSYLGGLSLLMAVSVERCLSVLFPIWYRCRRLAQLSAIACALIWGLSLCMGILVFLCVHFVDFLCDVVNLVYNGMFFLTFLVLCASSLALLIWVQCFSMRRQPARLSRIVLLTVLAFLVLGLPLGAGLLADRLSPSLPCFDILLPILHLLSALNSGVNPLIYFFMGRQRQQRGQKPLREVLQSALTEDVELIREEPPSPDDT

Source

organism	Equus quagga
ncbi_taxonomy_id	89248
host_organism	Spodoptera frugiperda

Entity 5 polymer

description	scFv16
formula_weight	28782.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	S

MVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSAGGGGSGGGGSGGGGSSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELLEENLYFQGASHHHHHHHH

Source

organism	Bos taurus
ncbi_taxonomy_id	9913
host_organism	Spodoptera frugiperda

Entity 6 non-polymer

description	(~{E})-3-(2-methoxyphenyl)prop-2-enal
formula_weight	162.2 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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resolution	3.02 Å
resolution_method	—
num_particles	64460

fit	/app/data/saxs_computation/us/9ust/9ust.fit
pddf	/app/data/pr_computation/us/9ust/9ust.pr.out
profile	/app/data/saxs_computation/us/9ust/9ust.dat

MRGPRE-Gq-scFv16-complex

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

EM 3D

5. Entities & Polymer Info (6)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 polymer

Polymer

Source

Entity 6 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)